Peptides, Glycoconjugates and Metals in Biology

@article{Cois2024,
title = {Design of Bright Chemogenetic Reporters Based on the Combined Engineering of Fluorogenic Molecular Rotors and of the HaloTag Protein},
author = {Justine Co{\"{i}}s and Sylvestre P J T Bachollet and Louis Sanchez and Nicolas Pietrancosta and Vincent Vialou and Jean-Maurice Mallet and Blaise Dumat},
url = {https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/chem.202400641},
doi = {10.1002/chem.202400641},
issn = {0947-6539},
year = {2024},
date = {2024-04-01},
journal = {Chemistry \textendash A European Journal},
abstract = {The combination of fluorogenic probes (fluorogens) and self‐labeling protein tags represent a promising tool for imaging biological processes with high specificity but it requires the adequation between the fluorogen and its target to ensure a good activation of its fluorescence. In this work, we report a strategy to develop molecular rotors that specifically target HaloTag with a strong enhancement of their fluorescence. The divergent design facilitates the diversification of the structures to tune the photophysical and cellular properties. Four bright fluorogens with emissions ranging from green to red were identified and applied in wash‐free live cell imaging experiments with good contrast and selectivity. A HaloTag mutant adapted from previous literature reports was also tested and shown to further improve the brightness and reaction rate of the most promising fluorogen of the series both in vitro and in cells. This work opens new possibilities to develop bright chemogenetic reporters with diverse photophysical and biological properties by exploring a potentially large chemical space of simple dipolar fluorophores in combination with protein engineering.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

@article{Michelis2024,
title = {FRET-Sensing of Multivalent Protein Binding at the Interface of Biomimetic Microparticles Functionalized with Fluorescent Glycolipids},
author = {Sophie Michelis and Chiara Pompili and Florence Niedergang and Jacques Fattaccioli and Blaise Dumat and Jean-Maurice Mallet},
url = {https://pubs.acs.org/doi/10.1021/acsami.3c15067},
doi = {10.1021/acsami.3c15067},
issn = {1944-8244},
year = {2024},
date = {2024-02-01},
journal = {ACS Applied Materials & Interfaces},
volume = {16},
number = {8},
pages = {9669--9679},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

@article{CRCHIM_2024__27_S2_A12_0,
title = {Metal complexes in cells: from design of catalytic antioxidants to imaging metal ions and designing metal-based probes in X-ray fluorescence and IR-imaging, a multidisciplinary collaborative journey in bioinorganic chemistry and inorganic chemical biology},
author = {Clotilde Policar and Nicolas Delsuc and H\'{e}l`ene Charlotte Bertrand},
doi = {10.5802/crchim.295},
year = {2024},
date = {2024-01-01},
journal = {Comptes Rendus. Chimie},
publisher = {Acad\'{e}mie des sciences, Paris},
note = {Online first},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

@article{Bachollet2022b,
title = {Fluorogenic and Genetic Targeting of a Red-Emitting Molecular Calcium Indicator},
author = {Sylvestre P J T Bachollet and Nicolas Pietrancosta and Jean-Maurice Mallet and Blaise Dumat},
doi = {10.1039/D2CC01792J},
year = {2022},
date = {2022-01-01},
journal = {Chemical Communications},
keywords = {},
pubstate = {published},
tppubtype = {article}
}

@article{Bachollet2022,
title = {An expanded palette of fluorogenic HaloTag probes with enhanced contrast for targeted cellular imaging},
author = {Sylvestre P J T Bachollet and Yuriy Shpinov and Fanny Broch and Hela Benaissa and Arnaud Gautier and Nicolas Pietrancosta and Jean-Maurice Mallet and Blaise Dumat},
url = {http://xlink.rsc.org/?DOI=D1OB02394B},
doi = {10.1039/D1OB02394B},
issn = {1477-0520},
year = {2022},
date = {2022-01-01},
journal = {Organic & Biomolecular Chemistry},
volume = {20},
number = {17},
pages = {3619 - 3628},
publisher = {Royal Society of Chemistry},
abstract = {A palette of fluorogenic molecular rotor probes with emissions from green to NIR was developed for wash-free and multicolor imaging of genetically-encoded HaloTag fusion proteins.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}