2021
|
An Electrochemical Study of Bis(cyclopentadienyl)titanium(IV) Dichloride in the Presence of Magnesium Ions, Amides or Alkynes Article de journal A K D Dime; Y Six; O Buriez Russian Journal of Electrochemistry, 57 (1), p. 85-91, 2021. @article{,
title = {An Electrochemical Study of Bis(cyclopentadienyl)titanium(IV) Dichloride in the Presence of Magnesium Ions, Amides or Alkynes},
author = {A K D Dime and Y Six and O Buriez},
url = {https://doi.org/10.1134/s1023193521010031},
doi = {10.1134/s1023193521010031},
year = {2021},
date = {2021-01-01},
journal = {Russian Journal of Electrochemistry},
volume = {57},
number = {1},
pages = {85-91},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Electrochemical Fluorescence Switch of Organic Fluorescent or Fluorogenic Molecules Article de journal M Guille-Collignon; J M Delacotte; F Lemaitre; E Labbe; O Buriez Chemical Record, 21 (9), p. 2193-2202, 2021. @article{,
title = {Electrochemical Fluorescence Switch of Organic Fluorescent or Fluorogenic Molecules},
author = {M Guille-Collignon and J M Delacotte and F Lemaitre and E Labbe and O Buriez},
url = {https://doi.org/10.1002/tcr.202100022},
doi = {10.1002/tcr.202100022},
year = {2021},
date = {2021-09-01},
journal = {Chemical Record},
volume = {21},
number = {9},
pages = {2193-2202},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2020
|
Disclosing the redox metabolism of drugs: The essential role of electrochemistry Article de journal O Buriez; E Labbe Current Opinion in Electrochemistry, 24 , p. 63-68, 2020. @article{,
title = {Disclosing the redox metabolism of drugs: The essential role of electrochemistry},
author = {O Buriez and E Labbe},
url = {https://doi.org/10.1016/j.coelec.2020.07.002},
doi = {10.1016/j.coelec.2020.07.002},
year = {2020},
date = {2020-12-01},
journal = {Current Opinion in Electrochemistry},
volume = {24},
pages = {63-68},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
The strong potential of organic and molecular electrochemistry Article de journal O Buriez Current Opinion in Electrochemistry, 24 , p. A1-A3, 2020. @article{,
title = {The strong potential of organic and molecular electrochemistry},
author = {O Buriez},
url = {https://doi.org/10.1016/j.coelec.2020.100657},
doi = {10.1016/j.coelec.2020.100657},
year = {2020},
date = {2020-12-01},
journal = {Current Opinion in Electrochemistry},
volume = {24},
pages = {A1-A3},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2019
|
A Fluorescent False Neurotransmitter as a Dual Electrofluorescent Probe for Secretory Cell Models Article de journal J Pandard; N Pan; D H Ebene; T Le Saux; E Ait-Yahiatène; X Liu; L Grimaud; O Buriez; E Labbé; F Lemaître; M Guille-Collignon ChemPlusChem, 84 (10), p. 1578-1586, 2019, (cited By 0). @article{Pandard20191578,
title = {A Fluorescent False Neurotransmitter as a Dual Electrofluorescent Probe for Secretory Cell Models},
author = {J Pandard and N Pan and D H Ebene and T Le Saux and E Ait-Yahiat\`{e}ne and X Liu and L Grimaud and O Buriez and E Labb\'{e} and F Lema\^{i}tre and M Guille-Collignon},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85073931778&doi=10.1002%2fcplu.201900385&partnerID=40&md5=a526291d966e7dc926b44e97c73794b3},
doi = {10.1002/cplu.201900385},
year = {2019},
date = {2019-01-01},
journal = {ChemPlusChem},
volume = {84},
number = {10},
pages = {1578-1586},
note = {cited By 0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Cover Feature: Fundamental Input of Analytical Electrochemistry in the Determination of Intermediates and Reaction Mechanisms in Electrosynthetic Processes Article de journal E Labbe; O Buriez Chemelectrochem, 6 (16), p. 4061, 2019. @article{,
title = {Cover Feature: Fundamental Input of Analytical Electrochemistry in the Determination of Intermediates and Reaction Mechanisms in Electrosynthetic Processes},
author = {E Labbe and O Buriez},
url = {https://doi.org/10.1002/celc.201901158},
doi = {10.1002/celc.201901158},
year = {2019},
date = {2019-01-01},
journal = {Chemelectrochem},
volume = {6},
number = {16},
pages = {4061},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Diverting photosynthetic electrons from suspensions of Chlamydomonas reinhardtii algae - New insights using an electrochemical well device Article de journal A Sayegh; G Longatte; O Buriez; F -A Wollman; M Guille-Collignon; E Labbé; J Delacotte; F Lemaître Electrochimica Acta, 304 , p. 465 - 473, 2019, ISSN: 0013-4686. @article{SAYEGH2019465,
title = {Diverting photosynthetic electrons from suspensions of Chlamydomonas reinhardtii algae - New insights using an electrochemical well device},
author = {A Sayegh and G Longatte and O Buriez and F -A Wollman and M Guille-Collignon and E Labb\'{e} and J Delacotte and F Lema\^{i}tre},
url = {http://www.sciencedirect.com/science/article/pii/S0013468619303718},
doi = {https://doi.org/10.1016/j.electacta.2019.02.105},
issn = {0013-4686},
year = {2019},
date = {2019-01-01},
journal = {Electrochimica Acta},
volume = {304},
pages = {465 - 473},
abstract = {In the last years, many strategies have been developed to benefit from oxygenic photosynthesis in the present context of renewable energies. To achieve this, bioelectricity may be produced by using photosynthetic components involved in anodic or cathodic compartments. In this respect, harvesting photosynthetic electrons from living biological systems appears to be an encouraging approach. However it raises the question of the most suitable electrochemical device. In this work, we describe and analyze the performances of an electrochemical device based on a millimeter sized well involving a gold surface as a working electrode. Photocurrents were generated by suspensions of Chlamydomonas reinhardtii algae using quinones as mediators under different experimental conditions. Chronoamperometry and cyclic voltammetry measurements gave insight into the use of this device to investigate important issues (harvesting and poisoning by quinones, photoinactivation…). Furthermore, by introducing a kinetic model originally developed for homogeneous catalytic systems, the kinetics of the electron diverting from this system (Chlamydomonas reinhardtii algae + 2,6-DCBQ + miniaturized setup) can be estimated. All these results demonstrate that this experimental configuration is suitable for future works devoted to the choice of the best parameters in terms of long lasting performances.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In the last years, many strategies have been developed to benefit from oxygenic photosynthesis in the present context of renewable energies. To achieve this, bioelectricity may be produced by using photosynthetic components involved in anodic or cathodic compartments. In this respect, harvesting photosynthetic electrons from living biological systems appears to be an encouraging approach. However it raises the question of the most suitable electrochemical device. In this work, we describe and analyze the performances of an electrochemical device based on a millimeter sized well involving a gold surface as a working electrode. Photocurrents were generated by suspensions of Chlamydomonas reinhardtii algae using quinones as mediators under different experimental conditions. Chronoamperometry and cyclic voltammetry measurements gave insight into the use of this device to investigate important issues (harvesting and poisoning by quinones, photoinactivation…). Furthermore, by introducing a kinetic model originally developed for homogeneous catalytic systems, the kinetics of the electron diverting from this system (Chlamydomonas reinhardtii algae + 2,6-DCBQ + miniaturized setup) can be estimated. All these results demonstrate that this experimental configuration is suitable for future works devoted to the choice of the best parameters in terms of long lasting performances. |
Fundamental Input of Analytical Electrochemistry in the Determination of Intermediates and Reaction Mechanisms in Electrosynthetic Processes Article de journal E Labbé; O Buriez ChemElectroChem, 6 (16), p. 4118-4125, 2019, (cited By 1). @article{Labb\'{e}20194118,
title = {Fundamental Input of Analytical Electrochemistry in the Determination of Intermediates and Reaction Mechanisms in Electrosynthetic Processes},
author = {E Labb\'{e} and O Buriez},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85063908009&doi=10.1002%2fcelc.201900045&partnerID=40&md5=be86c0958f347ea6b4d1d62a0526e44c},
doi = {10.1002/celc.201900045},
year = {2019},
date = {2019-01-01},
journal = {ChemElectroChem},
volume = {6},
number = {16},
pages = {4118-4125},
note = {cited By 1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
New Mechanistic Insights into Osmium-Based Tamoxifen Derivatives Article de journal H Z S Lee; F Chau; S Top; G Jaouen; A Vessieres; E Labbe; O Buriez Electrochimica Acta, 302 , p. 130-136, 2019, ISSN: 0013-4686. @article{RN52,
title = {New Mechanistic Insights into Osmium-Based Tamoxifen Derivatives},
author = {H Z S Lee and F Chau and S Top and G Jaouen and A Vessieres and E Labbe and O Buriez},
doi = {10.1016/j.electacta.2019.02.019},
issn = {0013-4686},
year = {2019},
date = {2019-01-01},
journal = {Electrochimica Acta},
volume = {302},
pages = {130-136},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2018
|
Coupling electrochemistry and TIRF-microscopy with the fluorescent false neurotransmitter FFN102 supports the fluorescence signals during single vesicle exocytosis detection Article de journal X Liu; L Hu; N Pan; L Grimaud; E Labbé; O Buriez; J Delacotte; F Lemaître; M Guille-Collignon Biophysical Chemistry, 235 , p. 48–55, 2018. @article{Liu:2018,
title = {Coupling electrochemistry and TIRF-microscopy with the fluorescent false neurotransmitter FFN102 supports the fluorescence signals during single vesicle exocytosis detection},
author = {X Liu and L Hu and N Pan and L Grimaud and E Labb\'{e} and O Buriez and J Delacotte and F Lema\^{i}tre and M Guille-Collignon},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042352158&doi=10.1016%2fj.bpc.2018.02.004&partnerID=40&md5=365430d79a0d526895e755729264d88f},
doi = {10.1016/j.bpc.2018.02.004},
year = {2018},
date = {2018-01-01},
journal = {Biophysical Chemistry},
volume = {235},
pages = {48--55},
abstract = {Applications of the Fluorescent False Neurotransmitter FFN102, an analog of biogenic neurotransmitters and a suitable probe for coupled amperometry and TIRFM (total internal reflexion fluorescence microscopy) investigations of exocytotic secretion, were considered here. The electroactivity of FFN102 was shown to very likely arise from the oxidation of its phenolic group through a CE (Chemical-Electrochemical) mechanism. Evidences that the aminoethyl group of FFN102 is the key recognition element by BON N13 cells were also provided. Amperometric measurements were then performed at the single cell level with carbon fiber electrode (CFE) or Indium Tin Oxide (ITO) surfaces. It proved the disparity of kinetic and quantitative parameters of FFN102-stained cells acquired either at cell top and bottom. Moreover, coupled analyses of FFN102 loaded vesicles allowed us to classify three types of optical signals that probably arise from secretion releases thanks to their concomitant detection with an electrochemical spike. Finally, preliminary benefits from the coupling involving FFN102 were reported in terms of origins of overlapped amperometric spikes or assignment of fluorescence extinctions to real exocytotic events. © 2018 Elsevier B.V.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Applications of the Fluorescent False Neurotransmitter FFN102, an analog of biogenic neurotransmitters and a suitable probe for coupled amperometry and TIRFM (total internal reflexion fluorescence microscopy) investigations of exocytotic secretion, were considered here. The electroactivity of FFN102 was shown to very likely arise from the oxidation of its phenolic group through a CE (Chemical-Electrochemical) mechanism. Evidences that the aminoethyl group of FFN102 is the key recognition element by BON N13 cells were also provided. Amperometric measurements were then performed at the single cell level with carbon fiber electrode (CFE) or Indium Tin Oxide (ITO) surfaces. It proved the disparity of kinetic and quantitative parameters of FFN102-stained cells acquired either at cell top and bottom. Moreover, coupled analyses of FFN102 loaded vesicles allowed us to classify three types of optical signals that probably arise from secretion releases thanks to their concomitant detection with an electrochemical spike. Finally, preliminary benefits from the coupling involving FFN102 were reported in terms of origins of overlapped amperometric spikes or assignment of fluorescence extinctions to real exocytotic events. © 2018 Elsevier B.V. |
Electrochemical switching fluorescence emission in rhodamine derivatives Article de journal M Čížková; L Cattiaux; J -M Mallet; E Labbé; O Buriez Electrochimica Acta, 260 , p. 589–597, 2018. @article{Cizkova:2018a,
title = {Electrochemical switching fluorescence emission in rhodamine derivatives},
author = {M \v{C}\'{i}\v{z}kov\'{a} and L Cattiaux and J -M Mallet and E Labb\'{e} and O Buriez},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85038870042&doi=10.1016%2fj.electacta.2017.12.104&partnerID=40&md5=6a1537d7de8ad37549ef796b0c6f2642},
doi = {10.1016/j.electacta.2017.12.104},
year = {2018},
date = {2018-01-01},
journal = {Electrochimica Acta},
volume = {260},
pages = {589--597},
abstract = {Three rhodamine derivatives exhibiting electrofluorochromic properties were investigated by cyclic voltammetry and UV\textendashVis/fluorescence spectroelectrochemistry. Rhodamine 101 (Rh101, compound 1) was used as a reference model. In compound 2, the carboxylate anion of Rh101 was replaced by an alkyne moiety to allow further functionalization. The compound 3 was prepared from 2 by conversion of the alkyne to a triazole group bearing an alkyl chain with an alcohol function. These three rhodamine derivatives exhibited similar electrochemical behaviors. Their mono-electronic reductions produced the corresponding radical species which were stable on the time-scale of cyclic voltammetry. Additional reduction of electrogenerated radicals produced unstable anions which underwent subsequent chemical reaction, most likely protonation. Based on cyclic voltammetry investigations, absorption and fluorescence spectroelectrochemistry were then performed on compounds 1, 2, 3 and their parent reduced radicals 1a, 2a, 3a. UV\textendashVis spectroelectrochemistry, combined with TD-DFT calculation, confirmed the formation of radicals upon mono-electronic reduction of starting rhodamines. Fluorescence spectroelectrochemistry showed that, contrary to their parent molecules, electrogenerated radicals were non-fluorescent. Electrochemical fluorescence extinction was successfully achieved with all studied compounds. Moreover, compound 1 underwent on/off switching between fluorescent and non-fluorescent states repeatedly. Also, recovery of fluorescence in compound 3 was observed, which open interesting opportunities for the development of versatile rhodamine-based probes. © 2017 The Authors},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Three rhodamine derivatives exhibiting electrofluorochromic properties were investigated by cyclic voltammetry and UV–Vis/fluorescence spectroelectrochemistry. Rhodamine 101 (Rh101, compound 1) was used as a reference model. In compound 2, the carboxylate anion of Rh101 was replaced by an alkyne moiety to allow further functionalization. The compound 3 was prepared from 2 by conversion of the alkyne to a triazole group bearing an alkyl chain with an alcohol function. These three rhodamine derivatives exhibited similar electrochemical behaviors. Their mono-electronic reductions produced the corresponding radical species which were stable on the time-scale of cyclic voltammetry. Additional reduction of electrogenerated radicals produced unstable anions which underwent subsequent chemical reaction, most likely protonation. Based on cyclic voltammetry investigations, absorption and fluorescence spectroelectrochemistry were then performed on compounds 1, 2, 3 and their parent reduced radicals 1a, 2a, 3a. UV–Vis spectroelectrochemistry, combined with TD-DFT calculation, confirmed the formation of radicals upon mono-electronic reduction of starting rhodamines. Fluorescence spectroelectrochemistry showed that, contrary to their parent molecules, electrogenerated radicals were non-fluorescent. Electrochemical fluorescence extinction was successfully achieved with all studied compounds. Moreover, compound 1 underwent on/off switching between fluorescent and non-fluorescent states repeatedly. Also, recovery of fluorescence in compound 3 was observed, which open interesting opportunities for the development of versatile rhodamine-based probes. © 2017 The Authors |
Fast and complete electrochemical conversion of solutes contained in micro-volume water droplets Article de journal L Godeffroy; F Chau; O Buriez; E Labbé Electrochemistry Communications, 86 , p. 145–148, 2018. @article{Godeffroy:2018,
title = {Fast and complete electrochemical conversion of solutes contained in micro-volume water droplets},
author = {L Godeffroy and F Chau and O Buriez and E Labb\'{e}},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85038019547&doi=10.1016%2fj.elecom.2017.12.007&partnerID=40&md5=e4be97ff9584068f86e05922768a8567},
doi = {10.1016/j.elecom.2017.12.007},
year = {2018},
date = {2018-01-01},
journal = {Electrochemistry Communications},
volume = {86},
pages = {145--148},
abstract = {An elegant hanging-droplet or meniscus-based setup is proposed to carry out quantitative electrolyses from either an organic (hydroquinone) or an inorganic (permanganate) substrate. These examples validate the concept of using such easily accessible, fast (1\textendash3 min) and low-cost operating conditions not only for preparative applications (electrosynthesis), but also for pedagogical purposes in minute samples. © 2017 Elsevier B.V.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
An elegant hanging-droplet or meniscus-based setup is proposed to carry out quantitative electrolyses from either an organic (hydroquinone) or an inorganic (permanganate) substrate. These examples validate the concept of using such easily accessible, fast (1–3 min) and low-cost operating conditions not only for preparative applications (electrosynthesis), but also for pedagogical purposes in minute samples. © 2017 Elsevier B.V. |
Redox switchable rhodamine-ferrocene dyad: Exploring imaging possibilities in cells Article de journal M Čížková; L Cattiaux; J Pandard; M Guille-Collignon; F Lemaître; J Delacotte; J -M Mallet; E Labbé; O Buriez Electrochemistry Communications, 97 , p. 46–50, 2018. @article{Cizkova:2018,
title = {Redox switchable rhodamine-ferrocene dyad: Exploring imaging possibilities in cells},
author = {M \v{C}\'{i}\v{z}kov\'{a} and L Cattiaux and J Pandard and M Guille-Collignon and F Lema\^{i}tre and J Delacotte and J -M Mallet and E Labb\'{e} and O Buriez},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85054592456&doi=10.1016%2fj.elecom.2018.10.009&partnerID=40&md5=10a4aed1c89bb6a788a2a260bbd0a818},
doi = {10.1016/j.elecom.2018.10.009},
year = {2018},
date = {2018-01-01},
journal = {Electrochemistry Communications},
volume = {97},
pages = {46--50},
abstract = {An original redox-responsive fluorescent probe combining a rhodamine derivative and a ferrocenyl moiety used as the fluorescence modulator was designed, synthesized and characterized. The fluorescence of this new dyad could be tuned from the redox state of ferrocene, a feature observed both electrochemically and on cancer cells incubated with this probe. © 2018 Elsevier B.V.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
An original redox-responsive fluorescent probe combining a rhodamine derivative and a ferrocenyl moiety used as the fluorescence modulator was designed, synthesized and characterized. The fluorescence of this new dyad could be tuned from the redox state of ferrocene, a feature observed both electrochemically and on cancer cells incubated with this probe. © 2018 Elsevier B.V. |
2017
|
A Dual Functional Electroactive and Fluorescent Probe for Coupled Measurements of Vesicular Exocytosis with High Spatial and Temporal Resolution Article de journal X Liu; A Savy; S Maurin; L Grimaud; F Darchen; D Quinton; E Labbé; O Buriez; J Delacotte; F Lemaître; M Guille-Collignon Angewandte Chemie - International Edition, 56 (9), p. 2366–2370, 2017. @article{Liu:2017a,
title = {A Dual Functional Electroactive and Fluorescent Probe for Coupled Measurements of Vesicular Exocytosis with High Spatial and Temporal Resolution},
author = {X Liu and A Savy and S Maurin and L Grimaud and F Darchen and D Quinton and E Labb\'{e} and O Buriez and J Delacotte and F Lema\^{i}tre and M Guille-Collignon},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85010696856&doi=10.1002%2fanie.201611145&partnerID=40&md5=a51767157166d7f185f0195a28b347b8},
doi = {10.1002/anie.201611145},
year = {2017},
date = {2017-01-01},
journal = {Angewandte Chemie - International Edition},
volume = {56},
number = {9},
pages = {2366--2370},
abstract = {In this work, Fluorescent False Neurotransmitter 102 (FFN102), a synthesized analogue of biogenic neurotransmitters, was demonstrated to show both pH-dependent fluorescence and electroactivity. To study secretory behaviors at the single-vesicle level, FFN102 was employed as a new fluorescent/electroactive dual probe in a coupled technique (amperometry and total internal reflection fluorescence microscopy (TIRFM)). We used N13 cells, a stable clone of BON cells, to specifically accumulate FFN102 into their secretory vesicles, and then optical and electrochemical measurements of vesicular exocytosis were experimentally achieved by using indium tin oxide (ITO) transparent electrodes. Upon stimulation, FFN102 started to diffuse out from the acidic intravesicular microenvironment to the neutral extracellular space, leading to fluorescent emissions and to the electrochemical oxidation signals that were simultaneously collected from the ITO electrode surface. The correlation of fluorescence and amperometric signals resulting from the FFN102 probe allows real-time monitoring of single exocytotic events with both high spatial and temporal resolution. This work opens new possibilities in the investigation of exocytotic mechanisms. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In this work, Fluorescent False Neurotransmitter 102 (FFN102), a synthesized analogue of biogenic neurotransmitters, was demonstrated to show both pH-dependent fluorescence and electroactivity. To study secretory behaviors at the single-vesicle level, FFN102 was employed as a new fluorescent/electroactive dual probe in a coupled technique (amperometry and total internal reflection fluorescence microscopy (TIRFM)). We used N13 cells, a stable clone of BON cells, to specifically accumulate FFN102 into their secretory vesicles, and then optical and electrochemical measurements of vesicular exocytosis were experimentally achieved by using indium tin oxide (ITO) transparent electrodes. Upon stimulation, FFN102 started to diffuse out from the acidic intravesicular microenvironment to the neutral extracellular space, leading to fluorescent emissions and to the electrochemical oxidation signals that were simultaneously collected from the ITO electrode surface. The correlation of fluorescence and amperometric signals resulting from the FFN102 probe allows real-time monitoring of single exocytotic events with both high spatial and temporal resolution. This work opens new possibilities in the investigation of exocytotic mechanisms. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim |
Electrochemical quenching of the fluorescence produced by NBD-labelled cell penetrating peptides: A contribution to the study of their internalization in large unilamellar vesicles Article de journal R de Oliveira; M Durand; L Challier; P Messina; J M Swiecicki; M Di Pisa; G Chassaing; S Lavielle; O Buriez; E Labbé Journal of Electroanalytical Chemistry, 788 , p. 225–231, 2017. @article{deOliveira:2017,
title = {Electrochemical quenching of the fluorescence produced by NBD-labelled cell penetrating peptides: A contribution to the study of their internalization in large unilamellar vesicles},
author = {R de Oliveira and M Durand and L Challier and P Messina and J M Swiecicki and M Di Pisa and G Chassaing and S Lavielle and O Buriez and E Labb\'{e}},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85013176758&doi=10.1016%2fj.jelechem.2017.02.006&partnerID=40&md5=725e8c07b1f4090ecf4f2ceabb57e6f7},
doi = {10.1016/j.jelechem.2017.02.006},
year = {2017},
date = {2017-01-01},
journal = {Journal of Electroanalytical Chemistry},
volume = {788},
pages = {225--231},
abstract = {This work investigates the implementation of a simple and versatile electrochemical setup aimed at achieving a fast and complete fluorescence extinction of NBD-labelled (NBD = 7-nitrobenz-2-oxa-1,3-diazole) cell penetrating peptides contained in 2\textendash5 cm3 samples containing phosphate buffer + large unilamellar vesicles. The quenching is obtained through a reductive electrolysis in a 2-compartment cell homebuilt from disposable plastic labware, which remains inert towards the adsorption of both peptides and lipid vesicles. Considering the micromolar concentration of NBD-tagged peptides, the main electrochemical reaction observed is hydrogen evolution, NBD reduction representing a small fraction of the cathodic current/charge engaged. The electrolysis conditions are discussed with respect to the nature of the reduction products formed, the integrity of large unilamellar vesicles and phosphate buffering properties. This electrochemical method is compared to the traditional chemical dithionite quenching of NBD and tested to monitor the internalization of cell penetrating peptides in large unilamellar vesicles. © 2017 Elsevier B.V.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
This work investigates the implementation of a simple and versatile electrochemical setup aimed at achieving a fast and complete fluorescence extinction of NBD-labelled (NBD = 7-nitrobenz-2-oxa-1,3-diazole) cell penetrating peptides contained in 2–5 cm3 samples containing phosphate buffer + large unilamellar vesicles. The quenching is obtained through a reductive electrolysis in a 2-compartment cell homebuilt from disposable plastic labware, which remains inert towards the adsorption of both peptides and lipid vesicles. Considering the micromolar concentration of NBD-tagged peptides, the main electrochemical reaction observed is hydrogen evolution, NBD reduction representing a small fraction of the cathodic current/charge engaged. The electrolysis conditions are discussed with respect to the nature of the reduction products formed, the integrity of large unilamellar vesicles and phosphate buffering properties. This electrochemical method is compared to the traditional chemical dithionite quenching of NBD and tested to monitor the internalization of cell penetrating peptides in large unilamellar vesicles. © 2017 Elsevier B.V. |
