Coupling Electrochemistry and Fluorescence to Investigate Biological Transport Processes

 
Members involved:
Fatma BEN TRAD, Olivier BURIEZ, Eric LABBE, Jérôme DELACOTTE, Frédéric LEMAITRE, Manon GUILLE-COLLIGNON Justine PANDARD,


(See our publications below)

The cellular entrance and exit of bioactive molecules are key-issues for living organisms since they encompass crucial processes (cell signaling and communication, cellular excretory activity and drug delivery…) Whatever the nature of the uptake/release process, unsolved questions remain on the quantification of the species crossing the plasma membrane and on the description of the mechanistic steps involved at short timescales. Our project relies on the merging and coupling of electrochemical techniques (for the activation & detection of chemical species and for their time resolution) and fluorescence techniques (for their spatial sensitivity & imaging possibilities) to provide a better characterization of dynamic processes related to the internalization and the release of biomolecules – e.g. cell penetrating peptides & neurotransmitters. We propose an integrated approach where the quest for quantitative and time resolved information on transport governs both the design/preparation of adequate redox fluorescent probes and the conception of suitable setups and collecting/recording methodologies. This integrated “from the probe to the application” approach is aimed at making fluorescence and electrochemistry a designable, controllable and quantitative dual tool to explore trans-membrane traffic.


Our publications
:

2019

Electroactive fluorescent false neurotransmitter FFN102 partially replaces dopamine in PC12 cell vesicles Article de journal

L Hu; A Savy; L Grimaud; M Guille-Collignon; F Lemaître; C Amatore; J Delacotte

Biophysical Chemistry, 245 , p. 1–5, 2019.

Résumé | Liens

2018

Redox switchable rhodamine-ferrocene dyad: Exploring imaging possibilities in cells Article de journal

M Čížková; L Cattiaux; J Pandard; M Guille-Collignon; F Lemaître; J Delacotte; J -M Mallet; E Labbé; O Buriez

Electrochemistry Communications, 97 , p. 46–50, 2018.

Résumé | Liens

Electrochemical switching fluorescence emission in rhodamine derivatives Article de journal

M Čížková; L Cattiaux; J -M Mallet; E Labbé; O Buriez

Electrochimica Acta, 260 , p. 589–597, 2018.

Résumé | Liens

Coupling electrochemistry and TIRF-microscopy with the fluorescent false neurotransmitter FFN102 supports the fluorescence signals during single vesicle exocytosis detection Article de journal

X Liu; L Hu; N Pan; L Grimaud; E Labbé; O Buriez; J Delacotte; F Lemaître; M Guille-Collignon

Biophysical Chemistry, 235 , p. 48–55, 2018.

Résumé | Liens

2017

A Dual Functional Electroactive and Fluorescent Probe for Coupled Measurements of Vesicular Exocytosis with High Spatial and Temporal Resolution Article de journal

X Liu; A Savy; S Maurin; L Grimaud; F Darchen; D Quinton; E Labbé; O Buriez; J Delacotte; F Lemaître; M Guille-Collignon

Angewandte Chemie - International Edition, 56 (9), p. 2366–2370, 2017.

Résumé | Liens

Electrochemical quenching of the fluorescence produced by NBD-labelled cell penetrating peptides: A contribution to the study of their internalization in large unilamellar vesicles Article de journal

R de Oliveira; M Durand; L Challier; P Messina; J M Swiecicki; M Di Pisa; G Chassaing; S Lavielle; O Buriez; E Labbé

Journal of Electroanalytical Chemistry, 788 , p. 225–231, 2017.

Résumé | Liens

2015

Bioanalytical applications of the fluorescence-electrochemistry combination Article de journal

F Lemaître; M Guille-Collignon

Actualite Chimique, (400-401), p. 17–19, 2015.

Résumé | Liens