You will find below the list of publications of all the members of the Peptides, Glycoconjugates and Metals in Biology research pole. For individual or theme-specific publications, please consult the research or the personal pages via the members list using the sidebar navigation tool.
2025 |
Angle-dependent spin crossover properties in polymorphic iron (ii) complexes based on pyridine--triazole derivatives Article de journal Emmelyne Cuza; Nicolas Delsuc; Jer^ome Marrot; William Shepard; Clotilde Policar; Christian Serre; Antoine Tissot Dalton Transactions, 54 (15), p. 6274–6280, 2025. @article{cuza2025angled, title = {Angle-dependent spin crossover properties in polymorphic iron (ii) complexes based on pyridine--triazole derivatives}, author = {Emmelyne Cuza and Nicolas Delsuc and Jer{^o}me Marrot and William Shepard and Clotilde Policar and Christian Serre and Antoine Tissot}, year = {2025}, date = {2025-01-01}, journal = {Dalton Transactions}, volume = {54}, number = {15}, pages = {6274--6280}, publisher = {Royal Society of Chemistry}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Angle-dependent spin crossover properties in polymorphic iron (ii) complexes based on pyridine--triazole derivatives Article de journal Emmelyne Cuza; Nicolas Delsuc; Jer^ome Marrot; William Shepard; Clotilde Policar; Christian Serre; Antoine Tissot Dalton Transactions, 54 (15), p. 6274–6280, 2025. @article{cuza2025anglec, title = {Angle-dependent spin crossover properties in polymorphic iron (ii) complexes based on pyridine--triazole derivatives}, author = {Emmelyne Cuza and Nicolas Delsuc and Jer{^o}me Marrot and William Shepard and Clotilde Policar and Christian Serre and Antoine Tissot}, year = {2025}, date = {2025-01-01}, journal = {Dalton Transactions}, volume = {54}, number = {15}, pages = {6274--6280}, publisher = {Royal Society of Chemistry}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Angle-dependent spin crossover properties in polymorphic iron (ii) complexes based on pyridine--triazole derivatives Article de journal Emmelyne Cuza; Nicolas Delsuc; Jer^ome Marrot; William Shepard; Clotilde Policar; Christian Serre; Antoine Tissot Dalton Transactions, 54 (15), p. 6274–6280, 2025. @article{cuza2025angleb, title = {Angle-dependent spin crossover properties in polymorphic iron (ii) complexes based on pyridine--triazole derivatives}, author = {Emmelyne Cuza and Nicolas Delsuc and Jer{^o}me Marrot and William Shepard and Clotilde Policar and Christian Serre and Antoine Tissot}, year = {2025}, date = {2025-01-01}, journal = {Dalton Transactions}, volume = {54}, number = {15}, pages = {6274--6280}, publisher = {Royal Society of Chemistry}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Angle-dependent spin crossover properties in polymorphic iron (ii) complexes based on pyridine--triazole derivatives Article de journal Emmelyne Cuza; Nicolas Delsuc; Jer^ome Marrot; William Shepard; Clotilde Policar; Christian Serre; Antoine Tissot Dalton Transactions, 54 (15), p. 6274–6280, 2025. @article{cuza2025angle, title = {Angle-dependent spin crossover properties in polymorphic iron (ii) complexes based on pyridine--triazole derivatives}, author = {Emmelyne Cuza and Nicolas Delsuc and Jer{^o}me Marrot and William Shepard and Clotilde Policar and Christian Serre and Antoine Tissot}, year = {2025}, date = {2025-01-01}, journal = {Dalton Transactions}, volume = {54}, number = {15}, pages = {6274--6280}, publisher = {Royal Society of Chemistry}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kinetic Redox Shotgun Proteomics Reveals Specific Lipopolysaccharide Effects on Intestinal Epithelial Cells, Mitigated by a Mn Superoxide Dismutase Mimic Article de journal Martha Zoumpoulaki; Giovanni Chiappetta; Jean Bouvet; Namita-Raju John; Gabrielle Schanne; Pauline Gehan; Samuel Diebolt; Shakir Shakir; Elodie Quévrain; Emilie Mathieu; Sylvie Demignot; Philippe Seksik; Nicolas Delsuc; Joelle Vinh; Clotilde Policar Angewandte Chemie International Edition, n/a (n/a), p. e202422644, 2025. @article{https://doi.org/10.1002/anie.202422644, title = {Kinetic Redox Shotgun Proteomics Reveals Specific Lipopolysaccharide Effects on Intestinal Epithelial Cells, Mitigated by a Mn Superoxide Dismutase Mimic}, author = {Martha Zoumpoulaki and Giovanni Chiappetta and Jean Bouvet and Namita-Raju John and Gabrielle Schanne and Pauline Gehan and Samuel Diebolt and Shakir Shakir and Elodie Qu\'{e}vrain and Emilie Mathieu and Sylvie Demignot and Philippe Seksik and Nicolas Delsuc and Joelle Vinh and Clotilde Policar}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.202422644}, doi = {https://doi.org/10.1002/anie.202422644}, year = {2025}, date = {2025-01-01}, journal = {Angewandte Chemie International Edition}, volume = {n/a}, number = {n/a}, pages = {e202422644}, abstract = {Abstract Overproduction of reactive oxygen species and antioxidant superoxide dismutases (SOD1, SOD2) dysregulation contribute to chronic inflammation such as generated in inflammatory bowel diseases (IBD). A kinetic redox shotgun proteomic strategy (OcSILAC for Oxidized cysteine Stable Isotope Labelling by Amino acids in Cell culture) was used to explore the lipopolysaccharide (LPS) effects including LPS-induced oxidation and inflammation cascades on a dedicated intestinal epithelial cell line (HT29-MD2) together with the potential mitigating role of a Mn-based SOD-mimic Mn1. While LPS induced transient oxidative damages at early times (15 min), cells incubated with Mn1 showed, in this time frame, a significantly reduced cysteine oxidation, highlighting Mn1 antioxidant properties. Over time, cysteine oxidation of LPS-treated cells was counteracted by an overexpression of antioxidant proteins (SOD1, NQO1) and a late (6 h) preponderant increase in SOD2 level. Mn1, when co-incubated with LPS, attenuated the level of most LPS-modified proteins, that is, proteins involved in the inflammatory response. Our results highlight Mn1 as a potentially effective antioxidant and anti-inflammatory agent to consider in the treatment of IBD, as well as a useful tool for exploring the interconnection between oxidative stress and inflammation.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Overproduction of reactive oxygen species and antioxidant superoxide dismutases (SOD1, SOD2) dysregulation contribute to chronic inflammation such as generated in inflammatory bowel diseases (IBD). A kinetic redox shotgun proteomic strategy (OcSILAC for Oxidized cysteine Stable Isotope Labelling by Amino acids in Cell culture) was used to explore the lipopolysaccharide (LPS) effects including LPS-induced oxidation and inflammation cascades on a dedicated intestinal epithelial cell line (HT29-MD2) together with the potential mitigating role of a Mn-based SOD-mimic Mn1. While LPS induced transient oxidative damages at early times (15 min), cells incubated with Mn1 showed, in this time frame, a significantly reduced cysteine oxidation, highlighting Mn1 antioxidant properties. Over time, cysteine oxidation of LPS-treated cells was counteracted by an overexpression of antioxidant proteins (SOD1, NQO1) and a late (6 h) preponderant increase in SOD2 level. Mn1, when co-incubated with LPS, attenuated the level of most LPS-modified proteins, that is, proteins involved in the inflammatory response. Our results highlight Mn1 as a potentially effective antioxidant and anti-inflammatory agent to consider in the treatment of IBD, as well as a useful tool for exploring the interconnection between oxidative stress and inflammation. |
SOD mimics delivered to the gut using lactic acid bacteria mitigate the colitis symptoms in a mouse model of Inflammatory Bowel Diseases Article de journal Gabrielle Schanne; Amandine Vincent; Florian Chain; Pauline Ruffié; Célia Carbonne; Elodie Quévrain; Emilie Mathieu; Alice Balfourier; Luis G Bermúdez-Humarán; Philippe Langella; Sophie Thenet; Véronique Carrière; Nassim Hammoudi; Magali Svreck; Sylvie Demignot; Philippe Seksik; Clotilde Policar; Nicolas Delsuc Free Radical Research, p. 1–16, 2025, (PMID: 40079422). @article{Schanne13032025, title = {SOD mimics delivered to the gut using lactic acid bacteria mitigate the colitis symptoms in a mouse model of Inflammatory Bowel Diseases}, author = {Gabrielle Schanne and Amandine Vincent and Florian Chain and Pauline Ruffi\'{e} and C\'{e}lia Carbonne and Elodie Qu\'{e}vrain and Emilie Mathieu and Alice Balfourier and Luis G Berm\'{u}dez-Humar\'{a}n and Philippe Langella and Sophie Thenet and V\'{e}ronique Carri\`{e}re and Nassim Hammoudi and Magali Svreck and Sylvie Demignot and Philippe Seksik and Clotilde Policar and Nicolas Delsuc}, url = {https://doi.org/10.1080/10715762.2025.2478121}, doi = {10.1080/10715762.2025.2478121}, year = {2025}, date = {2025-01-01}, journal = {Free Radical Research}, pages = {1--16}, publisher = {Taylor & Francis}, note = {PMID: 40079422}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Angle dependance Spin Crossover properties in polymorphic Iron(II) complexes based on pyridine-triazole derivatives Article de journal Emmelyne Cuza; Nicolas DELSUC; Jérome Marrot; William E Shepard; Clotilde Policar; Christian Serre; Antoine Tissot Dalton Trans., p. -, 2025. @article{D4DT03376K, title = {Angle dependance Spin Crossover properties in polymorphic Iron(II) complexes based on pyridine-triazole derivatives}, author = {Emmelyne Cuza and Nicolas DELSUC and J\'{e}rome Marrot and William E Shepard and Clotilde Policar and Christian Serre and Antoine Tissot}, url = {http://dx.doi.org/10.1039/D4DT03376K}, doi = {10.1039/D4DT03376K}, year = {2025}, date = {2025-01-01}, journal = {Dalton Trans.}, pages = {-}, publisher = {The Royal Society of Chemistry}, abstract = {Two new Fe(II) compounds based on a pyridine-triazole ligand with NCS- co-ligand were synthesized as thermodynamic (1) and kinetic (2) products. Single crystal X-Ray diffraction evidenced that those compounds are polymorphs. Magnetic susceptibility of each compound was recorded and showed that (1) undergoes a sharp spin crossover, while (2) remains high spin across the temperature range. We discussed here the importance and the impact of the crystalline packing on the switching properties of these compounds.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Two new Fe(II) compounds based on a pyridine-triazole ligand with NCS- co-ligand were synthesized as thermodynamic (1) and kinetic (2) products. Single crystal X-Ray diffraction evidenced that those compounds are polymorphs. Magnetic susceptibility of each compound was recorded and showed that (1) undergoes a sharp spin crossover, while (2) remains high spin across the temperature range. We discussed here the importance and the impact of the crystalline packing on the switching properties of these compounds. |
Locally activated semisynthetic fluorescent biosensors for imaging cellular biochemistry Article de journal Justine Coïs; Blaise Dumat Comptes Rendus. Chimie, 28 , p. 61-78, 2025, ISSN: 1878-1543. @article{Cois2024c, title = {Locally activated semisynthetic fluorescent biosensors for imaging cellular biochemistry}, author = {Justine Co\"{i}s and Blaise Dumat}, url = {https://comptes-rendus.academie-sciences.fr/chimie/articles/10.5802/crchim.365/}, doi = {10.5802/crchim.365}, issn = {1878-1543}, year = {2025}, date = {2025-01-01}, journal = {Comptes Rendus. Chimie}, volume = {28}, pages = {61-78}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Harnessing Cyanine‐like Properties to Develop Bright Fluorogenic Probes Based on Viscosity‐Sensitive Molecular Rotors Article de journal Blaise Dumat; Carolina Chieffo Chemistry – A European Journal, 31 , p. e202404077, 2025, ISSN: 0947-6539. @article{Dumat2024, title = {Harnessing Cyanine‐like Properties to Develop Bright Fluorogenic Probes Based on Viscosity‐Sensitive Molecular Rotors}, author = {Blaise Dumat and Carolina Chieffo}, url = {https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/chem.202404077}, doi = {10.1002/chem.202404077}, issn = {0947-6539}, year = {2025}, date = {2025-01-01}, journal = {Chemistry \textendash A European Journal}, volume = {31}, pages = {e202404077}, abstract = { Dipolar fluorescent molecular rotors (FMRs) are environmentally‐sensitive fluorophores that can be used in bioimaging applications to sense local viscosity and polarity. Their sensitivity to viscosity can also be used for the fluorogenic labeling of biomolecules such as DNA or proteins. In particular, we have previously used FMRs to develop a series of tunable fluorogens targeting the self‐labeling protein tag Halotag for wash‐free protein imaging in live cells. Despite these very useful properties, FMRs typically display moderate molar absorption coefficients that limits their overall fluorescence brightness. Herein, we synthesized a series of three model hemicyanines based on a styrylindolenium scaffold and performed a detailed study of their photophysical properties in solvents with various polarity and viscosity. We show that with a strong julolidine electron‐donating group it is possible to combine intense cyanine‐like absorption with the high sensitivity to viscosity of FMRs. We use this property to develop a lysosomal pH sensor and two bright cell‐impermeant fluorogens targeting HaloTag for imaging membrane proteins. We believe that this bright fluorogenic scaffold based on a simple chemical structure can be used in the future to build up a variety of probes and sensors with efficient photophysical properties. },keywords = {}, pubstate = {published}, tppubtype = {article} } <p>Dipolar fluorescent molecular rotors (FMRs) are environmentally‐sensitive fluorophores that can be used in bioimaging applications to sense local viscosity and polarity. Their sensitivity to viscosity can also be used for the fluorogenic labeling of biomolecules such as DNA or proteins. In particular, we have previously used FMRs to develop a series of tunable fluorogens targeting the self‐labeling protein tag Halotag for wash‐free protein imaging in live cells. Despite these very useful properties, FMRs typically display moderate molar absorption coefficients that limits their overall fluorescence brightness. Herein, we synthesized a series of three model hemicyanines based on a styrylindolenium scaffold and performed a detailed study of their photophysical properties in solvents with various polarity and viscosity. We show that with a strong julolidine electron‐donating group it is possible to combine intense cyanine‐like absorption with the high sensitivity to viscosity of FMRs. We use this property to develop a lysosomal pH sensor and two bright cell‐impermeant fluorogens targeting HaloTag for imaging membrane proteins. We believe that this bright fluorogenic scaffold based on a simple chemical structure can be used in the future to build up a variety of probes and sensors with efficient photophysical properties.</p> |
2024 |
A Fluorogenic Chemogenetic pH Sensor for Imaging Protein Exocytosis Article de journal Justine Coïs; Marie-Laure Niepon; Manon Wittwer; Hessam Sepasi Tehrani; Philippe Bun; Jean-Maurice Mallet; Vincent Vialou; Blaise Dumat ACS Sensors, 9 (9), p. 4690–4700, 2024, ISSN: 2379-3694. @article{Cois2024b, title = {A Fluorogenic Chemogenetic pH Sensor for Imaging Protein Exocytosis}, author = {Justine Co{\"{i}}s and Marie-Laure Niepon and Manon Wittwer and Hessam {Sepasi Tehrani} and Philippe Bun and Jean-Maurice Mallet and Vincent Vialou and Blaise Dumat}, url = {https://pubs.acs.org/doi/10.1021/acssensors.4c01057}, doi = {10.1021/acssensors.4c01057}, issn = {2379-3694}, year = {2024}, date = {2024-09-01}, journal = {ACS Sensors}, volume = {9}, number = {9}, pages = {4690--4700}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Design of Bright Chemogenetic Reporters Based on the Combined Engineering of Fluorogenic Molecular Rotors and of the HaloTag Protein Article de journal Justine Coïs; Sylvestre P J T Bachollet; Louis Sanchez; Nicolas Pietrancosta; Vincent Vialou; Jean Maurice Mallet; Blaise Dumat Chemistry - A European Journal, 30 (32), p. e202400641, 2024, ISSN: 15213765. @article{Cois2024b, title = {Design of Bright Chemogenetic Reporters Based on the Combined Engineering of Fluorogenic Molecular Rotors and of the HaloTag Protein}, author = {Justine Co{\"{i}}s and Sylvestre P J T Bachollet and Louis Sanchez and Nicolas Pietrancosta and Vincent Vialou and Jean Maurice Mallet and Blaise Dumat}, url = {https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/chem.202400641}, doi = {10.1002/chem.202400641}, issn = {15213765}, year = {2024}, date = {2024-06-01}, journal = {Chemistry - A European Journal}, volume = {30}, number = {32}, pages = {e202400641}, abstract = {The combination of fluorogenic probes (fluorogens) and self-labeling protein tags represent a promising tool for imaging biological processes with high specificity but it requires the adequation between the fluorogen and its target to ensure a good activation of its fluorescence. In this work, we report a strategy to develop molecular rotors that specifically target HaloTag with a strong enhancement of their fluorescence. The divergent design facilitates the diversification of the structures to tune the photophysical and cellular properties. Four bright fluorogens with emissions ranging from green to red were identified and applied in wash-free live cell imaging experiments with good contrast and selectivity. A HaloTag mutant adapted from previous literature reports was also tested and shown to further improve the brightness and reaction rate of the most promising fluorogen of the series both in vitro and in cells. This work opens new possibilities to develop bright chemogenetic reporters with diverse photophysical and biological properties by exploring a potentially large chemical space of simple dipolar fluorophores in combination with protein engineering.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The combination of fluorogenic probes (fluorogens) and self-labeling protein tags represent a promising tool for imaging biological processes with high specificity but it requires the adequation between the fluorogen and its target to ensure a good activation of its fluorescence. In this work, we report a strategy to develop molecular rotors that specifically target HaloTag with a strong enhancement of their fluorescence. The divergent design facilitates the diversification of the structures to tune the photophysical and cellular properties. Four bright fluorogens with emissions ranging from green to red were identified and applied in wash-free live cell imaging experiments with good contrast and selectivity. A HaloTag mutant adapted from previous literature reports was also tested and shown to further improve the brightness and reaction rate of the most promising fluorogen of the series both in vitro and in cells. This work opens new possibilities to develop bright chemogenetic reporters with diverse photophysical and biological properties by exploring a potentially large chemical space of simple dipolar fluorophores in combination with protein engineering. |
FRET-Sensing of Multivalent Protein Binding at the Interface of Biomimetic Microparticles Functionalized with Fluorescent Glycolipids Article de journal Sophie Michelis; Chiara Pompili; Florence Niedergang; Jacques Fattaccioli; Blaise Dumat; Jean-Maurice Mallet ACS Applied Materials & Interfaces, 16 (8), p. 9669–9679, 2024, ISSN: 1944-8244. @article{Michelis2024, title = {FRET-Sensing of Multivalent Protein Binding at the Interface of Biomimetic Microparticles Functionalized with Fluorescent Glycolipids}, author = {Sophie Michelis and Chiara Pompili and Florence Niedergang and Jacques Fattaccioli and Blaise Dumat and Jean-Maurice Mallet}, url = {https://pubs.acs.org/doi/10.1021/acsami.3c15067}, doi = {10.1021/acsami.3c15067}, issn = {1944-8244}, year = {2024}, date = {2024-02-01}, journal = {ACS Applied Materials & Interfaces}, volume = {16}, number = {8}, pages = {9669--9679}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Clotilde Policar; Nicolas Delsuc; Hél`ene Charlotte Bertrand Comptes Rendus. Chimie, 2024, (Online first). @article{CRCHIM_2024__27_S2_A12_0, title = {Metal complexes in cells: from design of catalytic antioxidants to imaging metal ions and designing metal-based probes in X-ray fluorescence and IR-imaging, a multidisciplinary collaborative journey in bioinorganic chemistry and inorganic chemical biology}, author = {Clotilde Policar and Nicolas Delsuc and H\'{e}l`ene Charlotte Bertrand}, doi = {10.5802/crchim.295}, year = {2024}, date = {2024-01-01}, journal = {Comptes Rendus. Chimie}, publisher = {Acad\'{e}mie des sciences, Paris}, note = {Online first}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Cellular evaluation of superoxide dismutase mimics as catalytic drugs: Challenges and opportunities Article de journal Gabrielle Schanne; Sylvie Demignot; Clotilde Policar; Nicolas Delsuc Coordination Chemistry Reviews, 514 , p. 215906, 2024, ISSN: 0010-8545. @article{SCHANNE2024215906, title = {Cellular evaluation of superoxide dismutase mimics as catalytic drugs: Challenges and opportunities}, author = {Gabrielle Schanne and Sylvie Demignot and Clotilde Policar and Nicolas Delsuc}, url = {https://www.sciencedirect.com/science/article/pii/S0010854524002522}, doi = {https://doi.org/10.1016/j.ccr.2024.215906}, issn = {0010-8545}, year = {2024}, date = {2024-01-01}, journal = {Coordination Chemistry Reviews}, volume = {514}, pages = {215906}, abstract = {Oxidative stress is known to be associated with many pathologies including inflammation, cancer, diabetes, etc. However, oxidative stress resulting from the imbalance between reactive oxygen species flows and antioxidant defenses has been largely overlooked so far as a therapeutic target. Among antioxidant defenses, superoxide dismutases (SOD) are metalloenzymes that catalyze efficiently the dismutation of superoxide, the first reactive oxygen species resulting from the monoelectronic reduction of dioxygen. Superoxide, as a quite reactive chemical species, is a transient species. So, the cellular evaluation of metal complexes mimicking SOD (SOD mimics) in cellular models can be particularly tedious and calls for multiple direct and indirect strategies including probes and biochemical assays. This review highlights methods and assays to evaluate in cells SOD mimics, a new class of catalytic antioxidants.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Oxidative stress is known to be associated with many pathologies including inflammation, cancer, diabetes, etc. However, oxidative stress resulting from the imbalance between reactive oxygen species flows and antioxidant defenses has been largely overlooked so far as a therapeutic target. Among antioxidant defenses, superoxide dismutases (SOD) are metalloenzymes that catalyze efficiently the dismutation of superoxide, the first reactive oxygen species resulting from the monoelectronic reduction of dioxygen. Superoxide, as a quite reactive chemical species, is a transient species. So, the cellular evaluation of metal complexes mimicking SOD (SOD mimics) in cellular models can be particularly tedious and calls for multiple direct and indirect strategies including probes and biochemical assays. This review highlights methods and assays to evaluate in cells SOD mimics, a new class of catalytic antioxidants. |
Bioinorganic Chemistry: A Field Where Biomimetism and Bioinspiration Are Central Article de journal Clotilde Policar Inorganic Chemistry, 63 (50), p. 23475-23478, 2024, (PMID: 39676558). @article{doi:10.1021/acs.inorgchem.4c04868, title = {Bioinorganic Chemistry: A Field Where Biomimetism and Bioinspiration Are Central}, author = {Clotilde Policar}, url = {https://doi.org/10.1021/acs.inorgchem.4c04868}, doi = {10.1021/acs.inorgchem.4c04868}, year = {2024}, date = {2024-01-01}, journal = {Inorganic Chemistry}, volume = {63}, number = {50}, pages = {23475-23478}, note = {PMID: 39676558}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2022 |
Deciphering the Metal Speciation in Low-Molecular-Weight Complexes by IMS-MS: Application to the Detection of Manganese Superoxide Dismutase Mimics in Cell Lysates Article de journal Martha Zoumpoulaki; Gabrielle Schanne; Nicolas Delsuc; Hugues Preud'homme; Elodie Quévrain; Nicolas Eskenazi; Géraldine Gazzah; Regis Guillot; Philippe Seksik; Joelle Vinh; Ryszard Lobinski; Clotilde Policar Angewandte Chemie International Edition, n/a (n/a), p. e202203066, 2022. @article{https://doi.org/10.1002/anie.202203066, title = {Deciphering the Metal Speciation in Low-Molecular-Weight Complexes by IMS-MS: Application to the Detection of Manganese Superoxide Dismutase Mimics in Cell Lysates}, author = {Martha Zoumpoulaki and Gabrielle Schanne and Nicolas Delsuc and Hugues Preud'homme and Elodie Qu\'{e}vrain and Nicolas Eskenazi and G\'{e}raldine Gazzah and Regis Guillot and Philippe Seksik and Joelle Vinh and Ryszard Lobinski and Clotilde Policar}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.202203066}, doi = {https://doi.org/10.1002/anie.202203066}, year = {2022}, date = {2022-01-01}, journal = {Angewandte Chemie International Edition}, volume = {n/a}, number = {n/a}, pages = {e202203066}, abstract = {Abstract The detection and quantification of exogenous metal complexes are crucial to understanding their activity in intricate biological media. MnII complexes are difficult to detect and quantify because of low association constants, and high lability. The superoxide dismutase (SOD) mimic (or mimetic) labelled Mn1 is based on a 1,2-di-aminoethane functionalized with imidazole and phenolate and has good intrinsic anti-superoxide, antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-activated intestinal epithelial HT29-MD2 cells, similar to that of its propylated analogue labelled Mn1P. Ion mobility spectrometry-mass spectrometry (IMS-MS) is a powerful technique for separating low molecular weight (LMW) metal complexes and can even separate complexes with the same ligand but bound to different divalent metal cations with similar ionic radii. We demonstrated the intracellular presence of the Mn1 and Mn1P complexes, at least partly intact, in lysates of cells incubated with the complexes and estimated the intracellular Mn1P concentration using a Co-13C6 analogue.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract The detection and quantification of exogenous metal complexes are crucial to understanding their activity in intricate biological media. MnII complexes are difficult to detect and quantify because of low association constants, and high lability. The superoxide dismutase (SOD) mimic (or mimetic) labelled Mn1 is based on a 1,2-di-aminoethane functionalized with imidazole and phenolate and has good intrinsic anti-superoxide, antioxidant and anti-inflammatory activities in lipopolysaccharide (LPS)-activated intestinal epithelial HT29-MD2 cells, similar to that of its propylated analogue labelled Mn1P. Ion mobility spectrometry-mass spectrometry (IMS-MS) is a powerful technique for separating low molecular weight (LMW) metal complexes and can even separate complexes with the same ligand but bound to different divalent metal cations with similar ionic radii. We demonstrated the intracellular presence of the Mn1 and Mn1P complexes, at least partly intact, in lysates of cells incubated with the complexes and estimated the intracellular Mn1P concentration using a Co-13C6 analogue. |
Cellular Detection of a Mitochondria Targeted Brominated Vinyl Triphenylamine Optical Probe (TP−Br) by X-Ray Fluorescence Microscopy Article de journal Sounderya Nagarajan; Florent Poyer; Laura Fourmois; Delphine Naud-Martin; Kadda Medjoubi; Andrea Somogyi; Gabrielle Schanne; Lucas Henry; Nicolas Delsuc; Clotilde Policar; Helene C Bertrand; Florence Mahuteau-Betzer Chemistry – A European Journal, 28 (15), p. e202104424, 2022. @article{https://doi.org/10.1002/chem.202104424, title = {Cellular Detection of a Mitochondria Targeted Brominated Vinyl Triphenylamine Optical Probe (TP−Br) by X-Ray Fluorescence Microscopy}, author = {Sounderya Nagarajan and Florent Poyer and Laura Fourmois and Delphine Naud-Martin and Kadda Medjoubi and Andrea Somogyi and Gabrielle Schanne and Lucas Henry and Nicolas Delsuc and Clotilde Policar and Helene C Bertrand and Florence Mahuteau-Betzer}, url = {https://chemistry-europe.onlinelibrary.wiley.com/doi/abs/10.1002/chem.202104424}, doi = {https://doi.org/10.1002/chem.202104424}, year = {2022}, date = {2022-01-01}, journal = {Chemistry \textendash A European Journal}, volume = {28}, number = {15}, pages = {e202104424}, abstract = {Abstract Triphenylamine (TP) derivatives such as two-branch cationic vinylbenzimidazolium triphenylamine TP−2Bzim are promising turn-on fluorescent probes suitable for two-photon imaging, labelling mitochondria in live cells. Here, we designed two TP−2Bzim derivatives as bimodal probes suitable for X-ray fluorescence imaging. The conjugation of the TP core with a rhenium tricarbonyl moiety in the TP−RePyta probe altered the localisation in live cells from mitochondria to lysosomes. The introduction of bromine on the TP core generated the TP−Br probe retaining good photophysical properties and mitochondria labelling in live cells. The influence of calcium channels in the uptake of TP−Br was studied. Synchrotron Radiation X-ray Fluorescence (SXRF) imaging of bromine enabled the detection of TP−Br and suggested a negligible presence of the probe in an unbound state in the incubated cells, a crucial point in the development of these probes. This study paves the way towards the development of TP probes as specific organelle stainers suitable for SXRF imaging.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Triphenylamine (TP) derivatives such as two-branch cationic vinylbenzimidazolium triphenylamine TP−2Bzim are promising turn-on fluorescent probes suitable for two-photon imaging, labelling mitochondria in live cells. Here, we designed two TP−2Bzim derivatives as bimodal probes suitable for X-ray fluorescence imaging. The conjugation of the TP core with a rhenium tricarbonyl moiety in the TP−RePyta probe altered the localisation in live cells from mitochondria to lysosomes. The introduction of bromine on the TP core generated the TP−Br probe retaining good photophysical properties and mitochondria labelling in live cells. The influence of calcium channels in the uptake of TP−Br was studied. Synchrotron Radiation X-ray Fluorescence (SXRF) imaging of bromine enabled the detection of TP−Br and suggested a negligible presence of the probe in an unbound state in the incubated cells, a crucial point in the development of these probes. This study paves the way towards the development of TP probes as specific organelle stainers suitable for SXRF imaging. |
SOD mimics: From the tool box of the chemists to cellular studies Article de journal Clotilde Policar; Jean Bouvet; Hélène C Bertrand; Nicolas Delsuc Current Opinion in Chemical Biology, 67 , p. 102109, 2022, ISSN: 1367-5931. @article{POLICAR2022102109, title = {SOD mimics: From the tool box of the chemists to cellular studies}, author = {Clotilde Policar and Jean Bouvet and H\'{e}l\`{e}ne C Bertrand and Nicolas Delsuc}, url = {https://www.sciencedirect.com/science/article/pii/S136759312100154X}, doi = {https://doi.org/10.1016/j.cbpa.2021.102109}, issn = {1367-5931}, year = {2022}, date = {2022-01-01}, journal = {Current Opinion in Chemical Biology}, volume = {67}, pages = {102109}, abstract = {Superoxide dismutases (SODs) are metalloproteins that protect cells against oxidative stress by controlling the concentration of superoxide (O2−) through catalysis of its dismutation. The activity of superoxide dismutases can be mimicked by low-molecular-weight complexes having potential therapeutic applications. This review presents recent strategies for designing efficient SOD mimics, from molecular metal complexes to nanomaterials. Studies of these systems in cells reveal that some SOD mimics, designed to react directly with superoxide, may also indirectly enhance the cellular antioxidant arsenal. Finally, a good understanding of the bioactivity requires information on the cell-penetration, speciation, and subcellular location of the SOD mimics: we will describe recent studies and new techniques that open opportunities for characterizing SOD mimics in biological environments.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Superoxide dismutases (SODs) are metalloproteins that protect cells against oxidative stress by controlling the concentration of superoxide (O2−) through catalysis of its dismutation. The activity of superoxide dismutases can be mimicked by low-molecular-weight complexes having potential therapeutic applications. This review presents recent strategies for designing efficient SOD mimics, from molecular metal complexes to nanomaterials. Studies of these systems in cells reveal that some SOD mimics, designed to react directly with superoxide, may also indirectly enhance the cellular antioxidant arsenal. Finally, a good understanding of the bioactivity requires information on the cell-penetration, speciation, and subcellular location of the SOD mimics: we will describe recent studies and new techniques that open opportunities for characterizing SOD mimics in biological environments. |
Inertness of Superoxide Dismutase Mimics Mn(II) Complexes Based on an Open-Chain Ligand, Bioactivity, and Detection in Intestinal Epithelial Cells Article de journal Gabrielle Schanne; Martha Zoumpoulaki; Géraldine Gazzah; Amandine Vincent; Hugues Preud’homme; Ryszard Lobinski; Sylvie Demignot; Philippe Seksik; Nicolas Delsuc; Clotilde Policar Oxidative Medicine and Cellular Longevity, 2022 , p. e3858122, 2022, ISSN: 1942-0900, (Publisher: Hindawi). @article{schanne_inertness_2022, title = {Inertness of Superoxide Dismutase Mimics Mn(II) Complexes Based on an Open-Chain Ligand, Bioactivity, and Detection in Intestinal Epithelial Cells}, author = {Gabrielle Schanne and Martha Zoumpoulaki and G\'{e}raldine Gazzah and Amandine Vincent and Hugues Preud’homme and Ryszard Lobinski and Sylvie Demignot and Philippe Seksik and Nicolas Delsuc and Clotilde Policar}, url = {https://www.hindawi.com/journals/omcl/2022/3858122/}, doi = {10.1155/2022/3858122}, issn = {1942-0900}, year = {2022}, date = {2022-01-01}, urldate = {2022-04-03}, journal = {Oxidative Medicine and Cellular Longevity}, volume = {2022}, pages = {e3858122}, abstract = {Oxidative stress is known to play a major role in the pathogenesis of inflammatory bowel diseases (IBDs), and, in particular, superoxide dismutase (SODs) defenses were shown to be weakened in patients suffering from IBDs. SOD mimics, also called SOD mimetics, as low-molecular-weight complexes reproducing the activity of SOD, constitute promising antioxidant catalytic metallodrugs in the context of IBDs. A Mn(II) complex SOD mimic (Mn1) based on an open-chain diaminoethane ligand exerting antioxidant and anti-inflammatory effects on an intestinal epithelial cellular model was shown to experience metal exchanges between the manganese center and metal ions present in the biological environment (such as Zn(II)) to some degrees. As the resulting complexes (mainly Zn(II)) were shown to be inactive, improving the kinetic inertness of Mn(II) complexes based on open-chain ligands is key to improve their bioactivity in a cellular context. We report here the study of three new Mn(II) complexes resulting from Mn1 functionalization with a cyclohexyl and/or a propyl group meant to limit, respectively, (a) metal exchanges and (b) deprotonation of an amine from the 1,2-diaminoethane central scaffold. The new manganese-based SOD mimics display a higher intrinsic SOD activity and also improved kinetic inertness in metal ion exchange processes (with Zn(II), Cu(II), Ni(II), and Co(II)). They were shown to provide anti-inflammatory and antioxidant effects in cells at lower doses than Mn1 (down to 10 μM). This improvement was due to their higher inertness against metal-assisted dissociation and not to different cellular overall accumulations. Based on its higher inertness, the SOD mimic containing both the propyl and the cyclohexyl moieties was suitable for intracellular detection and quantification by mass spectrometry, quantification, that was achieved by using a 13C-labeled Co-based analog of the SOD mimics as an external heavy standard.}, note = {Publisher: Hindawi}, keywords = {}, pubstate = {published}, tppubtype = {article} } Oxidative stress is known to play a major role in the pathogenesis of inflammatory bowel diseases (IBDs), and, in particular, superoxide dismutase (SODs) defenses were shown to be weakened in patients suffering from IBDs. SOD mimics, also called SOD mimetics, as low-molecular-weight complexes reproducing the activity of SOD, constitute promising antioxidant catalytic metallodrugs in the context of IBDs. A Mn(II) complex SOD mimic (Mn1) based on an open-chain diaminoethane ligand exerting antioxidant and anti-inflammatory effects on an intestinal epithelial cellular model was shown to experience metal exchanges between the manganese center and metal ions present in the biological environment (such as Zn(II)) to some degrees. As the resulting complexes (mainly Zn(II)) were shown to be inactive, improving the kinetic inertness of Mn(II) complexes based on open-chain ligands is key to improve their bioactivity in a cellular context. We report here the study of three new Mn(II) complexes resulting from Mn1 functionalization with a cyclohexyl and/or a propyl group meant to limit, respectively, (a) metal exchanges and (b) deprotonation of an amine from the 1,2-diaminoethane central scaffold. The new manganese-based SOD mimics display a higher intrinsic SOD activity and also improved kinetic inertness in metal ion exchange processes (with Zn(II), Cu(II), Ni(II), and Co(II)). They were shown to provide anti-inflammatory and antioxidant effects in cells at lower doses than Mn1 (down to 10 μM). This improvement was due to their higher inertness against metal-assisted dissociation and not to different cellular overall accumulations. Based on its higher inertness, the SOD mimic containing both the propyl and the cyclohexyl moieties was suitable for intracellular detection and quantification by mass spectrometry, quantification, that was achieved by using a 13C-labeled Co-based analog of the SOD mimics as an external heavy standard. |
Improvement of Peptidyl Copper Complexes Mimicking Catalase: A Subtle Balance between Thermodynamic Stability and Resistance towards H2O2 Degradation Article de journal Yaqine Ben Hadj Hammouda; Koudedja Coulibaly; Alimatou Bathily; Magdalene Teoh Sook Han; Clotilde Policar; Nicolas Delsuc Molecules, 27 (17), 2022, ISSN: 1420-3049. @article{molecules27175476, title = {Improvement of Peptidyl Copper Complexes Mimicking Catalase: A Subtle Balance between Thermodynamic Stability and Resistance towards H2O2 Degradation}, author = {Yaqine Ben Hadj Hammouda and Koudedja Coulibaly and Alimatou Bathily and Magdalene Teoh Sook Han and Clotilde Policar and Nicolas Delsuc}, url = {https://www.mdpi.com/1420-3049/27/17/5476}, doi = {10.3390/molecules27175476}, issn = {1420-3049}, year = {2022}, date = {2022-01-01}, journal = {Molecules}, volume = {27}, number = {17}, abstract = {Catalase mimics are low molecular weight metal complexes that reproduce the activity of catalase, an antioxidant metalloprotein that participates in the cellular regulation of H2O2 concentration by catalyzing its dismutation. H2O2 is a reactive oxygen species that is vital for the normal functioning of cells. However, its overproduction contributes to oxidative stress, which damages cells. Owing to their biocompatibility, peptidyl complexes are an attractive option for clinical applications to regulate H2O2 by enzyme mimics. We report here the synthesis and characterization of four new peptidyl di-copper complexes bearing two coordinating sequences. Characterization of the complexes showed that, depending on the linker used between the two coordinating sequences, their catalytic activity for H2O2 dismutation, their thermodynamic stability and their resistance to H2O2 degradation are very different, with (CATm2)Cu2 being the most promising catalyst.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Catalase mimics are low molecular weight metal complexes that reproduce the activity of catalase, an antioxidant metalloprotein that participates in the cellular regulation of H2O2 concentration by catalyzing its dismutation. H2O2 is a reactive oxygen species that is vital for the normal functioning of cells. However, its overproduction contributes to oxidative stress, which damages cells. Owing to their biocompatibility, peptidyl complexes are an attractive option for clinical applications to regulate H2O2 by enzyme mimics. We report here the synthesis and characterization of four new peptidyl di-copper complexes bearing two coordinating sequences. Characterization of the complexes showed that, depending on the linker used between the two coordinating sequences, their catalytic activity for H2O2 dismutation, their thermodynamic stability and their resistance to H2O2 degradation are very different, with (CATm2)Cu2 being the most promising catalyst. |
An expanded palette of fluorogenic HaloTag probes with enhanced contrast for targeted cellular imaging Article de journal Sylvestre P J T Bachollet; Yuriy Shpinov; Fanny Broch; Hela Benaissa; Arnaud Gautier; Nicolas Pietrancosta; Jean-Maurice Mallet; Blaise Dumat Organic & Biomolecular Chemistry, 20 (17), p. 3619 - 3628, 2022, ISSN: 1477-0520. @article{Bachollet2022, title = {An expanded palette of fluorogenic HaloTag probes with enhanced contrast for targeted cellular imaging}, author = {Sylvestre P J T Bachollet and Yuriy Shpinov and Fanny Broch and Hela Benaissa and Arnaud Gautier and Nicolas Pietrancosta and Jean-Maurice Mallet and Blaise Dumat}, url = {http://xlink.rsc.org/?DOI=D1OB02394B}, doi = {10.1039/D1OB02394B}, issn = {1477-0520}, year = {2022}, date = {2022-01-01}, journal = {Organic & Biomolecular Chemistry}, volume = {20}, number = {17}, pages = {3619 - 3628}, publisher = {Royal Society of Chemistry}, abstract = {A palette of fluorogenic molecular rotor probes with emissions from green to NIR was developed for wash-free and multicolor imaging of genetically-encoded HaloTag fusion proteins.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A palette of fluorogenic molecular rotor probes with emissions from green to NIR was developed for wash-free and multicolor imaging of genetically-encoded HaloTag fusion proteins. |
Fluorogenic and Genetic Targeting of a Red-Emitting Molecular Calcium Indicator Article de journal Sylvestre P J T Bachollet; Nicolas Pietrancosta; Jean-Maurice Mallet; Blaise Dumat Chemical Communications, 2022. @article{Bachollet2022b, title = {Fluorogenic and Genetic Targeting of a Red-Emitting Molecular Calcium Indicator}, author = {Sylvestre P J T Bachollet and Nicolas Pietrancosta and Jean-Maurice Mallet and Blaise Dumat}, doi = {10.1039/D2CC01792J}, year = {2022}, date = {2022-01-01}, journal = {Chemical Communications}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2021 |
Finding Adapted Quinones for Harvesting Electrons from Photosynthetic Algae Suspensions Article de journal A Sayegh; L A Perego; M A Romero; L Escudero; J Delacotte; M Guille-Collignon; L Grimaud; B Bailleul; F Lemaitre Chemelectrochem, 8 (15), p. 2968-2978, 2021. @article{, title = {Finding Adapted Quinones for Harvesting Electrons from Photosynthetic Algae Suspensions}, author = {A Sayegh and L A Perego and M A Romero and L Escudero and J Delacotte and M Guille-Collignon and L Grimaud and B Bailleul and F Lemaitre}, url = {https://doi.org/10.1002/celc.202100757}, doi = {10.1002/celc.202100757}, year = {2021}, date = {2021-08-01}, journal = {Chemelectrochem}, volume = {8}, number = {15}, pages = {2968-2978}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Finding Adapted Quinones for Harvesting Electrons from Photosynthetic Algae Suspensions Article de journal A Sayegh; L A Perego; M A Romero; L Escudero; J Delacotte; M Guille-Collignon; L Grimaud; B Bailleul; F Lemaitre Chemelectrochem, 8 (15), p. 2968-2978, 2021. @article{, title = {Finding Adapted Quinones for Harvesting Electrons from Photosynthetic Algae Suspensions}, author = {A Sayegh and L A Perego and M A Romero and L Escudero and J Delacotte and M Guille-Collignon and L Grimaud and B Bailleul and F Lemaitre}, url = {https://doi.org/10.1002/celc.202100757}, doi = {10.1002/celc.202100757}, year = {2021}, date = {2021-08-01}, journal = {Chemelectrochem}, volume = {8}, number = {15}, pages = {2968-2978}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
A di-Copper Peptidyl Complex Mimics the Activity of Catalase, a Key Antioxidant Metalloenzyme Article de journal Koudedja Coulibaly; Marion Thauvin; Adyn Melenbacher; Clara Testard; Evangelina Trigoni; Amandine Vincent; Martin J Stillman; Sophie Vriz; Clotilde Policar; Nicolas Delsuc Inorganic Chemistry, 60 (13), p. 9309-9319, 2021. @article{doi:10.1021/acs.inorgchem.0c03718b, title = {A di-Copper Peptidyl Complex Mimics the Activity of Catalase, a Key Antioxidant Metalloenzyme}, author = {Koudedja Coulibaly and Marion Thauvin and Adyn Melenbacher and Clara Testard and Evangelina Trigoni and Amandine Vincent and Martin J Stillman and Sophie Vriz and Clotilde Policar and Nicolas Delsuc}, url = {https://doi.org/10.1021/acs.inorgchem.0c03718}, doi = {10.1021/acs.inorgchem.0c03718}, year = {2021}, date = {2021-01-01}, journal = {Inorganic Chemistry}, volume = {60}, number = {13}, pages = {9309-9319}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Rhenium carbonyl complexes bearing methylated triphenylphosphonium cations as antibody-free mitochondria trackers for X-ray fluorescence imaging Article de journal Gabrielle Schanne; Lucas Henry; How Chee Ong; Andrea Somogyi; Kadda Medjoubi; Nicolas Delsuc; Clotilde Policar; Felipe García; Helene C Bertrand Inorg. Chem. Front., 8 , p. 3905-3915, 2021. @article{D1QI00542A, title = {Rhenium carbonyl complexes bearing methylated triphenylphosphonium cations as antibody-free mitochondria trackers for X-ray fluorescence imaging}, author = {Gabrielle Schanne and Lucas Henry and How Chee Ong and Andrea Somogyi and Kadda Medjoubi and Nicolas Delsuc and Clotilde Policar and Felipe Garc\'{i}a and Helene C Bertrand}, url = {http://dx.doi.org/10.1039/D1QI00542A}, doi = {10.1039/D1QI00542A}, year = {2021}, date = {2021-01-01}, journal = {Inorg. Chem. Front.}, volume = {8}, pages = {3905-3915}, publisher = {The Royal Society of Chemistry}, abstract = {Synchrotron Radiation X-ray Fluorescence (SXRF) imaging is a powerful technique for the visualization of metal complexes in biological systems. However, due to the lack of an endogenous elemental signature for mitochondria, probes for the localization of this organelle are required for colocalization studies. In this work, we designed and synthesized rhenium pyta tricarbonyl complexes conjugated to methylated triphenylphosphonium TP*P+ cations as multimodal probes for the visualization of mitochondria, suitable for fluorescence and SXRF imaging and quantification. Accumulation of the methylated triphenylphosphonium TP*P+-based conjugates in cells was observed in fixed A549 cells, and the amount of mitochondrial uptake was linked to the lipophilicity of the TPP+ vector. Our work highlights a convenient rhenium-based multimodal mitochondrial-targeted probe compatible with SXRF nano-imaging.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Synchrotron Radiation X-ray Fluorescence (SXRF) imaging is a powerful technique for the visualization of metal complexes in biological systems. However, due to the lack of an endogenous elemental signature for mitochondria, probes for the localization of this organelle are required for colocalization studies. In this work, we designed and synthesized rhenium pyta tricarbonyl complexes conjugated to methylated triphenylphosphonium TP*P+ cations as multimodal probes for the visualization of mitochondria, suitable for fluorescence and SXRF imaging and quantification. Accumulation of the methylated triphenylphosphonium TP*P+-based conjugates in cells was observed in fixed A549 cells, and the amount of mitochondrial uptake was linked to the lipophilicity of the TPP+ vector. Our work highlights a convenient rhenium-based multimodal mitochondrial-targeted probe compatible with SXRF nano-imaging. |
Evaluation of the compounds commonly known as superoxide dismutase and catalase mimics in cellular models Article de journal Amandine Vincent; Marion Thauvin; Elodie Quévrain; Emilie Mathieu; Sarah Layani; Philippe Seksik; Ines Batinic-Haberle; Sophie Vriz; Clotilde Policar; Nicolas Delsuc Journal of Inorganic Biochemistry, p. 111431, 2021, ISSN: 0162-0134. @article{VINCENT2021111431, title = {Evaluation of the compounds commonly known as superoxide dismutase and catalase mimics in cellular models}, author = {Amandine Vincent and Marion Thauvin and Elodie Qu\'{e}vrain and Emilie Mathieu and Sarah Layani and Philippe Seksik and Ines Batinic-Haberle and Sophie Vriz and Clotilde Policar and Nicolas Delsuc}, url = {https://www.sciencedirect.com/science/article/pii/S0162013421000787}, doi = {https://doi.org/10.1016/j.jinorgbio.2021.111431}, issn = {0162-0134}, year = {2021}, date = {2021-01-01}, journal = {Journal of Inorganic Biochemistry}, pages = {111431}, abstract = {Oxidative stress that results from an imbalance between the concentrations of reactive species (RS) and antioxidant defenses is associated with many pathologies. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase are among the key enzymes that maintain the low nanomolar physiological concentrations of superoxide and hydrogen peroxide. The increase in the levels of these species and their progeny could have deleterious effects. In this context, chemists have developed SOD and CAT mimics to supplement them when cells are overwhelmed with oxidative stress. However, the beneficial activity of such molecules in cells depends not only on their intrinsic catalytic activities but also on their stability in biological context, their cell penetration and their cellular localization. We have employed cellular assays to characterize several compounds that possess SOD and CAT activities and have been frequently used in cellular and animal models. We used cellular assays that address SOD and CAT activities of the compounds. Finally, we determined the effect of compounds on the suppression of the inflammation in HT29-MD2 cells challenged by lipopolysaccharide. When the assay requires penetration inside cells, the SOD mimics Mn(III) meso-tetrakis(N-(2′-nbutoxyethyl)pyridinium-2-yl)porphyrin (MnTnBuOE-2-PyP5+) and Mn(II) dichloro[(4aR,13aR,17aR,21aR)-1,2,3,4,4a,5, 6,12,13,13a,14,15,16,17,17a,18,19,20,21,21a-eicosahydro-11,7-nitrilo-7Hdibenzo[b,h] [1, 4, 7, 10] tetra--azacycloheptadecine-κN5,κN13,κN18,κN21,κN22] (Imisopasem manganese, M40403, CG4419) were found efficacious at 10 μM, while Mn(II) chloro N-(phenolato)-N,N′-bis[2-(N-methyl-imidazolyl)methyl]-ethane-1,2-diamine (Mn1) requires an incubation at 100 μM. This study thus demonstrates that MnTnBuOE-2-PyP5+, M40403 and Mn1 were efficacious in suppressing inflammatory response in HT29-MD2 cells and such action appears to be related to their ability to enter the cells and modulate reactive oxygen species (ROS) levels.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Oxidative stress that results from an imbalance between the concentrations of reactive species (RS) and antioxidant defenses is associated with many pathologies. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase are among the key enzymes that maintain the low nanomolar physiological concentrations of superoxide and hydrogen peroxide. The increase in the levels of these species and their progeny could have deleterious effects. In this context, chemists have developed SOD and CAT mimics to supplement them when cells are overwhelmed with oxidative stress. However, the beneficial activity of such molecules in cells depends not only on their intrinsic catalytic activities but also on their stability in biological context, their cell penetration and their cellular localization. We have employed cellular assays to characterize several compounds that possess SOD and CAT activities and have been frequently used in cellular and animal models. We used cellular assays that address SOD and CAT activities of the compounds. Finally, we determined the effect of compounds on the suppression of the inflammation in HT29-MD2 cells challenged by lipopolysaccharide. When the assay requires penetration inside cells, the SOD mimics Mn(III) meso-tetrakis(N-(2′-nbutoxyethyl)pyridinium-2-yl)porphyrin (MnTnBuOE-2-PyP5+) and Mn(II) dichloro[(4aR,13aR,17aR,21aR)-1,2,3,4,4a,5, 6,12,13,13a,14,15,16,17,17a,18,19,20,21,21a-eicosahydro-11,7-nitrilo-7Hdibenzo[b,h] [1, 4, 7, 10] tetra--azacycloheptadecine-κN5,κN13,κN18,κN21,κN22] (Imisopasem manganese, M40403, CG4419) were found efficacious at 10 μM, while Mn(II) chloro N-(phenolato)-N,N′-bis[2-(N-methyl-imidazolyl)methyl]-ethane-1,2-diamine (Mn1) requires an incubation at 100 μM. This study thus demonstrates that MnTnBuOE-2-PyP5+, M40403 and Mn1 were efficacious in suppressing inflammatory response in HT29-MD2 cells and such action appears to be related to their ability to enter the cells and modulate reactive oxygen species (ROS) levels. |
2020 |
Fluorogenic Protein Probes with Red and Near‐Infrared Emission for Genetically Targeted Imaging** Article de journal Sylvestre P J T Bachollet; Cyril Addi; Nicolas Pietrancosta; Jean-Maurice Mallet; Blaise Dumat Chemistry – A European Journal, 26 (63), p. 14467–14473, 2020, ISSN: 0947-6539. @article{Bachollet2020, title = {Fluorogenic Protein Probes with Red and Near‐Infrared Emission for Genetically Targeted Imaging**}, author = {Sylvestre P J T Bachollet and Cyril Addi and Nicolas Pietrancosta and Jean-Maurice Mallet and Blaise Dumat}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/chem.202002911 https://onlinelibrary.wiley.com/doi/10.1002/chem.202002911}, doi = {10.1002/chem.202002911}, issn = {0947-6539}, year = {2020}, date = {2020-11-01}, journal = {Chemistry \textendash A European Journal}, volume = {26}, number = {63}, pages = {14467--14473}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
An easy-to-implement combinatorial approach involving an activity-based assay for the discovery of a peptidyl copper complex mimicking superoxide dismutase Article de journal Amandine Vincent; Jennifer Rodon Fores; Elodie Tauziet; Elodie Quévrain; Ágnes Dancs; Amandine Conte-Daban; Anne-Sophie Bernard; Philippe Pelupessy; Koudedja Coulibaly; Philippe Seksik; Christelle Hureau; Katalin Selmeczi; Clotilde Policar; Nicolas Delsuc Chem. Commun., 56 , p. 399-402, 2020. @article{C9CC07920C, title = {An easy-to-implement combinatorial approach involving an activity-based assay for the discovery of a peptidyl copper complex mimicking superoxide dismutase}, author = {Amandine Vincent and Jennifer Rodon Fores and Elodie Tauziet and Elodie Qu\'{e}vrain and \'{A}gnes Dancs and Amandine Conte-Daban and Anne-Sophie Bernard and Philippe Pelupessy and Koudedja Coulibaly and Philippe Seksik and Christelle Hureau and Katalin Selmeczi and Clotilde Policar and Nicolas Delsuc}, url = {http://dx.doi.org/10.1039/C9CC07920C}, doi = {10.1039/C9CC07920C}, year = {2020}, date = {2020-01-01}, journal = {Chem. Commun.}, volume = {56}, pages = {399-402}, publisher = {The Royal Society of Chemistry}, abstract = {A combinatorial approach using a one-bead-one-compound method and a screening based on a SOD-activity assay was set up for the discovery of an efficient peptidyl copper complex. The complex exhibited good stability constants, suitable redox potentials and excellent intrinsic activity. This complex was further assayed in cells for its antioxidant properties and showed beneficial effects when cells were subjected to oxidative stress.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A combinatorial approach using a one-bead-one-compound method and a screening based on a SOD-activity assay was set up for the discovery of an efficient peptidyl copper complex. The complex exhibited good stability constants, suitable redox potentials and excellent intrinsic activity. This complex was further assayed in cells for its antioxidant properties and showed beneficial effects when cells were subjected to oxidative stress. |
Intracellular location matters: rationalization of the anti-inflammatory activity of a manganese(ii) superoxide dismutase mimic complex Article de journal Emilie Mathieu; Anne-Sophie Bernard; Elodie Quévrain; Martha Zoumpoulaki; Sébastien Iriart; Caroline Lung-Soong; Barry Lai; Kadda Medjoubi; Lucas Henry; Sounderya Nagarajan; Florent Poyer; Andreas Scheitler; Ivana Ivanović-Burmazović; Sergio Marco; Andrea Somogyi; Philippe Seksik; Nicolas Delsuc; Clotilde Policar Chem. Commun., p. -, 2020. @article{D0CC03398G, title = {Intracellular location matters: rationalization of the anti-inflammatory activity of a manganese(ii) superoxide dismutase mimic complex}, author = {Emilie Mathieu and Anne-Sophie Bernard and Elodie Qu\'{e}vrain and Martha Zoumpoulaki and S\'{e}bastien Iriart and Caroline Lung-Soong and Barry Lai and Kadda Medjoubi and Lucas Henry and Sounderya Nagarajan and Florent Poyer and Andreas Scheitler and Ivana Ivanovi\'{c}-Burmazovi\'{c} and Sergio Marco and Andrea Somogyi and Philippe Seksik and Nicolas Delsuc and Clotilde Policar}, url = {http://dx.doi.org/10.1039/D0CC03398G}, doi = {10.1039/D0CC03398G}, year = {2020}, date = {2020-01-01}, journal = {Chem. Commun.}, pages = {-}, publisher = {The Royal Society of Chemistry}, abstract = {A conjugate of a Mn-based superoxide dismutase mimic with a Re-based multimodal probe ̲ was studied in a cellular model of oxidative stress. Its speciation was investigated using Re and Mn X-fluorescence. Interestingly, ̲ shows a distribution different from its unconjugated analogue but a similar concentration in mitochondria and a similar bioactivity.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A conjugate of a Mn-based superoxide dismutase mimic with a Re-based multimodal probe ̲ was studied in a cellular model of oxidative stress. Its speciation was investigated using Re and Mn X-fluorescence. Interestingly, ̲ shows a distribution different from its unconjugated analogue but a similar concentration in mitochondria and a similar bioactivity. |