Microfluidic device with integrated microfilter of conical-shaped holes for high efficiency and high purity capture of circulating tumor cells

Printer-friendly version
TitleMicrofluidic device with integrated microfilter of conical-shaped holes for high efficiency and high purity capture of circulating tumor cells
Publication TypeJournal Article
Year of Publication2014
AuthorsTang Y, Jian Shi, Sisi Li, Li Wang, Cayre YE, Chen Y
JournalScientific Reports
Volume4
Date Published8/2014
Accession Number6052
AbstractCapture of circulating tumor cells (CTCs) from peripheral blood of cancer patients has major implications for metastatic detection and therapy analyses. Here we demonstrated a microfluidic device for high efficiency and high purity capture of CTCs. The key novelty of this approach lies on the integration of a microfilter with conical-shaped holes and a micro-injector with cross-flow components for size dependent capture of tumor cells without significant retention of non-tumor cells. Under conditions of constant flow rate, tumor cells spiked into phosphate buffered saline could be recovered and then cultured for further analyses. When tumor cells were spiked in blood of healthy donors, they could also be recovered at high efficiency and high clearance efficiency of white blood cells. When the same device was used for clinical validation, CTCs could be detected in blood samples of cancer patients but not in that of healthy donors. Finally, the capture efficiency of tumor cells is cell-type dependent but the hole size of the filter should be more closely correlated to the nuclei size of the tumor cells. Together with the advantage of easy operation, low-cost and high potential of integration, this approach offers unprecedented opportunities for metastatic detection and cancer treatment monitoring.
URLhttp://www.nature.com/srep/2014/140813/srep06052/full/srep06052.html
DOI10.1038/srep06052
Short TitleSci. Rep.
Unit: 
UMR 8640