MALDI-TOF mass spectrometry: A powerful tool to study the internalization of cell-penetrating peptides

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TitleMALDI-TOF mass spectrometry: A powerful tool to study the internalization of cell-penetrating peptides
Publication TypeJournal Article
Year of Publication2010
AuthorsAubry S, Aussedat B, Delaroche D, Jiao CY, Bolbach G, Lavielle S, Chassaing G, Sagan S, Burlina F
JournalBiochimica Et Biophysica Acta-Biomembranes
Volume1798
Issue12
Pagination2182-2189
Date PublishedDec
ISBN Number0005-2736
Accession NumberISI:000284392900002
Abstract

This review summarizes the contribution of MALDI-TOF mass spectrometry in the study of cell-penetrating peptide (CPP) internalization in eukaryote cells. This technique was used to measure the efficiency of cell-penetrating peptide cellular uptake and cargo delivery and to analyze carrier and cargo intracellular degradation. The impact of thiol-containing membrane proteins on the internalization of CPP-cargo disulfide conjugates was also evaluated by combining MALDI-TOF MS with simple thiol-specific reactions. This highlighted the formation of cross-linked species to cell-surface proteins that either remained trapped in the cell membrane or led to intracellular delivery. MALDI-TOF MS is thus a powerful tool to dissect CPP internalization mechanisms. (C) 2009 Elsevier B.V. All rights reserved.

URL<Go to ISI>://000284392900002
DOI10.1016/j.bbamem.2009.11.011
Short TitleMALDI-TOF mass spectrometry: A powerful tool to study the internalization of cell-penetrating peptides
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