Bioinorganic Chemistry and Redox Homeostasis
Our website :
Metals in Biology and Redox Homeostasis
A new name for our research group: METROX
CONGRATULATIONS to our former PhD students:
Jean Bouvet for his selection to the MBA « collège des ingénieurs » (jan. 2024)
Paul Demay-Drouhard, who was appointed as a CNRS researcher (section 12, ICOA Orléans) in 2023
Martha Zoumpoulaki for her selection to the MBA « collège des ingénieurs » (oct. 2021) and her recruitment at Air Liquide in 2023.
Koudedja Coulibaly, who was recruited by Air Liquide in 2021
Emilie Mathieu, who was appointed as a CNRS researcher (section 16, LCC Toulouse) in 2021
Sarah Hostachy, who was appointed as a CEAEA researcher (LCBM, Grenoble) in 2020
Our personal webpages and resumes:
Alice Balfourier (ORCID: 0000-0002-4801-1388)
Hélène Bertrand (ORCID: 0000-0002-3841-022X)
Nicolas Delsuc (ORCID: 0000-0001-5570-8311)
Clotilde Policar (ORCID: 0000-0003-0255-1650)
Christine Rampon (ORCID: 0000-0002-1444-3166)
Michel Volovitch (ORCID: 0000-0002-7488-764X)
Sophie Vriz
Some news about our work:
About our work and equity in science (in French): https://www.youtube.com/watch?v=ZfyFIkh_G4k
https://www.inc.cnrs.fr/fr/cnrsinfo/des-complexes-bio-inspires-dans-le-vent
https://www.ens.psl.eu/actualites/des-catalyseurs-bio-inspires-pour-lutter-contre-le-stress-oxydant
Publications of the group:
((Go back to the publication page of the ens-bic website))
2025 |
A tunable and versatile chemogenetic near-infrared fluorescent reporter Article de journal Lina El Hajji; Benjamin Bunel; Octave Joliot; Chenge Li; Alison G Tebo; Christine Rampon; Michel Volovitch; Evelyne Fischer; Nicolas Pietrancosta; Franck Perez; Xavier Morin; Sophie Vriz; Arnaud Gautier Nature Communications, 16 (1), p. 2594, 2025, ISSN: 2041-1723. @article{el_hajji_tunable_2025, title = {A tunable and versatile chemogenetic near-infrared fluorescent reporter}, author = {Lina El Hajji and Benjamin Bunel and Octave Joliot and Chenge Li and Alison G Tebo and Christine Rampon and Michel Volovitch and Evelyne Fischer and Nicolas Pietrancosta and Franck Perez and Xavier Morin and Sophie Vriz and Arnaud Gautier}, url = {https://doi.org/10.1038/s41467-025-58017-9}, doi = {10.1038/s41467-025-58017-9}, issn = {2041-1723}, year = {2025}, date = {2025-01-01}, journal = {Nature Communications}, volume = {16}, number = {1}, pages = {2594}, abstract = {Near-infrared (NIR) fluorescent reporters open interesting perspectives for multiplexed imaging with higher contrast and depth using less toxic light. Here, we propose nirFAST, a small (14 kDa) chemogenetic NIR fluorescent reporter, displaying higher cellular brightness compared to top-performing NIR fluorescent proteins. nirFAST binds and stabilizes the fluorescent state of synthetic cell permeant fluorogenic chromophores (so-called fluorogens), otherwise dark when free. nirFAST displays tunable NIR, far-red or red emission through change of fluorogen. nirFAST allows imaging and spectral multiplexing in live cultured mammalian cells, chicken embryo tissues and zebrafish larvae. Its suitability for stimulated emission depletion nanoscopy enabled protein imaging with subdiffraction resolution in live cells. nirFAST enabled the design of a two-color cell cycle indicator for monitoring the different phases of the cell cycle. Finally, bisection of nirFAST allowed the design of a chemically induced dimerization technology with NIR fluorescence readout, enabling the control and visualization of protein proximity.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Near-infrared (NIR) fluorescent reporters open interesting perspectives for multiplexed imaging with higher contrast and depth using less toxic light. Here, we propose nirFAST, a small (14 kDa) chemogenetic NIR fluorescent reporter, displaying higher cellular brightness compared to top-performing NIR fluorescent proteins. nirFAST binds and stabilizes the fluorescent state of synthetic cell permeant fluorogenic chromophores (so-called fluorogens), otherwise dark when free. nirFAST displays tunable NIR, far-red or red emission through change of fluorogen. nirFAST allows imaging and spectral multiplexing in live cultured mammalian cells, chicken embryo tissues and zebrafish larvae. Its suitability for stimulated emission depletion nanoscopy enabled protein imaging with subdiffraction resolution in live cells. nirFAST enabled the design of a two-color cell cycle indicator for monitoring the different phases of the cell cycle. Finally, bisection of nirFAST allowed the design of a chemically induced dimerization technology with NIR fluorescence readout, enabling the control and visualization of protein proximity. |
Angle-dependent spin crossover properties in polymorphic iron (ii) complexes based on pyridine--triazole derivatives Article de journal Emmelyne Cuza; Nicolas Delsuc; Jer^ome Marrot; William Shepard; Clotilde Policar; Christian Serre; Antoine Tissot Dalton Transactions, 54 (15), p. 6274–6280, 2025. @article{cuza2025angled, title = {Angle-dependent spin crossover properties in polymorphic iron (ii) complexes based on pyridine--triazole derivatives}, author = {Emmelyne Cuza and Nicolas Delsuc and Jer{^o}me Marrot and William Shepard and Clotilde Policar and Christian Serre and Antoine Tissot}, year = {2025}, date = {2025-01-01}, journal = {Dalton Transactions}, volume = {54}, number = {15}, pages = {6274--6280}, publisher = {Royal Society of Chemistry}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Angle-dependent spin crossover properties in polymorphic iron (ii) complexes based on pyridine--triazole derivatives Article de journal Emmelyne Cuza; Nicolas Delsuc; Jer^ome Marrot; William Shepard; Clotilde Policar; Christian Serre; Antoine Tissot Dalton Transactions, 54 (15), p. 6274–6280, 2025. @article{cuza2025anglec, title = {Angle-dependent spin crossover properties in polymorphic iron (ii) complexes based on pyridine--triazole derivatives}, author = {Emmelyne Cuza and Nicolas Delsuc and Jer{^o}me Marrot and William Shepard and Clotilde Policar and Christian Serre and Antoine Tissot}, year = {2025}, date = {2025-01-01}, journal = {Dalton Transactions}, volume = {54}, number = {15}, pages = {6274--6280}, publisher = {Royal Society of Chemistry}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Angle-dependent spin crossover properties in polymorphic iron (ii) complexes based on pyridine--triazole derivatives Article de journal Emmelyne Cuza; Nicolas Delsuc; Jer^ome Marrot; William Shepard; Clotilde Policar; Christian Serre; Antoine Tissot Dalton Transactions, 54 (15), p. 6274–6280, 2025. @article{cuza2025angleb, title = {Angle-dependent spin crossover properties in polymorphic iron (ii) complexes based on pyridine--triazole derivatives}, author = {Emmelyne Cuza and Nicolas Delsuc and Jer{^o}me Marrot and William Shepard and Clotilde Policar and Christian Serre and Antoine Tissot}, year = {2025}, date = {2025-01-01}, journal = {Dalton Transactions}, volume = {54}, number = {15}, pages = {6274--6280}, publisher = {Royal Society of Chemistry}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Angle-dependent spin crossover properties in polymorphic iron (ii) complexes based on pyridine--triazole derivatives Article de journal Emmelyne Cuza; Nicolas Delsuc; Jer^ome Marrot; William Shepard; Clotilde Policar; Christian Serre; Antoine Tissot Dalton Transactions, 54 (15), p. 6274–6280, 2025. @article{cuza2025angle, title = {Angle-dependent spin crossover properties in polymorphic iron (ii) complexes based on pyridine--triazole derivatives}, author = {Emmelyne Cuza and Nicolas Delsuc and Jer{^o}me Marrot and William Shepard and Clotilde Policar and Christian Serre and Antoine Tissot}, year = {2025}, date = {2025-01-01}, journal = {Dalton Transactions}, volume = {54}, number = {15}, pages = {6274--6280}, publisher = {Royal Society of Chemistry}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kinetic Redox Shotgun Proteomics Reveals Specific Lipopolysaccharide Effects on Intestinal Epithelial Cells, Mitigated by a Mn Superoxide Dismutase Mimic Article de journal Martha Zoumpoulaki; Giovanni Chiappetta; Jean Bouvet; Namita-Raju John; Gabrielle Schanne; Pauline Gehan; Samuel Diebolt; Shakir Shakir; Elodie Quévrain; Emilie Mathieu; Sylvie Demignot; Philippe Seksik; Nicolas Delsuc; Joelle Vinh; Clotilde Policar Angewandte Chemie International Edition, n/a (n/a), p. e202422644, 2025. @article{https://doi.org/10.1002/anie.202422644, title = {Kinetic Redox Shotgun Proteomics Reveals Specific Lipopolysaccharide Effects on Intestinal Epithelial Cells, Mitigated by a Mn Superoxide Dismutase Mimic}, author = {Martha Zoumpoulaki and Giovanni Chiappetta and Jean Bouvet and Namita-Raju John and Gabrielle Schanne and Pauline Gehan and Samuel Diebolt and Shakir Shakir and Elodie Qu\'{e}vrain and Emilie Mathieu and Sylvie Demignot and Philippe Seksik and Nicolas Delsuc and Joelle Vinh and Clotilde Policar}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.202422644}, doi = {https://doi.org/10.1002/anie.202422644}, year = {2025}, date = {2025-01-01}, journal = {Angewandte Chemie International Edition}, volume = {n/a}, number = {n/a}, pages = {e202422644}, abstract = {Abstract Overproduction of reactive oxygen species and antioxidant superoxide dismutases (SOD1, SOD2) dysregulation contribute to chronic inflammation such as generated in inflammatory bowel diseases (IBD). A kinetic redox shotgun proteomic strategy (OcSILAC for Oxidized cysteine Stable Isotope Labelling by Amino acids in Cell culture) was used to explore the lipopolysaccharide (LPS) effects including LPS-induced oxidation and inflammation cascades on a dedicated intestinal epithelial cell line (HT29-MD2) together with the potential mitigating role of a Mn-based SOD-mimic Mn1. While LPS induced transient oxidative damages at early times (15 min), cells incubated with Mn1 showed, in this time frame, a significantly reduced cysteine oxidation, highlighting Mn1 antioxidant properties. Over time, cysteine oxidation of LPS-treated cells was counteracted by an overexpression of antioxidant proteins (SOD1, NQO1) and a late (6 h) preponderant increase in SOD2 level. Mn1, when co-incubated with LPS, attenuated the level of most LPS-modified proteins, that is, proteins involved in the inflammatory response. Our results highlight Mn1 as a potentially effective antioxidant and anti-inflammatory agent to consider in the treatment of IBD, as well as a useful tool for exploring the interconnection between oxidative stress and inflammation.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Overproduction of reactive oxygen species and antioxidant superoxide dismutases (SOD1, SOD2) dysregulation contribute to chronic inflammation such as generated in inflammatory bowel diseases (IBD). A kinetic redox shotgun proteomic strategy (OcSILAC for Oxidized cysteine Stable Isotope Labelling by Amino acids in Cell culture) was used to explore the lipopolysaccharide (LPS) effects including LPS-induced oxidation and inflammation cascades on a dedicated intestinal epithelial cell line (HT29-MD2) together with the potential mitigating role of a Mn-based SOD-mimic Mn1. While LPS induced transient oxidative damages at early times (15 min), cells incubated with Mn1 showed, in this time frame, a significantly reduced cysteine oxidation, highlighting Mn1 antioxidant properties. Over time, cysteine oxidation of LPS-treated cells was counteracted by an overexpression of antioxidant proteins (SOD1, NQO1) and a late (6 h) preponderant increase in SOD2 level. Mn1, when co-incubated with LPS, attenuated the level of most LPS-modified proteins, that is, proteins involved in the inflammatory response. Our results highlight Mn1 as a potentially effective antioxidant and anti-inflammatory agent to consider in the treatment of IBD, as well as a useful tool for exploring the interconnection between oxidative stress and inflammation. |
SOD mimics delivered to the gut using lactic acid bacteria mitigate the colitis symptoms in a mouse model of Inflammatory Bowel Diseases Article de journal Gabrielle Schanne; Amandine Vincent; Florian Chain; Pauline Ruffié; Célia Carbonne; Elodie Quévrain; Emilie Mathieu; Alice Balfourier; Luis G Bermúdez-Humarán; Philippe Langella; Sophie Thenet; Véronique Carrière; Nassim Hammoudi; Magali Svreck; Sylvie Demignot; Philippe Seksik; Clotilde Policar; Nicolas Delsuc Free Radical Research, p. 1–16, 2025, (PMID: 40079422). @article{Schanne13032025, title = {SOD mimics delivered to the gut using lactic acid bacteria mitigate the colitis symptoms in a mouse model of Inflammatory Bowel Diseases}, author = {Gabrielle Schanne and Amandine Vincent and Florian Chain and Pauline Ruffi\'{e} and C\'{e}lia Carbonne and Elodie Qu\'{e}vrain and Emilie Mathieu and Alice Balfourier and Luis G Berm\'{u}dez-Humar\'{a}n and Philippe Langella and Sophie Thenet and V\'{e}ronique Carri\`{e}re and Nassim Hammoudi and Magali Svreck and Sylvie Demignot and Philippe Seksik and Clotilde Policar and Nicolas Delsuc}, url = {https://doi.org/10.1080/10715762.2025.2478121}, doi = {10.1080/10715762.2025.2478121}, year = {2025}, date = {2025-01-01}, journal = {Free Radical Research}, pages = {1--16}, publisher = {Taylor & Francis}, note = {PMID: 40079422}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2024 |
Multiplexed In Vivo Imaging with Fluorescence Lifetime-Modulating Tags Article de journal Lina El Hajji; France Lam; Maria Avtodeeva; Hela Benaissa; Christine Rampon; Michel Volovitch; Sophie Vriz; Arnaud Gautier Advanced Science, 11 (32), p. 2404354, 2024. @article{https://doi.org/10.1002/advs.202404354, title = {Multiplexed In Vivo Imaging with Fluorescence Lifetime-Modulating Tags}, author = {Lina El Hajji and France Lam and Maria Avtodeeva and Hela Benaissa and Christine Rampon and Michel Volovitch and Sophie Vriz and Arnaud Gautier}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202404354}, doi = {https://doi.org/10.1002/advs.202404354}, year = {2024}, date = {2024-01-01}, journal = {Advanced Science}, volume = {11}, number = {32}, pages = {2404354}, abstract = {Abstract Fluorescence lifetime imaging microscopy (FLIM) opens new dimensions for highly multiplexed imaging in live cells and organisms using differences in fluorescence lifetime to distinguish spectrally identical fluorescent probes. Here, a set of fluorescence-activating and absorption-shifting tags (FASTs) capable of modulating the fluorescence lifetime of embedded fluorogenic 4-hydroxybenzylidene rhodanine (HBR) derivatives is described. It is shown that changes in the FAST protein sequence can vary the local environment of the chromophore and lead to significant changes in fluorescence lifetime. These fluorescence lifetime-modulating tags enable multiplexed imaging of up to three targets in one spectral channel using a single HBR derivative in live cells and live zebrafish larvae. The combination of fluorescence lifetime multiplexing with spectral multiplexing allows to successfully image six targets in live cells, opening great prospects for multicolor fluorescence lifetime multiplexing.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Fluorescence lifetime imaging microscopy (FLIM) opens new dimensions for highly multiplexed imaging in live cells and organisms using differences in fluorescence lifetime to distinguish spectrally identical fluorescent probes. Here, a set of fluorescence-activating and absorption-shifting tags (FASTs) capable of modulating the fluorescence lifetime of embedded fluorogenic 4-hydroxybenzylidene rhodanine (HBR) derivatives is described. It is shown that changes in the FAST protein sequence can vary the local environment of the chromophore and lead to significant changes in fluorescence lifetime. These fluorescence lifetime-modulating tags enable multiplexed imaging of up to three targets in one spectral channel using a single HBR derivative in live cells and live zebrafish larvae. The combination of fluorescence lifetime multiplexing with spectral multiplexing allows to successfully image six targets in live cells, opening great prospects for multicolor fluorescence lifetime multiplexing. |
Editorial: Insights in developmental endocrinology: 2023 Article de journal Lawrence M Nelson; Mayank Choubey; Hiroyasu Kamei; Christine Rampon Frontiers in Endocrinology, 15 , 2024. @article{doi:10.3389/fendo.2024.1453023, title = {Editorial: Insights in developmental endocrinology: 2023}, author = {Lawrence M Nelson and Mayank Choubey and Hiroyasu Kamei and Christine Rampon}, url = {https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2024.1453023}, doi = {10.3389/fendo.2024.1453023}, year = {2024}, date = {2024-01-01}, journal = {Frontiers in Endocrinology}, volume = {15}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Piezoelectric and microfluidic tuning of an infrared cavity for vibrational polariton studies Article de journal Wei Wang; Jaime de la Fuente Diez; Nicolas Delsuc; Juan Peng; Riccardo Spezia; Rodolphe Vuilleumier; Yong Chen Lab Chip, p. -, 2024. @article{D3LC01101A, title = {Piezoelectric and microfluidic tuning of an infrared cavity for vibrational polariton studies}, author = {Wei Wang and Jaime de la Fuente Diez and Nicolas Delsuc and Juan Peng and Riccardo Spezia and Rodolphe Vuilleumier and Yong Chen}, url = {http://dx.doi.org/10.1039/D3LC01101A}, doi = {10.1039/D3LC01101A}, year = {2024}, date = {2024-01-01}, journal = {Lab Chip}, pages = {-}, publisher = {The Royal Society of Chemistry}, abstract = {We developed a microfluidic system for vibrational polariton studies, which consists of two microfluidic chips: one for solution mixing and another for tuning an infrared cavity made of a pair of gold mirrors and a PDMS (polydimethylsiloxane) spacer. We show that the cavity of the system can be accurately tuned with either piezoelectric actuators or microflow-induced pressure to result in resonant coupling between a cavity mode and a variational mode of the solution molecules. Acrylonitrile solutions were chosen to prove the concept of vabriational strong coupling (VSC) of a CN stretching mode with light inside the cavity. We also show that the Rabi splitting energy is linearly proportional to the square root of molecular concentration, thereby proving the relevance and reliability of the system for VSC studies.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We developed a microfluidic system for vibrational polariton studies, which consists of two microfluidic chips: one for solution mixing and another for tuning an infrared cavity made of a pair of gold mirrors and a PDMS (polydimethylsiloxane) spacer. We show that the cavity of the system can be accurately tuned with either piezoelectric actuators or microflow-induced pressure to result in resonant coupling between a cavity mode and a variational mode of the solution molecules. Acrylonitrile solutions were chosen to prove the concept of vabriational strong coupling (VSC) of a CN stretching mode with light inside the cavity. We also show that the Rabi splitting energy is linearly proportional to the square root of molecular concentration, thereby proving the relevance and reliability of the system for VSC studies. |
Bioinorganic Chemistry: A Field Where Biomimetism and Bioinspiration Are Central Article de journal Clotilde Policar Inorganic Chemistry, 63 (50), p. 23475-23478, 2024, (PMID: 39676558). @article{doi:10.1021/acs.inorgchem.4c04868, title = {Bioinorganic Chemistry: A Field Where Biomimetism and Bioinspiration Are Central}, author = {Clotilde Policar}, url = {https://doi.org/10.1021/acs.inorgchem.4c04868}, doi = {10.1021/acs.inorgchem.4c04868}, year = {2024}, date = {2024-01-01}, journal = {Inorganic Chemistry}, volume = {63}, number = {50}, pages = {23475-23478}, note = {PMID: 39676558}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Cellular evaluation of superoxide dismutase mimics as catalytic drugs: Challenges and opportunities Article de journal Gabrielle Schanne; Sylvie Demignot; Clotilde Policar; Nicolas Delsuc Coordination Chemistry Reviews, 514 , p. 215906, 2024, ISSN: 0010-8545. @article{SCHANNE2024215906, title = {Cellular evaluation of superoxide dismutase mimics as catalytic drugs: Challenges and opportunities}, author = {Gabrielle Schanne and Sylvie Demignot and Clotilde Policar and Nicolas Delsuc}, url = {https://www.sciencedirect.com/science/article/pii/S0010854524002522}, doi = {https://doi.org/10.1016/j.ccr.2024.215906}, issn = {0010-8545}, year = {2024}, date = {2024-01-01}, journal = {Coordination Chemistry Reviews}, volume = {514}, pages = {215906}, abstract = {Oxidative stress is known to be associated with many pathologies including inflammation, cancer, diabetes, etc. However, oxidative stress resulting from the imbalance between reactive oxygen species flows and antioxidant defenses has been largely overlooked so far as a therapeutic target. Among antioxidant defenses, superoxide dismutases (SOD) are metalloenzymes that catalyze efficiently the dismutation of superoxide, the first reactive oxygen species resulting from the monoelectronic reduction of dioxygen. Superoxide, as a quite reactive chemical species, is a transient species. So, the cellular evaluation of metal complexes mimicking SOD (SOD mimics) in cellular models can be particularly tedious and calls for multiple direct and indirect strategies including probes and biochemical assays. This review highlights methods and assays to evaluate in cells SOD mimics, a new class of catalytic antioxidants.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Oxidative stress is known to be associated with many pathologies including inflammation, cancer, diabetes, etc. However, oxidative stress resulting from the imbalance between reactive oxygen species flows and antioxidant defenses has been largely overlooked so far as a therapeutic target. Among antioxidant defenses, superoxide dismutases (SOD) are metalloenzymes that catalyze efficiently the dismutation of superoxide, the first reactive oxygen species resulting from the monoelectronic reduction of dioxygen. Superoxide, as a quite reactive chemical species, is a transient species. So, the cellular evaluation of metal complexes mimicking SOD (SOD mimics) in cellular models can be particularly tedious and calls for multiple direct and indirect strategies including probes and biochemical assays. This review highlights methods and assays to evaluate in cells SOD mimics, a new class of catalytic antioxidants. |
Clotilde Policar; Nicolas Delsuc; Hél`ene Charlotte Bertrand Comptes Rendus. Chimie, 2024, (Online first). @article{CRCHIM_2024__27_S2_A12_0, title = {Metal complexes in cells: from design of catalytic antioxidants to imaging metal ions and designing metal-based probes in X-ray fluorescence and IR-imaging, a multidisciplinary collaborative journey in bioinorganic chemistry and inorganic chemical biology}, author = {Clotilde Policar and Nicolas Delsuc and H\'{e}l`ene Charlotte Bertrand}, doi = {10.5802/crchim.295}, year = {2024}, date = {2024-01-01}, journal = {Comptes Rendus. Chimie}, publisher = {Acad\'{e}mie des sciences, Paris}, note = {Online first}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Pt(iv) anticancer prodrugs bearing an oxaliplatin scaffold: what do we know about their bioactivity? Article de journal Alvaro Lopez-Sanchez; Helene C Bertrand Inorg. Chem. Front., 11 , p. 1639-1667, 2024. @article{D3QI02602G, title = {Pt(iv) anticancer prodrugs bearing an oxaliplatin scaffold: what do we know about their bioactivity?}, author = {Alvaro Lopez-Sanchez and Helene C Bertrand}, url = {http://dx.doi.org/10.1039/D3QI02602G}, doi = {10.1039/D3QI02602G}, year = {2024}, date = {2024-01-01}, journal = {Inorg. Chem. Front.}, volume = {11}, pages = {1639-1667}, publisher = {The Royal Society of Chemistry}, abstract = {Cancer remains a significant global health challenge, necessitating continuous advancements in therapeutic strategies. Chemotherapeutic agents have long been pivotal in cancer treatment, with platinum(Pt)-based drugs holding a prominent place. Oxaliplatin, a third-generation Pt(ii) compound, has gathered attention for its efficacy towards several cisplatin-resistant cancer cells and has become the front-line therapy for metastatic colorectal cancer. However, inherent limitations such as resistance development and dose-dependent side effects like oxaliplatin-induced peripheral neuropathy (OIPN) prompt the exploration of novel derivatives. Pt(iv) prodrugs have emerged as a promising avenue in cancer therapy, exploiting the intrinsic cytotoxicity of platinum while offering enhanced stability and tunable pharmacokinetics. However, the majority of Pt(iv) prodrugs reported in the literature, for their in vitro or in vivo anticancer properties, are cisplatin-based. This comprehensive review gathers, to our knowledge, the recent advances on oxaliplatin-based Pt(iv) derivatives and how they can strategically address the aforementioned challenges.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Cancer remains a significant global health challenge, necessitating continuous advancements in therapeutic strategies. Chemotherapeutic agents have long been pivotal in cancer treatment, with platinum(Pt)-based drugs holding a prominent place. Oxaliplatin, a third-generation Pt(ii) compound, has gathered attention for its efficacy towards several cisplatin-resistant cancer cells and has become the front-line therapy for metastatic colorectal cancer. However, inherent limitations such as resistance development and dose-dependent side effects like oxaliplatin-induced peripheral neuropathy (OIPN) prompt the exploration of novel derivatives. Pt(iv) prodrugs have emerged as a promising avenue in cancer therapy, exploiting the intrinsic cytotoxicity of platinum while offering enhanced stability and tunable pharmacokinetics. However, the majority of Pt(iv) prodrugs reported in the literature, for their in vitro or in vivo anticancer properties, are cisplatin-based. This comprehensive review gathers, to our knowledge, the recent advances on oxaliplatin-based Pt(iv) derivatives and how they can strategically address the aforementioned challenges. |
2023 |
Hydrogen Peroxide Signaling in Physiology and Pathology Article de journal Christine Rampon; Sophie Vriz Antioxidants, 12 (3), p. 661, 2023, ISSN: 2076-3921, (Number: 3 Publisher: Multidisciplinary Digital Publishing Institute). @article{rampon_hydrogen_2023, title = {Hydrogen Peroxide Signaling in Physiology and Pathology}, author = {Christine Rampon and Sophie Vriz}, url = {https://www.mdpi.com/2076-3921/12/3/661}, doi = {10.3390/antiox12030661}, issn = {2076-3921}, year = {2023}, date = {2023-01-01}, urldate = {2023-10-31}, journal = {Antioxidants}, volume = {12}, number = {3}, pages = {661}, abstract = {Reactive oxygen species (ROS) were originally described as toxic by-products of aerobic cellular energy metabolism associated with the development of several diseases, such as cancer, neurodegenerative diseases, and diabetes [...]}, note = {Number: 3 Publisher: Multidisciplinary Digital Publishing Institute}, keywords = {}, pubstate = {published}, tppubtype = {article} } Reactive oxygen species (ROS) were originally described as toxic by-products of aerobic cellular energy metabolism associated with the development of several diseases, such as cancer, neurodegenerative diseases, and diabetes [...] |
TMED10 mediates the loading of neosynthesised Sonic Hedgehog in COPII vesicles for efficient secretion and signalling Article de journal Yonis Bare; Tamás Matusek; Sophie Vriz; Maika S Deffieu; Pascal P Thérond; Raphael Gaudin Cellular and molecular life sciences: CMLS, 80 (9), p. 266, 2023, ISSN: 1420-9071. @article{bare_tmed10_2023, title = {TMED10 mediates the loading of neosynthesised Sonic Hedgehog in COPII vesicles for efficient secretion and signalling}, author = {Yonis Bare and Tam\'{a}s Matusek and Sophie Vriz and Maika S Deffieu and Pascal P Th\'{e}rond and Raphael Gaudin}, doi = {10.1007/s00018-023-04918-1}, issn = {1420-9071}, year = {2023}, date = {2023-01-01}, journal = {Cellular and molecular life sciences: CMLS}, volume = {80}, number = {9}, pages = {266}, abstract = {The morphogen Sonic Hedgehog (SHH) plays an important role in coordinating embryonic development. Short- and long-range SHH signalling occurs through a variety of membrane-associated and membrane-free forms. However, the molecular mechanisms that govern the early events of the trafficking of neosynthesised SHH in mammalian cells are still poorly understood. Here, we employed the retention using selective hooks (RUSH) system to show that newly-synthesised SHH is trafficked through the classical biosynthetic secretory pathway, using TMED10 as an endoplasmic reticulum (ER) cargo receptor for efficient ER-to-Golgi transport and Rab6 vesicles for Golgi-to-cell surface trafficking. TMED10 and SHH colocalized at ER exit sites (ERES), and TMED10 depletion significantly delays SHH loading onto ERES and subsequent exit leading to significant SHH release defects. Finally, we utilised the Drosophila wing imaginal disc model to demonstrate that the homologue of TMED10, Baiser (Bai), participates in Hedgehog (Hh) secretion and signalling in vivo. In conclusion, our work highlights the role of TMED10 in cargo-specific egress from the ER and sheds light on novel important partners of neosynthesised SHH secretion with potential impact on embryonic development.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The morphogen Sonic Hedgehog (SHH) plays an important role in coordinating embryonic development. Short- and long-range SHH signalling occurs through a variety of membrane-associated and membrane-free forms. However, the molecular mechanisms that govern the early events of the trafficking of neosynthesised SHH in mammalian cells are still poorly understood. Here, we employed the retention using selective hooks (RUSH) system to show that newly-synthesised SHH is trafficked through the classical biosynthetic secretory pathway, using TMED10 as an endoplasmic reticulum (ER) cargo receptor for efficient ER-to-Golgi transport and Rab6 vesicles for Golgi-to-cell surface trafficking. TMED10 and SHH colocalized at ER exit sites (ERES), and TMED10 depletion significantly delays SHH loading onto ERES and subsequent exit leading to significant SHH release defects. Finally, we utilised the Drosophila wing imaginal disc model to demonstrate that the homologue of TMED10, Baiser (Bai), participates in Hedgehog (Hh) secretion and signalling in vivo. In conclusion, our work highlights the role of TMED10 in cargo-specific egress from the ER and sheds light on novel important partners of neosynthesised SHH secretion with potential impact on embryonic development. |
Ruthenium(II) polypyridyl complexes as emerging photosensitisers for antibacterial photodynamic therapy Article de journal Ng Xiao Ying; Fong Kar Wai; Kiew Lik Voon; Katrina Chung Pooi Yin; Liew Yun Khoon; Nicolas Delsuc; Mohd Zulkefeli; Low May Lee Journal of Inorganic Biochemistry, p. 112425, 2023, ISSN: 0162-0134. @article{YING2023112425, title = {Ruthenium(II) polypyridyl complexes as emerging photosensitisers for antibacterial photodynamic therapy}, author = {Ng Xiao Ying and Fong Kar Wai and Kiew Lik Voon and Katrina Chung Pooi Yin and Liew Yun Khoon and Nicolas Delsuc and Mohd Zulkefeli and Low May Lee}, url = {https://www.sciencedirect.com/science/article/pii/S0162013423003070}, doi = {https://doi.org/10.1016/j.jinorgbio.2023.112425}, issn = {0162-0134}, year = {2023}, date = {2023-01-01}, journal = {Journal of Inorganic Biochemistry}, pages = {112425}, abstract = {Photodynamic therapy (PDT) has recently emerged as a potential valuable alternative to treat microbial infections. In PDT, singlet oxygen is generated in the presence of photosensitisers and oxygen under light irradiation of a specific wavelength, causing cytotoxic damage to bacteria. This review highlights different generations of photosensitisers and the common characteristics of ideal photosensitisers. It also focuses on the emergence of ruthenium and more specifically on Ru(II) polypyridyl complexes as metal-based photosensitisers used in antimicrobial photodynamic therapy (aPDT). Their photochemical and photophysical properties as well as structures are discussed while relating them to their phototoxicity. The use of Ru(II) complexes with recent advancements such as nanoformulations, combinatory therapy and photothermal therapy to improve on previous shortcomings of the complexes are outlined. Future perspectives of these complexes used in two-photon PDT, photoacoustic imaging and sonotherapy are also discussed. This review covers the literature published from 2017 to 2023.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Photodynamic therapy (PDT) has recently emerged as a potential valuable alternative to treat microbial infections. In PDT, singlet oxygen is generated in the presence of photosensitisers and oxygen under light irradiation of a specific wavelength, causing cytotoxic damage to bacteria. This review highlights different generations of photosensitisers and the common characteristics of ideal photosensitisers. It also focuses on the emergence of ruthenium and more specifically on Ru(II) polypyridyl complexes as metal-based photosensitisers used in antimicrobial photodynamic therapy (aPDT). Their photochemical and photophysical properties as well as structures are discussed while relating them to their phototoxicity. The use of Ru(II) complexes with recent advancements such as nanoformulations, combinatory therapy and photothermal therapy to improve on previous shortcomings of the complexes are outlined. Future perspectives of these complexes used in two-photon PDT, photoacoustic imaging and sonotherapy are also discussed. This review covers the literature published from 2017 to 2023. |
2022 |
Assessment of iron nanoparticle distribution in mouse models using ultrashort-echo-time MRI Article de journal Andreas Boss; Laura Heeb; Divya Vats; Fabian H L Starsich; Alice Balfourier; Inge K Herrmann; Anurag Gupta NMR in Biomedicine, p. e4690, 2022, ISSN: 1099-1492. @article{boss_assessment_nodate, title = {Assessment of iron nanoparticle distribution in mouse models using ultrashort-echo-time MRI}, author = {Andreas Boss and Laura Heeb and Divya Vats and Fabian H L Starsich and Alice Balfourier and Inge K Herrmann and Anurag Gupta}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/nbm.4690}, doi = {10.1002/nbm.4690}, issn = {1099-1492}, year = {2022}, date = {2022-01-01}, urldate = {2022-04-21}, journal = {NMR in Biomedicine}, pages = {e4690}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
An early Shh-H2O2 reciprocal regulatory interaction controls the regenerative program during zebrafish fin regeneration Article de journal Marion Thauvin; Rodolphe Matias de Sousa; Marine Alves; Michel Volovitch; Sophie Vriz; Christine Rampon Journal of Cell Science, 135 (6), p. jcs259664, 2022, ISSN: 1477-9137. @article{thauvin_early_2022, title = {An early Shh-H2O2 reciprocal regulatory interaction controls the regenerative program during zebrafish fin regeneration}, author = {Marion Thauvin and Rodolphe Matias de Sousa and Marine Alves and Michel Volovitch and Sophie Vriz and Christine Rampon}, doi = {10.1242/jcs.259664}, issn = {1477-9137}, year = {2022}, date = {2022-01-01}, journal = {Journal of Cell Science}, volume = {135}, number = {6}, pages = {jcs259664}, abstract = {Reactive oxygen species (ROS), originally classified as toxic molecules, have attracted increasing interest given their actions in cell signaling. Hydrogen peroxide (H2O2), the major ROS produced by cells, acts as a second messenger to modify redox-sensitive proteins or lipids. After caudal fin amputation, tight spatiotemporal regulation of ROS is required first for wound healing and later to initiate the regenerative program. However, the mechanisms carrying out this sustained ROS production and their integration with signaling pathways remain poorly understood. We focused on the early dialog between H2O2 and Sonic hedgehog (Shh) during zebrafish fin regeneration. We demonstrate that H2O2 controls Shh expression and that Shh in turn regulates the H2O2 level via a canonical pathway. Moreover, the means of this tight reciprocal control change during the successive phases of the regenerative program. Dysregulation of the Hedgehog pathway has been implicated in several developmental syndromes, diabetes and cancer. These data support the existence of an early positive crosstalk between Shh and H2O2 that might be more generally involved in various processes paving the way to improve regenerative processes, particularly in vertebrates.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Reactive oxygen species (ROS), originally classified as toxic molecules, have attracted increasing interest given their actions in cell signaling. Hydrogen peroxide (H2O2), the major ROS produced by cells, acts as a second messenger to modify redox-sensitive proteins or lipids. After caudal fin amputation, tight spatiotemporal regulation of ROS is required first for wound healing and later to initiate the regenerative program. However, the mechanisms carrying out this sustained ROS production and their integration with signaling pathways remain poorly understood. We focused on the early dialog between H2O2 and Sonic hedgehog (Shh) during zebrafish fin regeneration. We demonstrate that H2O2 controls Shh expression and that Shh in turn regulates the H2O2 level via a canonical pathway. Moreover, the means of this tight reciprocal control change during the successive phases of the regenerative program. Dysregulation of the Hedgehog pathway has been implicated in several developmental syndromes, diabetes and cancer. These data support the existence of an early positive crosstalk between Shh and H2O2 that might be more generally involved in various processes paving the way to improve regenerative processes, particularly in vertebrates. |
NADPH-Oxidase Derived Hydrogen Peroxide and Irs2b Facilitate Re-oxygenation-Induced Catch-Up Growth in Zebrafish Embryo Article de journal Ayaka Zasu; Futa Hishima; Marion Thauvin; Yosuke Yoneyama; Yoichiro Kitani; Fumihiko Hakuno; Michel Volovitch; Shin-Ichiro Takahashi; Sophie Vriz; Christine Rampon; Hiroyasu Kamei Frontiers in Endocrinology, 13 , 2022, ISSN: 1664-2392. @article{zasu_nadph-oxidase_2022, title = {NADPH-Oxidase Derived Hydrogen Peroxide and Irs2b Facilitate Re-oxygenation-Induced Catch-Up Growth in Zebrafish Embryo}, author = {Ayaka Zasu and Futa Hishima and Marion Thauvin and Yosuke Yoneyama and Yoichiro Kitani and Fumihiko Hakuno and Michel Volovitch and Shin-Ichiro Takahashi and Sophie Vriz and Christine Rampon and Hiroyasu Kamei}, url = {https://www.frontiersin.org/articles/10.3389/fendo.2022.929668}, issn = {1664-2392}, year = {2022}, date = {2022-01-01}, urldate = {2023-10-31}, journal = {Frontiers in Endocrinology}, volume = {13}, abstract = {Oxygen deprivation induces multiple changes at the cellular and organismal levels, and its re-supply also brings another special physiological status. We have investigated the effects of hypoxia/re-oxygenation on embryonic growth using the zebrafish model: hypoxia slows embryonic growth, but re-oxygenation induces growth spurt or catch-up growth. The mitogen-activated kinase (MAPK)-pathway downstream insulin-like growth factor (IGF/Igf) has been revealed to positively regulate the re-oxygenation-induced catch-up growth, and the role of reactive oxygen species generated by environmental oxygen fluctuation is potentially involved in the phenomenon. Here, we report the role of NADPH-oxidase (Nox)-dependent hydrogen peroxide (H2O2) production in the MAPK-activation and catch-up growth. The inhibition of Nox significantly blunted catch-up growth and MAPK-activity. Amongst two zebrafish insulin receptor substrate 2 genes (irs2a and irs2b), the loss of irs2b, but not its paralog irs2a, resulted in blunted MAPK-activation and catch-up growth. Furthermore, irs2b forcedly expressed in mammalian cells allowed IGF-MAPK augmentation in the presence of H2O2, and the irs2b deficiency completely abolished the somatotropic action of Nox in re-oxygenation condition. These results indicate that redox signaling alters IGF/Igf signaling to facilitate hypoxia/re-oxygenation-induced embryonic growth compensation.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Oxygen deprivation induces multiple changes at the cellular and organismal levels, and its re-supply also brings another special physiological status. We have investigated the effects of hypoxia/re-oxygenation on embryonic growth using the zebrafish model: hypoxia slows embryonic growth, but re-oxygenation induces growth spurt or catch-up growth. The mitogen-activated kinase (MAPK)-pathway downstream insulin-like growth factor (IGF/Igf) has been revealed to positively regulate the re-oxygenation-induced catch-up growth, and the role of reactive oxygen species generated by environmental oxygen fluctuation is potentially involved in the phenomenon. Here, we report the role of NADPH-oxidase (Nox)-dependent hydrogen peroxide (H2O2) production in the MAPK-activation and catch-up growth. The inhibition of Nox significantly blunted catch-up growth and MAPK-activity. Amongst two zebrafish insulin receptor substrate 2 genes (irs2a and irs2b), the loss of irs2b, but not its paralog irs2a, resulted in blunted MAPK-activation and catch-up growth. Furthermore, irs2b forcedly expressed in mammalian cells allowed IGF-MAPK augmentation in the presence of H2O2, and the irs2b deficiency completely abolished the somatotropic action of Nox in re-oxygenation condition. These results indicate that redox signaling alters IGF/Igf signaling to facilitate hypoxia/re-oxygenation-induced embryonic growth compensation. |
Reciprocal Regulation of Shh Trafficking and H2O2 Levels via a Noncanonical BOC-Rac1 Pathway Article de journal Marion Thauvin; Irène Amblard; Christine Rampon; Aurélien Mourton; Isabelle Queguiner; Chenge Li; Arnaud Gautier; Alain Joliot; Michel Volovitch; Sophie Vriz Antioxidants (Basel, Switzerland), 11 (4), p. 718, 2022, ISSN: 2076-3921. @article{thauvin_reciprocal_2022, title = {Reciprocal Regulation of Shh Trafficking and H2O2 Levels via a Noncanonical BOC-Rac1 Pathway}, author = {Marion Thauvin and Ir\`{e}ne Amblard and Christine Rampon and Aur\'{e}lien Mourton and Isabelle Queguiner and Chenge Li and Arnaud Gautier and Alain Joliot and Michel Volovitch and Sophie Vriz}, doi = {10.3390/antiox11040718}, issn = {2076-3921}, year = {2022}, date = {2022-01-01}, journal = {Antioxidants (Basel, Switzerland)}, volume = {11}, number = {4}, pages = {718}, abstract = {Among molecules that bridge environment, cell metabolism, and cell signaling, hydrogen peroxide (H2O2) recently appeared as an emerging but central player. Its level depends on cell metabolism and environment and was recently shown to play key roles during embryogenesis, contrasting with its long-established role in disease progression. We decided to explore whether the secreted morphogen Sonic hedgehog (Shh), known to be essential in a variety of biological processes ranging from embryonic development to adult tissue homeostasis and cancers, was part of these interactions. Here, we report that H2O2 levels control key steps of Shh delivery in cell culture: increased levels reduce primary secretion, stimulate endocytosis and accelerate delivery to recipient cells; in addition, physiological in vivo modulation of H2O2 levels changes Shh distribution and tissue patterning. Moreover, a feedback loop exists in which Shh trafficking controls H2O2 synthesis via a non-canonical BOC-Rac1 pathway, leading to cytoneme growth. Our findings reveal that Shh directly impacts its own distribution, thus providing a molecular explanation for the robustness of morphogenesis to both environmental insults and individual variability.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Among molecules that bridge environment, cell metabolism, and cell signaling, hydrogen peroxide (H2O2) recently appeared as an emerging but central player. Its level depends on cell metabolism and environment and was recently shown to play key roles during embryogenesis, contrasting with its long-established role in disease progression. We decided to explore whether the secreted morphogen Sonic hedgehog (Shh), known to be essential in a variety of biological processes ranging from embryonic development to adult tissue homeostasis and cancers, was part of these interactions. Here, we report that H2O2 levels control key steps of Shh delivery in cell culture: increased levels reduce primary secretion, stimulate endocytosis and accelerate delivery to recipient cells; in addition, physiological in vivo modulation of H2O2 levels changes Shh distribution and tissue patterning. Moreover, a feedback loop exists in which Shh trafficking controls H2O2 synthesis via a non-canonical BOC-Rac1 pathway, leading to cytoneme growth. Our findings reveal that Shh directly impacts its own distribution, thus providing a molecular explanation for the robustness of morphogenesis to both environmental insults and individual variability. |
Fgf8 dynamics and critical slowing down may account for the temperature independence of somitogenesis Article de journal Weiting Zhang; Pierluigi Scerbo; Marine Delagrange; Virginie Candat; Vanessa Mayr; Sophie Vriz; Martin Distel; Bertrand Ducos; David Bensimon Communications Biology, 5 (1), p. 1–10, 2022, ISSN: 2399-3642, (Number: 1 Publisher: Nature Publishing Group). @article{zhang_fgf8_2022, title = {Fgf8 dynamics and critical slowing down may account for the temperature independence of somitogenesis}, author = {Weiting Zhang and Pierluigi Scerbo and Marine Delagrange and Virginie Candat and Vanessa Mayr and Sophie Vriz and Martin Distel and Bertrand Ducos and David Bensimon}, url = {https://www.nature.com/articles/s42003-022-03053-0}, doi = {10.1038/s42003-022-03053-0}, issn = {2399-3642}, year = {2022}, date = {2022-01-01}, urldate = {2023-10-31}, journal = {Communications Biology}, volume = {5}, number = {1}, pages = {1--10}, abstract = {Somitogenesis, the segmentation of the antero-posterior axis in vertebrates, is thought to result from the interactions between a genetic oscillator and a posterior-moving determination wavefront. The segment (somite) size is set by the product of the oscillator period and the velocity of the determination wavefront. Surprisingly, while the segmentation period can vary by a factor three between 20 °C and 32 °C, the somite size is constant. How this temperature independence is achieved is a mystery that we address in this study. Using RT-qPCR we show that the endogenous fgf8 mRNA concentration decreases during somitogenesis and correlates with the exponent of the shrinking pre-somitic mesoderm (PSM) size. As the temperature decreases, the dynamics of fgf8 and many other gene transcripts, as well as the segmentation frequency and the PSM shortening and tail growth rates slows down as T\textendashTc (with Tc = 14.4 °C). This behavior characteristic of a system near a critical point may account for the temperature independence of somitogenesis in zebrafish.}, note = {Number: 1 Publisher: Nature Publishing Group}, keywords = {}, pubstate = {published}, tppubtype = {article} } Somitogenesis, the segmentation of the antero-posterior axis in vertebrates, is thought to result from the interactions between a genetic oscillator and a posterior-moving determination wavefront. The segment (somite) size is set by the product of the oscillator period and the velocity of the determination wavefront. Surprisingly, while the segmentation period can vary by a factor three between 20 °C and 32 °C, the somite size is constant. How this temperature independence is achieved is a mystery that we address in this study. Using RT-qPCR we show that the endogenous fgf8 mRNA concentration decreases during somitogenesis and correlates with the exponent of the shrinking pre-somitic mesoderm (PSM) size. As the temperature decreases, the dynamics of fgf8 and many other gene transcripts, as well as the segmentation frequency and the PSM shortening and tail growth rates slows down as T–Tc (with Tc = 14.4 °C). This behavior characteristic of a system near a critical point may account for the temperature independence of somitogenesis in zebrafish. |
Hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives Article de journal Alexander I Kostyuk; Maria-Armineh Tossounian; Anastasiya S Panova; Marion Thauvin; Roman I Raevskii; Daria Ezeriņa; Khadija Wahni; Inge Van Molle; Anastasia D Sergeeva; Didier Vertommen; Andrey Yu. Gorokhovatsky; Mikhail S Baranov; Sophie Vriz; Joris Messens; Dmitry S Bilan; Vsevolod V Belousov Nature Communications, 13 , p. 171, 2022, ISSN: 2041-1723. @article{kostyuk_hypocrates_2022, title = {Hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives}, author = {Alexander I Kostyuk and Maria-Armineh Tossounian and Anastasiya S Panova and Marion Thauvin and Roman I Raevskii and Daria Ezeri\c{n}a and Khadija Wahni and Inge Van Molle and Anastasia D Sergeeva and Didier Vertommen and Andrey Yu. Gorokhovatsky and Mikhail S Baranov and Sophie Vriz and Joris Messens and Dmitry S Bilan and Vsevolod V Belousov}, url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8748444/}, doi = {10.1038/s41467-021-27796-2}, issn = {2041-1723}, year = {2022}, date = {2022-01-01}, urldate = {2023-10-31}, journal = {Nature Communications}, volume = {13}, pages = {171}, abstract = {The lack of tools to monitor the dynamics of (pseudo)hypohalous acids in live cells and tissues hinders a better understanding of inflammatory processes. Here we present a fluorescent genetically encoded biosensor, Hypocrates, for the visualization of (pseudo)hypohalous acids and their derivatives. Hypocrates consists of a circularly permuted yellow fluorescent protein integrated into the structure of the transcription repressor NemR from Escherichia coli. We show that Hypocrates is ratiometric, reversible, and responds to its analytes in the 106 M−1s−1 range. Solving the Hypocrates X-ray structure provided insights into its sensing mechanism, allowing determination of the spatial organization in this circularly permuted fluorescent protein-based redox probe. We exemplify its applicability by imaging hypohalous stress in bacteria phagocytosed by primary neutrophils. Finally, we demonstrate that Hypocrates can be utilized in combination with HyPerRed for the simultaneous visualization of (pseudo)hypohalous acids and hydrogen peroxide dynamics in a zebrafish tail fin injury model., There are a lack of tools to study the dynamics of (pseudo)hypohalous acids in live cells. Here the authors report a genetically encoded fluorescent biosensor, Hypocrates, for (pseudo)hypohalous acids and their derivatives which they use in cells and in a zebrafish tail fin injury model.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The lack of tools to monitor the dynamics of (pseudo)hypohalous acids in live cells and tissues hinders a better understanding of inflammatory processes. Here we present a fluorescent genetically encoded biosensor, Hypocrates, for the visualization of (pseudo)hypohalous acids and their derivatives. Hypocrates consists of a circularly permuted yellow fluorescent protein integrated into the structure of the transcription repressor NemR from Escherichia coli. We show that Hypocrates is ratiometric, reversible, and responds to its analytes in the 106 M−1s−1 range. Solving the Hypocrates X-ray structure provided insights into its sensing mechanism, allowing determination of the spatial organization in this circularly permuted fluorescent protein-based redox probe. We exemplify its applicability by imaging hypohalous stress in bacteria phagocytosed by primary neutrophils. Finally, we demonstrate that Hypocrates can be utilized in combination with HyPerRed for the simultaneous visualization of (pseudo)hypohalous acids and hydrogen peroxide dynamics in a zebrafish tail fin injury model., There are a lack of tools to study the dynamics of (pseudo)hypohalous acids in live cells. Here the authors report a genetically encoded fluorescent biosensor, Hypocrates, for (pseudo)hypohalous acids and their derivatives which they use in cells and in a zebrafish tail fin injury model. |
SOD mimics: From the tool box of the chemists to cellular studies Article de journal Clotilde Policar; Jean Bouvet; Hélène C Bertrand; Nicolas Delsuc Current Opinion in Chemical Biology, 67 , p. 102109, 2022, ISSN: 1367-5931. @article{POLICAR2022102109, title = {SOD mimics: From the tool box of the chemists to cellular studies}, author = {Clotilde Policar and Jean Bouvet and H\'{e}l\`{e}ne C Bertrand and Nicolas Delsuc}, url = {https://www.sciencedirect.com/science/article/pii/S136759312100154X}, doi = {https://doi.org/10.1016/j.cbpa.2021.102109}, issn = {1367-5931}, year = {2022}, date = {2022-01-01}, journal = {Current Opinion in Chemical Biology}, volume = {67}, pages = {102109}, abstract = {Superoxide dismutases (SODs) are metalloproteins that protect cells against oxidative stress by controlling the concentration of superoxide (O2−) through catalysis of its dismutation. The activity of superoxide dismutases can be mimicked by low-molecular-weight complexes having potential therapeutic applications. This review presents recent strategies for designing efficient SOD mimics, from molecular metal complexes to nanomaterials. Studies of these systems in cells reveal that some SOD mimics, designed to react directly with superoxide, may also indirectly enhance the cellular antioxidant arsenal. Finally, a good understanding of the bioactivity requires information on the cell-penetration, speciation, and subcellular location of the SOD mimics: we will describe recent studies and new techniques that open opportunities for characterizing SOD mimics in biological environments.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Superoxide dismutases (SODs) are metalloproteins that protect cells against oxidative stress by controlling the concentration of superoxide (O2−) through catalysis of its dismutation. The activity of superoxide dismutases can be mimicked by low-molecular-weight complexes having potential therapeutic applications. This review presents recent strategies for designing efficient SOD mimics, from molecular metal complexes to nanomaterials. Studies of these systems in cells reveal that some SOD mimics, designed to react directly with superoxide, may also indirectly enhance the cellular antioxidant arsenal. Finally, a good understanding of the bioactivity requires information on the cell-penetration, speciation, and subcellular location of the SOD mimics: we will describe recent studies and new techniques that open opportunities for characterizing SOD mimics in biological environments. |
Cellular Detection of a Mitochondria Targeted Brominated Vinyl Triphenylamine Optical Probe (TP−Br) by X-Ray Fluorescence Microscopy Article de journal Sounderya Nagarajan; Florent Poyer; Laura Fourmois; Delphine Naud-Martin; Kadda Medjoubi; Andrea Somogyi; Gabrielle Schanne; Lucas Henry; Nicolas Delsuc; Clotilde Policar; Helene C Bertrand; Florence Mahuteau-Betzer Chemistry – A European Journal, 28 (15), p. e202104424, 2022. @article{https://doi.org/10.1002/chem.202104424, title = {Cellular Detection of a Mitochondria Targeted Brominated Vinyl Triphenylamine Optical Probe (TP−Br) by X-Ray Fluorescence Microscopy}, author = {Sounderya Nagarajan and Florent Poyer and Laura Fourmois and Delphine Naud-Martin and Kadda Medjoubi and Andrea Somogyi and Gabrielle Schanne and Lucas Henry and Nicolas Delsuc and Clotilde Policar and Helene C Bertrand and Florence Mahuteau-Betzer}, url = {https://chemistry-europe.onlinelibrary.wiley.com/doi/abs/10.1002/chem.202104424}, doi = {https://doi.org/10.1002/chem.202104424}, year = {2022}, date = {2022-01-01}, journal = {Chemistry \textendash A European Journal}, volume = {28}, number = {15}, pages = {e202104424}, abstract = {Abstract Triphenylamine (TP) derivatives such as two-branch cationic vinylbenzimidazolium triphenylamine TP−2Bzim are promising turn-on fluorescent probes suitable for two-photon imaging, labelling mitochondria in live cells. Here, we designed two TP−2Bzim derivatives as bimodal probes suitable for X-ray fluorescence imaging. The conjugation of the TP core with a rhenium tricarbonyl moiety in the TP−RePyta probe altered the localisation in live cells from mitochondria to lysosomes. The introduction of bromine on the TP core generated the TP−Br probe retaining good photophysical properties and mitochondria labelling in live cells. The influence of calcium channels in the uptake of TP−Br was studied. Synchrotron Radiation X-ray Fluorescence (SXRF) imaging of bromine enabled the detection of TP−Br and suggested a negligible presence of the probe in an unbound state in the incubated cells, a crucial point in the development of these probes. This study paves the way towards the development of TP probes as specific organelle stainers suitable for SXRF imaging.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Triphenylamine (TP) derivatives such as two-branch cationic vinylbenzimidazolium triphenylamine TP−2Bzim are promising turn-on fluorescent probes suitable for two-photon imaging, labelling mitochondria in live cells. Here, we designed two TP−2Bzim derivatives as bimodal probes suitable for X-ray fluorescence imaging. The conjugation of the TP core with a rhenium tricarbonyl moiety in the TP−RePyta probe altered the localisation in live cells from mitochondria to lysosomes. The introduction of bromine on the TP core generated the TP−Br probe retaining good photophysical properties and mitochondria labelling in live cells. The influence of calcium channels in the uptake of TP−Br was studied. Synchrotron Radiation X-ray Fluorescence (SXRF) imaging of bromine enabled the detection of TP−Br and suggested a negligible presence of the probe in an unbound state in the incubated cells, a crucial point in the development of these probes. This study paves the way towards the development of TP probes as specific organelle stainers suitable for SXRF imaging. |
Inertness of Superoxide Dismutase Mimics Mn(II) Complexes Based on an Open-Chain Ligand, Bioactivity, and Detection in Intestinal Epithelial Cells Article de journal Gabrielle Schanne; Martha Zoumpoulaki; Géraldine Gazzah; Amandine Vincent; Hugues Preud’homme; Ryszard Lobinski; Sylvie Demignot; Philippe Seksik; Nicolas Delsuc; Clotilde Policar Oxidative Medicine and Cellular Longevity, 2022 , p. e3858122, 2022, ISSN: 1942-0900, (Publisher: Hindawi). @article{schanne_inertness_2022, title = {Inertness of Superoxide Dismutase Mimics Mn(II) Complexes Based on an Open-Chain Ligand, Bioactivity, and Detection in Intestinal Epithelial Cells}, author = {Gabrielle Schanne and Martha Zoumpoulaki and G\'{e}raldine Gazzah and Amandine Vincent and Hugues Preud’homme and Ryszard Lobinski and Sylvie Demignot and Philippe Seksik and Nicolas Delsuc and Clotilde Policar}, url = {https://www.hindawi.com/journals/omcl/2022/3858122/}, doi = {10.1155/2022/3858122}, issn = {1942-0900}, year = {2022}, date = {2022-01-01}, urldate = {2022-04-03}, journal = {Oxidative Medicine and Cellular Longevity}, volume = {2022}, pages = {e3858122}, abstract = {Oxidative stress is known to play a major role in the pathogenesis of inflammatory bowel diseases (IBDs), and, in particular, superoxide dismutase (SODs) defenses were shown to be weakened in patients suffering from IBDs. SOD mimics, also called SOD mimetics, as low-molecular-weight complexes reproducing the activity of SOD, constitute promising antioxidant catalytic metallodrugs in the context of IBDs. A Mn(II) complex SOD mimic (Mn1) based on an open-chain diaminoethane ligand exerting antioxidant and anti-inflammatory effects on an intestinal epithelial cellular model was shown to experience metal exchanges between the manganese center and metal ions present in the biological environment (such as Zn(II)) to some degrees. As the resulting complexes (mainly Zn(II)) were shown to be inactive, improving the kinetic inertness of Mn(II) complexes based on open-chain ligands is key to improve their bioactivity in a cellular context. We report here the study of three new Mn(II) complexes resulting from Mn1 functionalization with a cyclohexyl and/or a propyl group meant to limit, respectively, (a) metal exchanges and (b) deprotonation of an amine from the 1,2-diaminoethane central scaffold. The new manganese-based SOD mimics display a higher intrinsic SOD activity and also improved kinetic inertness in metal ion exchange processes (with Zn(II), Cu(II), Ni(II), and Co(II)). They were shown to provide anti-inflammatory and antioxidant effects in cells at lower doses than Mn1 (down to 10 μM). This improvement was due to their higher inertness against metal-assisted dissociation and not to different cellular overall accumulations. Based on its higher inertness, the SOD mimic containing both the propyl and the cyclohexyl moieties was suitable for intracellular detection and quantification by mass spectrometry, quantification, that was achieved by using a 13C-labeled Co-based analog of the SOD mimics as an external heavy standard.}, note = {Publisher: Hindawi}, keywords = {}, pubstate = {published}, tppubtype = {article} } Oxidative stress is known to play a major role in the pathogenesis of inflammatory bowel diseases (IBDs), and, in particular, superoxide dismutase (SODs) defenses were shown to be weakened in patients suffering from IBDs. SOD mimics, also called SOD mimetics, as low-molecular-weight complexes reproducing the activity of SOD, constitute promising antioxidant catalytic metallodrugs in the context of IBDs. A Mn(II) complex SOD mimic (Mn1) based on an open-chain diaminoethane ligand exerting antioxidant and anti-inflammatory effects on an intestinal epithelial cellular model was shown to experience metal exchanges between the manganese center and metal ions present in the biological environment (such as Zn(II)) to some degrees. As the resulting complexes (mainly Zn(II)) were shown to be inactive, improving the kinetic inertness of Mn(II) complexes based on open-chain ligands is key to improve their bioactivity in a cellular context. We report here the study of three new Mn(II) complexes resulting from Mn1 functionalization with a cyclohexyl and/or a propyl group meant to limit, respectively, (a) metal exchanges and (b) deprotonation of an amine from the 1,2-diaminoethane central scaffold. The new manganese-based SOD mimics display a higher intrinsic SOD activity and also improved kinetic inertness in metal ion exchange processes (with Zn(II), Cu(II), Ni(II), and Co(II)). They were shown to provide anti-inflammatory and antioxidant effects in cells at lower doses than Mn1 (down to 10 μM). This improvement was due to their higher inertness against metal-assisted dissociation and not to different cellular overall accumulations. Based on its higher inertness, the SOD mimic containing both the propyl and the cyclohexyl moieties was suitable for intracellular detection and quantification by mass spectrometry, quantification, that was achieved by using a 13C-labeled Co-based analog of the SOD mimics as an external heavy standard. |
Deciphering the Metal Speciation in Low-Molecular-Weight Complexes by IMS-MS: Application to the Detection of Manganese Superoxide Dismutase Mimics in Cell Lysates Article de journal Martha Zoumpoulaki; Gabrielle Schanne; Nicolas Delsuc; Hugues Preud'homme; Elodie Quévrain; Nicolas Eskenazi; Géraldine Gazzah; Regis Guillot; Philippe Seksik; Joelle Vinh; Ryszard Lobinski; Clotilde Policar Angewandte Chemie International Edition, n/a (n/a), p. e202203066, 2022. @article{https://doi.org/10.1002/anie.202203066, title = {Deciphering the Metal Speciation in Low-Molecular-Weight Complexes by IMS-MS: Application to the Detection of Manganese Superoxide Dismutase Mimics in Cell Lysates}, author = {Martha Zoumpoulaki and Gabrielle Schanne and Nicolas Delsuc and Hugues Preud'homme and Elodie Qu\'{e}vrain and Nicolas Eskenazi and G\'{e}raldine Gazzah and Regis Guillot and Philippe Seksik and Joelle Vinh and Ryszard Lobinski and Clotilde Policar}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.202203066}, doi = {https://doi.org/10.1002/anie.202203066}, year = {2022}, date = {2022-01-01}, journal = {Angewandte Chemie International Edition}, volume = {n/a}, number = {n/a}, pages = {e202203066}, abstract = {Abstract The detection and quantification of exogenous metal complexes are crucial to understanding their activity in intricate biological media. MnII complexes are difficult to detect and quantify because of low association constants, and high lability. The superoxide dismutase (SOD) mimic (or mimetic) labelled Mn1 is based on a 1,2-di-aminoethane functionalized with imidazole and phenolate and has good intrinsic anti-superoxide, antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-activated intestinal epithelial HT29-MD2 cells, similar to that of its propylated analogue labelled Mn1P. Ion mobility spectrometry-mass spectrometry (IMS-MS) is a powerful technique for separating low molecular weight (LMW) metal complexes and can even separate complexes with the same ligand but bound to different divalent metal cations with similar ionic radii. We demonstrated the intracellular presence of the Mn1 and Mn1P complexes, at least partly intact, in lysates of cells incubated with the complexes and estimated the intracellular Mn1P concentration using a Co-13C6 analogue.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract The detection and quantification of exogenous metal complexes are crucial to understanding their activity in intricate biological media. MnII complexes are difficult to detect and quantify because of low association constants, and high lability. The superoxide dismutase (SOD) mimic (or mimetic) labelled Mn1 is based on a 1,2-di-aminoethane functionalized with imidazole and phenolate and has good intrinsic anti-superoxide, antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-activated intestinal epithelial HT29-MD2 cells, similar to that of its propylated analogue labelled Mn1P. Ion mobility spectrometry-mass spectrometry (IMS-MS) is a powerful technique for separating low molecular weight (LMW) metal complexes and can even separate complexes with the same ligand but bound to different divalent metal cations with similar ionic radii. We demonstrated the intracellular presence of the Mn1 and Mn1P complexes, at least partly intact, in lysates of cells incubated with the complexes and estimated the intracellular Mn1P concentration using a Co-13C6 analogue. |
Importance of Metal Biotransformation in Cell Response to Metallic Nanoparticles: A Transcriptomic Meta-analysis Study Article de journal Alice Balfourier; Anne-Pia Marty; Florence Gazeau ACS Nanoscience Au, p. acsnanoscienceau.2c00035, 2022, ISSN: 2694-2496, 2694-2496. @article{balfourier_importance_2022, title = {Importance of Metal Biotransformation in Cell Response to Metallic Nanoparticles: A Transcriptomic Meta-analysis Study}, author = {Alice Balfourier and Anne-Pia Marty and Florence Gazeau}, doi = {10.1021/acsnanoscienceau.2c00035}, issn = {2694-2496, 2694-2496}, year = {2022}, date = {2022-01-01}, urldate = {2023-01-06}, journal = {ACS Nanoscience Au}, pages = {acsnanoscienceau.2c00035}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Alice Balfourier; Elena Tsolaki; Laura Heeb; Fabian HL Starsich; Daniel Klose; Andreas Boss; Anurag Gupta; Alexander Gogos; Inge K Herrmann Small Methods, p. 2201061, 2022. @article{balfourier2022multiscale, title = {Multiscale Multimodal Investigation of the Intratissural Biodistribution of Iron Nanotherapeutics with Single Cell Resolution Reveals Co-Localization with Endogenous Iron in Splenic Macrophages}, author = {Alice Balfourier and Elena Tsolaki and Laura Heeb and Fabian HL Starsich and Daniel Klose and Andreas Boss and Anurag Gupta and Alexander Gogos and Inge K Herrmann}, doi = {10.1002/smtd.202201061}, year = {2022}, date = {2022-01-01}, journal = {Small Methods}, pages = {2201061}, publisher = {Wiley Online Library}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Fate and biological impact of persistent luminescence nanoparticles after injection in mice: a one-year follow-up Article de journal Thomas Lécuyer; Johanne Seguin; Alice Balfourier; Marine Delagrange; Pierre Burckel; René Lai-Kuen; Virginie Mignon; Bertrand Ducos; Michael Tharaud; Bruno Saubaméa; Daniel Scherman; Nathalie Mignet; Florence Gazeau; Cyrille Richard Nanoscale, p. 10.1039.D2NR03546D, 2022, ISSN: 2040-3364, 2040-3372. @article{lecuyer_fate_2022, title = {Fate and biological impact of persistent luminescence nanoparticles after injection in mice: a one-year follow-up}, author = {Thomas L\'{e}cuyer and Johanne Seguin and Alice Balfourier and Marine Delagrange and Pierre Burckel and Ren\'{e} Lai-Kuen and Virginie Mignon and Bertrand Ducos and Michael Tharaud and Bruno Saubam\'{e}a and Daniel Scherman and Nathalie Mignet and Florence Gazeau and Cyrille Richard}, doi = {10.1039/D2NR03546D}, issn = {2040-3364, 2040-3372}, year = {2022}, date = {2022-01-01}, urldate = {2022-10-17}, journal = {Nanoscale}, pages = {10.1039.D2NR03546D}, keywords = {}, pubstate = {published}, tppubtype = {article} } |