You will find below the publication list of our pole.
For the publication list of each pole member, please see his/her personal webpage.
2025 |
A tunable and versatile chemogenetic near-infrared fluorescent reporter Article de journal Lina El Hajji; Benjamin Bunel; Octave Joliot; Chenge Li; Alison G Tebo; Christine Rampon; Michel Volovitch; Evelyne Fischer; Nicolas Pietrancosta; Franck Perez; Xavier Morin; Sophie Vriz; Arnaud Gautier Nature Communications, 16 (1), p. 2594, 2025, ISSN: 2041-1723. @article{el_hajji_tunable_2025, title = {A tunable and versatile chemogenetic near-infrared fluorescent reporter}, author = {Lina El Hajji and Benjamin Bunel and Octave Joliot and Chenge Li and Alison G Tebo and Christine Rampon and Michel Volovitch and Evelyne Fischer and Nicolas Pietrancosta and Franck Perez and Xavier Morin and Sophie Vriz and Arnaud Gautier}, url = {https://doi.org/10.1038/s41467-025-58017-9}, doi = {10.1038/s41467-025-58017-9}, issn = {2041-1723}, year = {2025}, date = {2025-01-01}, journal = {Nature Communications}, volume = {16}, number = {1}, pages = {2594}, abstract = {Near-infrared (NIR) fluorescent reporters open interesting perspectives for multiplexed imaging with higher contrast and depth using less toxic light. Here, we propose nirFAST, a small (14 kDa) chemogenetic NIR fluorescent reporter, displaying higher cellular brightness compared to top-performing NIR fluorescent proteins. nirFAST binds and stabilizes the fluorescent state of synthetic cell permeant fluorogenic chromophores (so-called fluorogens), otherwise dark when free. nirFAST displays tunable NIR, far-red or red emission through change of fluorogen. nirFAST allows imaging and spectral multiplexing in live cultured mammalian cells, chicken embryo tissues and zebrafish larvae. Its suitability for stimulated emission depletion nanoscopy enabled protein imaging with subdiffraction resolution in live cells. nirFAST enabled the design of a two-color cell cycle indicator for monitoring the different phases of the cell cycle. Finally, bisection of nirFAST allowed the design of a chemically induced dimerization technology with NIR fluorescence readout, enabling the control and visualization of protein proximity.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Near-infrared (NIR) fluorescent reporters open interesting perspectives for multiplexed imaging with higher contrast and depth using less toxic light. Here, we propose nirFAST, a small (14 kDa) chemogenetic NIR fluorescent reporter, displaying higher cellular brightness compared to top-performing NIR fluorescent proteins. nirFAST binds and stabilizes the fluorescent state of synthetic cell permeant fluorogenic chromophores (so-called fluorogens), otherwise dark when free. nirFAST displays tunable NIR, far-red or red emission through change of fluorogen. nirFAST allows imaging and spectral multiplexing in live cultured mammalian cells, chicken embryo tissues and zebrafish larvae. Its suitability for stimulated emission depletion nanoscopy enabled protein imaging with subdiffraction resolution in live cells. nirFAST enabled the design of a two-color cell cycle indicator for monitoring the different phases of the cell cycle. Finally, bisection of nirFAST allowed the design of a chemically induced dimerization technology with NIR fluorescence readout, enabling the control and visualization of protein proximity. |
2024 |
Electrochemical Behavior of Quinones Classically Used for Bioenergetical Applications: Considerations and Insights about the Anodic Side Article de journal Guillaume Longatte; Olivier Buriez; Eric Labbé; Manon Guille-Collignon; Frédéric Lema^itre ChemElectroChem, 11 (5), p. e202300542, 2024. @article{longatte2024electrochemical, title = {Electrochemical Behavior of Quinones Classically Used for Bioenergetical Applications: Considerations and Insights about the Anodic Side}, author = {Guillaume Longatte and Olivier Buriez and Eric Labb\'{e} and Manon Guille-Collignon and Fr\'{e}d\'{e}ric Lema{^i}tre}, year = {2024}, date = {2024-01-01}, journal = {ChemElectroChem}, volume = {11}, number = {5}, pages = {e202300542}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Electrochemiluminescent imaging of a NADH-based enzymatic reaction confined within giant liposomes Article de journal Fatma Ben Trad; Bixente Carré; Jér^ome Delacotte; Frédéric Lema^itre; Manon Guille-Collignon; Stéphane Arbault; Neso Sojic; Eric Labbé; Olivier Buriez Analytical and Bioanalytical Chemistry, p. 1–10, 2024. @article{ben2024electrochemiluminescent, title = {Electrochemiluminescent imaging of a NADH-based enzymatic reaction confined within giant liposomes}, author = {Fatma Ben Trad and Bixente Carr\'{e} and J\'{e}r{^o}me Delacotte and Fr\'{e}d\'{e}ric Lema{^i}tre and Manon Guille-Collignon and St\'{e}phane Arbault and Neso Sojic and Eric Labb\'{e} and Olivier Buriez}, year = {2024}, date = {2024-01-01}, journal = {Analytical and Bioanalytical Chemistry}, pages = {1--10}, publisher = {Springer Berlin Heidelberg}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Interplay Between Molecular Electrochemistry and Light in Mechanistic Investigations Article de journal Eric Labbé; Olivier Buriez Current Opinion in Electrochemistry, p. 101613, 2024. @article{labbe2024interplay, title = {Interplay Between Molecular Electrochemistry and Light in Mechanistic Investigations}, author = {Eric Labb\'{e} and Olivier Buriez}, year = {2024}, date = {2024-01-01}, journal = {Current Opinion in Electrochemistry}, pages = {101613}, publisher = {Elsevier}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Multiplexed In Vivo Imaging with Fluorescence Lifetime-Modulating Tags Article de journal Lina El Hajji; France Lam; Maria Avtodeeva; Hela Benaissa; Christine Rampon; Michel Volovitch; Sophie Vriz; Arnaud Gautier Advanced Science, 11 (32), p. 2404354, 2024. @article{https://doi.org/10.1002/advs.202404354, title = {Multiplexed In Vivo Imaging with Fluorescence Lifetime-Modulating Tags}, author = {Lina El Hajji and France Lam and Maria Avtodeeva and Hela Benaissa and Christine Rampon and Michel Volovitch and Sophie Vriz and Arnaud Gautier}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202404354}, doi = {https://doi.org/10.1002/advs.202404354}, year = {2024}, date = {2024-01-01}, journal = {Advanced Science}, volume = {11}, number = {32}, pages = {2404354}, abstract = {Abstract Fluorescence lifetime imaging microscopy (FLIM) opens new dimensions for highly multiplexed imaging in live cells and organisms using differences in fluorescence lifetime to distinguish spectrally identical fluorescent probes. Here, a set of fluorescence-activating and absorption-shifting tags (FASTs) capable of modulating the fluorescence lifetime of embedded fluorogenic 4-hydroxybenzylidene rhodanine (HBR) derivatives is described. It is shown that changes in the FAST protein sequence can vary the local environment of the chromophore and lead to significant changes in fluorescence lifetime. These fluorescence lifetime-modulating tags enable multiplexed imaging of up to three targets in one spectral channel using a single HBR derivative in live cells and live zebrafish larvae. The combination of fluorescence lifetime multiplexing with spectral multiplexing allows to successfully image six targets in live cells, opening great prospects for multicolor fluorescence lifetime multiplexing.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Fluorescence lifetime imaging microscopy (FLIM) opens new dimensions for highly multiplexed imaging in live cells and organisms using differences in fluorescence lifetime to distinguish spectrally identical fluorescent probes. Here, a set of fluorescence-activating and absorption-shifting tags (FASTs) capable of modulating the fluorescence lifetime of embedded fluorogenic 4-hydroxybenzylidene rhodanine (HBR) derivatives is described. It is shown that changes in the FAST protein sequence can vary the local environment of the chromophore and lead to significant changes in fluorescence lifetime. These fluorescence lifetime-modulating tags enable multiplexed imaging of up to three targets in one spectral channel using a single HBR derivative in live cells and live zebrafish larvae. The combination of fluorescence lifetime multiplexing with spectral multiplexing allows to successfully image six targets in live cells, opening great prospects for multicolor fluorescence lifetime multiplexing. |
Multivalent interactions of the disordered regions of XLF and XRCC4 foster robust cellular NHEJ and drive the formation of ligation-boosting condensates in vitro Article de journal Duc-Duy Vu; Alessio Bonucci; Manon Brenière; Metztli Cisneros-Aguirre; Philippe Pelupessy; Ziqing Wang; Ludovic Carlier; Guillaume Bouvignies; Patricia Cortes; Aneel K Aggarwal; Martin Blackledge; Zoher Gueroui; Valérie Belle; Jeremy M Stark; Mauro Modesti; Fabien Ferrage Nature Structural & Molecular Biology, 31 (11), p. 1732–1744, 2024, ISSN: 1545-9985. @article{vu_multivalent_2024, title = {Multivalent interactions of the disordered regions of XLF and XRCC4 foster robust cellular NHEJ and drive the formation of ligation-boosting condensates in vitro}, author = {Duc-Duy Vu and Alessio Bonucci and Manon Breni\`{e}re and Metztli Cisneros-Aguirre and Philippe Pelupessy and Ziqing Wang and Ludovic Carlier and Guillaume Bouvignies and Patricia Cortes and Aneel K Aggarwal and Martin Blackledge and Zoher Gueroui and Val\'{e}rie Belle and Jeremy M Stark and Mauro Modesti and Fabien Ferrage}, url = {https://doi.org/10.1038/s41594-024-01339-x}, doi = {10.1038/s41594-024-01339-x}, issn = {1545-9985}, year = {2024}, date = {2024-01-01}, journal = {Nature Structural & Molecular Biology}, volume = {31}, number = {11}, pages = {1732--1744}, abstract = {In mammalian cells, DNA double-strand breaks are predominantly repaired by non-homologous end joining (NHEJ). During repair, the Ku70\textendashKu80 heterodimer (Ku), X-ray repair cross complementing 4 (XRCC4) in complex with DNA ligase 4 (X4L4) and XRCC4-like factor (XLF) form a flexible scaffold that holds the broken DNA ends together. Insights into the architectural organization of the NHEJ scaffold and its regulation by the DNA-dependent protein kinase catalytic subunit (DNA-PKcs) were recently obtained by single-particle cryo-electron microscopy analysis. However, several regions, especially the C-terminal regions (CTRs) of the XRCC4 and XLF scaffolding proteins, have largely remained unresolved in experimental structures, which hampers the understanding of their functions. Here we used magnetic resonance techniques and biochemical assays to comprehensively characterize the interactions and dynamics of the XRCC4 and XLF CTRs at residue resolution. We show that the CTRs of XRCC4 and XLF are intrinsically disordered and form a network of multivalent heterotypic and homotypic interactions that promotes robust cellular NHEJ activity. Importantly, we demonstrate that the multivalent interactions of these CTRs lead to the formation of XLF and X4L4 condensates in vitro, which can recruit relevant effectors and critically stimulate DNA end ligation. Our work highlights the role of disordered regions in the mechanism and dynamics of NHEJ and lays the groundwork for the investigation of NHEJ protein disorder and its associated condensates inside cells with implications in cancer biology, immunology and the development of genome-editing strategies.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In mammalian cells, DNA double-strand breaks are predominantly repaired by non-homologous end joining (NHEJ). During repair, the Ku70–Ku80 heterodimer (Ku), X-ray repair cross complementing 4 (XRCC4) in complex with DNA ligase 4 (X4L4) and XRCC4-like factor (XLF) form a flexible scaffold that holds the broken DNA ends together. Insights into the architectural organization of the NHEJ scaffold and its regulation by the DNA-dependent protein kinase catalytic subunit (DNA-PKcs) were recently obtained by single-particle cryo-electron microscopy analysis. However, several regions, especially the C-terminal regions (CTRs) of the XRCC4 and XLF scaffolding proteins, have largely remained unresolved in experimental structures, which hampers the understanding of their functions. Here we used magnetic resonance techniques and biochemical assays to comprehensively characterize the interactions and dynamics of the XRCC4 and XLF CTRs at residue resolution. We show that the CTRs of XRCC4 and XLF are intrinsically disordered and form a network of multivalent heterotypic and homotypic interactions that promotes robust cellular NHEJ activity. Importantly, we demonstrate that the multivalent interactions of these CTRs lead to the formation of XLF and X4L4 condensates in vitro, which can recruit relevant effectors and critically stimulate DNA end ligation. Our work highlights the role of disordered regions in the mechanism and dynamics of NHEJ and lays the groundwork for the investigation of NHEJ protein disorder and its associated condensates inside cells with implications in cancer biology, immunology and the development of genome-editing strategies. |
Shadow electrochemiluminescence imaging of giant liposomes opening at polarized electrodes Article de journal Fatma Ben Trad; Jér^ome Delacotte; Frédéric Lema^itre; Manon Guille-Collignon; Stéphane Arbault; Neso Sojic; Eric Labbé; Olivier Buriez Analyst, 2024. @article{trad2024shadow, title = {Shadow electrochemiluminescence imaging of giant liposomes opening at polarized electrodes}, author = {Fatma Ben Trad and J\'{e}r{^o}me Delacotte and Fr\'{e}d\'{e}ric Lema{^i}tre and Manon Guille-Collignon and St\'{e}phane Arbault and Neso Sojic and Eric Labb\'{e} and Olivier Buriez}, year = {2024}, date = {2024-01-01}, journal = {Analyst}, publisher = {Royal Society of Chemistry}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Small-molecule autocatalysis drives compartment growth, competition and reproduction Article de journal Heng Lu; Alex Blokhuis; Rebecca Turk-MacLeod; Jayaprakash Karuppusamy; Andrea Franconi; Gabrielle Woronoff; Cyrille Jeancolas; Afshin Abrishamkar; Estelle Loire; Fabien Ferrage; Philippe Pelupessy; Ludovic Jullien; Eörs Szathmary; Philippe Nghe; Andrew D Griffiths Nature Chemistry, 16 , p. 70-78, 2024, ISSN: 1755-4330, 1755-4349. @article{lu_small-molecule_2023, title = {Small-molecule autocatalysis drives compartment growth, competition and reproduction}, author = {Heng Lu and Alex Blokhuis and Rebecca Turk-MacLeod and Jayaprakash Karuppusamy and Andrea Franconi and Gabrielle Woronoff and Cyrille Jeancolas and Afshin Abrishamkar and Estelle Loire and Fabien Ferrage and Philippe Pelupessy and Ludovic Jullien and E\"{o}rs Szathmary and Philippe Nghe and Andrew D Griffiths}, url = {https://www.nature.com/articles/s41557-023-01276-0}, doi = {10.1038/s41557-023-01276-0}, issn = {1755-4330, 1755-4349}, year = {2024}, date = {2024-01-01}, urldate = {2023-12-18}, journal = {Nature Chemistry}, volume = {16}, pages = {70-78}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2023 |
Electrochemiluminescence imaging of liposome permeabilization by an antimicrobial peptide: melittin Article de journal Fatma Ben Trad; Jér^ome Delacotte; Manon Guille-Collignon; Frédéric Lema^itre; Stéphane Arbault; Neso Sojic; Fabienne Burlina; Eric Labbé; Olivier Buriez Chemical & Biomedical Imaging, 1 (1), p. 58–65, 2023. @article{ben2023electrochemiluminescence, title = {Electrochemiluminescence imaging of liposome permeabilization by an antimicrobial peptide: melittin}, author = {Fatma Ben Trad and J\'{e}r{^o}me Delacotte and Manon Guille-Collignon and Fr\'{e}d\'{e}ric Lema{^i}tre and St\'{e}phane Arbault and Neso Sojic and Fabienne Burlina and Eric Labb\'{e} and Olivier Buriez}, year = {2023}, date = {2023-01-01}, journal = {Chemical & Biomedical Imaging}, volume = {1}, number = {1}, pages = {58--65}, publisher = {Nanjing University and American Chemical Society}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Monitoring and Assessing Iridium-Promoted Photoredox Catalysis by Electrochemistry Article de journal Julie Quintaine; Lionel Saudan; Francesco Santoro; Gilles Oddon; Eric Labbé; Olivier Buriez ACS Catalysis, 13 (22), p. 14894–14906, 2023. @article{quintaine2023monitoring, title = {Monitoring and Assessing Iridium-Promoted Photoredox Catalysis by Electrochemistry}, author = {Julie Quintaine and Lionel Saudan and Francesco Santoro and Gilles Oddon and Eric Labb\'{e} and Olivier Buriez}, year = {2023}, date = {2023-01-01}, journal = {ACS Catalysis}, volume = {13}, number = {22}, pages = {14894--14906}, publisher = {American Chemical Society}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Multiscale Transient Absorption Study of the Fluorescent Protein Dreiklang and Two Point Variants Provides Insight into Photoswitching and Nonproductive Reaction Pathways Article de journal Emilie Renouard; Magdalena Nowinska; Fabien Lacombat; Pascal Plaza; Pavel Müller; Agathe Espagne The Journal of Physical Chemistry Letters, 14 (28), p. 6477-6485, 2023. @article{RN63c, title = {Multiscale Transient Absorption Study of the Fluorescent Protein Dreiklang and Two Point Variants Provides Insight into Photoswitching and Nonproductive Reaction Pathways}, author = {Emilie Renouard and Magdalena Nowinska and Fabien Lacombat and Pascal Plaza and Pavel M\"{u}ller and Agathe Espagne}, url = {https://doi.org/10.1021/acs.jpclett.3c00431}, doi = {10.1021/acs.jpclett.3c00431}, year = {2023}, date = {2023-01-01}, journal = {The Journal of Physical Chemistry Letters}, volume = {14}, number = {28}, pages = {6477-6485}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Near-infrared co-illumination of fluorescent proteins reduces photobleaching and phototoxicity Article de journal L Ludvikova; E Simon; M Deygas; T Panier; M A Plamont; J Ollion; A Tebo; M Piel; L Jullien; L Robert; T Le Saux; A Espagne Nature Biotechnology, p. 12, 2023, ISSN: 1087-0156. @article{RN156, title = {Near-infrared co-illumination of fluorescent proteins reduces photobleaching and phototoxicity}, author = {L Ludvikova and E Simon and M Deygas and T Panier and M A Plamont and J Ollion and A Tebo and M Piel and L Jullien and L Robert and T Le Saux and A Espagne}, url = {<Go to ISI>://WOS:001042699900001}, doi = {10.1038/s41587-023-01893-7}, issn = {1087-0156}, year = {2023}, date = {2023-01-01}, journal = {Nature Biotechnology}, pages = {12}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2022 |
An expanded palette of fluorogenic HaloTag probes with enhanced contrast for targeted cellular imaging Article de journal Sylvestre P J T Bachollet; Yuriy Shpinov; Fanny Broch; Hela Benaissa; Arnaud Gautier; Nicolas Pietrancosta; Jean-Maurice Mallet; Blaise Dumat Organic & Biomolecular Chemistry, 20 (17), p. 3619 - 3628, 2022, ISSN: 1477-0520. @article{Bachollet2022, title = {An expanded palette of fluorogenic HaloTag probes with enhanced contrast for targeted cellular imaging}, author = {Sylvestre P J T Bachollet and Yuriy Shpinov and Fanny Broch and Hela Benaissa and Arnaud Gautier and Nicolas Pietrancosta and Jean-Maurice Mallet and Blaise Dumat}, url = {http://xlink.rsc.org/?DOI=D1OB02394B}, doi = {10.1039/D1OB02394B}, issn = {1477-0520}, year = {2022}, date = {2022-01-01}, journal = {Organic & Biomolecular Chemistry}, volume = {20}, number = {17}, pages = {3619 - 3628}, publisher = {Royal Society of Chemistry}, abstract = {A palette of fluorogenic molecular rotor probes with emissions from green to NIR was developed for wash-free and multicolor imaging of genetically-encoded HaloTag fusion proteins.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A palette of fluorogenic molecular rotor probes with emissions from green to NIR was developed for wash-free and multicolor imaging of genetically-encoded HaloTag fusion proteins. |
Dynamic electrochemiluminescence imaging of single giant liposome opening at polarized electrodes Article de journal Fatma Ben Trad; Vincent Wieczny; Jér^ome Delacotte; Mathieu Morel; Manon Guille-Collignon; Stéphane Arbault; Frédéric Lema^itre; Neso Sojic; Eric Labbé; Olivier Buriez Analytical Chemistry, 94 (3), p. 1686–1696, 2022. @article{ben2022dynamic, title = {Dynamic electrochemiluminescence imaging of single giant liposome opening at polarized electrodes}, author = {Fatma Ben Trad and Vincent Wieczny and J\'{e}r{^o}me Delacotte and Mathieu Morel and Manon Guille-Collignon and St\'{e}phane Arbault and Fr\'{e}d\'{e}ric Lema{^i}tre and Neso Sojic and Eric Labb\'{e} and Olivier Buriez}, year = {2022}, date = {2022-01-01}, journal = {Analytical Chemistry}, volume = {94}, number = {3}, pages = {1686--1696}, publisher = {American Chemical Society}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Electrode-supported and free-standing bilayer lipid membranes: Formation and uses in molecular electrochemistry Article de journal Eric Labbé; Olivier Buriez Electrochemical Science Advances, 2 (6), p. e2100170, 2022. @article{labbe2022electrode, title = {Electrode-supported and free-standing bilayer lipid membranes: Formation and uses in molecular electrochemistry}, author = {Eric Labb\'{e} and Olivier Buriez}, year = {2022}, date = {2022-01-01}, journal = {Electrochemical Science Advances}, volume = {2}, number = {6}, pages = {e2100170}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Reciprocal Regulation of Shh Trafficking and H2O2 Levels via a Noncanonical BOC-Rac1 Pathway Article de journal Marion Thauvin; Irène Amblard; Christine Rampon; Aurélien Mourton; Isabelle Queguiner; Chenge Li; Arnaud Gautier; Alain Joliot; Michel Volovitch; Sophie Vriz Antioxidants (Basel, Switzerland), 11 (4), p. 718, 2022, ISSN: 2076-3921. @article{thauvin_reciprocal_2022, title = {Reciprocal Regulation of Shh Trafficking and H2O2 Levels via a Noncanonical BOC-Rac1 Pathway}, author = {Marion Thauvin and Ir\`{e}ne Amblard and Christine Rampon and Aur\'{e}lien Mourton and Isabelle Queguiner and Chenge Li and Arnaud Gautier and Alain Joliot and Michel Volovitch and Sophie Vriz}, doi = {10.3390/antiox11040718}, issn = {2076-3921}, year = {2022}, date = {2022-01-01}, journal = {Antioxidants (Basel, Switzerland)}, volume = {11}, number = {4}, pages = {718}, abstract = {Among molecules that bridge environment, cell metabolism, and cell signaling, hydrogen peroxide (H2O2) recently appeared as an emerging but central player. Its level depends on cell metabolism and environment and was recently shown to play key roles during embryogenesis, contrasting with its long-established role in disease progression. We decided to explore whether the secreted morphogen Sonic hedgehog (Shh), known to be essential in a variety of biological processes ranging from embryonic development to adult tissue homeostasis and cancers, was part of these interactions. Here, we report that H2O2 levels control key steps of Shh delivery in cell culture: increased levels reduce primary secretion, stimulate endocytosis and accelerate delivery to recipient cells; in addition, physiological in vivo modulation of H2O2 levels changes Shh distribution and tissue patterning. Moreover, a feedback loop exists in which Shh trafficking controls H2O2 synthesis via a non-canonical BOC-Rac1 pathway, leading to cytoneme growth. Our findings reveal that Shh directly impacts its own distribution, thus providing a molecular explanation for the robustness of morphogenesis to both environmental insults and individual variability.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Among molecules that bridge environment, cell metabolism, and cell signaling, hydrogen peroxide (H2O2) recently appeared as an emerging but central player. Its level depends on cell metabolism and environment and was recently shown to play key roles during embryogenesis, contrasting with its long-established role in disease progression. We decided to explore whether the secreted morphogen Sonic hedgehog (Shh), known to be essential in a variety of biological processes ranging from embryonic development to adult tissue homeostasis and cancers, was part of these interactions. Here, we report that H2O2 levels control key steps of Shh delivery in cell culture: increased levels reduce primary secretion, stimulate endocytosis and accelerate delivery to recipient cells; in addition, physiological in vivo modulation of H2O2 levels changes Shh distribution and tissue patterning. Moreover, a feedback loop exists in which Shh trafficking controls H2O2 synthesis via a non-canonical BOC-Rac1 pathway, leading to cytoneme growth. Our findings reveal that Shh directly impacts its own distribution, thus providing a molecular explanation for the robustness of morphogenesis to both environmental insults and individual variability. |
Synthesis, Electrochemical and Fluorescence Properties of the First Fluorescent Member of the Ferrocifen Family and of Its Oxidized Derivatives Article de journal Charles Fayolle; Pascal Pigeon; Nathalie Fischer-Durand; Mich`ele Salmain; Olivier Buriez; Anne Vessi`eres; Eric Labbé Molecules, 27 (19), p. 6690, 2022. @article{fayolle2022synthesis, title = {Synthesis, Electrochemical and Fluorescence Properties of the First Fluorescent Member of the Ferrocifen Family and of Its Oxidized Derivatives}, author = {Charles Fayolle and Pascal Pigeon and Nathalie Fischer-Durand and Mich{`e}le Salmain and Olivier Buriez and Anne Vessi{`e}res and Eric Labb\'{e}}, year = {2022}, date = {2022-01-01}, journal = {Molecules}, volume = {27}, number = {19}, pages = {6690}, publisher = {MDPI}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Transition kinetics of mixed lipid:photosurfactant assemblies studied by time-resolved small angle X-ray scattering Article de journal Jorges Royes; V. A. Bjørnestad; G. Brun; Th. Narayanan; R Lund; C Tribet J Colloid Interface Sci., 610 , p. 830-841, 2022, ISBN: 0021-9797. @article{Royes2022, title = {Transition kinetics of mixed lipid:photosurfactant assemblies studied by time-resolved small angle X-ray scattering }, author = {Jorges Royes and V. A. Bj\ornestad and G. Brun and Th. Narayanan and R Lund and C Tribet}, editor = {Elsevier}, url = {https://www.sciencedirect.com/science/article/abs/pii/S0021979721020439?via%3Dihub}, doi = {10.1016/j.jcis.2021.11.133 }, isbn = {0021-9797}, year = {2022}, date = {2022-03-15}, journal = {J Colloid Interface Sci.}, volume = {610}, pages = {830-841}, abstract = {Hypothesis: Photoswitchable surfactants are used in the design of many light-responsive colloids and/or self-assemblies. Photo-isomerization enables to control molecular equilibrium, and triggers transient reorganizations with possibly out-of-equilibrium intermediate states that have been overlooked. Here, we address this question by an in depth structural investigation of intermediate lipid-surfactant assemblies that occur during fast isothermal photo-triggered transition in lipid:surfactant mixtures. Experiments: The structural parameters of mixed assemblies of azobenzene-containing cationic surfactant (AzoTMA) and dioleoylphosphatidylcholine (DOPC) lipids were studied by light scattering and time-resolved small angle X-ray scattering. Structural and compositional information about the assemblies and unimers in bulk were determined at the photostationary states, as well as at intermediate kinetic states formed during UV or blue light illumination. Findings: DOPC:AzoTMA systems form mixed assemblies representative of phospholipid:cationic surfactant mixtures, that evolve from spheroid, to rod-like micelles, and vesicles with increasing DOPC fraction. Transient assemblies detected during the photo-triggered kinetics are similar to the ones found in stationary states. But changes of AzoTMA unimers in bulk can be considerably faster than mass reorganizations of the mixed assemblies, suggesting that out-of-equilibrium conditions are transiently reached. Mass reorganization of the surfactant-enriched assemblies is much faster than in the lipid enriched ones, providing insight into the role of lipids in a slow reorganization of the assemblies. }, keywords = {}, pubstate = {published}, tppubtype = {article} } Hypothesis: Photoswitchable surfactants are used in the design of many light-responsive colloids and/or self-assemblies. Photo-isomerization enables to control molecular equilibrium, and triggers transient reorganizations with possibly out-of-equilibrium intermediate states that have been overlooked. Here, we address this question by an in depth structural investigation of intermediate lipid-surfactant assemblies that occur during fast isothermal photo-triggered transition in lipid:surfactant mixtures. Experiments: The structural parameters of mixed assemblies of azobenzene-containing cationic surfactant (AzoTMA) and dioleoylphosphatidylcholine (DOPC) lipids were studied by light scattering and time-resolved small angle X-ray scattering. Structural and compositional information about the assemblies and unimers in bulk were determined at the photostationary states, as well as at intermediate kinetic states formed during UV or blue light illumination. Findings: DOPC:AzoTMA systems form mixed assemblies representative of phospholipid:cationic surfactant mixtures, that evolve from spheroid, to rod-like micelles, and vesicles with increasing DOPC fraction. Transient assemblies detected during the photo-triggered kinetics are similar to the ones found in stationary states. But changes of AzoTMA unimers in bulk can be considerably faster than mass reorganizations of the mixed assemblies, suggesting that out-of-equilibrium conditions are transiently reached. Mass reorganization of the surfactant-enriched assemblies is much faster than in the lipid enriched ones, providing insight into the role of lipids in a slow reorganization of the assemblies. |
UCST-Type Polymer Capsules Formed by Interfacial Complexation Article de journal L Sixdenier; A Auge; Y Zhao; E Marie; C Tribet ACS MACRO LETTERS, 11 (5), p. 651-656, 2022, ISBN: 2161-1653 J9 - ACS MACRO LETT. @article{Sixdenier2022, title = {UCST-Type Polymer Capsules Formed by Interfacial Complexation}, author = {L Sixdenier and A Auge and Y Zhao and E Marie and C Tribet}, doi = {10.1021/acsmacrolett.2c00021}, isbn = {2161-1653 J9 - ACS MACRO LETT}, year = {2022}, date = {2022-05-17}, journal = {ACS MACRO LETTERS}, volume = {11}, number = {5}, pages = {651-656}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2021 |
An Electrochemical Study of Bis(cyclopentadienyl)titanium(IV) Dichloride in the Presence of Magnesium Ions, Amides or Alkynes Article de journal A K D Dime; Y Six; O Buriez Russian Journal of Electrochemistry, 57 (1), p. 85-91, 2021. @article{, title = {An Electrochemical Study of Bis(cyclopentadienyl)titanium(IV) Dichloride in the Presence of Magnesium Ions, Amides or Alkynes}, author = {A K D Dime and Y Six and O Buriez}, url = {https://doi.org/10.1134/s1023193521010031}, doi = {10.1134/s1023193521010031}, year = {2021}, date = {2021-01-01}, journal = {Russian Journal of Electrochemistry}, volume = {57}, number = {1}, pages = {85-91}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Electrochemical Fluorescence Switch of Organic Fluorescent or Fluorogenic Molecules Article de journal M Guille-Collignon; J M Delacotte; F Lemaitre; E Labbe; O Buriez Chemical Record, 21 (9), p. 2193-2202, 2021. @article{, title = {Electrochemical Fluorescence Switch of Organic Fluorescent or Fluorogenic Molecules}, author = {M Guille-Collignon and J M Delacotte and F Lemaitre and E Labbe and O Buriez}, url = {https://doi.org/10.1002/tcr.202100022}, doi = {10.1002/tcr.202100022}, year = {2021}, date = {2021-09-01}, journal = {Chemical Record}, volume = {21}, number = {9}, pages = {2193-2202}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Electrochemical fluorescence switch of organic fluorescent or fluorogenic molecules Article de journal Manon Guille-Collignon; Jér^ome Delacotte; Frédéric Lema^itre; Eric Labbé; Olivier Buriez The Chemical Record, 21 (9), p. 2193–2202, 2021. @article{guille2021electrochemical, title = {Electrochemical fluorescence switch of organic fluorescent or fluorogenic molecules}, author = {Manon Guille-Collignon and J\'{e}r{^o}me Delacotte and Fr\'{e}d\'{e}ric Lema{^i}tre and Eric Labb\'{e} and Olivier Buriez}, year = {2021}, date = {2021-01-01}, journal = {The Chemical Record}, volume = {21}, number = {9}, pages = {2193--2202}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Emulsion-Templated Poly(N-Isopropylacrylamide) Shells Formed by Thermo-Enhanced Interfacial Complexation Article de journal L Sixdenier; C Tribet; E Marie ADVANCED FUNCTIONAL MATERIALS, 31 (51), p. 2105490, 2021, ISBN: 1616-301X 1616-3028 J9 - ADV FUNCT MATER. @article{Sixdenier2021b, title = {Emulsion-Templated Poly(N-Isopropylacrylamide) Shells Formed by Thermo-Enhanced Interfacial Complexation}, author = {L Sixdenier and C Tribet and E Marie}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.202105490}, doi = {10.1002/adfm.202105490}, isbn = {1616-301X 1616-3028 J9 - ADV FUNCT MATER}, year = {2021}, date = {2021-09-17}, journal = {ADVANCED FUNCTIONAL MATERIALS}, volume = {31}, number = {51}, pages = {2105490}, abstract = {The encapsulation of fragile biomacromolecules is crucial in many biotechnological applications but remains challenging. Interfacial complexation (IC) in water-in-oil emulsions proves to be an efficient process for the formation of protective polymer layers at the surface of capsule-precursor water droplets. In this work, the enhancement of conventional IC by introducing thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) strands in the interfacial polymer layer is described. Surfactant-polymer IC is implemented in water-in-fluorocarbon oil emulsions between a water-soluble poly(L-lysine)-g-poly(N-isopropylacrylamide) cationic copolymer (PLL-g-PNIPAM) and an oil-soluble anionic surfactant. Fluorescence imaging demonstrates that the thermal collapse transition of PNIPAM strands, triggered by gentle heating, induces an enrichment of the polymer layer initially formed by IC. Spontaneous co-precipitation of nanoparticles initially dispersed in the aqueous cores-with no specific treatment-is also achieved upon PNIPAM transition. This process is leveraged to irreversibly segregate these nanoparticles in the interfacial polymer layer, resulting in gel-like mixed shells. Thermo-enhancement of conventional IC is thus a promising approach for the straightforward formation, strengthening, and functionalization of capsule shells. As implemented in mild conditions, thermo-enhanced IC is additionally compatible with the encapsulation of proteins, opening new opportunities for delivery systems of biomacromolecules.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The encapsulation of fragile biomacromolecules is crucial in many biotechnological applications but remains challenging. Interfacial complexation (IC) in water-in-oil emulsions proves to be an efficient process for the formation of protective polymer layers at the surface of capsule-precursor water droplets. In this work, the enhancement of conventional IC by introducing thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) strands in the interfacial polymer layer is described. Surfactant-polymer IC is implemented in water-in-fluorocarbon oil emulsions between a water-soluble poly(L-lysine)-g-poly(N-isopropylacrylamide) cationic copolymer (PLL-g-PNIPAM) and an oil-soluble anionic surfactant. Fluorescence imaging demonstrates that the thermal collapse transition of PNIPAM strands, triggered by gentle heating, induces an enrichment of the polymer layer initially formed by IC. Spontaneous co-precipitation of nanoparticles initially dispersed in the aqueous cores-with no specific treatment-is also achieved upon PNIPAM transition. This process is leveraged to irreversibly segregate these nanoparticles in the interfacial polymer layer, resulting in gel-like mixed shells. Thermo-enhancement of conventional IC is thus a promising approach for the straightforward formation, strengthening, and functionalization of capsule shells. As implemented in mild conditions, thermo-enhanced IC is additionally compatible with the encapsulation of proteins, opening new opportunities for delivery systems of biomacromolecules. |
Finding Adapted Quinones for Harvesting Electrons from Photosynthetic Algae Suspensions Article de journal A Sayegh; L A Perego; M A Romero; L Escudero; J Delacotte; M Guille-Collignon; L Grimaud; B Bailleul; F Lemaitre Chemelectrochem, 8 (15), p. 2968-2978, 2021. @article{, title = {Finding Adapted Quinones for Harvesting Electrons from Photosynthetic Algae Suspensions}, author = {A Sayegh and L A Perego and M A Romero and L Escudero and J Delacotte and M Guille-Collignon and L Grimaud and B Bailleul and F Lemaitre}, url = {https://doi.org/10.1002/celc.202100757}, doi = {10.1002/celc.202100757}, year = {2021}, date = {2021-08-01}, journal = {Chemelectrochem}, volume = {8}, number = {15}, pages = {2968-2978}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Finding Adapted Quinones for Harvesting Electrons from Photosynthetic Algae Suspensions Article de journal A Sayegh; L A Perego; M A Romero; L Escudero; J Delacotte; M Guille-Collignon; L Grimaud; B Bailleul; F Lemaitre Chemelectrochem, 8 (15), p. 2968-2978, 2021. @article{, title = {Finding Adapted Quinones for Harvesting Electrons from Photosynthetic Algae Suspensions}, author = {A Sayegh and L A Perego and M A Romero and L Escudero and J Delacotte and M Guille-Collignon and L Grimaud and B Bailleul and F Lemaitre}, url = {https://doi.org/10.1002/celc.202100757}, doi = {10.1002/celc.202100757}, year = {2021}, date = {2021-08-01}, journal = {Chemelectrochem}, volume = {8}, number = {15}, pages = {2968-2978}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Interaction of Redox Probes and Ferrocene-labelled Peptides with Lipid Bilayers Observed at Lipid Bilayer-Modified Electrodes Article de journal Dejan Segan; George Stanley; Pierluca Messina; Jean-Marie Swiecicki; Kieu Ngo; Vincent Vivier; Olivier Buriez; Eric Labbe ChemElectroChem, 8 (13), p. 2556–2563, 2021. @article{segan2021interaction, title = {Interaction of Redox Probes and Ferrocene-labelled Peptides with Lipid Bilayers Observed at Lipid Bilayer-Modified Electrodes}, author = {Dejan Segan and George Stanley and Pierluca Messina and Jean-Marie Swiecicki and Kieu Ngo and Vincent Vivier and Olivier Buriez and Eric Labbe}, year = {2021}, date = {2021-01-01}, journal = {ChemElectroChem}, volume = {8}, number = {13}, pages = {2556--2563}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Orthogonal fluorescent chemogenetic reporters for multicolor imaging Article de journal Alison G Tebo; Benjamien Moeyaert; Marion Thauvin; Irene Carlon-Andres; Dorothea Böken; Michel Volovitch; Sergi Padilla-Parra; Peter Dedecker; Sophie Vriz; Arnaud Gautier Nature Chemical Biology, 17 (1), p. 30–38, 2021, ISSN: 1552-4469. @article{tebo_orthogonal_2021, title = {Orthogonal fluorescent chemogenetic reporters for multicolor imaging}, author = {Alison G Tebo and Benjamien Moeyaert and Marion Thauvin and Irene Carlon-Andres and Dorothea B\"{o}ken and Michel Volovitch and Sergi Padilla-Parra and Peter Dedecker and Sophie Vriz and Arnaud Gautier}, doi = {10.1038/s41589-020-0611-0}, issn = {1552-4469}, year = {2021}, date = {2021-01-01}, journal = {Nature Chemical Biology}, volume = {17}, number = {1}, pages = {30--38}, abstract = {Spectrally separated fluorophores allow the observation of multiple targets simultaneously inside living cells, leading to a deeper understanding of the molecular interplay that regulates cell function and fate. Chemogenetic systems combining a tag and a synthetic fluorophore provide certain advantages over fluorescent proteins since there is no requirement for chromophore maturation. Here, we present the engineering of a set of spectrally orthogonal fluorogen-activating tags based on the fluorescence-activating and absorption shifting tag (FAST) that are compatible with two-color, live-cell imaging. The resulting tags, greenFAST and redFAST, demonstrate orthogonality not only in their fluorogen recognition capabilities, but also in their one- and two-photon absorption profiles. This pair of orthogonal tags allowed the creation of a two-color cell cycle sensor capable of detecting very short, early cell cycles in zebrafish development and the development of split complementation systems capable of detecting multiple protein-protein interactions by live-cell fluorescence microscopy.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Spectrally separated fluorophores allow the observation of multiple targets simultaneously inside living cells, leading to a deeper understanding of the molecular interplay that regulates cell function and fate. Chemogenetic systems combining a tag and a synthetic fluorophore provide certain advantages over fluorescent proteins since there is no requirement for chromophore maturation. Here, we present the engineering of a set of spectrally orthogonal fluorogen-activating tags based on the fluorescence-activating and absorption shifting tag (FAST) that are compatible with two-color, live-cell imaging. The resulting tags, greenFAST and redFAST, demonstrate orthogonality not only in their fluorogen recognition capabilities, but also in their one- and two-photon absorption profiles. This pair of orthogonal tags allowed the creation of a two-color cell cycle sensor capable of detecting very short, early cell cycles in zebrafish development and the development of split complementation systems capable of detecting multiple protein-protein interactions by live-cell fluorescence microscopy. |
Recent Developments Concerning the Investigation of Exocytosis with Amperometry Article de journal M Guille-Collignon; F Lemaitre Current Opinion in Electrochemistry, 29 , p. 100751, 2021. @article{, title = {Recent Developments Concerning the Investigation of Exocytosis with Amperometry}, author = {M Guille-Collignon and F Lemaitre}, url = {https://doi.org/10.1016/j.coelec.2021.100751}, doi = {10.1016/j.coelec.2021.100751}, year = {2021}, date = {2021-01-01}, journal = {Current Opinion in Electrochemistry}, volume = {29}, pages = {100751}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Simulations of amperometric monitoring of exocytosis: moderate pH variations within the cell-electrode cleft with the buffer diffusion Article de journal Y Bouret; M Guille-Collignon; F Lemaitre Analytical and Bioanalytical Chemistry, 2021. @article{, title = {Simulations of amperometric monitoring of exocytosis: moderate pH variations within the cell-electrode cleft with the buffer diffusion}, author = {Y Bouret and M Guille-Collignon and F Lemaitre}, url = {https://doi.org/10.1007/s00216-021-03443-z}, doi = {10.1007/s00216-021-03443-z}, year = {2021}, date = {2021-01-01}, journal = {Analytical and Bioanalytical Chemistry}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ultrafast Dynamics of Fully Reduced Flavin in Catalytic Structures of Thymidylate Synthase ThyX Article de journal Nadia Dozova; Fabien Lacombat; Murielle Lombard; Djemel Hamdane; Pascal Plaza Physical Chemistry Chemical Physics, 23 , p. 22692-22702, 2021, ISSN: 1463-9076. @article{RN123b, title = {Ultrafast Dynamics of Fully Reduced Flavin in Catalytic Structures of Thymidylate Synthase ThyX}, author = {Nadia Dozova and Fabien Lacombat and Murielle Lombard and Djemel Hamdane and Pascal Plaza}, url = {http://dx.doi.org/10.1039/D1CP03379D}, doi = {10.1039/D1CP03379D}, issn = {1463-9076}, year = {2021}, date = {2021-01-01}, journal = {Physical Chemistry Chemical Physics}, volume = {23}, pages = {22692-22702}, keywords = {}, pubstate = {published}, tppubtype = {article} } |