You will find below the publication list of our pole.
For the publication list of each pole member, please see his/her personal webpage.
2018 |
Fast and complete electrochemical conversion of solutes contained in micro-volume water droplets Article de journal L Godeffroy; F Chau; O Buriez; E Labbé Electrochemistry Communications, 86 , p. 145–148, 2018. @article{Godeffroy:2018, title = {Fast and complete electrochemical conversion of solutes contained in micro-volume water droplets}, author = {L Godeffroy and F Chau and O Buriez and E Labb\'{e}}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85038019547&doi=10.1016%2fj.elecom.2017.12.007&partnerID=40&md5=e4be97ff9584068f86e05922768a8567}, doi = {10.1016/j.elecom.2017.12.007}, year = {2018}, date = {2018-01-01}, journal = {Electrochemistry Communications}, volume = {86}, pages = {145--148}, abstract = {An elegant hanging-droplet or meniscus-based setup is proposed to carry out quantitative electrolyses from either an organic (hydroquinone) or an inorganic (permanganate) substrate. These examples validate the concept of using such easily accessible, fast (1\textendash3 min) and low-cost operating conditions not only for preparative applications (electrosynthesis), but also for pedagogical purposes in minute samples. © 2017 Elsevier B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } An elegant hanging-droplet or meniscus-based setup is proposed to carry out quantitative electrolyses from either an organic (hydroquinone) or an inorganic (permanganate) substrate. These examples validate the concept of using such easily accessible, fast (1–3 min) and low-cost operating conditions not only for preparative applications (electrosynthesis), but also for pedagogical purposes in minute samples. © 2017 Elsevier B.V. |
Fluorogenic Probing of Membrane Protein Trafficking Article de journal C Li; A Mourton; M -A Plamont; V Rodrigues; I Aujard; M Volovitch; T Le Saux; F Perez; S Vriz; L Jullien; A Joliot; A Gautier Bioconjugate Chemistry, 29 (6), p. 1823–1828, 2018. @article{Li:2018a, title = {Fluorogenic Probing of Membrane Protein Trafficking}, author = {C Li and A Mourton and M -A Plamont and V Rodrigues and I Aujard and M Volovitch and T Le Saux and F Perez and S Vriz and L Jullien and A Joliot and A Gautier}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85047641063&doi=10.1021%2facs.bioconjchem.8b00180&partnerID=40&md5=bcbb74499b742fa8b0762b8c52ebf5dd}, doi = {10.1021/acs.bioconjchem.8b00180}, year = {2018}, date = {2018-01-01}, journal = {Bioconjugate Chemistry}, volume = {29}, number = {6}, pages = {1823--1828}, abstract = {Methods to differentially label cell-surface and intracellular membrane proteins are indispensable for understanding their function and the regulation of their trafficking. We present an efficient strategy for the rapid and selective fluorescent labeling of membrane proteins based on the chemical-genetic fluorescent marker FAST (fluorescence-activating and absorption-shifting tag). Cell-surface FAST-tagged proteins could be selectively and rapidly labeled using fluorogenic membrane-impermeant 4-hydroxybenzylidene rhodanine (HBR) analogs. This approach allows the study of protein trafficking at the plasma membrane with various fluorometric techniques, and opens exciting prospects for the high-throughput screening of small molecules able to restore disease-related trafficking defects. © 2018 American Chemical Society.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Methods to differentially label cell-surface and intracellular membrane proteins are indispensable for understanding their function and the regulation of their trafficking. We present an efficient strategy for the rapid and selective fluorescent labeling of membrane proteins based on the chemical-genetic fluorescent marker FAST (fluorescence-activating and absorption-shifting tag). Cell-surface FAST-tagged proteins could be selectively and rapidly labeled using fluorogenic membrane-impermeant 4-hydroxybenzylidene rhodanine (HBR) analogs. This approach allows the study of protein trafficking at the plasma membrane with various fluorometric techniques, and opens exciting prospects for the high-throughput screening of small molecules able to restore disease-related trafficking defects. © 2018 American Chemical Society. |
Fluorogenic Protein-Based Strategies for Detection, Actuation, and Sensing Article de journal A Gautier; A G Tebo BioEssays, 40 (10), 2018. @article{Gautier:2018, title = {Fluorogenic Protein-Based Strategies for Detection, Actuation, and Sensing}, author = {A Gautier and A G Tebo}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053180336&doi=10.1002%2fbies.201800118&partnerID=40&md5=92409e91810fa2bf17d3eef357bc1bc9}, doi = {10.1002/bies.201800118}, year = {2018}, date = {2018-01-01}, journal = {BioEssays}, volume = {40}, number = {10}, abstract = {Fluorescence imaging has become an indispensable tool in cell and molecular biology. GFP-like fluorescent proteins have revolutionized fluorescence microscopy, giving experimenters exquisite control over the localization and specificity of tagged constructs. However, these systems present certain drawbacks and as such, alternative systems based on a fluorogenic interaction between a chromophore and a protein have been developed. While these systems are initially designed as fluorescent labels, they also present new opportunities for the development of novel labeling and detection strategies. This review focuses on new labeling protocols, actuation methods, and biosensors based on fluorogenic protein systems. © 2018 WILEY Periodicals, Inc.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Fluorescence imaging has become an indispensable tool in cell and molecular biology. GFP-like fluorescent proteins have revolutionized fluorescence microscopy, giving experimenters exquisite control over the localization and specificity of tagged constructs. However, these systems present certain drawbacks and as such, alternative systems based on a fluorogenic interaction between a chromophore and a protein have been developed. While these systems are initially designed as fluorescent labels, they also present new opportunities for the development of novel labeling and detection strategies. This review focuses on new labeling protocols, actuation methods, and biosensors based on fluorogenic protein systems. © 2018 WILEY Periodicals, Inc. |
Improved Chemical-Genetic Fluorescent Markers for Live Cell Microscopy Article de journal A G Tebo; F M Pimenta; Y Zhang; A Gautier Biochemistry, 57 (39), p. 5648–5653, 2018. @article{Tebo:2018a, title = {Improved Chemical-Genetic Fluorescent Markers for Live Cell Microscopy}, author = {A G Tebo and F M Pimenta and Y Zhang and A Gautier}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053681817&doi=10.1021%2facs.biochem.8b00649&partnerID=40&md5=550c3f9cf88557339074b67771afd7d7}, doi = {10.1021/acs.biochem.8b00649}, year = {2018}, date = {2018-01-01}, journal = {Biochemistry}, volume = {57}, number = {39}, pages = {5648--5653}, abstract = {Inducible chemical-genetic fluorescent markers are promising tools for live cell imaging requiring high spatiotemporal resolution and low background fluorescence. The fluorescence-activating and absorption shifting tag (FAST) was recently developed to form fluorescent molecular complexes with a family of small, synthetic fluorogenic chromophores (so-called fluorogens). Here, we use rational design to modify the binding pocket of the protein and screen for improved fluorescence performances with four different fluorogens. The introduction of a single mutation results in improvements in both quantum yield and dissociation constant with nearly all fluorogens tested. Our improved FAST (iFAST) allowed the generation of a tandem iFAST (td-iFAST) that forms green and red fluorescent reporters 1.6-fold and 2-fold brighter than EGFP and mCherry, respectively, while having a comparable size. © Copyright 2018 American Chemical Society.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Inducible chemical-genetic fluorescent markers are promising tools for live cell imaging requiring high spatiotemporal resolution and low background fluorescence. The fluorescence-activating and absorption shifting tag (FAST) was recently developed to form fluorescent molecular complexes with a family of small, synthetic fluorogenic chromophores (so-called fluorogens). Here, we use rational design to modify the binding pocket of the protein and screen for improved fluorescence performances with four different fluorogens. The introduction of a single mutation results in improvements in both quantum yield and dissociation constant with nearly all fluorogens tested. Our improved FAST (iFAST) allowed the generation of a tandem iFAST (td-iFAST) that forms green and red fluorescent reporters 1.6-fold and 2-fold brighter than EGFP and mCherry, respectively, while having a comparable size. © Copyright 2018 American Chemical Society. |
Investigation of photocurrents resulting from a living unicellular algae suspension with quinones over time Article de journal G Longatte; A Sayegh; J Delacotte; F Rappaport; F -A Wollman; M Guille-Collignon; F Lemaître Chemical Science, 9 (43), p. 8271–8281, 2018. @article{Longatte:2018, title = {Investigation of photocurrents resulting from a living unicellular algae suspension with quinones over time}, author = {G Longatte and A Sayegh and J Delacotte and F Rappaport and F -A Wollman and M Guille-Collignon and F Lema\^{i}tre}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85056308267&doi=10.1039%2fc8sc03058h&partnerID=40&md5=73d658b7ab313cc1a772ca28dc56aa2d}, doi = {10.1039/c8sc03058h}, year = {2018}, date = {2018-01-01}, journal = {Chemical Science}, volume = {9}, number = {43}, pages = {8271--8281}, abstract = {Plants, algae, and some bacteria convert solar energy into chemical energy by using photosynthesis. In light of the current energy environment, many research strategies try to benefit from photosynthesis in order to generate usable photobioelectricity. Among all the strategies developed for transferring electrons from the photosynthetic chain to an outer collecting electrode, we recently implemented a method on a preparative scale (high surface electrode) based on a Chlamydomonas reinhardtii green algae suspension in the presence of exogenous quinones as redox mediators. While giving rise to an interesting performance (10-60 μA cm-2) in the course of one hour, this device appears to cause a slow decrease of the recorded photocurrent. In this paper, we wish to analyze and understand this gradual fall in performance in order to limit this issue in future applications. We thus first show that this kind of degradation could be related to over-irradiation conditions or side-effects of quinones depending on experimental conditions. We therefore built an empirical model involving a kinetic quenching induced by incubation with quinones, which is globally consistent with the experimental data provided by fluorescence measurements achieved after dark incubation of algae in the presence of quinones. © 2018 The Royal Society of Chemistry.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Plants, algae, and some bacteria convert solar energy into chemical energy by using photosynthesis. In light of the current energy environment, many research strategies try to benefit from photosynthesis in order to generate usable photobioelectricity. Among all the strategies developed for transferring electrons from the photosynthetic chain to an outer collecting electrode, we recently implemented a method on a preparative scale (high surface electrode) based on a Chlamydomonas reinhardtii green algae suspension in the presence of exogenous quinones as redox mediators. While giving rise to an interesting performance (10-60 μA cm-2) in the course of one hour, this device appears to cause a slow decrease of the recorded photocurrent. In this paper, we wish to analyze and understand this gradual fall in performance in order to limit this issue in future applications. We thus first show that this kind of degradation could be related to over-irradiation conditions or side-effects of quinones depending on experimental conditions. We therefore built an empirical model involving a kinetic quenching induced by incubation with quinones, which is globally consistent with the experimental data provided by fluorescence measurements achieved after dark incubation of algae in the presence of quinones. © 2018 The Royal Society of Chemistry. |
Light-assisted dynamic titration: from theory to an experimental protocol Article de journal A Pellissier-Tanon; R Chouket; T Le Saux; L Jullien; A Lemarchand Physical Chemistry Chemical Physics, 20 (37), p. 23998–24010, 2018. @article{Pellissier-Tanon:2018, title = {Light-assisted dynamic titration: from theory to an experimental protocol}, author = {A Pellissier-Tanon and R Chouket and T Le Saux and L Jullien and A Lemarchand}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85054097721&doi=10.1039%2fc8cp03953d&partnerID=40&md5=782f885754810c59f73c4a3134f6355f}, doi = {10.1039/c8cp03953d}, year = {2018}, date = {2018-01-01}, journal = {Physical Chemistry Chemical Physics}, volume = {20}, number = {37}, pages = {23998--24010}, abstract = {In order to design a dynamic titration method, we propose a theoretical model harnessing the kinetic properties of the complexation of the titrated species with a titrating photoswitchable reagent. Forced oscillations of illumination are imposed and concentration oscillations of the targeted species are deduced from the equations of chemical kinetics. We determine analytical expressions of the resonance conditions on the control parameters, angular frequency, mean light intensity, and total concentration of the photoswitchable reagent, which optimize the out-of-phase amplitude of concentration oscillations. A user-friendly protocol of dynamic titration is proposed. © the Owner Societies.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In order to design a dynamic titration method, we propose a theoretical model harnessing the kinetic properties of the complexation of the titrated species with a titrating photoswitchable reagent. Forced oscillations of illumination are imposed and concentration oscillations of the targeted species are deduced from the equations of chemical kinetics. We determine analytical expressions of the resonance conditions on the control parameters, angular frequency, mean light intensity, and total concentration of the photoswitchable reagent, which optimize the out-of-phase amplitude of concentration oscillations. A user-friendly protocol of dynamic titration is proposed. © the Owner Societies. |
Macroscale fluorescence imaging against autofluorescence under ambient light Article de journal R Zhang; R Chouket; M -A Plamont; Z Kelemen; A Espagne; A G Tebo; A Gautier; L Gissot; J -D Faure; L Jullien; V Croquette; T Le Saux Light: Science and Applications, 7 (1), 2018. @article{Zhang:2018c, title = {Macroscale fluorescence imaging against autofluorescence under ambient light}, author = {R Zhang and R Chouket and M -A Plamont and Z Kelemen and A Espagne and A G Tebo and A Gautier and L Gissot and J -D Faure and L Jullien and V Croquette and T Le Saux}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85058077105&doi=10.1038%2fs41377-018-0098-6&partnerID=40&md5=958a21f219ba413e687d1860d21c2767}, doi = {10.1038/s41377-018-0098-6}, year = {2018}, date = {2018-01-01}, journal = {Light: Science and Applications}, volume = {7}, number = {1}, abstract = {Macroscale fluorescence imaging is increasingly used to observe biological samples. However, it may suffer from spectral interferences that originate from ambient light or autofluorescence of the sample or its support. In this manuscript, we built a simple and inexpensive fluorescence macroscope, which has been used to evaluate the performance of Speed OPIOM (Out of Phase Imaging after Optical Modulation), which is a reference-free dynamic contrast protocol, to selectively image reversibly photoswitchable fluorophores as labels against detrimental autofluorescence and ambient light. By tuning the intensity and radial frequency of the modulated illumination to the Speed OPIOM resonance and adopting a phase-sensitive detection scheme that ensures noise rejection, we enhanced the sensitivity and the signal-to-noise ratio for fluorescence detection in blot assays by factors of 50 and 10, respectively, over direct fluorescence observation under constant illumination. Then, we overcame the strong autofluorescence of growth media that are currently used in microbiology and realized multiplexed fluorescence observation of colonies of spectrally similar fluorescent bacteria with a unique configuration of excitation and emission wavelengths. Finally, we easily discriminated fluorescent labels from the autofluorescent and reflective background in labeled leaves, even under the interference of incident light at intensities that are comparable to sunlight. The proposed approach is expected to find multiple applications, from biological assays to outdoor observations, in fluorescence macroimaging. © 2018, The Author(s).}, keywords = {}, pubstate = {published}, tppubtype = {article} } Macroscale fluorescence imaging is increasingly used to observe biological samples. However, it may suffer from spectral interferences that originate from ambient light or autofluorescence of the sample or its support. In this manuscript, we built a simple and inexpensive fluorescence macroscope, which has been used to evaluate the performance of Speed OPIOM (Out of Phase Imaging after Optical Modulation), which is a reference-free dynamic contrast protocol, to selectively image reversibly photoswitchable fluorophores as labels against detrimental autofluorescence and ambient light. By tuning the intensity and radial frequency of the modulated illumination to the Speed OPIOM resonance and adopting a phase-sensitive detection scheme that ensures noise rejection, we enhanced the sensitivity and the signal-to-noise ratio for fluorescence detection in blot assays by factors of 50 and 10, respectively, over direct fluorescence observation under constant illumination. Then, we overcame the strong autofluorescence of growth media that are currently used in microbiology and realized multiplexed fluorescence observation of colonies of spectrally similar fluorescent bacteria with a unique configuration of excitation and emission wavelengths. Finally, we easily discriminated fluorescent labels from the autofluorescent and reflective background in labeled leaves, even under the interference of incident light at intensities that are comparable to sunlight. The proposed approach is expected to find multiple applications, from biological assays to outdoor observations, in fluorescence macroimaging. © 2018, The Author(s). |
Mixed Copolymer Adlayers Allowing Reversible Thermal Control of Single Cell Aspect Ratio Article de journal F Dalier; G V Dubacheva; M Coniel; D Zanchi; A Galtayries; M Piel; E Marie; C Tribet Acs Applied Materials & Interfaces, 10 (3), p. 2253-2258, 2018, ISSN: 1944-8244. @article{Dalier:2018, title = {Mixed Copolymer Adlayers Allowing Reversible Thermal Control of Single Cell Aspect Ratio}, author = {F Dalier and G V Dubacheva and M Coniel and D Zanchi and A Galtayries and M Piel and E Marie and C Tribet}, doi = {10.1021/acsami.7b18513}, issn = {1944-8244}, year = {2018}, date = {2018-01-01}, journal = {Acs Applied Materials & Interfaces}, volume = {10}, number = {3}, pages = {2253-2258}, abstract = {Dynamic guidance of living cells is achieved by fine-tuning and spatiotemporal modulation on artificial polymer layers enabling reversible peptide display. Adjustment of surface composition and interactions is obtained by coadsorption of mixed poly(lysine) derivatives, grafted with either repellent PEG, RGD adhesion peptides, or T-responsive poly(N-isopropylacrylamide) strands. Deposition of mixed adlayers provides a straightforward mean to optimize complex substrates, which is here implemented to achieve (1) thermal control of ligand accessibility and (2) adjustment of relative adhesiveness between adjacent micropatterns, while preserving cell attachment during thermal cycles. The reversible polarization of HeLa cells along orthogonal stripes mimics guidance along natural matrices.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dynamic guidance of living cells is achieved by fine-tuning and spatiotemporal modulation on artificial polymer layers enabling reversible peptide display. Adjustment of surface composition and interactions is obtained by coadsorption of mixed poly(lysine) derivatives, grafted with either repellent PEG, RGD adhesion peptides, or T-responsive poly(N-isopropylacrylamide) strands. Deposition of mixed adlayers provides a straightforward mean to optimize complex substrates, which is here implemented to achieve (1) thermal control of ligand accessibility and (2) adjustment of relative adhesiveness between adjacent micropatterns, while preserving cell attachment during thermal cycles. The reversible polarization of HeLa cells along orthogonal stripes mimics guidance along natural matrices. |
Modifying the Steric Properties in the Second Coordination Sphere of Designed Peptides Leads to Enhancement of Nitrite Reductase Activity Article de journal Karl J Koebke; Fangting Yu; Elvin Salerno; Casey Van Stappen; Alison G Tebo; James E Penner-Hahn; Vincent L Pecoraro Angewandte Chemie International Edition, 57 (15), p. 3954-3957, 2018. @article{Koebke:2018a, title = {Modifying the Steric Properties in the Second Coordination Sphere of Designed Peptides Leads to Enhancement of Nitrite Reductase Activity}, author = {Karl J Koebke and Fangting Yu and Elvin Salerno and Casey Van Stappen and Alison G Tebo and James E {Penner-Hahn} and Vincent L Pecoraro}, doi = {10.1002/anie.201712757}, year = {2018}, date = {2018-01-01}, journal = {Angewandte Chemie International Edition}, volume = {57}, number = {15}, pages = {3954-3957}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Redox switchable rhodamine-ferrocene dyad: Exploring imaging possibilities in cells Article de journal M Čížková; L Cattiaux; J Pandard; M Guille-Collignon; F Lemaître; J Delacotte; J -M Mallet; E Labbé; O Buriez Electrochemistry Communications, 97 , p. 46–50, 2018. @article{Cizkova:2018, title = {Redox switchable rhodamine-ferrocene dyad: Exploring imaging possibilities in cells}, author = {M \v{C}\'{i}\v{z}kov\'{a} and L Cattiaux and J Pandard and M Guille-Collignon and F Lema\^{i}tre and J Delacotte and J -M Mallet and E Labb\'{e} and O Buriez}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85054592456&doi=10.1016%2fj.elecom.2018.10.009&partnerID=40&md5=10a4aed1c89bb6a788a2a260bbd0a818}, doi = {10.1016/j.elecom.2018.10.009}, year = {2018}, date = {2018-01-01}, journal = {Electrochemistry Communications}, volume = {97}, pages = {46--50}, abstract = {An original redox-responsive fluorescent probe combining a rhodamine derivative and a ferrocenyl moiety used as the fluorescence modulator was designed, synthesized and characterized. The fluorescence of this new dyad could be tuned from the redox state of ferrocene, a feature observed both electrochemically and on cancer cells incubated with this probe. © 2018 Elsevier B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } An original redox-responsive fluorescent probe combining a rhodamine derivative and a ferrocenyl moiety used as the fluorescence modulator was designed, synthesized and characterized. The fluorescence of this new dyad could be tuned from the redox state of ferrocene, a feature observed both electrochemically and on cancer cells incubated with this probe. © 2018 Elsevier B.V. |
Rolling and aging in temperature-ramp soft adhesion Article de journal G Boniello; C Tribet; E Marie; V Croquette; D Zanchi Physical Review E, 97 (1), 2018. @article{Boniello:2018, title = {Rolling and aging in temperature-ramp soft adhesion}, author = {G Boniello and C Tribet and E Marie and V Croquette and D Zanchi}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040729302&doi=10.1103%2fPhysRevE.97.012609&partnerID=40&md5=5b4fb855a186c8f56070f77a7d75f5c8}, doi = {10.1103/PhysRevE.97.012609}, year = {2018}, date = {2018-01-01}, journal = {Physical Review E}, volume = {97}, number = {1}, abstract = {Immediately before adsorption to a horizontal substrate, sinking polymer-coated colloids can undergo a complex sequence of landing, jumping, crawling, and rolling events. Using video tracking, we studied the soft adhesion to a horizontal flat plate of micron-size colloids coated by a controlled molar fraction f of the poly(lysine)-grafted-poly(N-isopropylacrylamide) (PLL-g-PNIPAM) which is a temperature-sensitive polymer. We ramp the temperature from below to above Tc=32±1C, at which the PNIPAM polymer undergoes a transition, triggering attractive interaction between microparticles and surface. The adsorption rate, the effective in-plane (x-y) diffusion constant, and the average residence time distribution over z were extracted from the Brownian motion records during last seconds before immobilization. Experimental data are understood within a rate-equations-based model that includes aging effects and includes three populations: the untethered, the rolling, and the arrested colloids. We show that preadsorption dynamics casts a characteristic scaling function α(f) proportional to the number of available PNIPAM patches met by soft contact during Brownian rolling. In particular, the increase of in-plane diffusivity with increasing f is understood: The stickiest particles have the shortest rolling regime prior to arrest, so that their motion is dominated by the untethered phase. © 2018 American Physical Society.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Immediately before adsorption to a horizontal substrate, sinking polymer-coated colloids can undergo a complex sequence of landing, jumping, crawling, and rolling events. Using video tracking, we studied the soft adhesion to a horizontal flat plate of micron-size colloids coated by a controlled molar fraction f of the poly(lysine)-grafted-poly(N-isopropylacrylamide) (PLL-g-PNIPAM) which is a temperature-sensitive polymer. We ramp the temperature from below to above Tc=32±1C, at which the PNIPAM polymer undergoes a transition, triggering attractive interaction between microparticles and surface. The adsorption rate, the effective in-plane (x-y) diffusion constant, and the average residence time distribution over z were extracted from the Brownian motion records during last seconds before immobilization. Experimental data are understood within a rate-equations-based model that includes aging effects and includes three populations: the untethered, the rolling, and the arrested colloids. We show that preadsorption dynamics casts a characteristic scaling function α(f) proportional to the number of available PNIPAM patches met by soft contact during Brownian rolling. In particular, the increase of in-plane diffusivity with increasing f is understood: The stickiest particles have the shortest rolling regime prior to arrest, so that their motion is dominated by the untethered phase. © 2018 American Physical Society. |
Spying on cells with chemical-genetic hybrids Article de journal A Gautier Actualite Chimique, (435), p. 31–35, 2018. @article{Gautier:2018b, title = {Spying on cells with chemical-genetic hybrids}, author = {A Gautier}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85059310973&partnerID=40&md5=89fdbcf594a9f3d4fe3ad8b1507ccef9}, year = {2018}, date = {2018-01-01}, journal = {Actualite Chimique}, number = {435}, pages = {31--35}, abstract = {Cells and organisms are complex machines regulated by a set of dynamic events orchestrated in space and time. Our understanding of their inner workings is intimately linked to our ability to observe how their constituents organize and interact. Optical microscopy allows to observe living systems at submicrometric scale. The development of high-performance fluorescent markers makes possible nowadays to monitor the dynamics of biomolecules with an unprecedented spatial and temporal resolution. This article presents how chemistry and biology can team up to develop next-generation markers that push the boundaries of biological imaging. © 2018 Societe Francaise de Chimie. All rights reserved.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Cells and organisms are complex machines regulated by a set of dynamic events orchestrated in space and time. Our understanding of their inner workings is intimately linked to our ability to observe how their constituents organize and interact. Optical microscopy allows to observe living systems at submicrometric scale. The development of high-performance fluorescent markers makes possible nowadays to monitor the dynamics of biomolecules with an unprecedented spatial and temporal resolution. This article presents how chemistry and biology can team up to develop next-generation markers that push the boundaries of biological imaging. © 2018 Societe Francaise de Chimie. All rights reserved. |
The Inducible Chemical-Genetic Fluorescent Marker FAST Outperforms Classical Fluorescent Proteins in the Quantitative Reporting of Bacterial Biofilm Dynamics Article de journal Amaury Monmeyran; Philippe Thomen; Hugo Jonqui`ere; Franck Sureau; Chenge Li; Marie-Aude Plamont; Carine Douarche; Jean-Franc cois Casella; Arnaud Gautier; Nelly Henry Scientific Reports, 8 (1), p. 10336, 2018, ISSN: 2045-2322. @article{RN36b, title = {The Inducible Chemical-Genetic Fluorescent Marker FAST Outperforms Classical Fluorescent Proteins in the Quantitative Reporting of Bacterial Biofilm Dynamics}, author = {Amaury Monmeyran and Philippe Thomen and Hugo Jonqui{`e}re and Franck Sureau and Chenge Li and Marie-Aude Plamont and Carine Douarche and Jean-Fran{c c}ois Casella and Arnaud Gautier and Nelly Henry}, doi = {10.1038/s41598-018-28643-z}, issn = {2045-2322}, year = {2018}, date = {2018-01-01}, journal = {Scientific Reports}, volume = {8}, number = {1}, pages = {10336}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2017 |
A Dual Functional Electroactive and Fluorescent Probe for Coupled Measurements of Vesicular Exocytosis with High Spatial and Temporal Resolution Article de journal X Liu; A Savy; S Maurin; L Grimaud; F Darchen; D Quinton; E Labbé; O Buriez; J Delacotte; F Lemaître; M Guille-Collignon Angewandte Chemie - International Edition, 56 (9), p. 2366–2370, 2017. @article{Liu:2017a, title = {A Dual Functional Electroactive and Fluorescent Probe for Coupled Measurements of Vesicular Exocytosis with High Spatial and Temporal Resolution}, author = {X Liu and A Savy and S Maurin and L Grimaud and F Darchen and D Quinton and E Labb\'{e} and O Buriez and J Delacotte and F Lema\^{i}tre and M Guille-Collignon}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85010696856&doi=10.1002%2fanie.201611145&partnerID=40&md5=a51767157166d7f185f0195a28b347b8}, doi = {10.1002/anie.201611145}, year = {2017}, date = {2017-01-01}, journal = {Angewandte Chemie - International Edition}, volume = {56}, number = {9}, pages = {2366--2370}, abstract = {In this work, Fluorescent False Neurotransmitter 102 (FFN102), a synthesized analogue of biogenic neurotransmitters, was demonstrated to show both pH-dependent fluorescence and electroactivity. To study secretory behaviors at the single-vesicle level, FFN102 was employed as a new fluorescent/electroactive dual probe in a coupled technique (amperometry and total internal reflection fluorescence microscopy (TIRFM)). We used N13 cells, a stable clone of BON cells, to specifically accumulate FFN102 into their secretory vesicles, and then optical and electrochemical measurements of vesicular exocytosis were experimentally achieved by using indium tin oxide (ITO) transparent electrodes. Upon stimulation, FFN102 started to diffuse out from the acidic intravesicular microenvironment to the neutral extracellular space, leading to fluorescent emissions and to the electrochemical oxidation signals that were simultaneously collected from the ITO electrode surface. The correlation of fluorescence and amperometric signals resulting from the FFN102 probe allows real-time monitoring of single exocytotic events with both high spatial and temporal resolution. This work opens new possibilities in the investigation of exocytotic mechanisms. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim}, keywords = {}, pubstate = {published}, tppubtype = {article} } In this work, Fluorescent False Neurotransmitter 102 (FFN102), a synthesized analogue of biogenic neurotransmitters, was demonstrated to show both pH-dependent fluorescence and electroactivity. To study secretory behaviors at the single-vesicle level, FFN102 was employed as a new fluorescent/electroactive dual probe in a coupled technique (amperometry and total internal reflection fluorescence microscopy (TIRFM)). We used N13 cells, a stable clone of BON cells, to specifically accumulate FFN102 into their secretory vesicles, and then optical and electrochemical measurements of vesicular exocytosis were experimentally achieved by using indium tin oxide (ITO) transparent electrodes. Upon stimulation, FFN102 started to diffuse out from the acidic intravesicular microenvironment to the neutral extracellular space, leading to fluorescent emissions and to the electrochemical oxidation signals that were simultaneously collected from the ITO electrode surface. The correlation of fluorescence and amperometric signals resulting from the FFN102 probe allows real-time monitoring of single exocytotic events with both high spatial and temporal resolution. This work opens new possibilities in the investigation of exocytotic mechanisms. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim |
Chromophore Renewal and Fluorogen-Binding Tags: A Match Made to Last Article de journal Frederico M Pimenta; Giovanni Chiappetta; Thomas Le Saux; Jo"elle Vinh; Ludovic Jullien; Arnaud Gautier Scientific Reports, 7 (1), p. 12316, 2017, ISSN: 2045-2322. @article{RN35b, title = {Chromophore Renewal and Fluorogen-Binding Tags: A Match Made to Last}, author = {Frederico M Pimenta and Giovanni Chiappetta and Thomas Le Saux and Jo{"e}lle Vinh and Ludovic Jullien and Arnaud Gautier}, doi = {10.1038/s41598-017-12400-9}, issn = {2045-2322}, year = {2017}, date = {2017-01-01}, journal = {Scientific Reports}, volume = {7}, number = {1}, pages = {12316}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Determination of the R(−) and S(+)-enantiomers of vigabatrin in human plasma by ultra-high-performance liquid chromatography and tandem mass-spectrometry Article de journal P Duhamel; M Ounissi; T Le Saux; H Bienayme; C Chiron; V Jullien Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 1070 , p. 31–36, 2017. @article{Duhamel:2017, title = {Determination of the R(−) and S(+)-enantiomers of vigabatrin in human plasma by ultra-high-performance liquid chromatography and tandem mass-spectrometry}, author = {P Duhamel and M Ounissi and T Le Saux and H Bienayme and C Chiron and V Jullien}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85032328645&doi=10.1016%2fj.jchromb.2017.10.037&partnerID=40&md5=29a0659d0b0d1d6916a39f43941aae79}, doi = {10.1016/j.jchromb.2017.10.037}, year = {2017}, date = {2017-01-01}, journal = {Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences}, volume = {1070}, pages = {31--36}, abstract = {An analytical method was developed for the quantification in plasma of the R and S enantiomers of vigabatrin (VGB), a drug used for the treatment of some refractory pediatric epileptic syndromes. After adding 50 μL of the internal standard, which consisted of a 15 mg/L solution of deuterated racemic VGB, and 100 μL of water to 100 μL of plasma samples, a protein precipitation was performed by adding 600 μL of methanol. The supernatant was evaporated to dryness under a stream of nitrogen and the dry residue was reconstituted with 500 μL of water. Then, 100 μL of 0.01 M o-phthaldialdehyde and 0.01 M N-acetyl-L-cysteine in borate buffer (0.1 M}, keywords = {}, pubstate = {published}, tppubtype = {article} } An analytical method was developed for the quantification in plasma of the R and S enantiomers of vigabatrin (VGB), a drug used for the treatment of some refractory pediatric epileptic syndromes. After adding 50 μL of the internal standard, which consisted of a 15 mg/L solution of deuterated racemic VGB, and 100 μL of water to 100 μL of plasma samples, a protein precipitation was performed by adding 600 μL of methanol. The supernatant was evaporated to dryness under a stream of nitrogen and the dry residue was reconstituted with 500 μL of water. Then, 100 μL of 0.01 M o-phthaldialdehyde and 0.01 M N-acetyl-L-cysteine in borate buffer (0.1 M |
Discovery of Chemoautotrophic Symbiosis in the Giant Shipworm Kuphus Polythalamia(Bivalvia: Teredinidae) Extends Wooden-Steps Theory Article de journal Daniel L Distel; Marvin A Altamia; Zhenjian Lin; Reuben J Shipway; Andrew Han; Imelda Forteza; Rowena Antemano; Ma Gwen Pe~naflor J Limbaco; Alison G Tebo; Rande Dechavez; Julie Albano; Gary Rosenberg; Gisela P Concepcion; Eric W Schmidt; Margo G Haygood Proceedings of the National Academy of Sciences, 114 (18), p. E3652-E3658, 2017. @article{Distel:2017, title = {Discovery of Chemoautotrophic Symbiosis in the Giant Shipworm Kuphus Polythalamia(Bivalvia: Teredinidae) Extends Wooden-Steps Theory}, author = {Daniel L Distel and Marvin A Altamia and Zhenjian Lin and Reuben J Shipway and Andrew Han and Imelda Forteza and Rowena Antemano and Ma Gwen Pe{~n}aflor J Limbaco and Alison G Tebo and Rande Dechavez and Julie Albano and Gary Rosenberg and Gisela P Concepcion and Eric W Schmidt and Margo G Haygood}, doi = {10.1073/pnas.1620470114}, year = {2017}, date = {2017-05-01}, journal = {Proceedings of the National Academy of Sciences}, volume = {114}, number = {18}, pages = {E3652-E3658}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Dynamic multicolor protein labeling in living cells Article de journal C Li; M -A Plamont; H L Sladitschek; V Rodrigues; I Aujard; P Neveu; T Le Saux; L Jullien; A Gautier Chemical Science, 8 (8), p. 5598–5605, 2017. @article{Li:2017a, title = {Dynamic multicolor protein labeling in living cells}, author = {C Li and M -A Plamont and H L Sladitschek and V Rodrigues and I Aujard and P Neveu and T Le Saux and L Jullien and A Gautier}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85023627118&doi=10.1039%2fc7sc01364g&partnerID=40&md5=dee65e052a12f416631a2699edfa5dd1}, doi = {10.1039/c7sc01364g}, year = {2017}, date = {2017-01-01}, journal = {Chemical Science}, volume = {8}, number = {8}, pages = {5598--5605}, abstract = {Yellow Fluorescence-Activating and absorption-Shifting Tag (Y-FAST, hereafter called FAST) is a 14 kDa protein tag giving a bright green-yellow fluorescent complex upon interaction with the fluorogenic dye 4-hydroxy-3-methylbenzylidene rhodanine (HMBR). Here, we report a collection of fluorogens enabling tuning of the fluorescence color of FAST from green-yellow to orange and red. Beyond allowing the multicolor imaging of FAST-tagged proteins in live cells, these fluorogens enable dynamic color switching because of FAST's reversible labeling. This unprecedented behavior allows for selective detection of FAST-tagged proteins in cells expressing both green and red fluorescent species through two-color cross-correlation, opening up exciting prospects to overcome spectral crowding and push the frontiers of multiplexed imaging. © 2017 The Royal Society of Chemistry.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Yellow Fluorescence-Activating and absorption-Shifting Tag (Y-FAST, hereafter called FAST) is a 14 kDa protein tag giving a bright green-yellow fluorescent complex upon interaction with the fluorogenic dye 4-hydroxy-3-methylbenzylidene rhodanine (HMBR). Here, we report a collection of fluorogens enabling tuning of the fluorescence color of FAST from green-yellow to orange and red. Beyond allowing the multicolor imaging of FAST-tagged proteins in live cells, these fluorogens enable dynamic color switching because of FAST's reversible labeling. This unprecedented behavior allows for selective detection of FAST-tagged proteins in cells expressing both green and red fluorescent species through two-color cross-correlation, opening up exciting prospects to overcome spectral crowding and push the frontiers of multiplexed imaging. © 2017 The Royal Society of Chemistry. |
G Longatte; M Guille-Collignon; F Lemaître ChemPhysChem, 18 (19), p. 2643–2650, 2017. @article{Longatte:2017a, title = {Electrocatalytic Mechanism Involving Michaelis\textendashMenten Kinetics at the Preparative Scale: Theory and Applicability to Photocurrents from a Photosynthetic Algae Suspension With Quinones}, author = {G Longatte and M Guille-Collignon and F Lema\^{i}tre}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028605961&doi=10.1002%2fcphc.201700351&partnerID=40&md5=0500ecaa88d980132140883a725bedfb}, doi = {10.1002/cphc.201700351}, year = {2017}, date = {2017-01-01}, journal = {ChemPhysChem}, volume = {18}, number = {19}, pages = {2643--2650}, abstract = {In the past years, many strategies have been implemented to benefit from oxygenic photosynthesis to harvest photosynthetic electrons and produce a significant photocurrent. Therefore, electrochemical tools were considered and have globally relied on the electron transfer(s) between the photosynthetic chain and a collecting electrode. In this context, we recently reported the implementation of an electrochemical set-up at the preparative scale to produce photocurrents from a Chlamydomonas reinhardtii algae suspension with an appropriate mediator (2,6-DCBQ) and a carbon gauze as the working electrode. In the present work, we wish to describe a mathematical modeling of the recorded photocurrents to better understand the effects of the experimental conditions on the photosynthetic extraction of electrons. In that way, we established a general model of an electrocatalytic mechanism at the preparative scale (that is, assuming a homogenous bulk solution at any time and a constant diffusion layer, both assumptions being valid under forced convection) in which the chemical step involves a Michaelis\textendashMenten-like behaviour. Dependences of transient and steady-state corresponding currents were analysed as a function of different parameters by means of zone diagrams. This model was tested to our experimental data related to photosynthesis. The corresponding results suggest that competitive pathways beyond photosynthetic harvesting alone should be taken into account. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim}, keywords = {}, pubstate = {published}, tppubtype = {article} } In the past years, many strategies have been implemented to benefit from oxygenic photosynthesis to harvest photosynthetic electrons and produce a significant photocurrent. Therefore, electrochemical tools were considered and have globally relied on the electron transfer(s) between the photosynthetic chain and a collecting electrode. In this context, we recently reported the implementation of an electrochemical set-up at the preparative scale to produce photocurrents from a Chlamydomonas reinhardtii algae suspension with an appropriate mediator (2,6-DCBQ) and a carbon gauze as the working electrode. In the present work, we wish to describe a mathematical modeling of the recorded photocurrents to better understand the effects of the experimental conditions on the photosynthetic extraction of electrons. In that way, we established a general model of an electrocatalytic mechanism at the preparative scale (that is, assuming a homogenous bulk solution at any time and a constant diffusion layer, both assumptions being valid under forced convection) in which the chemical step involves a Michaelis–Menten-like behaviour. Dependences of transient and steady-state corresponding currents were analysed as a function of different parameters by means of zone diagrams. This model was tested to our experimental data related to photosynthesis. The corresponding results suggest that competitive pathways beyond photosynthetic harvesting alone should be taken into account. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim |
Electrochemical Characterization of Plutonium in n-Tributyl Phosphate Article de journal Mohamed Ali Bahri; Alexandre Ruas; Eric Labbé; Philippe Moisy Dalton Transactions, p. 4943-4949, 2017. @article{RID:0519170943437-66b, title = {Electrochemical Characterization of Plutonium in n-Tributyl Phosphate}, author = {Mohamed Ali Bahri and Alexandre Ruas and Eric Labb\'{e} and Philippe Moisy}, year = {2017}, date = {2017-01-01}, journal = {Dalton Transactions}, pages = {4943-4949}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Electrochemical Harvesting of Photosynthetic Electrons from Unicellular Algae Population at the Preparative Scale by Using 2,6-dichlorobenzoquinone Article de journal G Longatte; F Rappaport; F -A Wollman; M Guille-Collignon; F Lemaître Electrochimica Acta, 236 , p. 337–342, 2017. @article{Longatte:2017, title = {Electrochemical Harvesting of Photosynthetic Electrons from Unicellular Algae Population at the Preparative Scale by Using 2,6-dichlorobenzoquinone}, author = {G Longatte and F Rappaport and F -A Wollman and M Guille-Collignon and F Lema\^{i}tre}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85016504359&doi=10.1016%2fj.electacta.2017.03.124&partnerID=40&md5=d8a7614ce4f287a9f115d922ab5ee8f6}, doi = {10.1016/j.electacta.2017.03.124}, year = {2017}, date = {2017-01-01}, journal = {Electrochimica Acta}, volume = {236}, pages = {337--342}, abstract = {Oxygenic photosynthesis is the process used by plants, cyanobacteria or algae to convert the solar energy into a chemical one from the carbon dioxide reduction and water oxidation. In the past years, many strategies were implemented to take benefits from the overall low yield of this process to extract photosynthetic electrons and thus produce a sustainable photocurrent. In practice, electrochemical tools were involved and the principle of electrons harvestings was related to the step of electron transfer between the photosynthetic organism and a collecting electrode. In this context, works involving an algae population in suspension were rather scarce and rather focus on the grafting of the photosynthetic machinery at the electrode surface. Based on our previous works, we report here the implementation of an electrochemical set-up at the preparative scale to produce photocurrents. An algae suspension, i.e. an intact biological system to ensure culture and growth, was involved in presence of a centimeter-sized carbon gauze as the collecting electrode. The spectroelectrochemical cell contains 16 mL of suspension of a Chlamydomonas reinhardtii mutant with an appropriate mediator (2,6-DCBQ). Under these conditions, stable photocurrents were recorded over 1 h whose magnitude depends on the quinone concentration and the light illumination. © 2017 Elsevier Ltd}, keywords = {}, pubstate = {published}, tppubtype = {article} } Oxygenic photosynthesis is the process used by plants, cyanobacteria or algae to convert the solar energy into a chemical one from the carbon dioxide reduction and water oxidation. In the past years, many strategies were implemented to take benefits from the overall low yield of this process to extract photosynthetic electrons and thus produce a sustainable photocurrent. In practice, electrochemical tools were involved and the principle of electrons harvestings was related to the step of electron transfer between the photosynthetic organism and a collecting electrode. In this context, works involving an algae population in suspension were rather scarce and rather focus on the grafting of the photosynthetic machinery at the electrode surface. Based on our previous works, we report here the implementation of an electrochemical set-up at the preparative scale to produce photocurrents. An algae suspension, i.e. an intact biological system to ensure culture and growth, was involved in presence of a centimeter-sized carbon gauze as the collecting electrode. The spectroelectrochemical cell contains 16 mL of suspension of a Chlamydomonas reinhardtii mutant with an appropriate mediator (2,6-DCBQ). Under these conditions, stable photocurrents were recorded over 1 h whose magnitude depends on the quinone concentration and the light illumination. © 2017 Elsevier Ltd |
R de Oliveira; M Durand; L Challier; P Messina; J M Swiecicki; M Di Pisa; G Chassaing; S Lavielle; O Buriez; E Labbé Journal of Electroanalytical Chemistry, 788 , p. 225–231, 2017. @article{deOliveira:2017, title = {Electrochemical quenching of the fluorescence produced by NBD-labelled cell penetrating peptides: A contribution to the study of their internalization in large unilamellar vesicles}, author = {R de Oliveira and M Durand and L Challier and P Messina and J M Swiecicki and M Di Pisa and G Chassaing and S Lavielle and O Buriez and E Labb\'{e}}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85013176758&doi=10.1016%2fj.jelechem.2017.02.006&partnerID=40&md5=725e8c07b1f4090ecf4f2ceabb57e6f7}, doi = {10.1016/j.jelechem.2017.02.006}, year = {2017}, date = {2017-01-01}, journal = {Journal of Electroanalytical Chemistry}, volume = {788}, pages = {225--231}, abstract = {This work investigates the implementation of a simple and versatile electrochemical setup aimed at achieving a fast and complete fluorescence extinction of NBD-labelled (NBD = 7-nitrobenz-2-oxa-1,3-diazole) cell penetrating peptides contained in 2\textendash5 cm3 samples containing phosphate buffer + large unilamellar vesicles. The quenching is obtained through a reductive electrolysis in a 2-compartment cell homebuilt from disposable plastic labware, which remains inert towards the adsorption of both peptides and lipid vesicles. Considering the micromolar concentration of NBD-tagged peptides, the main electrochemical reaction observed is hydrogen evolution, NBD reduction representing a small fraction of the cathodic current/charge engaged. The electrolysis conditions are discussed with respect to the nature of the reduction products formed, the integrity of large unilamellar vesicles and phosphate buffering properties. This electrochemical method is compared to the traditional chemical dithionite quenching of NBD and tested to monitor the internalization of cell penetrating peptides in large unilamellar vesicles. © 2017 Elsevier B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This work investigates the implementation of a simple and versatile electrochemical setup aimed at achieving a fast and complete fluorescence extinction of NBD-labelled (NBD = 7-nitrobenz-2-oxa-1,3-diazole) cell penetrating peptides contained in 2–5 cm3 samples containing phosphate buffer + large unilamellar vesicles. The quenching is obtained through a reductive electrolysis in a 2-compartment cell homebuilt from disposable plastic labware, which remains inert towards the adsorption of both peptides and lipid vesicles. Considering the micromolar concentration of NBD-tagged peptides, the main electrochemical reaction observed is hydrogen evolution, NBD reduction representing a small fraction of the cathodic current/charge engaged. The electrolysis conditions are discussed with respect to the nature of the reduction products formed, the integrity of large unilamellar vesicles and phosphate buffering properties. This electrochemical method is compared to the traditional chemical dithionite quenching of NBD and tested to monitor the internalization of cell penetrating peptides in large unilamellar vesicles. © 2017 Elsevier B.V. |
Erratum: Author Correction: Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations (Nature communications (2017) 8 1 (969)) Article de journal J Quérard; R Zhang; Z Kelemen; M -A Plamont; X Xie; R Chouket; I Roemgens; Y Korepina; S Albright; E Ipendey; M Volovitch; H L Sladitschek; P Neveu; L Gissot; A Gautier; J -D Faure; V Croquette; T Le Saux; L Jullien Nature communications, 8 (1), p. 2173, 2017. @article{Querard:2017, title = {Erratum: Author Correction: Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations (Nature communications (2017) 8 1 (969))}, author = {J Qu\'{e}rard and R Zhang and Z Kelemen and M -A Plamont and X Xie and R Chouket and I Roemgens and Y Korepina and S Albright and E Ipendey and M Volovitch and H L Sladitschek and P Neveu and L Gissot and A Gautier and J -D Faure and V Croquette and T Le Saux and L Jullien}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85058747083&doi=10.1038%2fs41467-017-02133-8&partnerID=40&md5=15b4153817b7bd74d9691a4083989504}, doi = {10.1038/s41467-017-02133-8}, year = {2017}, date = {2017-01-01}, journal = {Nature communications}, volume = {8}, number = {1}, pages = {2173}, abstract = {The Peer Review File associated with this Article was updated shortly after publication to redact from the authors' point-by-point response a description of unpublished work describing how Speed OPIOM may in future be used to facilitate discrimination between FRET and direct excitation.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The Peer Review File associated with this Article was updated shortly after publication to redact from the authors' point-by-point response a description of unpublished work describing how Speed OPIOM may in future be used to facilitate discrimination between FRET and direct excitation. |
Fluorogenic labeling strategies for biological imaging Article de journal C Li; A G Tebo; A Gautier International Journal of Molecular Sciences, 18 (7), 2017. @article{Li:2017b, title = {Fluorogenic labeling strategies for biological imaging}, author = {C Li and A G Tebo and A Gautier}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85023628010&doi=10.3390%2fijms18071473&partnerID=40&md5=d7fc35685b4a7cb4def13c1a52a66d92}, doi = {10.3390/ijms18071473}, year = {2017}, date = {2017-01-01}, journal = {International Journal of Molecular Sciences}, volume = {18}, number = {7}, abstract = {The spatiotemporal fluorescence imaging of biological processes requires effective tools to label intracellular biomolecules in living systems. This review presents a brief overview of recent labeling strategies that permits one to make protein and RNA strongly fluorescent using synthetic fluorogenic probes. Genetically encoded tags selectively binding the exogenously applied molecules ensure high labeling selectivity, while high imaging contrast is achieved using fluorogenic chromophores that are fluorescent only when bound to their cognate tag, and are otherwise dark. Beyond avoiding the need for removal of unbound synthetic dyes, these approaches allow the development of sophisticated imaging assays, and open exciting prospects for advanced imaging, particularly for multiplexed imaging and super-resolution microscopy. © 2017 by the authors. Licensee MDPI, Basel, Switzerland.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The spatiotemporal fluorescence imaging of biological processes requires effective tools to label intracellular biomolecules in living systems. This review presents a brief overview of recent labeling strategies that permits one to make protein and RNA strongly fluorescent using synthetic fluorogenic probes. Genetically encoded tags selectively binding the exogenously applied molecules ensure high labeling selectivity, while high imaging contrast is achieved using fluorogenic chromophores that are fluorescent only when bound to their cognate tag, and are otherwise dark. Beyond avoiding the need for removal of unbound synthetic dyes, these approaches allow the development of sophisticated imaging assays, and open exciting prospects for advanced imaging, particularly for multiplexed imaging and super-resolution microscopy. © 2017 by the authors. Licensee MDPI, Basel, Switzerland. |
Hybrid fluorescent probes for imaging cellular proteins on demand Article de journal L Jullien; A Gautier Medecine/Sciences, 33 (6-7), p. 576–578, 2017. @article{Jullien:2017, title = {Hybrid fluorescent probes for imaging cellular proteins on demand}, author = {L Jullien and A Gautier}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85044173814&doi=10.1051%2fmedsci%2f20173306006&partnerID=40&md5=438f0ceb949779e8a90a779a37a1d890}, doi = {10.1051/medsci/20173306006}, year = {2017}, date = {2017-01-01}, journal = {Medecine/Sciences}, volume = {33}, number = {6-7}, pages = {576--578}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Indium Tin Oxide Microsystem for Electrochemical Detection of Exocytosis of Migratory Dendritic Cells Article de journal X Liu; M Bretou; A -M Lennon-Duménil; F Lemaître; M Guille-Collignon Electroanalysis, 29 (1), p. 197–202, 2017. @article{Liu:2017b, title = {Indium Tin Oxide Microsystem for Electrochemical Detection of Exocytosis of Migratory Dendritic Cells}, author = {X Liu and M Bretou and A -M Lennon-Dum\'{e}nil and F Lema\^{i}tre and M Guille-Collignon}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84992456261&doi=10.1002%2felan.201600360&partnerID=40&md5=dbe0bcd2017bc2da52f267117aab382f}, doi = {10.1002/elan.201600360}, year = {2017}, date = {2017-01-01}, journal = {Electroanalysis}, volume = {29}, number = {1}, pages = {197--202}, abstract = {The design, fabrication and test of an indium tin oxide (ITO) microdevice to investigate exocytotic behaviors of migratory dendritic cells (DCs) in confined three-dimensional environment were reported in this work. Indeed, immature DCs were able to migrate into micro-fabricated biocompatible polydimethylsiloxane (PDMS) channels that mimic their natural constrained environment of tissues for patrolling in search of danger associated antigens through an endocytotic process called macropinocytosis. In order to coordinate membrane trafficking and prevent cell volume increment, DCs will release part of their contents back to the extracellular medium while migrating. Through electrochemical measurements, we demonstrated that exocytotic events of migratory DCs could be monitored by our ITO microdevice. In addition, the transparency of ITO electrode should facilitate future combining assays of exocytosis with other fluorescence-based measurements of cell physiology. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim}, keywords = {}, pubstate = {published}, tppubtype = {article} } The design, fabrication and test of an indium tin oxide (ITO) microdevice to investigate exocytotic behaviors of migratory dendritic cells (DCs) in confined three-dimensional environment were reported in this work. Indeed, immature DCs were able to migrate into micro-fabricated biocompatible polydimethylsiloxane (PDMS) channels that mimic their natural constrained environment of tissues for patrolling in search of danger associated antigens through an endocytotic process called macropinocytosis. In order to coordinate membrane trafficking and prevent cell volume increment, DCs will release part of their contents back to the extracellular medium while migrating. Through electrochemical measurements, we demonstrated that exocytotic events of migratory DCs could be monitored by our ITO microdevice. In addition, the transparency of ITO electrode should facilitate future combining assays of exocytosis with other fluorescence-based measurements of cell physiology. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim |
Intramolecular Photogeneration of a Tyrosine Radical in a Designed Protein Article de journal Alison G Tebo; Annamaria Quaranta; Christian Herrero; Vincent L Pecoraro; Ally Aukauloo ChemPhotoChem, 1 (3), p. 89-92, 2017. @article{Tebo:2017, title = {Intramolecular Photogeneration of a Tyrosine Radical in a Designed Protein}, author = {Alison G Tebo and Annamaria Quaranta and Christian Herrero and Vincent L Pecoraro and Ally Aukauloo}, doi = {10.1002/cptc.201600044}, year = {2017}, date = {2017-02-01}, journal = {ChemPhotoChem}, volume = {1}, number = {3}, pages = {89-92}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Loss of Fourth Electron-Transferring Tryptophan in Animal (6-4) Photolyase Impairs DNA Repair Activity in Bacterial Cells Article de journal J Yamamoto; K Shimizu; T Kanda; Y Hosokawa; S Iwai; P Plaza; P Müller Biochemistry, 56 (40), p. 5356–5364, 2017. @article{Yamamoto:2017, title = {Loss of Fourth Electron-Transferring Tryptophan in Animal (6-4) Photolyase Impairs DNA Repair Activity in Bacterial Cells}, author = {J Yamamoto and K Shimizu and T Kanda and Y Hosokawa and S Iwai and P Plaza and P M\"{u}ller}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85031007482&doi=10.1021%2facs.biochem.7b00366&partnerID=40&md5=ec7f70f4b2e5c57ef35f65a223a0f0ed}, doi = {10.1021/acs.biochem.7b00366}, year = {2017}, date = {2017-01-01}, journal = {Biochemistry}, volume = {56}, number = {40}, pages = {5356--5364}, abstract = {(6-4) photolyases [(6-4)PLs] are flavoproteins that use blue light to repair the ultraviolet-induced pyrimidine(6-4)pyrimidone photoproduct in DNA. Their flavin adenine dinucleotide (FAD) cofactor can be reduced to its repair-active FADH- form by a photoinduced electron transfer reaction. In animal (6-4)PLs, a chain of four Trp residues was suggested to be involved in a stepwise transfer of an oxidation hole from the flavin to the surface of the protein. Here, we investigated the effect of mutation of the fourth Trp on the DNA photorepair activity of Xenopus laevis (6-4)PL (Xl64) in bacterial cells. The photoreduction and photorepair properties of this mutant protein were independently characterized in vitro. Our results demonstrate that the mutation of the fourth Trp in Xl64 drastically impairs the DNA repair activity in cells and that this effect is due to the inhibition of the photoreduction process. We thereby show that the photoreductive formation of FADH- through the Trp tetrad is essential for the biological function of the animal (6-4)PL. The role of the Trp cascade, and of the fourth Trp in particular, is discussed. © 2017 American Chemical Society.}, keywords = {}, pubstate = {published}, tppubtype = {article} } (6-4) photolyases [(6-4)PLs] are flavoproteins that use blue light to repair the ultraviolet-induced pyrimidine(6-4)pyrimidone photoproduct in DNA. Their flavin adenine dinucleotide (FAD) cofactor can be reduced to its repair-active FADH- form by a photoinduced electron transfer reaction. In animal (6-4)PLs, a chain of four Trp residues was suggested to be involved in a stepwise transfer of an oxidation hole from the flavin to the surface of the protein. Here, we investigated the effect of mutation of the fourth Trp on the DNA photorepair activity of Xenopus laevis (6-4)PL (Xl64) in bacterial cells. The photoreduction and photorepair properties of this mutant protein were independently characterized in vitro. Our results demonstrate that the mutation of the fourth Trp in Xl64 drastically impairs the DNA repair activity in cells and that this effect is due to the inhibition of the photoreduction process. We thereby show that the photoreductive formation of FADH- through the Trp tetrad is essential for the biological function of the animal (6-4)PL. The role of the Trp cascade, and of the fourth Trp in particular, is discussed. © 2017 American Chemical Society. |
Molecular electrochemistry: A central method to understand the metabolic activation of therapeutic agents. The example of metallocifen anti-cancer drug candidates Article de journal C Amatore; E Labbé; O Buriez Current Opinion in Electrochemistry, 2 (1), p. 7–12, 2017. @article{Amatore:2017, title = {Molecular electrochemistry: A central method to understand the metabolic activation of therapeutic agents. The example of metallocifen anti-cancer drug candidates}, author = {C Amatore and E Labb\'{e} and O Buriez}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85045563679&doi=10.1016%2fj.coelec.2016.12.003&partnerID=40&md5=b531e3c0fb011e978b14c2e30a432353}, doi = {10.1016/j.coelec.2016.12.003}, year = {2017}, date = {2017-01-01}, journal = {Current Opinion in Electrochemistry}, volume = {2}, number = {1}, pages = {7--12}, abstract = {Organometallic chemistry has allowed the design and emergence of a new class of metal-based bioactive molecules and a variety of drugs used or still tested against cancer. In this field, beyond the well-known cisplatin, major contributions have focused on the functionalization of clinically validated purely organic drugs with complexes derivatized by a metallocene group. This approach, which exalts the anti-cancer properties of the organic moiety, has been successfully used to design ferrocifens. These complexes involve a ferrocenyl group covalently grafted onto the tamoxifen skeleton, the current gold standard for endocrine breast cancer therapy. Based on the same strategy, several original ruthenocene (Rc)-tamoxifen derivatives and osmocene (Os)-tamoxifen derivatives have been recently synthesized and successfully tested against breast and other cancer cells. The development of such potent metallodrugs was made possible thanks to the accurate mechanistic understanding of the metallodrugs activation provided by the synergistic combination of electrochemical and biological approaches. © 2017 Elsevier B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Organometallic chemistry has allowed the design and emergence of a new class of metal-based bioactive molecules and a variety of drugs used or still tested against cancer. In this field, beyond the well-known cisplatin, major contributions have focused on the functionalization of clinically validated purely organic drugs with complexes derivatized by a metallocene group. This approach, which exalts the anti-cancer properties of the organic moiety, has been successfully used to design ferrocifens. These complexes involve a ferrocenyl group covalently grafted onto the tamoxifen skeleton, the current gold standard for endocrine breast cancer therapy. Based on the same strategy, several original ruthenocene (Rc)-tamoxifen derivatives and osmocene (Os)-tamoxifen derivatives have been recently synthesized and successfully tested against breast and other cancer cells. The development of such potent metallodrugs was made possible thanks to the accurate mechanistic understanding of the metallodrugs activation provided by the synergistic combination of electrochemical and biological approaches. © 2017 Elsevier B.V. |
Optical control of tumor induction in the Zebrafish Article de journal Z Feng; S Nam; F Hamouri; I Aujard; B Ducos; S Vriz; M Volovitch; L Jullien; S Lin; S Weiss; D Bensimon Scientific Reports, 7 (1), 2017. @article{Feng:2017, title = {Optical control of tumor induction in the Zebrafish}, author = {Z Feng and S Nam and F Hamouri and I Aujard and B Ducos and S Vriz and M Volovitch and L Jullien and S Lin and S Weiss and D Bensimon}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028028076&doi=10.1038%2fs41598-017-09697-x&partnerID=40&md5=0590b4f2bc458a31e987356701b6c9f7}, doi = {10.1038/s41598-017-09697-x}, year = {2017}, date = {2017-01-01}, journal = {Scientific Reports}, volume = {7}, number = {1}, abstract = {The zebrafish has become an increasingly popular and valuable cancer model over the past few decades. While most zebrafish cancer models are generated by expressing mammalian oncogenes under tissue-specific promoters, here we describe a method that allows for the precise optical control of oncogene expression in live zebrafish. We utilize this technique to transiently or constitutively activate a typical human oncogene, kRASG12V, in zebrafish embryos and investigate the developmental and tumorigenic phenotypes. We demonstrate the spatiotemporal control of oncogene expression in live zebrafish, and characterize the different tumorigenic probabilities when kRASG12V is expressed transiently or constitutively at different developmental stages. Moreover, we show that light can be used to activate oncogene expression in selected tissues and single cells without tissue-specific promoters. Our work presents a novel approach to initiate and study cancer in zebrafish, and the high spatiotemporal resolution of this method makes it a valuable tool for studying cancer initiation from single cells. © 2017 The Author(s).}, keywords = {}, pubstate = {published}, tppubtype = {article} } The zebrafish has become an increasingly popular and valuable cancer model over the past few decades. While most zebrafish cancer models are generated by expressing mammalian oncogenes under tissue-specific promoters, here we describe a method that allows for the precise optical control of oncogene expression in live zebrafish. We utilize this technique to transiently or constitutively activate a typical human oncogene, kRASG12V, in zebrafish embryos and investigate the developmental and tumorigenic phenotypes. We demonstrate the spatiotemporal control of oncogene expression in live zebrafish, and characterize the different tumorigenic probabilities when kRASG12V is expressed transiently or constitutively at different developmental stages. Moreover, we show that light can be used to activate oncogene expression in selected tissues and single cells without tissue-specific promoters. Our work presents a novel approach to initiate and study cancer in zebrafish, and the high spatiotemporal resolution of this method makes it a valuable tool for studying cancer initiation from single cells. © 2017 The Author(s). |