You will find below the publication list of our pole.
For the publication list of each pole member, please see his/her personal webpage.
2023 |
Multiscale Transient Absorption Study of the Fluorescent Protein Dreiklang and Two Point Variants Provides Insight into Photoswitching and Nonproductive Reaction Pathways Article de journal Emilie Renouard; Magdalena Nowinska; Fabien Lacombat; Pascal Plaza; Pavel Müller; Agathe Espagne The Journal of Physical Chemistry Letters, 14 (28), p. 6477-6485, 2023. @article{RN63c, title = {Multiscale Transient Absorption Study of the Fluorescent Protein Dreiklang and Two Point Variants Provides Insight into Photoswitching and Nonproductive Reaction Pathways}, author = {Emilie Renouard and Magdalena Nowinska and Fabien Lacombat and Pascal Plaza and Pavel M\"{u}ller and Agathe Espagne}, url = {https://doi.org/10.1021/acs.jpclett.3c00431}, doi = {10.1021/acs.jpclett.3c00431}, year = {2023}, date = {2023-01-01}, journal = {The Journal of Physical Chemistry Letters}, volume = {14}, number = {28}, pages = {6477-6485}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Near-infrared co-illumination of fluorescent proteins reduces photobleaching and phototoxicity Article de journal L Ludvikova; E Simon; M Deygas; T Panier; M A Plamont; J Ollion; A Tebo; M Piel; L Jullien; L Robert; T Le Saux; A Espagne Nature Biotechnology, p. 12, 2023, ISSN: 1087-0156. @article{RN156, title = {Near-infrared co-illumination of fluorescent proteins reduces photobleaching and phototoxicity}, author = {L Ludvikova and E Simon and M Deygas and T Panier and M A Plamont and J Ollion and A Tebo and M Piel and L Jullien and L Robert and T Le Saux and A Espagne}, url = {<Go to ISI>://WOS:001042699900001}, doi = {10.1038/s41587-023-01893-7}, issn = {1087-0156}, year = {2023}, date = {2023-01-01}, journal = {Nature Biotechnology}, pages = {12}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2022 |
An expanded palette of fluorogenic HaloTag probes with enhanced contrast for targeted cellular imaging Article de journal Sylvestre P J T Bachollet; Yuriy Shpinov; Fanny Broch; Hela Benaissa; Arnaud Gautier; Nicolas Pietrancosta; Jean-Maurice Mallet; Blaise Dumat Organic & Biomolecular Chemistry, 20 (17), p. 3619 - 3628, 2022, ISSN: 1477-0520. @article{Bachollet2022, title = {An expanded palette of fluorogenic HaloTag probes with enhanced contrast for targeted cellular imaging}, author = {Sylvestre P J T Bachollet and Yuriy Shpinov and Fanny Broch and Hela Benaissa and Arnaud Gautier and Nicolas Pietrancosta and Jean-Maurice Mallet and Blaise Dumat}, url = {http://xlink.rsc.org/?DOI=D1OB02394B}, doi = {10.1039/D1OB02394B}, issn = {1477-0520}, year = {2022}, date = {2022-01-01}, journal = {Organic & Biomolecular Chemistry}, volume = {20}, number = {17}, pages = {3619 - 3628}, publisher = {Royal Society of Chemistry}, abstract = {A palette of fluorogenic molecular rotor probes with emissions from green to NIR was developed for wash-free and multicolor imaging of genetically-encoded HaloTag fusion proteins.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A palette of fluorogenic molecular rotor probes with emissions from green to NIR was developed for wash-free and multicolor imaging of genetically-encoded HaloTag fusion proteins. |
Reciprocal Regulation of Shh Trafficking and H2O2 Levels via a Noncanonical BOC-Rac1 Pathway Article de journal Marion Thauvin; Irène Amblard; Christine Rampon; Aurélien Mourton; Isabelle Queguiner; Chenge Li; Arnaud Gautier; Alain Joliot; Michel Volovitch; Sophie Vriz Antioxidants (Basel, Switzerland), 11 (4), p. 718, 2022, ISSN: 2076-3921. @article{thauvin_reciprocal_2022, title = {Reciprocal Regulation of Shh Trafficking and H2O2 Levels via a Noncanonical BOC-Rac1 Pathway}, author = {Marion Thauvin and Ir\`{e}ne Amblard and Christine Rampon and Aur\'{e}lien Mourton and Isabelle Queguiner and Chenge Li and Arnaud Gautier and Alain Joliot and Michel Volovitch and Sophie Vriz}, doi = {10.3390/antiox11040718}, issn = {2076-3921}, year = {2022}, date = {2022-01-01}, journal = {Antioxidants (Basel, Switzerland)}, volume = {11}, number = {4}, pages = {718}, abstract = {Among molecules that bridge environment, cell metabolism, and cell signaling, hydrogen peroxide (H2O2) recently appeared as an emerging but central player. Its level depends on cell metabolism and environment and was recently shown to play key roles during embryogenesis, contrasting with its long-established role in disease progression. We decided to explore whether the secreted morphogen Sonic hedgehog (Shh), known to be essential in a variety of biological processes ranging from embryonic development to adult tissue homeostasis and cancers, was part of these interactions. Here, we report that H2O2 levels control key steps of Shh delivery in cell culture: increased levels reduce primary secretion, stimulate endocytosis and accelerate delivery to recipient cells; in addition, physiological in vivo modulation of H2O2 levels changes Shh distribution and tissue patterning. Moreover, a feedback loop exists in which Shh trafficking controls H2O2 synthesis via a non-canonical BOC-Rac1 pathway, leading to cytoneme growth. Our findings reveal that Shh directly impacts its own distribution, thus providing a molecular explanation for the robustness of morphogenesis to both environmental insults and individual variability.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Among molecules that bridge environment, cell metabolism, and cell signaling, hydrogen peroxide (H2O2) recently appeared as an emerging but central player. Its level depends on cell metabolism and environment and was recently shown to play key roles during embryogenesis, contrasting with its long-established role in disease progression. We decided to explore whether the secreted morphogen Sonic hedgehog (Shh), known to be essential in a variety of biological processes ranging from embryonic development to adult tissue homeostasis and cancers, was part of these interactions. Here, we report that H2O2 levels control key steps of Shh delivery in cell culture: increased levels reduce primary secretion, stimulate endocytosis and accelerate delivery to recipient cells; in addition, physiological in vivo modulation of H2O2 levels changes Shh distribution and tissue patterning. Moreover, a feedback loop exists in which Shh trafficking controls H2O2 synthesis via a non-canonical BOC-Rac1 pathway, leading to cytoneme growth. Our findings reveal that Shh directly impacts its own distribution, thus providing a molecular explanation for the robustness of morphogenesis to both environmental insults and individual variability. |
Transition kinetics of mixed lipid:photosurfactant assemblies studied by time-resolved small angle X-ray scattering Article de journal Jorges Royes; V. A. Bjørnestad; G. Brun; Th. Narayanan; R Lund; C Tribet J Colloid Interface Sci., 610 , p. 830-841, 2022, ISBN: 0021-9797. @article{Royes2022, title = {Transition kinetics of mixed lipid:photosurfactant assemblies studied by time-resolved small angle X-ray scattering }, author = {Jorges Royes and V. A. Bj\ornestad and G. Brun and Th. Narayanan and R Lund and C Tribet}, editor = {Elsevier}, url = {https://www.sciencedirect.com/science/article/abs/pii/S0021979721020439?via%3Dihub}, doi = {10.1016/j.jcis.2021.11.133 }, isbn = {0021-9797}, year = {2022}, date = {2022-03-15}, journal = {J Colloid Interface Sci.}, volume = {610}, pages = {830-841}, abstract = {Hypothesis: Photoswitchable surfactants are used in the design of many light-responsive colloids and/or self-assemblies. Photo-isomerization enables to control molecular equilibrium, and triggers transient reorganizations with possibly out-of-equilibrium intermediate states that have been overlooked. Here, we address this question by an in depth structural investigation of intermediate lipid-surfactant assemblies that occur during fast isothermal photo-triggered transition in lipid:surfactant mixtures. Experiments: The structural parameters of mixed assemblies of azobenzene-containing cationic surfactant (AzoTMA) and dioleoylphosphatidylcholine (DOPC) lipids were studied by light scattering and time-resolved small angle X-ray scattering. Structural and compositional information about the assemblies and unimers in bulk were determined at the photostationary states, as well as at intermediate kinetic states formed during UV or blue light illumination. Findings: DOPC:AzoTMA systems form mixed assemblies representative of phospholipid:cationic surfactant mixtures, that evolve from spheroid, to rod-like micelles, and vesicles with increasing DOPC fraction. Transient assemblies detected during the photo-triggered kinetics are similar to the ones found in stationary states. But changes of AzoTMA unimers in bulk can be considerably faster than mass reorganizations of the mixed assemblies, suggesting that out-of-equilibrium conditions are transiently reached. Mass reorganization of the surfactant-enriched assemblies is much faster than in the lipid enriched ones, providing insight into the role of lipids in a slow reorganization of the assemblies. }, keywords = {}, pubstate = {published}, tppubtype = {article} } Hypothesis: Photoswitchable surfactants are used in the design of many light-responsive colloids and/or self-assemblies. Photo-isomerization enables to control molecular equilibrium, and triggers transient reorganizations with possibly out-of-equilibrium intermediate states that have been overlooked. Here, we address this question by an in depth structural investigation of intermediate lipid-surfactant assemblies that occur during fast isothermal photo-triggered transition in lipid:surfactant mixtures. Experiments: The structural parameters of mixed assemblies of azobenzene-containing cationic surfactant (AzoTMA) and dioleoylphosphatidylcholine (DOPC) lipids were studied by light scattering and time-resolved small angle X-ray scattering. Structural and compositional information about the assemblies and unimers in bulk were determined at the photostationary states, as well as at intermediate kinetic states formed during UV or blue light illumination. Findings: DOPC:AzoTMA systems form mixed assemblies representative of phospholipid:cationic surfactant mixtures, that evolve from spheroid, to rod-like micelles, and vesicles with increasing DOPC fraction. Transient assemblies detected during the photo-triggered kinetics are similar to the ones found in stationary states. But changes of AzoTMA unimers in bulk can be considerably faster than mass reorganizations of the mixed assemblies, suggesting that out-of-equilibrium conditions are transiently reached. Mass reorganization of the surfactant-enriched assemblies is much faster than in the lipid enriched ones, providing insight into the role of lipids in a slow reorganization of the assemblies. |
UCST-Type Polymer Capsules Formed by Interfacial Complexation Article de journal L Sixdenier; A Auge; Y Zhao; E Marie; C Tribet ACS MACRO LETTERS, 11 (5), p. 651-656, 2022, ISBN: 2161-1653 J9 - ACS MACRO LETT. @article{Sixdenier2022, title = {UCST-Type Polymer Capsules Formed by Interfacial Complexation}, author = {L Sixdenier and A Auge and Y Zhao and E Marie and C Tribet}, doi = {10.1021/acsmacrolett.2c00021}, isbn = {2161-1653 J9 - ACS MACRO LETT}, year = {2022}, date = {2022-05-17}, journal = {ACS MACRO LETTERS}, volume = {11}, number = {5}, pages = {651-656}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2021 |
An Electrochemical Study of Bis(cyclopentadienyl)titanium(IV) Dichloride in the Presence of Magnesium Ions, Amides or Alkynes Article de journal A K D Dime; Y Six; O Buriez Russian Journal of Electrochemistry, 57 (1), p. 85-91, 2021. @article{, title = {An Electrochemical Study of Bis(cyclopentadienyl)titanium(IV) Dichloride in the Presence of Magnesium Ions, Amides or Alkynes}, author = {A K D Dime and Y Six and O Buriez}, url = {https://doi.org/10.1134/s1023193521010031}, doi = {10.1134/s1023193521010031}, year = {2021}, date = {2021-01-01}, journal = {Russian Journal of Electrochemistry}, volume = {57}, number = {1}, pages = {85-91}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Electrochemical Fluorescence Switch of Organic Fluorescent or Fluorogenic Molecules Article de journal M Guille-Collignon; J M Delacotte; F Lemaitre; E Labbe; O Buriez Chemical Record, 21 (9), p. 2193-2202, 2021. @article{, title = {Electrochemical Fluorescence Switch of Organic Fluorescent or Fluorogenic Molecules}, author = {M Guille-Collignon and J M Delacotte and F Lemaitre and E Labbe and O Buriez}, url = {https://doi.org/10.1002/tcr.202100022}, doi = {10.1002/tcr.202100022}, year = {2021}, date = {2021-09-01}, journal = {Chemical Record}, volume = {21}, number = {9}, pages = {2193-2202}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Emulsion-Templated Poly(N-Isopropylacrylamide) Shells Formed by Thermo-Enhanced Interfacial Complexation Article de journal L Sixdenier; C Tribet; E Marie ADVANCED FUNCTIONAL MATERIALS, 31 (51), p. 2105490, 2021, ISBN: 1616-301X 1616-3028 J9 - ADV FUNCT MATER. @article{Sixdenier2021b, title = {Emulsion-Templated Poly(N-Isopropylacrylamide) Shells Formed by Thermo-Enhanced Interfacial Complexation}, author = {L Sixdenier and C Tribet and E Marie}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.202105490}, doi = {10.1002/adfm.202105490}, isbn = {1616-301X 1616-3028 J9 - ADV FUNCT MATER}, year = {2021}, date = {2021-09-17}, journal = {ADVANCED FUNCTIONAL MATERIALS}, volume = {31}, number = {51}, pages = {2105490}, abstract = {The encapsulation of fragile biomacromolecules is crucial in many biotechnological applications but remains challenging. Interfacial complexation (IC) in water-in-oil emulsions proves to be an efficient process for the formation of protective polymer layers at the surface of capsule-precursor water droplets. In this work, the enhancement of conventional IC by introducing thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) strands in the interfacial polymer layer is described. Surfactant-polymer IC is implemented in water-in-fluorocarbon oil emulsions between a water-soluble poly(L-lysine)-g-poly(N-isopropylacrylamide) cationic copolymer (PLL-g-PNIPAM) and an oil-soluble anionic surfactant. Fluorescence imaging demonstrates that the thermal collapse transition of PNIPAM strands, triggered by gentle heating, induces an enrichment of the polymer layer initially formed by IC. Spontaneous co-precipitation of nanoparticles initially dispersed in the aqueous cores-with no specific treatment-is also achieved upon PNIPAM transition. This process is leveraged to irreversibly segregate these nanoparticles in the interfacial polymer layer, resulting in gel-like mixed shells. Thermo-enhancement of conventional IC is thus a promising approach for the straightforward formation, strengthening, and functionalization of capsule shells. As implemented in mild conditions, thermo-enhanced IC is additionally compatible with the encapsulation of proteins, opening new opportunities for delivery systems of biomacromolecules.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The encapsulation of fragile biomacromolecules is crucial in many biotechnological applications but remains challenging. Interfacial complexation (IC) in water-in-oil emulsions proves to be an efficient process for the formation of protective polymer layers at the surface of capsule-precursor water droplets. In this work, the enhancement of conventional IC by introducing thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) strands in the interfacial polymer layer is described. Surfactant-polymer IC is implemented in water-in-fluorocarbon oil emulsions between a water-soluble poly(L-lysine)-g-poly(N-isopropylacrylamide) cationic copolymer (PLL-g-PNIPAM) and an oil-soluble anionic surfactant. Fluorescence imaging demonstrates that the thermal collapse transition of PNIPAM strands, triggered by gentle heating, induces an enrichment of the polymer layer initially formed by IC. Spontaneous co-precipitation of nanoparticles initially dispersed in the aqueous cores-with no specific treatment-is also achieved upon PNIPAM transition. This process is leveraged to irreversibly segregate these nanoparticles in the interfacial polymer layer, resulting in gel-like mixed shells. Thermo-enhancement of conventional IC is thus a promising approach for the straightforward formation, strengthening, and functionalization of capsule shells. As implemented in mild conditions, thermo-enhanced IC is additionally compatible with the encapsulation of proteins, opening new opportunities for delivery systems of biomacromolecules. |
Finding Adapted Quinones for Harvesting Electrons from Photosynthetic Algae Suspensions Article de journal A Sayegh; L A Perego; M A Romero; L Escudero; J Delacotte; M Guille-Collignon; L Grimaud; B Bailleul; F Lemaitre Chemelectrochem, 8 (15), p. 2968-2978, 2021. @article{, title = {Finding Adapted Quinones for Harvesting Electrons from Photosynthetic Algae Suspensions}, author = {A Sayegh and L A Perego and M A Romero and L Escudero and J Delacotte and M Guille-Collignon and L Grimaud and B Bailleul and F Lemaitre}, url = {https://doi.org/10.1002/celc.202100757}, doi = {10.1002/celc.202100757}, year = {2021}, date = {2021-08-01}, journal = {Chemelectrochem}, volume = {8}, number = {15}, pages = {2968-2978}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Finding Adapted Quinones for Harvesting Electrons from Photosynthetic Algae Suspensions Article de journal A Sayegh; L A Perego; M A Romero; L Escudero; J Delacotte; M Guille-Collignon; L Grimaud; B Bailleul; F Lemaitre Chemelectrochem, 8 (15), p. 2968-2978, 2021. @article{, title = {Finding Adapted Quinones for Harvesting Electrons from Photosynthetic Algae Suspensions}, author = {A Sayegh and L A Perego and M A Romero and L Escudero and J Delacotte and M Guille-Collignon and L Grimaud and B Bailleul and F Lemaitre}, url = {https://doi.org/10.1002/celc.202100757}, doi = {10.1002/celc.202100757}, year = {2021}, date = {2021-08-01}, journal = {Chemelectrochem}, volume = {8}, number = {15}, pages = {2968-2978}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Orthogonal fluorescent chemogenetic reporters for multicolor imaging Article de journal Alison G Tebo; Benjamien Moeyaert; Marion Thauvin; Irene Carlon-Andres; Dorothea Böken; Michel Volovitch; Sergi Padilla-Parra; Peter Dedecker; Sophie Vriz; Arnaud Gautier Nature Chemical Biology, 17 (1), p. 30–38, 2021, ISSN: 1552-4469. @article{tebo_orthogonal_2021, title = {Orthogonal fluorescent chemogenetic reporters for multicolor imaging}, author = {Alison G Tebo and Benjamien Moeyaert and Marion Thauvin and Irene Carlon-Andres and Dorothea B\"{o}ken and Michel Volovitch and Sergi Padilla-Parra and Peter Dedecker and Sophie Vriz and Arnaud Gautier}, doi = {10.1038/s41589-020-0611-0}, issn = {1552-4469}, year = {2021}, date = {2021-01-01}, journal = {Nature Chemical Biology}, volume = {17}, number = {1}, pages = {30--38}, abstract = {Spectrally separated fluorophores allow the observation of multiple targets simultaneously inside living cells, leading to a deeper understanding of the molecular interplay that regulates cell function and fate. Chemogenetic systems combining a tag and a synthetic fluorophore provide certain advantages over fluorescent proteins since there is no requirement for chromophore maturation. Here, we present the engineering of a set of spectrally orthogonal fluorogen-activating tags based on the fluorescence-activating and absorption shifting tag (FAST) that are compatible with two-color, live-cell imaging. The resulting tags, greenFAST and redFAST, demonstrate orthogonality not only in their fluorogen recognition capabilities, but also in their one- and two-photon absorption profiles. This pair of orthogonal tags allowed the creation of a two-color cell cycle sensor capable of detecting very short, early cell cycles in zebrafish development and the development of split complementation systems capable of detecting multiple protein-protein interactions by live-cell fluorescence microscopy.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Spectrally separated fluorophores allow the observation of multiple targets simultaneously inside living cells, leading to a deeper understanding of the molecular interplay that regulates cell function and fate. Chemogenetic systems combining a tag and a synthetic fluorophore provide certain advantages over fluorescent proteins since there is no requirement for chromophore maturation. Here, we present the engineering of a set of spectrally orthogonal fluorogen-activating tags based on the fluorescence-activating and absorption shifting tag (FAST) that are compatible with two-color, live-cell imaging. The resulting tags, greenFAST and redFAST, demonstrate orthogonality not only in their fluorogen recognition capabilities, but also in their one- and two-photon absorption profiles. This pair of orthogonal tags allowed the creation of a two-color cell cycle sensor capable of detecting very short, early cell cycles in zebrafish development and the development of split complementation systems capable of detecting multiple protein-protein interactions by live-cell fluorescence microscopy. |
Recent Developments Concerning the Investigation of Exocytosis with Amperometry Article de journal M Guille-Collignon; F Lemaitre Current Opinion in Electrochemistry, 29 , p. 100751, 2021. @article{, title = {Recent Developments Concerning the Investigation of Exocytosis with Amperometry}, author = {M Guille-Collignon and F Lemaitre}, url = {https://doi.org/10.1016/j.coelec.2021.100751}, doi = {10.1016/j.coelec.2021.100751}, year = {2021}, date = {2021-01-01}, journal = {Current Opinion in Electrochemistry}, volume = {29}, pages = {100751}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Simulations of amperometric monitoring of exocytosis: moderate pH variations within the cell-electrode cleft with the buffer diffusion Article de journal Y Bouret; M Guille-Collignon; F Lemaitre Analytical and Bioanalytical Chemistry, 2021. @article{, title = {Simulations of amperometric monitoring of exocytosis: moderate pH variations within the cell-electrode cleft with the buffer diffusion}, author = {Y Bouret and M Guille-Collignon and F Lemaitre}, url = {https://doi.org/10.1007/s00216-021-03443-z}, doi = {10.1007/s00216-021-03443-z}, year = {2021}, date = {2021-01-01}, journal = {Analytical and Bioanalytical Chemistry}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ultrafast Dynamics of Fully Reduced Flavin in Catalytic Structures of Thymidylate Synthase ThyX Article de journal Nadia Dozova; Fabien Lacombat; Murielle Lombard; Djemel Hamdane; Pascal Plaza Physical Chemistry Chemical Physics, 23 , p. 22692-22702, 2021, ISSN: 1463-9076. @article{RN123b, title = {Ultrafast Dynamics of Fully Reduced Flavin in Catalytic Structures of Thymidylate Synthase ThyX}, author = {Nadia Dozova and Fabien Lacombat and Murielle Lombard and Djemel Hamdane and Pascal Plaza}, url = {http://dx.doi.org/10.1039/D1CP03379D}, doi = {10.1039/D1CP03379D}, issn = {1463-9076}, year = {2021}, date = {2021-01-01}, journal = {Physical Chemistry Chemical Physics}, volume = {23}, pages = {22692-22702}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ultrafast photoreduction dynamics of a new class of CPD photolyases Article de journal Fabien Lacombat; Agathe Espagne; Nadia Dozova; Pascal Plaza; Pavel Müller; Hans-Joachim Emmerich; Martin Saft; Lars-Oliver Essen Photochemical & Photobiological Sciences, 20 (6), p. 733-746, 2021, ISSN: 1474-9092. @article{RN632, title = {Ultrafast photoreduction dynamics of a new class of CPD photolyases}, author = {Fabien Lacombat and Agathe Espagne and Nadia Dozova and Pascal Plaza and Pavel M\"{u}ller and Hans-Joachim Emmerich and Martin Saft and Lars-Oliver Essen}, url = {https://doi.org/10.1007/s43630-021-00048-4}, doi = {10.1007/s43630-021-00048-4}, issn = {1474-9092}, year = {2021}, date = {2021-01-01}, journal = {Photochemical & Photobiological Sciences}, volume = {20}, number = {6}, pages = {733-746}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Arnaud Gautier; Ludovic Jullien; Chenge Li; Marie-Aude Plamont; Alison G Tebo; Marion Thauvin; Michel Volovitch; Sophie Vriz Springer US, New York, NY, 2021, ISBN: 978-1-07-161593-5. @book{gautier_versatile_2021, title = {Versatile On-Demand Fluorescent Labeling of Fusion Proteins Using Fluorescence-Activating and Absorption-Shifting Tag (FAST)}, author = {Arnaud Gautier and Ludovic Jullien and Chenge Li and Marie-Aude Plamont and Alison G Tebo and Marion Thauvin and Michel Volovitch and Sophie Vriz}, editor = {Eli Zamir}, url = {https://doi.org/10.1007/978-1-0716-1593-5_16}, doi = {10.1007/978-1-0716-1593-5_16}, isbn = {978-1-07-161593-5}, year = {2021}, date = {2021-01-01}, urldate = {2023-10-31}, booktitle = {Multiplexed Imaging: Methods and Protocols}, pages = {253--265}, publisher = {Springer US}, address = {New York, NY}, series = {Methods in Molecular Biology}, abstract = {Observing the localization, the concentration, and the distribution of proteins in cells or organisms is essential to understand theirs functions. General and versatile methods allowing multiplexed imaging of proteins under a large variety of experimental conditions are thus essential for deciphering the inner workings of cells and organisms. Here, we present a general method based on the non-covalent labeling of a small protein tag, named FAST (fluorescence-activating and absorption-shifting tag), with various fluorogenic ligands that light up upon labeling, which makes the simple, robust, and versatile on-demand labeling of fusion proteins in a wide range of experimental systems possible.}, keywords = {}, pubstate = {published}, tppubtype = {book} } Observing the localization, the concentration, and the distribution of proteins in cells or organisms is essential to understand theirs functions. General and versatile methods allowing multiplexed imaging of proteins under a large variety of experimental conditions are thus essential for deciphering the inner workings of cells and organisms. Here, we present a general method based on the non-covalent labeling of a small protein tag, named FAST (fluorescence-activating and absorption-shifting tag), with various fluorogenic ligands that light up upon labeling, which makes the simple, robust, and versatile on-demand labeling of fusion proteins in a wide range of experimental systems possible. |
2020 |
A Far-Red Emitting Fluorescent Chemogenetic Reporter for In Vivo Molecular Imaging Article de journal Chenge Li; Alison G Tebo; Marion Thauvin; Marie-Aude Plamont; Michel Volovitch; Xavier Morin; Sophie Vriz; Arnaud Gautier Angewandte Chemie International Edition, 59 (41), p. 17917–17923, 2020, ISSN: 1521-3773, (_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1002/anie.202006576). @article{li_far-red_2020, title = {A Far-Red Emitting Fluorescent Chemogenetic Reporter for In Vivo Molecular Imaging}, author = {Chenge Li and Alison G Tebo and Marion Thauvin and Marie-Aude Plamont and Michel Volovitch and Xavier Morin and Sophie Vriz and Arnaud Gautier}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.202006576}, doi = {10.1002/anie.202006576}, issn = {1521-3773}, year = {2020}, date = {2020-01-01}, urldate = {2023-10-31}, journal = {Angewandte Chemie International Edition}, volume = {59}, number = {41}, pages = {17917--17923}, abstract = {Far-red emitting fluorescent labels are highly desirable for spectral multiplexing and deep tissue imaging. Here, we describe the generation of frFAST (far-red Fluorescence Activating and absorption Shifting Tag), a 14-kDa monomeric protein that forms a bright far-red fluorescent assembly with (4-hydroxy-3-methoxy-phenyl)allylidene rhodanine (HPAR-3OM). As HPAR-3OM is essentially non-fluorescent in solution and in cells, frFAST can be imaged with high contrast in presence of free HPAR-3OM, which allowed the rapid and efficient imaging of frFAST fusions in live cells, zebrafish embryo/larvae, and chicken embryos. Beyond enabling the genetic encoding of far-red fluorescence, frFAST allowed the design of a far-red chemogenetic reporter of protein\textendashprotein interactions, demonstrating its great potential for the design of innovative far-red emitting biosensors.}, note = {_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1002/anie.202006576}, keywords = {}, pubstate = {published}, tppubtype = {article} } Far-red emitting fluorescent labels are highly desirable for spectral multiplexing and deep tissue imaging. Here, we describe the generation of frFAST (far-red Fluorescence Activating and absorption Shifting Tag), a 14-kDa monomeric protein that forms a bright far-red fluorescent assembly with (4-hydroxy-3-methoxy-phenyl)allylidene rhodanine (HPAR-3OM). As HPAR-3OM is essentially non-fluorescent in solution and in cells, frFAST can be imaged with high contrast in presence of free HPAR-3OM, which allowed the rapid and efficient imaging of frFAST fusions in live cells, zebrafish embryo/larvae, and chicken embryos. Beyond enabling the genetic encoding of far-red fluorescence, frFAST allowed the design of a far-red chemogenetic reporter of protein–protein interactions, demonstrating its great potential for the design of innovative far-red emitting biosensors. |
Disclosing the redox metabolism of drugs: The essential role of electrochemistry Article de journal O Buriez; E Labbe Current Opinion in Electrochemistry, 24 , p. 63-68, 2020. @article{, title = {Disclosing the redox metabolism of drugs: The essential role of electrochemistry}, author = {O Buriez and E Labbe}, url = {https://doi.org/10.1016/j.coelec.2020.07.002}, doi = {10.1016/j.coelec.2020.07.002}, year = {2020}, date = {2020-12-01}, journal = {Current Opinion in Electrochemistry}, volume = {24}, pages = {63-68}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Dynamic contrast for overcoming spectral interferences in fluorescence imaging Article de journal R Chouket; A Pellissier-Tanon; A Lemarchand; A Espagne; T Le Saux; L Jullien Journal of Physics-Photonics, 2 (3), p. 8, 2020, ISSN: 2515-7647. @article{RN1_30, title = {Dynamic contrast for overcoming spectral interferences in fluorescence imaging}, author = {R Chouket and A Pellissier-Tanon and A Lemarchand and A Espagne and T Le Saux and L Jullien}, url = {<Go to ISI>://WOS:000572940200001}, doi = {10.1088/2515-7647/ab9099}, issn = {2515-7647}, year = {2020}, date = {2020-01-01}, journal = {Journal of Physics-Photonics}, volume = {2}, number = {3}, pages = {8}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Dynamic contrast with reversibly photoswitchable fluorescent labels for imaging living cells Article de journal R Chouket; A Pellissier-Tanon; A Lemarchand; A Espagne; T Le Saux; L Jullien Chemical Science, 11 (11), p. 2882-2887, 2020, ISSN: 2041-6520. @article{RN2x, title = {Dynamic contrast with reversibly photoswitchable fluorescent labels for imaging living cells}, author = {R Chouket and A Pellissier-Tanon and A Lemarchand and A Espagne and T Le Saux and L Jullien}, url = {<Go to ISI>://WOS:000521247400002}, doi = {10.1039/d0sc00182a}, issn = {2041-6520}, year = {2020}, date = {2020-01-01}, journal = {Chemical Science}, volume = {11}, number = {11}, pages = {2882-2887}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Inducible intracellular membranes: molecular aspects and emerging applications Article de journal J Royes; V Biou; N Dautin; C Tribet; B Miroux Microbial Cell Factories, 19 (1), 2020. @article{RN263, title = {Inducible intracellular membranes: molecular aspects and emerging applications}, author = {J Royes and V Biou and N Dautin and C Tribet and B Miroux}, url = {<Go to ISI>://WOS:000568404600002}, doi = {10.1186/s12934-020-01433-x}, year = {2020}, date = {2020-01-01}, journal = {Microbial Cell Factories}, volume = {19}, number = {1}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Mediator-Microorganism Interaction in Microbial Solar Cell: a Fluo-Electrochemical Insight Article de journal L Beauzamy; J Delacotte; B Bailleul; K Tanaka; S Nakanishi; F A Wollman; F Lemaitre Analytical Chemistry, 92 (11), p. 7532-7539, 2020. @article{, title = {Mediator-Microorganism Interaction in Microbial Solar Cell: a Fluo-Electrochemical Insight}, author = {L Beauzamy and J Delacotte and B Bailleul and K Tanaka and S Nakanishi and F A Wollman and F Lemaitre}, url = {https://doi.org/10.1021/acs.analchem.9b05808}, doi = {10.1021/acs.analchem.9b05808}, year = {2020}, date = {2020-06-01}, journal = {Analytical Chemistry}, volume = {92}, number = {11}, pages = {7532-7539}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Overview and outlook of the strategies devoted to electrofluorescence surveys: Application to single cell secretion analysis Article de journal M Guille-Collignon; F Lemaitre Trac-Trends in Analytical Chemistry, 132 , p. 116055, 2020. @article{, title = {Overview and outlook of the strategies devoted to electrofluorescence surveys: Application to single cell secretion analysis}, author = {M Guille-Collignon and F Lemaitre}, url = {https://doi.org/10.1016/j.trac.2020.116055}, doi = {10.1016/j.trac.2020.116055}, year = {2020}, date = {2020-11-01}, journal = {Trac-Trends in Analytical Chemistry}, volume = {132}, pages = {116055}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Solubilization and Stabilization of Membrane Proteins by Cycloalkane-Modified Amphiphilic Polymers Article de journal Anaïs Marconnet; Baptiste Michon; Christel Le Bon; Fabrice Giusti; Christophe Tribet; Manuela Zoonens Biomacromolecules, 21 (8), p. 3459-3467, 2020, ISSN: 1525-7797. @article{Marconnet2020, title = {Solubilization and Stabilization of Membrane Proteins by Cycloalkane-Modified Amphiphilic Polymers}, author = {Ana\"{i}s Marconnet and Baptiste Michon and Christel Le Bon and Fabrice Giusti and Christophe Tribet and Manuela Zoonens }, editor = {American Chemical Society}, url = {https://doi.org/10.1021/acs.biomac.0c00929}, doi = {10.1021/acs.biomac.0c00929}, issn = {1525-7797}, year = {2020}, date = {2020-08-10}, journal = {Biomacromolecules}, volume = {21}, number = {8}, pages = {3459-3467}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
The strong potential of organic and molecular electrochemistry Article de journal O Buriez Current Opinion in Electrochemistry, 24 , p. A1-A3, 2020. @article{, title = {The strong potential of organic and molecular electrochemistry}, author = {O Buriez}, url = {https://doi.org/10.1016/j.coelec.2020.100657}, doi = {10.1016/j.coelec.2020.100657}, year = {2020}, date = {2020-12-01}, journal = {Current Opinion in Electrochemistry}, volume = {24}, pages = {A1-A3}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Underlying mechanisms in microbial solar cells: how modeling can help Article de journal L Beauzamy; F Lemaitre; J Derr Sustainable Energy & Fuels, 4 (12), p. 6004-6010, 2020. @article{, title = {Underlying mechanisms in microbial solar cells: how modeling can help}, author = {L Beauzamy and F Lemaitre and J Derr}, url = {https://doi.org/10.1039/d0se01304h}, doi = {10.1039/d0se01304h}, year = {2020}, date = {2020-12-01}, journal = {Sustainable Energy & Fuels}, volume = {4}, number = {12}, pages = {6004-6010}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2019 |
A Fluorescent False Neurotransmitter as a Dual Electrofluorescent Probe for Secretory Cell Models Article de journal J Pandard; N Pan; D H Ebene; T Le Saux; E Ait-Yahiatène; X Liu; L Grimaud; O Buriez; E Labbé; F Lemaître; M Guille-Collignon ChemPlusChem, 84 (10), p. 1578-1586, 2019, (cited By 0). @article{Pandard20191578, title = {A Fluorescent False Neurotransmitter as a Dual Electrofluorescent Probe for Secretory Cell Models}, author = {J Pandard and N Pan and D H Ebene and T Le Saux and E Ait-Yahiat\`{e}ne and X Liu and L Grimaud and O Buriez and E Labb\'{e} and F Lema\^{i}tre and M Guille-Collignon}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85073931778&doi=10.1002%2fcplu.201900385&partnerID=40&md5=a526291d966e7dc926b44e97c73794b3}, doi = {10.1002/cplu.201900385}, year = {2019}, date = {2019-01-01}, journal = {ChemPlusChem}, volume = {84}, number = {10}, pages = {1578-1586}, note = {cited By 0}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
A split fluorescent reporter with rapid and reversible complementation Article de journal Alison G Tebo; Arnaud Gautier Nature Communications, 10 (1), p. 2822, 2019, ISSN: 2041-1723. @article{Tebo2019, title = {A split fluorescent reporter with rapid and reversible complementation}, author = {Alison G Tebo and Arnaud Gautier}, url = {https://doi.org/10.1038/s41467-019-10855-0}, doi = {10.1038/s41467-019-10855-0}, issn = {2041-1723}, year = {2019}, date = {2019-01-01}, journal = {Nature Communications}, volume = {10}, number = {1}, pages = {2822}, abstract = {Interactions between proteins play an essential role in metabolic and signaling pathways, cellular processes and organismal systems. We report the development of splitFAST, a fluorescence complementation system for the visualization of transient protein-protein interactions in living cells. Engineered from the fluorogenic reporter FAST (Fluorescence-Activating and absorption-Shifting Tag), which specifically and reversibly binds fluorogenic hydroxybenzylidene rhodanine (HBR) analogs, splitFAST displays rapid and reversible complementation, allowing the real-time visualization of both the formation and the dissociation of a protein assembly.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Interactions between proteins play an essential role in metabolic and signaling pathways, cellular processes and organismal systems. We report the development of splitFAST, a fluorescence complementation system for the visualization of transient protein-protein interactions in living cells. Engineered from the fluorogenic reporter FAST (Fluorescence-Activating and absorption-Shifting Tag), which specifically and reversibly binds fluorogenic hydroxybenzylidene rhodanine (HBR) analogs, splitFAST displays rapid and reversible complementation, allowing the real-time visualization of both the formation and the dissociation of a protein assembly. |
Bacteria-Based Production of Thiol-Clickable, Genetically Encoded Lipid Nanovesicles Article de journal Jorge Royes; Oana Ilioaia; Quentin Lubart; Federica Angius; Galina V Dubacheva; Marta Bally; Bruno Miroux; Christophe Tribet Angewandte Chemie International Edition, 58 , p. 7395-7399, 2019, ISSN: 1433-7851. @article{RN1x, title = {Bacteria-Based Production of Thiol-Clickable, Genetically Encoded Lipid Nanovesicles}, author = {Jorge Royes and Oana Ilioaia and Quentin Lubart and Federica Angius and Galina V Dubacheva and Marta Bally and Bruno Miroux and Christophe Tribet}, doi = {10.1002/anie.201902929}, issn = {1433-7851}, year = {2019}, date = {2019-01-01}, journal = {Angewandte Chemie International Edition}, volume = {58}, pages = {7395-7399}, keywords = {}, pubstate = {published}, tppubtype = {article} } |