Sequence-independent and reversible photocontrol of transcription/expression systems using a photosensitive nucleic acid binder

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TitleSequence-independent and reversible photocontrol of transcription/expression systems using a photosensitive nucleic acid binder
Publication TypeJournal Article
Year of Publication2009
AuthorsEstevez-Torres, A, Crozatier C, Diguet A, Hara T, Saito H, Yoshikawa K, Baigl D
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue30
Pagination12219-12223
Date PublishedJul
ISBN Number0027-8424
Accession NumberISI:000268440200005
Abstract

To understand non-trivial biological functions, it is crucial to develop minimal synthetic models that capture their basic features. Here, we demonstrate a sequence-independent, reversible control of transcription and gene expression using a photosensitive nucleic acid binder (pNAB). By introducing a pNAB whose affinity for nucleic acids is tuned by light, in vitro RNA production, EGFP translation, and GFP expression (a set of reactions including both transcription and translation) were successfully inhibited in the dark and recovered after a short illumination at 365 nm. Our results indicate that the accessibility of the protein machinery to one or several nucleic acid binding sites can be efficiently regulated by changing the conformational/condensation state of the nucleic acid (DNA conformation or mRNA aggregation), thus regulating gene activity in an efficient, reversible, and sequence-independent manner. The possibility offered by our approach to use light to trigger various gene expression systems in a system-independent way opens interesting perspectives to study gene expression dynamics as well as to develop photocontrolled biotechnological procedures.

URL<Go to ISI>://000268440200005
DOI10.1073/pnas.0904382106
Short TitleSequence-independent and reversible photocontrol of transcription/expression systems using a photosensitive nucleic acid binder
Unit: 
UMR 8640