2022
|
An expanded palette of fluorogenic HaloTag probes with enhanced contrast for targeted cellular imaging Article de journal Sylvestre P J T Bachollet; Yuriy Shpinov; Fanny Broch; Hela Benaissa; Arnaud Gautier; Nicolas Pietrancosta; Jean-Maurice Mallet; Blaise Dumat Organic & Biomolecular Chemistry, 20 (17), p. 3619 - 3628, 2022, ISSN: 1477-0520. @article{Bachollet2022,
title = {An expanded palette of fluorogenic HaloTag probes with enhanced contrast for targeted cellular imaging},
author = {Sylvestre P J T Bachollet and Yuriy Shpinov and Fanny Broch and Hela Benaissa and Arnaud Gautier and Nicolas Pietrancosta and Jean-Maurice Mallet and Blaise Dumat},
url = {http://xlink.rsc.org/?DOI=D1OB02394B},
doi = {10.1039/D1OB02394B},
issn = {1477-0520},
year = {2022},
date = {2022-01-01},
journal = {Organic & Biomolecular Chemistry},
volume = {20},
number = {17},
pages = {3619 - 3628},
publisher = {Royal Society of Chemistry},
abstract = {A palette of fluorogenic molecular rotor probes with emissions from green to NIR was developed for wash-free and multicolor imaging of genetically-encoded HaloTag fusion proteins.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
A palette of fluorogenic molecular rotor probes with emissions from green to NIR was developed for wash-free and multicolor imaging of genetically-encoded HaloTag fusion proteins. |
Transition kinetics of mixed lipid:photosurfactant assemblies studied by time-resolved small angle X-ray scattering Article de journal Jorges Royes; V. A. Bjørnestad; G. Brun; Th. Narayanan; R Lund; C Tribet J Colloid Interface Sci., 610 , p. 830-841, 2022, ISBN: 0021-9797. @article{Royes2022,
title = {Transition kinetics of mixed lipid:photosurfactant assemblies studied by time-resolved small angle X-ray scattering },
author = {Jorges Royes and V. A. Bj\ornestad and G. Brun and Th. Narayanan and R Lund and C Tribet},
editor = {Elsevier},
url = {https://www.sciencedirect.com/science/article/abs/pii/S0021979721020439?via%3Dihub},
doi = {10.1016/j.jcis.2021.11.133 },
isbn = {0021-9797},
year = {2022},
date = {2022-03-15},
journal = {J Colloid Interface Sci.},
volume = {610},
pages = {830-841},
abstract = {Hypothesis: Photoswitchable surfactants are used in the design of many light-responsive colloids and/or self-assemblies. Photo-isomerization enables to control molecular equilibrium, and triggers transient reorganizations with possibly out-of-equilibrium intermediate states that have been overlooked. Here, we address this question by an in depth structural investigation of intermediate lipid-surfactant assemblies that occur during fast isothermal photo-triggered transition in lipid:surfactant mixtures.
Experiments: The structural parameters of mixed assemblies of azobenzene-containing cationic surfactant (AzoTMA) and dioleoylphosphatidylcholine (DOPC) lipids were studied by light scattering and time-resolved small angle X-ray scattering. Structural and compositional information about the assemblies and unimers in bulk were determined at the photostationary states, as well as at intermediate kinetic states formed during UV or blue light illumination.
Findings: DOPC:AzoTMA systems form mixed assemblies representative of phospholipid:cationic surfactant mixtures, that evolve from spheroid, to rod-like micelles, and vesicles with increasing DOPC fraction. Transient assemblies detected during the photo-triggered kinetics are similar to the ones found in stationary states. But changes of AzoTMA unimers in bulk can be considerably faster than mass reorganizations of the mixed assemblies, suggesting that out-of-equilibrium conditions are transiently reached. Mass reorganization of the surfactant-enriched assemblies is much faster than in the lipid enriched ones, providing insight into the role of lipids in a slow reorganization of the assemblies.
},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Hypothesis: Photoswitchable surfactants are used in the design of many light-responsive colloids and/or self-assemblies. Photo-isomerization enables to control molecular equilibrium, and triggers transient reorganizations with possibly out-of-equilibrium intermediate states that have been overlooked. Here, we address this question by an in depth structural investigation of intermediate lipid-surfactant assemblies that occur during fast isothermal photo-triggered transition in lipid:surfactant mixtures.
Experiments: The structural parameters of mixed assemblies of azobenzene-containing cationic surfactant (AzoTMA) and dioleoylphosphatidylcholine (DOPC) lipids were studied by light scattering and time-resolved small angle X-ray scattering. Structural and compositional information about the assemblies and unimers in bulk were determined at the photostationary states, as well as at intermediate kinetic states formed during UV or blue light illumination.
Findings: DOPC:AzoTMA systems form mixed assemblies representative of phospholipid:cationic surfactant mixtures, that evolve from spheroid, to rod-like micelles, and vesicles with increasing DOPC fraction. Transient assemblies detected during the photo-triggered kinetics are similar to the ones found in stationary states. But changes of AzoTMA unimers in bulk can be considerably faster than mass reorganizations of the mixed assemblies, suggesting that out-of-equilibrium conditions are transiently reached. Mass reorganization of the surfactant-enriched assemblies is much faster than in the lipid enriched ones, providing insight into the role of lipids in a slow reorganization of the assemblies.
|
UCST-Type Polymer Capsules Formed by Interfacial Complexation Article de journal L Sixdenier; A Auge; Y Zhao; E Marie; C Tribet ACS MACRO LETTERS, 11 (5), p. 651-656, 2022, ISBN: 2161-1653 J9 - ACS MACRO LETT. @article{Sixdenier2022,
title = {UCST-Type Polymer Capsules Formed by Interfacial Complexation},
author = {L Sixdenier and A Auge and Y Zhao and E Marie and C Tribet},
doi = {10.1021/acsmacrolett.2c00021},
isbn = {2161-1653 J9 - ACS MACRO LETT},
year = {2022},
date = {2022-05-17},
journal = {ACS MACRO LETTERS},
volume = {11},
number = {5},
pages = {651-656},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2021
|
An Electrochemical Study of Bis(cyclopentadienyl)titanium(IV) Dichloride in the Presence of Magnesium Ions, Amides or Alkynes Article de journal A K D Dime; Y Six; O Buriez Russian Journal of Electrochemistry, 57 (1), p. 85-91, 2021. @article{,
title = {An Electrochemical Study of Bis(cyclopentadienyl)titanium(IV) Dichloride in the Presence of Magnesium Ions, Amides or Alkynes},
author = {A K D Dime and Y Six and O Buriez},
url = {https://doi.org/10.1134/s1023193521010031},
doi = {10.1134/s1023193521010031},
year = {2021},
date = {2021-01-01},
journal = {Russian Journal of Electrochemistry},
volume = {57},
number = {1},
pages = {85-91},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Electrochemical Fluorescence Switch of Organic Fluorescent or Fluorogenic Molecules Article de journal M Guille-Collignon; J M Delacotte; F Lemaitre; E Labbe; O Buriez Chemical Record, 21 (9), p. 2193-2202, 2021. @article{,
title = {Electrochemical Fluorescence Switch of Organic Fluorescent or Fluorogenic Molecules},
author = {M Guille-Collignon and J M Delacotte and F Lemaitre and E Labbe and O Buriez},
url = {https://doi.org/10.1002/tcr.202100022},
doi = {10.1002/tcr.202100022},
year = {2021},
date = {2021-09-01},
journal = {Chemical Record},
volume = {21},
number = {9},
pages = {2193-2202},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Emulsion-Templated Poly(N-Isopropylacrylamide) Shells Formed by Thermo-Enhanced Interfacial Complexation Article de journal L Sixdenier; C Tribet; E Marie ADVANCED FUNCTIONAL MATERIALS, 31 (51), p. 2105490, 2021, ISBN: 1616-301X 1616-3028 J9 - ADV FUNCT MATER. @article{Sixdenier2021b,
title = {Emulsion-Templated Poly(N-Isopropylacrylamide) Shells Formed by Thermo-Enhanced Interfacial Complexation},
author = {L Sixdenier and C Tribet and E Marie},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.202105490},
doi = {10.1002/adfm.202105490},
isbn = {1616-301X 1616-3028 J9 - ADV FUNCT MATER},
year = {2021},
date = {2021-09-17},
journal = {ADVANCED FUNCTIONAL MATERIALS},
volume = {31},
number = {51},
pages = {2105490},
abstract = {The encapsulation of fragile biomacromolecules is crucial in many biotechnological applications but remains challenging. Interfacial complexation (IC) in water-in-oil emulsions proves to be an efficient process for the formation of protective polymer layers at the surface of capsule-precursor water droplets. In this work, the enhancement of conventional IC by introducing thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) strands in the interfacial polymer layer is described. Surfactant-polymer IC is implemented in water-in-fluorocarbon oil emulsions between a water-soluble poly(L-lysine)-g-poly(N-isopropylacrylamide) cationic copolymer (PLL-g-PNIPAM) and an oil-soluble anionic surfactant. Fluorescence imaging demonstrates that the thermal collapse transition of PNIPAM strands, triggered by gentle heating, induces an enrichment of the polymer layer initially formed by IC. Spontaneous co-precipitation of nanoparticles initially dispersed in the aqueous cores-with no specific treatment-is also achieved upon PNIPAM transition. This process is leveraged to irreversibly segregate these nanoparticles in the interfacial polymer layer, resulting in gel-like mixed shells. Thermo-enhancement of conventional IC is thus a promising approach for the straightforward formation, strengthening, and functionalization of capsule shells. As implemented in mild conditions, thermo-enhanced IC is additionally compatible with the encapsulation of proteins, opening new opportunities for delivery systems of biomacromolecules.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The encapsulation of fragile biomacromolecules is crucial in many biotechnological applications but remains challenging. Interfacial complexation (IC) in water-in-oil emulsions proves to be an efficient process for the formation of protective polymer layers at the surface of capsule-precursor water droplets. In this work, the enhancement of conventional IC by introducing thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) strands in the interfacial polymer layer is described. Surfactant-polymer IC is implemented in water-in-fluorocarbon oil emulsions between a water-soluble poly(L-lysine)-g-poly(N-isopropylacrylamide) cationic copolymer (PLL-g-PNIPAM) and an oil-soluble anionic surfactant. Fluorescence imaging demonstrates that the thermal collapse transition of PNIPAM strands, triggered by gentle heating, induces an enrichment of the polymer layer initially formed by IC. Spontaneous co-precipitation of nanoparticles initially dispersed in the aqueous cores-with no specific treatment-is also achieved upon PNIPAM transition. This process is leveraged to irreversibly segregate these nanoparticles in the interfacial polymer layer, resulting in gel-like mixed shells. Thermo-enhancement of conventional IC is thus a promising approach for the straightforward formation, strengthening, and functionalization of capsule shells. As implemented in mild conditions, thermo-enhanced IC is additionally compatible with the encapsulation of proteins, opening new opportunities for delivery systems of biomacromolecules. |
Finding Adapted Quinones for Harvesting Electrons from Photosynthetic Algae Suspensions Article de journal A Sayegh; L A Perego; M A Romero; L Escudero; J Delacotte; M Guille-Collignon; L Grimaud; B Bailleul; F Lemaitre Chemelectrochem, 8 (15), p. 2968-2978, 2021. @article{,
title = {Finding Adapted Quinones for Harvesting Electrons from Photosynthetic Algae Suspensions},
author = {A Sayegh and L A Perego and M A Romero and L Escudero and J Delacotte and M Guille-Collignon and L Grimaud and B Bailleul and F Lemaitre},
url = {https://doi.org/10.1002/celc.202100757},
doi = {10.1002/celc.202100757},
year = {2021},
date = {2021-08-01},
journal = {Chemelectrochem},
volume = {8},
number = {15},
pages = {2968-2978},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Finding Adapted Quinones for Harvesting Electrons from Photosynthetic Algae Suspensions Article de journal A Sayegh; L A Perego; M A Romero; L Escudero; J Delacotte; M Guille-Collignon; L Grimaud; B Bailleul; F Lemaitre Chemelectrochem, 8 (15), p. 2968-2978, 2021. @article{,
title = {Finding Adapted Quinones for Harvesting Electrons from Photosynthetic Algae Suspensions},
author = {A Sayegh and L A Perego and M A Romero and L Escudero and J Delacotte and M Guille-Collignon and L Grimaud and B Bailleul and F Lemaitre},
url = {https://doi.org/10.1002/celc.202100757},
doi = {10.1002/celc.202100757},
year = {2021},
date = {2021-08-01},
journal = {Chemelectrochem},
volume = {8},
number = {15},
pages = {2968-2978},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Recent Developments Concerning the Investigation of Exocytosis with Amperometry Article de journal M Guille-Collignon; F Lemaitre Current Opinion in Electrochemistry, 29 , p. 100751, 2021. @article{,
title = {Recent Developments Concerning the Investigation of Exocytosis with Amperometry},
author = {M Guille-Collignon and F Lemaitre},
url = {https://doi.org/10.1016/j.coelec.2021.100751},
doi = {10.1016/j.coelec.2021.100751},
year = {2021},
date = {2021-01-01},
journal = {Current Opinion in Electrochemistry},
volume = {29},
pages = {100751},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Simulations of amperometric monitoring of exocytosis: moderate pH variations within the cell-electrode cleft with the buffer diffusion Article de journal Y Bouret; M Guille-Collignon; F Lemaitre Analytical and Bioanalytical Chemistry, 2021. @article{,
title = {Simulations of amperometric monitoring of exocytosis: moderate pH variations within the cell-electrode cleft with the buffer diffusion},
author = {Y Bouret and M Guille-Collignon and F Lemaitre},
url = {https://doi.org/10.1007/s00216-021-03443-z},
doi = {10.1007/s00216-021-03443-z},
year = {2021},
date = {2021-01-01},
journal = {Analytical and Bioanalytical Chemistry},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Ultrafast Dynamics of Fully Reduced Flavin in Catalytic Structures of Thymidylate Synthase ThyX Article de journal Nadia Dozova; Fabien Lacombat; Murielle Lombard; Djemel Hamdane; Pascal Plaza Physical Chemistry Chemical Physics, 23 , p. 22692-22702, 2021, ISSN: 1463-9076. @article{RN123b,
title = {Ultrafast Dynamics of Fully Reduced Flavin in Catalytic Structures of Thymidylate Synthase ThyX},
author = {Nadia Dozova and Fabien Lacombat and Murielle Lombard and Djemel Hamdane and Pascal Plaza},
url = {http://dx.doi.org/10.1039/D1CP03379D},
doi = {10.1039/D1CP03379D},
issn = {1463-9076},
year = {2021},
date = {2021-01-01},
journal = {Physical Chemistry Chemical Physics},
volume = {23},
pages = {22692-22702},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Ultrafast photoreduction dynamics of a new class of CPD photolyases Article de journal Fabien Lacombat; Agathe Espagne; Nadia Dozova; Pascal Plaza; Pavel Müller; Hans-Joachim Emmerich; Martin Saft; Lars-Oliver Essen Photochemical & Photobiological Sciences, 20 (6), p. 733-746, 2021, ISSN: 1474-9092. @article{RN632,
title = {Ultrafast photoreduction dynamics of a new class of CPD photolyases},
author = {Fabien Lacombat and Agathe Espagne and Nadia Dozova and Pascal Plaza and Pavel M\"{u}ller and Hans-Joachim Emmerich and Martin Saft and Lars-Oliver Essen},
url = {https://doi.org/10.1007/s43630-021-00048-4},
doi = {10.1007/s43630-021-00048-4},
issn = {1474-9092},
year = {2021},
date = {2021-01-01},
journal = {Photochemical & Photobiological Sciences},
volume = {20},
number = {6},
pages = {733-746},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2020
|
Disclosing the redox metabolism of drugs: The essential role of electrochemistry Article de journal O Buriez; E Labbe Current Opinion in Electrochemistry, 24 , p. 63-68, 2020. @article{,
title = {Disclosing the redox metabolism of drugs: The essential role of electrochemistry},
author = {O Buriez and E Labbe},
url = {https://doi.org/10.1016/j.coelec.2020.07.002},
doi = {10.1016/j.coelec.2020.07.002},
year = {2020},
date = {2020-12-01},
journal = {Current Opinion in Electrochemistry},
volume = {24},
pages = {63-68},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Dynamic contrast for overcoming spectral interferences in fluorescence imaging Article de journal R Chouket; A Pellissier-Tanon; A Lemarchand; A Espagne; T Le Saux; L Jullien Journal of Physics-Photonics, 2 (3), p. 8, 2020, ISSN: 2515-7647. @article{RN1_30,
title = {Dynamic contrast for overcoming spectral interferences in fluorescence imaging},
author = {R Chouket and A Pellissier-Tanon and A Lemarchand and A Espagne and T Le Saux and L Jullien},
url = {<Go to ISI>://WOS:000572940200001},
doi = {10.1088/2515-7647/ab9099},
issn = {2515-7647},
year = {2020},
date = {2020-01-01},
journal = {Journal of Physics-Photonics},
volume = {2},
number = {3},
pages = {8},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Dynamic contrast with reversibly photoswitchable fluorescent labels for imaging living cells Article de journal R Chouket; A Pellissier-Tanon; A Lemarchand; A Espagne; T Le Saux; L Jullien Chemical Science, 11 (11), p. 2882-2887, 2020, ISSN: 2041-6520. @article{RN2x,
title = {Dynamic contrast with reversibly photoswitchable fluorescent labels for imaging living cells},
author = {R Chouket and A Pellissier-Tanon and A Lemarchand and A Espagne and T Le Saux and L Jullien},
url = {<Go to ISI>://WOS:000521247400002},
doi = {10.1039/d0sc00182a},
issn = {2041-6520},
year = {2020},
date = {2020-01-01},
journal = {Chemical Science},
volume = {11},
number = {11},
pages = {2882-2887},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Inducible intracellular membranes: molecular aspects and emerging applications Article de journal J Royes; V Biou; N Dautin; C Tribet; B Miroux Microbial Cell Factories, 19 (1), 2020. @article{RN263,
title = {Inducible intracellular membranes: molecular aspects and emerging applications},
author = {J Royes and V Biou and N Dautin and C Tribet and B Miroux},
url = {<Go to ISI>://WOS:000568404600002},
doi = {10.1186/s12934-020-01433-x},
year = {2020},
date = {2020-01-01},
journal = {Microbial Cell Factories},
volume = {19},
number = {1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Mediator-Microorganism Interaction in Microbial Solar Cell: a Fluo-Electrochemical Insight Article de journal L Beauzamy; J Delacotte; B Bailleul; K Tanaka; S Nakanishi; F A Wollman; F Lemaitre Analytical Chemistry, 92 (11), p. 7532-7539, 2020. @article{,
title = {Mediator-Microorganism Interaction in Microbial Solar Cell: a Fluo-Electrochemical Insight},
author = {L Beauzamy and J Delacotte and B Bailleul and K Tanaka and S Nakanishi and F A Wollman and F Lemaitre},
url = {https://doi.org/10.1021/acs.analchem.9b05808},
doi = {10.1021/acs.analchem.9b05808},
year = {2020},
date = {2020-06-01},
journal = {Analytical Chemistry},
volume = {92},
number = {11},
pages = {7532-7539},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Overview and outlook of the strategies devoted to electrofluorescence surveys: Application to single cell secretion analysis Article de journal M Guille-Collignon; F Lemaitre Trac-Trends in Analytical Chemistry, 132 , p. 116055, 2020. @article{,
title = {Overview and outlook of the strategies devoted to electrofluorescence surveys: Application to single cell secretion analysis},
author = {M Guille-Collignon and F Lemaitre},
url = {https://doi.org/10.1016/j.trac.2020.116055},
doi = {10.1016/j.trac.2020.116055},
year = {2020},
date = {2020-11-01},
journal = {Trac-Trends in Analytical Chemistry},
volume = {132},
pages = {116055},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Solubilization and Stabilization of Membrane Proteins by Cycloalkane-Modified Amphiphilic Polymers Article de journal Anaïs Marconnet; Baptiste Michon; Christel Le Bon; Fabrice Giusti; Christophe Tribet; Manuela Zoonens Biomacromolecules, 21 (8), p. 3459-3467, 2020, ISSN: 1525-7797. @article{Marconnet2020,
title = {Solubilization and Stabilization of Membrane Proteins by Cycloalkane-Modified Amphiphilic Polymers},
author = {Ana\"{i}s Marconnet and Baptiste Michon and Christel Le Bon and Fabrice Giusti and Christophe Tribet and Manuela Zoonens },
editor = {American Chemical Society},
url = {https://doi.org/10.1021/acs.biomac.0c00929},
doi = {10.1021/acs.biomac.0c00929},
issn = {1525-7797},
year = {2020},
date = {2020-08-10},
journal = {Biomacromolecules},
volume = {21},
number = {8},
pages = {3459-3467},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
The strong potential of organic and molecular electrochemistry Article de journal O Buriez Current Opinion in Electrochemistry, 24 , p. A1-A3, 2020. @article{,
title = {The strong potential of organic and molecular electrochemistry},
author = {O Buriez},
url = {https://doi.org/10.1016/j.coelec.2020.100657},
doi = {10.1016/j.coelec.2020.100657},
year = {2020},
date = {2020-12-01},
journal = {Current Opinion in Electrochemistry},
volume = {24},
pages = {A1-A3},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Underlying mechanisms in microbial solar cells: how modeling can help Article de journal L Beauzamy; F Lemaitre; J Derr Sustainable Energy & Fuels, 4 (12), p. 6004-6010, 2020. @article{,
title = {Underlying mechanisms in microbial solar cells: how modeling can help},
author = {L Beauzamy and F Lemaitre and J Derr},
url = {https://doi.org/10.1039/d0se01304h},
doi = {10.1039/d0se01304h},
year = {2020},
date = {2020-12-01},
journal = {Sustainable Energy & Fuels},
volume = {4},
number = {12},
pages = {6004-6010},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2019
|
A Fluorescent False Neurotransmitter as a Dual Electrofluorescent Probe for Secretory Cell Models Article de journal J Pandard; N Pan; D H Ebene; T Le Saux; E Ait-Yahiatène; X Liu; L Grimaud; O Buriez; E Labbé; F Lemaître; M Guille-Collignon ChemPlusChem, 84 (10), p. 1578-1586, 2019, (cited By 0). @article{Pandard20191578,
title = {A Fluorescent False Neurotransmitter as a Dual Electrofluorescent Probe for Secretory Cell Models},
author = {J Pandard and N Pan and D H Ebene and T Le Saux and E Ait-Yahiat\`{e}ne and X Liu and L Grimaud and O Buriez and E Labb\'{e} and F Lema\^{i}tre and M Guille-Collignon},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85073931778&doi=10.1002%2fcplu.201900385&partnerID=40&md5=a526291d966e7dc926b44e97c73794b3},
doi = {10.1002/cplu.201900385},
year = {2019},
date = {2019-01-01},
journal = {ChemPlusChem},
volume = {84},
number = {10},
pages = {1578-1586},
note = {cited By 0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
A split fluorescent reporter with rapid and reversible complementation Article de journal Alison G Tebo; Arnaud Gautier Nature Communications, 10 (1), p. 2822, 2019, ISSN: 2041-1723. @article{Tebo2019,
title = {A split fluorescent reporter with rapid and reversible complementation},
author = {Alison G Tebo and Arnaud Gautier},
url = {https://doi.org/10.1038/s41467-019-10855-0},
doi = {10.1038/s41467-019-10855-0},
issn = {2041-1723},
year = {2019},
date = {2019-01-01},
journal = {Nature Communications},
volume = {10},
number = {1},
pages = {2822},
abstract = {Interactions between proteins play an essential role in metabolic and signaling pathways, cellular processes and organismal systems. We report the development of splitFAST, a fluorescence complementation system for the visualization of transient protein-protein interactions in living cells. Engineered from the fluorogenic reporter FAST (Fluorescence-Activating and absorption-Shifting Tag), which specifically and reversibly binds fluorogenic hydroxybenzylidene rhodanine (HBR) analogs, splitFAST displays rapid and reversible complementation, allowing the real-time visualization of both the formation and the dissociation of a protein assembly.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Interactions between proteins play an essential role in metabolic and signaling pathways, cellular processes and organismal systems. We report the development of splitFAST, a fluorescence complementation system for the visualization of transient protein-protein interactions in living cells. Engineered from the fluorogenic reporter FAST (Fluorescence-Activating and absorption-Shifting Tag), which specifically and reversibly binds fluorogenic hydroxybenzylidene rhodanine (HBR) analogs, splitFAST displays rapid and reversible complementation, allowing the real-time visualization of both the formation and the dissociation of a protein assembly. |
Bacteria-Based Production of Thiol-Clickable, Genetically Encoded Lipid Nanovesicles Article de journal Jorge Royes; Oana Ilioaia; Quentin Lubart; Federica Angius; Galina V Dubacheva; Marta Bally; Bruno Miroux; Christophe Tribet Angewandte Chemie International Edition, 58 , p. 7395-7399, 2019, ISSN: 1433-7851. @article{RN1x,
title = {Bacteria-Based Production of Thiol-Clickable, Genetically Encoded Lipid Nanovesicles},
author = {Jorge Royes and Oana Ilioaia and Quentin Lubart and Federica Angius and Galina V Dubacheva and Marta Bally and Bruno Miroux and Christophe Tribet},
doi = {10.1002/anie.201902929},
issn = {1433-7851},
year = {2019},
date = {2019-01-01},
journal = {Angewandte Chemie International Edition},
volume = {58},
pages = {7395-7399},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Cover Feature: Fundamental Input of Analytical Electrochemistry in the Determination of Intermediates and Reaction Mechanisms in Electrosynthetic Processes Article de journal E Labbe; O Buriez Chemelectrochem, 6 (16), p. 4061, 2019. @article{,
title = {Cover Feature: Fundamental Input of Analytical Electrochemistry in the Determination of Intermediates and Reaction Mechanisms in Electrosynthetic Processes},
author = {E Labbe and O Buriez},
url = {https://doi.org/10.1002/celc.201901158},
doi = {10.1002/celc.201901158},
year = {2019},
date = {2019-01-01},
journal = {Chemelectrochem},
volume = {6},
number = {16},
pages = {4061},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Distinctive Low-Resolution Structural Features of Dimers of Antibody–Drug Conjugates and Parent Antibody Determined by Small-Angle X-ray Scattering Article de journal Didier Law-Hine; Sergii Rudiuk; Audrey Bonestebe; Romain Ienco; Sylvain Huille; Christophe Tribet Mol. Pharmaceutics, 16 (12), p. 4902-4912, 2019. @article{Law-Hine2019,
title = {Distinctive Low-Resolution Structural Features of Dimers of Antibody\textendashDrug Conjugates and Parent Antibody Determined by Small-Angle X-ray Scattering},
author = {Didier Law-Hine and Sergii Rudiuk and Audrey Bonestebe and Romain Ienco and Sylvain Huille and Christophe Tribet},
doi = {10.1021/acs.molpharmaceut.9b00792},
year = {2019},
date = {2019-10-16},
journal = {Mol. Pharmaceutics},
volume = {16},
number = {12},
pages = {4902-4912},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Diverting photosynthetic electrons from suspensions of Chlamydomonas reinhardtii algae - New insights using an electrochemical well device Article de journal A Sayegh; G Longatte; O Buriez; F -A Wollman; M Guille-Collignon; E Labbé; J Delacotte; F Lemaître Electrochimica Acta, 304 , p. 465 - 473, 2019, ISSN: 0013-4686. @article{SAYEGH2019465,
title = {Diverting photosynthetic electrons from suspensions of Chlamydomonas reinhardtii algae - New insights using an electrochemical well device},
author = {A Sayegh and G Longatte and O Buriez and F -A Wollman and M Guille-Collignon and E Labb\'{e} and J Delacotte and F Lema\^{i}tre},
url = {http://www.sciencedirect.com/science/article/pii/S0013468619303718},
doi = {https://doi.org/10.1016/j.electacta.2019.02.105},
issn = {0013-4686},
year = {2019},
date = {2019-01-01},
journal = {Electrochimica Acta},
volume = {304},
pages = {465 - 473},
abstract = {In the last years, many strategies have been developed to benefit from oxygenic photosynthesis in the present context of renewable energies. To achieve this, bioelectricity may be produced by using photosynthetic components involved in anodic or cathodic compartments. In this respect, harvesting photosynthetic electrons from living biological systems appears to be an encouraging approach. However it raises the question of the most suitable electrochemical device. In this work, we describe and analyze the performances of an electrochemical device based on a millimeter sized well involving a gold surface as a working electrode. Photocurrents were generated by suspensions of Chlamydomonas reinhardtii algae using quinones as mediators under different experimental conditions. Chronoamperometry and cyclic voltammetry measurements gave insight into the use of this device to investigate important issues (harvesting and poisoning by quinones, photoinactivation…). Furthermore, by introducing a kinetic model originally developed for homogeneous catalytic systems, the kinetics of the electron diverting from this system (Chlamydomonas reinhardtii algae + 2,6-DCBQ + miniaturized setup) can be estimated. All these results demonstrate that this experimental configuration is suitable for future works devoted to the choice of the best parameters in terms of long lasting performances.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In the last years, many strategies have been developed to benefit from oxygenic photosynthesis in the present context of renewable energies. To achieve this, bioelectricity may be produced by using photosynthetic components involved in anodic or cathodic compartments. In this respect, harvesting photosynthetic electrons from living biological systems appears to be an encouraging approach. However it raises the question of the most suitable electrochemical device. In this work, we describe and analyze the performances of an electrochemical device based on a millimeter sized well involving a gold surface as a working electrode. Photocurrents were generated by suspensions of Chlamydomonas reinhardtii algae using quinones as mediators under different experimental conditions. Chronoamperometry and cyclic voltammetry measurements gave insight into the use of this device to investigate important issues (harvesting and poisoning by quinones, photoinactivation…). Furthermore, by introducing a kinetic model originally developed for homogeneous catalytic systems, the kinetics of the electron diverting from this system (Chlamydomonas reinhardtii algae + 2,6-DCBQ + miniaturized setup) can be estimated. All these results demonstrate that this experimental configuration is suitable for future works devoted to the choice of the best parameters in terms of long lasting performances. |
Electroactive fluorescent false neurotransmitter FFN102 partially replaces dopamine in PC12 cell vesicles Article de journal L Hu; A Savy; L Grimaud; M Guille-Collignon; F Lemaître; C Amatore; J Delacotte Biophysical Chemistry, 245 , p. 1–5, 2019. @article{Hu:2019,
title = {Electroactive fluorescent false neurotransmitter FFN102 partially replaces dopamine in PC12 cell vesicles},
author = {L Hu and A Savy and L Grimaud and M Guille-Collignon and F Lema\^{i}tre and C Amatore and J Delacotte},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85057201704&doi=10.1016%2fj.bpc.2018.11.001&partnerID=40&md5=28655b4c152ce0fc51fc037feefcff97},
doi = {10.1016/j.bpc.2018.11.001},
year = {2019},
date = {2019-01-01},
journal = {Biophysical Chemistry},
volume = {245},
pages = {1--5},
abstract = {In the last decade, following fluorescent dyes and protein tags, pH sensitive false fluorescent neurotransmitters (FFN) were introduced and were valuable for labeling secretory vesicles and monitoring exocytosis at living cells. In particular, the synthetic analog of neurotransmitters FFN102 was shown to be an electroactive probe. Here, we show that FFN102 is suitable to be used as a bioanalytic probe at the widely used PC12 cell model. FFN102 was uptaken in the secretory vesicles of PC12 cells, partially replacing the endogenous dopamine stored in these vesicles. The different oxidation potentials of dopamine and FFN102 allowed to determine that ca. 12% of dopamine was replaced by FFN102. Moreover, the FFN102 was found to be over released through the initial fusion pore suggesting that it was mostly uptaken in fast diffusion compartment of the vesicles. © 2018 Elsevier B.V.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In the last decade, following fluorescent dyes and protein tags, pH sensitive false fluorescent neurotransmitters (FFN) were introduced and were valuable for labeling secretory vesicles and monitoring exocytosis at living cells. In particular, the synthetic analog of neurotransmitters FFN102 was shown to be an electroactive probe. Here, we show that FFN102 is suitable to be used as a bioanalytic probe at the widely used PC12 cell model. FFN102 was uptaken in the secretory vesicles of PC12 cells, partially replacing the endogenous dopamine stored in these vesicles. The different oxidation potentials of dopamine and FFN102 allowed to determine that ca. 12% of dopamine was replaced by FFN102. Moreover, the FFN102 was found to be over released through the initial fusion pore suggesting that it was mostly uptaken in fast diffusion compartment of the vesicles. © 2018 Elsevier B.V. |
Fundamental Input of Analytical Electrochemistry in the Determination of Intermediates and Reaction Mechanisms in Electrosynthetic Processes Article de journal E Labbé; O Buriez ChemElectroChem, 6 (16), p. 4118-4125, 2019, (cited By 1). @article{Labb\'{e}20194118,
title = {Fundamental Input of Analytical Electrochemistry in the Determination of Intermediates and Reaction Mechanisms in Electrosynthetic Processes},
author = {E Labb\'{e} and O Buriez},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85063908009&doi=10.1002%2fcelc.201900045&partnerID=40&md5=be86c0958f347ea6b4d1d62a0526e44c},
doi = {10.1002/celc.201900045},
year = {2019},
date = {2019-01-01},
journal = {ChemElectroChem},
volume = {6},
number = {16},
pages = {4118-4125},
note = {cited By 1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Live Cell Super Resolution Imaging by Radial Fluctuations Using Fluorogen Binding Tags Article de journal Muthukumaran Venkatachalapathy; Vivek Belapurkar; Mini Jose; Arnaud Gautier; Deepak Nair Nanoscale, 11 (8), p. 3626-3632, 2019, ISSN: 2040-3364. @article{RN45,
title = {Live Cell Super Resolution Imaging by Radial Fluctuations Using Fluorogen Binding Tags},
author = {Muthukumaran Venkatachalapathy and Vivek Belapurkar and Mini Jose and Arnaud Gautier and Deepak Nair},
doi = {10.1039/C8NR07809B},
issn = {2040-3364},
year = {2019},
date = {2019-01-01},
journal = {Nanoscale},
volume = {11},
number = {8},
pages = {3626-3632},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
