Peptides, Glycoconjugates and Metals in Biology

@article{Cois2024b,
title = {A Fluorogenic Chemogenetic pH Sensor for Imaging Protein Exocytosis},
author = {Justine Co{\"{i}}s and Marie-Laure Niepon and Manon Wittwer and Hessam {Sepasi Tehrani} and Philippe Bun and Jean-Maurice Mallet and Vincent Vialou and Blaise Dumat},
url = {https://pubs.acs.org/doi/10.1021/acssensors.4c01057},
doi = {10.1021/acssensors.4c01057},
issn = {2379-3694},
year = {2024},
date = {2024-08-01},
journal = {ACS Sensors},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

@article{Cois2024b,
title = {Design of Bright Chemogenetic Reporters Based on the Combined Engineering of Fluorogenic Molecular Rotors and of the HaloTag Protein},
author = {Justine Co{\"{i}}s and Sylvestre P J T Bachollet and Louis Sanchez and Nicolas Pietrancosta and Vincent Vialou and Jean Maurice Mallet and Blaise Dumat},
url = {https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/chem.202400641},
doi = {10.1002/chem.202400641},
issn = {15213765},
year = {2024},
date = {2024-06-01},
journal = {Chemistry - A European Journal},
volume = {30},
number = {32},
pages = {e202400641},
abstract = {The combination of fluorogenic probes (fluorogens) and self-labeling protein tags represent a promising tool for imaging biological processes with high specificity but it requires the adequation between the fluorogen and its target to ensure a good activation of its fluorescence. In this work, we report a strategy to develop molecular rotors that specifically target HaloTag with a strong enhancement of their fluorescence. The divergent design facilitates the diversification of the structures to tune the photophysical and cellular properties. Four bright fluorogens with emissions ranging from green to red were identified and applied in wash-free live cell imaging experiments with good contrast and selectivity. A HaloTag mutant adapted from previous literature reports was also tested and shown to further improve the brightness and reaction rate of the most promising fluorogen of the series both in vitro and in cells. This work opens new possibilities to develop bright chemogenetic reporters with diverse photophysical and biological properties by exploring a potentially large chemical space of simple dipolar fluorophores in combination with protein engineering.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

@article{Michelis2024,
title = {FRET-Sensing of Multivalent Protein Binding at the Interface of Biomimetic Microparticles Functionalized with Fluorescent Glycolipids},
author = {Sophie Michelis and Chiara Pompili and Florence Niedergang and Jacques Fattaccioli and Blaise Dumat and Jean-Maurice Mallet},
url = {https://pubs.acs.org/doi/10.1021/acsami.3c15067},
doi = {10.1021/acsami.3c15067},
issn = {1944-8244},
year = {2024},
date = {2024-02-01},
journal = {ACS Applied Materials & Interfaces},
volume = {16},
number = {8},
pages = {9669--9679},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

@article{SCHANNE2024215906,
title = {Cellular evaluation of superoxide dismutase mimics as catalytic drugs: Challenges and opportunities},
author = {Gabrielle Schanne and Sylvie Demignot and Clotilde Policar and Nicolas Delsuc},
url = {https://www.sciencedirect.com/science/article/pii/S0010854524002522},
doi = {https://doi.org/10.1016/j.ccr.2024.215906},
issn = {0010-8545},
year = {2024},
date = {2024-01-01},
journal = {Coordination Chemistry Reviews},
volume = {514},
pages = {215906},
abstract = {Oxidative stress is known to be associated with many pathologies including inflammation, cancer, diabetes, etc. However, oxidative stress resulting from the imbalance between reactive oxygen species flows and antioxidant defenses has been largely overlooked so far as a therapeutic target. Among antioxidant defenses, superoxide dismutases (SOD) are metalloenzymes that catalyze efficiently the dismutation of superoxide, the first reactive oxygen species resulting from the monoelectronic reduction of dioxygen. Superoxide, as a quite reactive chemical species, is a transient species. So, the cellular evaluation of metal complexes mimicking SOD (SOD mimics) in cellular models can be particularly tedious and calls for multiple direct and indirect strategies including probes and biochemical assays. This review highlights methods and assays to evaluate in cells SOD mimics, a new class of catalytic antioxidants.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

@article{CRCHIM_2024__27_S2_A12_0,
title = {Metal complexes in cells: from design of catalytic antioxidants to imaging metal ions and designing metal-based probes in X-ray fluorescence and IR-imaging, a multidisciplinary collaborative journey in bioinorganic chemistry and inorganic chemical biology},
author = {Clotilde Policar and Nicolas Delsuc and H\'{e}l`ene Charlotte Bertrand},
doi = {10.5802/crchim.295},
year = {2024},
date = {2024-01-01},
journal = {Comptes Rendus. Chimie},
publisher = {Acad\'{e}mie des sciences, Paris},
note = {Online first},
keywords = {},
pubstate = {published},
tppubtype = {article}
}