You will find below the publication list of our pole.
For the publication list of each pole member, please see his/her personal webpage.
2019 |
Fatima Hamouri; Weiting Zhang; Isabelle Aujard; Thomas Le Saux; Bertrand Ducos; Sophie Vriz; Ludovic Jullien; David Bensimon Academic Press, 2019. @book{hamouri_chapter_2019, title = {Chapter One - Optical control of protein activity and gene expression by photoactivation of caged cyclofen}, author = {Fatima Hamouri and Weiting Zhang and Isabelle Aujard and Thomas Le Saux and Bertrand Ducos and Sophie Vriz and Ludovic Jullien and David Bensimon}, editor = {Alexander Deiters}, url = {https://www.sciencedirect.com/science/article/pii/S0076687919301247}, doi = {10.1016/bs.mie.2019.04.009}, year = {2019}, date = {2019-01-01}, urldate = {2023-10-31}, booktitle = {Methods in Enzymology}, volume = {624}, pages = {1--23}, publisher = {Academic Press}, series = {Optochemical Biology}, abstract = {The use of light to control the expression of genes and the activity of proteins is a rapidly expanding field. While many of these approaches use a fusion between a light activatable protein and the protein of interest to control the activity of the latter, it is also possible to control the activity of a protein by uncaging a specific ligand. In that context, controlling the activation of a protein fused to the modified estrogen receptor (ERT) by uncaging its ligand cyclofen-OH has emerged as a generic and versatile method to control the activation of proteins quantitatively, quickly and locally in a live organism. Here, we present the experimental details behind this approach.}, keywords = {}, pubstate = {published}, tppubtype = {book} } The use of light to control the expression of genes and the activity of proteins is a rapidly expanding field. While many of these approaches use a fusion between a light activatable protein and the protein of interest to control the activity of the latter, it is also possible to control the activity of a protein by uncaging a specific ligand. In that context, controlling the activation of a protein fused to the modified estrogen receptor (ERT) by uncaging its ligand cyclofen-OH has emerged as a generic and versatile method to control the activation of proteins quantitatively, quickly and locally in a live organism. Here, we present the experimental details behind this approach. |
Cover Feature: Fundamental Input of Analytical Electrochemistry in the Determination of Intermediates and Reaction Mechanisms in Electrosynthetic Processes Article de journal E Labbe; O Buriez Chemelectrochem, 6 (16), p. 4061, 2019. @article{, title = {Cover Feature: Fundamental Input of Analytical Electrochemistry in the Determination of Intermediates and Reaction Mechanisms in Electrosynthetic Processes}, author = {E Labbe and O Buriez}, url = {https://doi.org/10.1002/celc.201901158}, doi = {10.1002/celc.201901158}, year = {2019}, date = {2019-01-01}, journal = {Chemelectrochem}, volume = {6}, number = {16}, pages = {4061}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Distinctive Low-Resolution Structural Features of Dimers of Antibody–Drug Conjugates and Parent Antibody Determined by Small-Angle X-ray Scattering Article de journal Didier Law-Hine; Sergii Rudiuk; Audrey Bonestebe; Romain Ienco; Sylvain Huille; Christophe Tribet Mol. Pharmaceutics, 16 (12), p. 4902-4912, 2019. @article{Law-Hine2019, title = {Distinctive Low-Resolution Structural Features of Dimers of Antibody\textendashDrug Conjugates and Parent Antibody Determined by Small-Angle X-ray Scattering}, author = {Didier Law-Hine and Sergii Rudiuk and Audrey Bonestebe and Romain Ienco and Sylvain Huille and Christophe Tribet}, doi = {10.1021/acs.molpharmaceut.9b00792}, year = {2019}, date = {2019-10-16}, journal = {Mol. Pharmaceutics}, volume = {16}, number = {12}, pages = {4902-4912}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Diverting photosynthetic electrons from suspensions of Chlamydomonas reinhardtii algae - New insights using an electrochemical well device Article de journal A Sayegh; G Longatte; O Buriez; F -A Wollman; M Guille-Collignon; E Labbé; J Delacotte; F Lemaître Electrochimica Acta, 304 , p. 465 - 473, 2019, ISSN: 0013-4686. @article{SAYEGH2019465, title = {Diverting photosynthetic electrons from suspensions of Chlamydomonas reinhardtii algae - New insights using an electrochemical well device}, author = {A Sayegh and G Longatte and O Buriez and F -A Wollman and M Guille-Collignon and E Labb\'{e} and J Delacotte and F Lema\^{i}tre}, url = {http://www.sciencedirect.com/science/article/pii/S0013468619303718}, doi = {https://doi.org/10.1016/j.electacta.2019.02.105}, issn = {0013-4686}, year = {2019}, date = {2019-01-01}, journal = {Electrochimica Acta}, volume = {304}, pages = {465 - 473}, abstract = {In the last years, many strategies have been developed to benefit from oxygenic photosynthesis in the present context of renewable energies. To achieve this, bioelectricity may be produced by using photosynthetic components involved in anodic or cathodic compartments. In this respect, harvesting photosynthetic electrons from living biological systems appears to be an encouraging approach. However it raises the question of the most suitable electrochemical device. In this work, we describe and analyze the performances of an electrochemical device based on a millimeter sized well involving a gold surface as a working electrode. Photocurrents were generated by suspensions of Chlamydomonas reinhardtii algae using quinones as mediators under different experimental conditions. Chronoamperometry and cyclic voltammetry measurements gave insight into the use of this device to investigate important issues (harvesting and poisoning by quinones, photoinactivation…). Furthermore, by introducing a kinetic model originally developed for homogeneous catalytic systems, the kinetics of the electron diverting from this system (Chlamydomonas reinhardtii algae + 2,6-DCBQ + miniaturized setup) can be estimated. All these results demonstrate that this experimental configuration is suitable for future works devoted to the choice of the best parameters in terms of long lasting performances.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In the last years, many strategies have been developed to benefit from oxygenic photosynthesis in the present context of renewable energies. To achieve this, bioelectricity may be produced by using photosynthetic components involved in anodic or cathodic compartments. In this respect, harvesting photosynthetic electrons from living biological systems appears to be an encouraging approach. However it raises the question of the most suitable electrochemical device. In this work, we describe and analyze the performances of an electrochemical device based on a millimeter sized well involving a gold surface as a working electrode. Photocurrents were generated by suspensions of Chlamydomonas reinhardtii algae using quinones as mediators under different experimental conditions. Chronoamperometry and cyclic voltammetry measurements gave insight into the use of this device to investigate important issues (harvesting and poisoning by quinones, photoinactivation…). Furthermore, by introducing a kinetic model originally developed for homogeneous catalytic systems, the kinetics of the electron diverting from this system (Chlamydomonas reinhardtii algae + 2,6-DCBQ + miniaturized setup) can be estimated. All these results demonstrate that this experimental configuration is suitable for future works devoted to the choice of the best parameters in terms of long lasting performances. |
Electroactive fluorescent false neurotransmitter FFN102 partially replaces dopamine in PC12 cell vesicles Article de journal L Hu; A Savy; L Grimaud; M Guille-Collignon; F Lemaître; C Amatore; J Delacotte Biophysical Chemistry, 245 , p. 1–5, 2019. @article{Hu:2019, title = {Electroactive fluorescent false neurotransmitter FFN102 partially replaces dopamine in PC12 cell vesicles}, author = {L Hu and A Savy and L Grimaud and M Guille-Collignon and F Lema\^{i}tre and C Amatore and J Delacotte}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85057201704&doi=10.1016%2fj.bpc.2018.11.001&partnerID=40&md5=28655b4c152ce0fc51fc037feefcff97}, doi = {10.1016/j.bpc.2018.11.001}, year = {2019}, date = {2019-01-01}, journal = {Biophysical Chemistry}, volume = {245}, pages = {1--5}, abstract = {In the last decade, following fluorescent dyes and protein tags, pH sensitive false fluorescent neurotransmitters (FFN) were introduced and were valuable for labeling secretory vesicles and monitoring exocytosis at living cells. In particular, the synthetic analog of neurotransmitters FFN102 was shown to be an electroactive probe. Here, we show that FFN102 is suitable to be used as a bioanalytic probe at the widely used PC12 cell model. FFN102 was uptaken in the secretory vesicles of PC12 cells, partially replacing the endogenous dopamine stored in these vesicles. The different oxidation potentials of dopamine and FFN102 allowed to determine that ca. 12% of dopamine was replaced by FFN102. Moreover, the FFN102 was found to be over released through the initial fusion pore suggesting that it was mostly uptaken in fast diffusion compartment of the vesicles. © 2018 Elsevier B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In the last decade, following fluorescent dyes and protein tags, pH sensitive false fluorescent neurotransmitters (FFN) were introduced and were valuable for labeling secretory vesicles and monitoring exocytosis at living cells. In particular, the synthetic analog of neurotransmitters FFN102 was shown to be an electroactive probe. Here, we show that FFN102 is suitable to be used as a bioanalytic probe at the widely used PC12 cell model. FFN102 was uptaken in the secretory vesicles of PC12 cells, partially replacing the endogenous dopamine stored in these vesicles. The different oxidation potentials of dopamine and FFN102 allowed to determine that ca. 12% of dopamine was replaced by FFN102. Moreover, the FFN102 was found to be over released through the initial fusion pore suggesting that it was mostly uptaken in fast diffusion compartment of the vesicles. © 2018 Elsevier B.V. |
Fundamental Input of Analytical Electrochemistry in the Determination of Intermediates and Reaction Mechanisms in Electrosynthetic Processes Article de journal E Labbé; O Buriez ChemElectroChem, 6 (16), p. 4118-4125, 2019, (cited By 1). @article{Labb\'{e}20194118, title = {Fundamental Input of Analytical Electrochemistry in the Determination of Intermediates and Reaction Mechanisms in Electrosynthetic Processes}, author = {E Labb\'{e} and O Buriez}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85063908009&doi=10.1002%2fcelc.201900045&partnerID=40&md5=be86c0958f347ea6b4d1d62a0526e44c}, doi = {10.1002/celc.201900045}, year = {2019}, date = {2019-01-01}, journal = {ChemElectroChem}, volume = {6}, number = {16}, pages = {4118-4125}, note = {cited By 1}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Live Cell Super Resolution Imaging by Radial Fluctuations Using Fluorogen Binding Tags Article de journal Muthukumaran Venkatachalapathy; Vivek Belapurkar; Mini Jose; Arnaud Gautier; Deepak Nair Nanoscale, 11 (8), p. 3626-3632, 2019, ISSN: 2040-3364. @article{RN45, title = {Live Cell Super Resolution Imaging by Radial Fluctuations Using Fluorogen Binding Tags}, author = {Muthukumaran Venkatachalapathy and Vivek Belapurkar and Mini Jose and Arnaud Gautier and Deepak Nair}, doi = {10.1039/C8NR07809B}, issn = {2040-3364}, year = {2019}, date = {2019-01-01}, journal = {Nanoscale}, volume = {11}, number = {8}, pages = {3626-3632}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Nanoparticle-based local translation reveals mRNA as a translation-coupled scaffold with anchoring function Article de journal Shunnichi Kashida; Dan Ohtan Wang; Hirohide Saito; Zoher Gueroui Proceedings of the National Academy of Sciences of the United States of America, 2019, ISSN: 10916490. @article{Kashida2019, title = {Nanoparticle-based local translation reveals mRNA as a translation-coupled scaffold with anchoring function}, author = {Shunnichi Kashida and Dan Ohtan Wang and Hirohide Saito and Zoher Gueroui}, doi = {10.1073/pnas.1900310116}, issn = {10916490}, year = {2019}, date = {2019-01-01}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, abstract = {The spatial regulation of messenger RNA (mRNA) translation is central to cellular functions and relies on numerous complex processes. Biomimetic approaches could bypass these endogenous complex processes, improve our comprehension of the regulation, and allow for controlling local translation regulations and functions. However, the causality between local translation and nascent protein function remains elusive. Here, we developed a nanoparticle (NP)-based strategy to magnetically control mRNA spatial patterns in mammalian cell extracts and investigate how local translation impacts nascent protein localization and function. By monitoring the translation of the magnetically localized mRNAs, we show that mRNA\textendashNP complexes operate as a source for the continuous production of proteins from defined positions. By applying this approach to actin-binding proteins, we triggered the local formation of actin cytoskeletons and identified the minimal requirements for spatial control of the actin filament network. In addition, our bottom-up approach identified a role for mRNA as a translation-coupled scaffold for the function of nascent N-terminal protein domains. Our approach will serve as a platform for regulating mRNA localization and investigating the function of nascent protein domains during translation.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The spatial regulation of messenger RNA (mRNA) translation is central to cellular functions and relies on numerous complex processes. Biomimetic approaches could bypass these endogenous complex processes, improve our comprehension of the regulation, and allow for controlling local translation regulations and functions. However, the causality between local translation and nascent protein function remains elusive. Here, we developed a nanoparticle (NP)-based strategy to magnetically control mRNA spatial patterns in mammalian cell extracts and investigate how local translation impacts nascent protein localization and function. By monitoring the translation of the magnetically localized mRNAs, we show that mRNA–NP complexes operate as a source for the continuous production of proteins from defined positions. By applying this approach to actin-binding proteins, we triggered the local formation of actin cytoskeletons and identified the minimal requirements for spatial control of the actin filament network. In addition, our bottom-up approach identified a role for mRNA as a translation-coupled scaffold for the function of nascent N-terminal protein domains. Our approach will serve as a platform for regulating mRNA localization and investigating the function of nascent protein domains during translation. |
New Mechanistic Insights into Osmium-Based Tamoxifen Derivatives Article de journal H Z S Lee; F Chau; S Top; G Jaouen; A Vessieres; E Labbe; O Buriez Electrochimica Acta, 302 , p. 130-136, 2019, ISSN: 0013-4686. @article{RN52, title = {New Mechanistic Insights into Osmium-Based Tamoxifen Derivatives}, author = {H Z S Lee and F Chau and S Top and G Jaouen and A Vessieres and E Labbe and O Buriez}, doi = {10.1016/j.electacta.2019.02.019}, issn = {0013-4686}, year = {2019}, date = {2019-01-01}, journal = {Electrochimica Acta}, volume = {302}, pages = {130-136}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Photosensitized oxidative addition to gold(i) enables alkynylative cyclization of o-alkylnylphenols with iodoalkynes Article de journal Zhonghua Xia; Vincent Corcé; Fen Zhao; Cédric Przybylski; Agathe Espagne; Ludovic Jullien; Thomas Le Saux; Yves Gimbert; Héloïse Dossmann; Virginie Mouriès-Mansuy; Cyril Ollivier; Louis Fensterbank Nature Chemistry, 11 (9), p. 797-805, 2019, ISSN: 1755-4349. @article{RN1_28, title = {Photosensitized oxidative addition to gold(i) enables alkynylative cyclization of o-alkylnylphenols with iodoalkynes}, author = {Zhonghua Xia and Vincent Corc\'{e} and Fen Zhao and C\'{e}dric Przybylski and Agathe Espagne and Ludovic Jullien and Thomas Le Saux and Yves Gimbert and H\'{e}lo\"{i}se Dossmann and Virginie Mouri\`{e}s-Mansuy and Cyril Ollivier and Louis Fensterbank}, url = {https://doi.org/10.1038/s41557-019-0295-9}, doi = {10.1038/s41557-019-0295-9}, issn = {1755-4349}, year = {2019}, date = {2019-01-01}, journal = {Nature Chemistry}, volume = {11}, number = {9}, pages = {797-805}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Photosensitized oxidative addition to gold(I) enables alkynylative cyclization of o-alkylnylphenols with iodoalkynes Article de journal Z H Xia; V Corce; F Zhao; C Przybylski; A Espagne; L Jullien; T Le Saux; Y Gimbert; H Dossmann; V Mouries-Mansuy; C Ollivier; L Fensterbank Nature Chemistry, 11 (9), p. 797-805, 2019, ISSN: 1755-4330. @article{RN3s, title = {Photosensitized oxidative addition to gold(I) enables alkynylative cyclization of o-alkylnylphenols with iodoalkynes}, author = {Z H Xia and V Corce and F Zhao and C Przybylski and A Espagne and L Jullien and T Le Saux and Y Gimbert and H Dossmann and V Mouries-Mansuy and C Ollivier and L Fensterbank}, url = {<Go to ISI>://WOS:000483307000010}, doi = {10.1038/s41557-019-0295-9}, issn = {1755-4330}, year = {2019}, date = {2019-01-01}, journal = {Nature Chemistry}, volume = {11}, number = {9}, pages = {797-805}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Redox-Triggered Control of Cell Adhesion and Deadhesion on Poly(lysine)-g-poly(ethylene oxide) Adlayers. Article de journal Louise Hespel; Julien Dupré de Baubigny; Pierre Lalanne; Simon de Beco; Mathieu Coppey; Catherine Villard; Vincent Humblot; Emmanuelle Marie; Christophe Tribet ACS Applied Bio Materials, 2 (10), p. 4367-4376, 2019. @article{Hespel2019, title = {Redox-Triggered Control of Cell Adhesion and Deadhesion on Poly(lysine)-g-poly(ethylene oxide) Adlayers.}, author = {Louise Hespel and Julien Dupr\'{e} de Baubigny and Pierre Lalanne and Simon de Beco and Mathieu Coppey and Catherine Villard and Vincent Humblot and Emmanuelle Marie and Christophe Tribet}, doi = {10.1021/acsabm.9b00601}, year = {2019}, date = {2019-09-10}, journal = {ACS Applied Bio Materials}, volume = {2}, number = {10}, pages = {4367-4376}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
RNA is a critical element for the sizing and the composition of phase-separated RNA–protein condensates Article de journal Marina Garcia-Jove Navarro; Shunnichi Kashida; Racha Chouaib; Sylvie Souquere; Gérard Pierron; Dominique Weil; Zoher Gueroui Nature Communications, 2019, ISSN: 2041-1723. @article{Garcia-JoveNavarro2019, title = {RNA is a critical element for the sizing and the composition of phase-separated RNA\textendashprotein condensates}, author = {Marina {Garcia-Jove Navarro} and Shunnichi Kashida and Racha Chouaib and Sylvie Souquere and G\'{e}rard Pierron and Dominique Weil and Zoher Gueroui}, doi = {10.1038/s41467-019-11241-6}, issn = {2041-1723}, year = {2019}, date = {2019-01-01}, journal = {Nature Communications}, abstract = {Liquid-liquid phase separation is thought to be a key organizing principle in eukaryotic cells to generate highly concentrated dynamic assemblies, such as the RNP granules. Numerous in vitro approaches have validated this model, yet a missing aspect is to take into consideration the complex molecular mixture and promiscuous interactions found in vivo. Here we report the versatile scaffold "ArtiG" to generate concentration-dependent RNA-protein condensates within living cells, as a bottom-up approach to study the impact of co-segregated endogenous components on phase separation. We demonstrate that intracellular RNA seeds the nucleation of the condensates, as it provides molecular cues to locally coordinate the formation of endogenous high order RNP assemblies. Interestingly, the co-segregation of intracellular components ultimately impacts the size of the phase-separated condensates. Thus, RNA arises as an architectural element that can influence the composition and the morphological outcome of the condensate phases in an intracellular context.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Liquid-liquid phase separation is thought to be a key organizing principle in eukaryotic cells to generate highly concentrated dynamic assemblies, such as the RNP granules. Numerous in vitro approaches have validated this model, yet a missing aspect is to take into consideration the complex molecular mixture and promiscuous interactions found in vivo. Here we report the versatile scaffold "ArtiG" to generate concentration-dependent RNA-protein condensates within living cells, as a bottom-up approach to study the impact of co-segregated endogenous components on phase separation. We demonstrate that intracellular RNA seeds the nucleation of the condensates, as it provides molecular cues to locally coordinate the formation of endogenous high order RNP assemblies. Interestingly, the co-segregation of intracellular components ultimately impacts the size of the phase-separated condensates. Thus, RNA arises as an architectural element that can influence the composition and the morphological outcome of the condensate phases in an intracellular context. |
Self-aggregation of oxidized procyanidins contributes to the formation of heat-reversible haze in apple-based liqueur wine Article de journal M Millet; P Poupard; S Guilois-Dubois; D Zanchi; S Guyot Food Chemistry, 276 , p. 797–805, 2019. @article{Millet:2019, title = {Self-aggregation of oxidized procyanidins contributes to the formation of heat-reversible haze in apple-based liqueur wine}, author = {M Millet and P Poupard and S Guilois-Dubois and D Zanchi and S Guyot}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85055330959&doi=10.1016%2fj.foodchem.2018.09.171&partnerID=40&md5=023402dcebfde4b3a1f3f506c5b74529}, doi = {10.1016/j.foodchem.2018.09.171}, year = {2019}, date = {2019-01-01}, journal = {Food Chemistry}, volume = {276}, pages = {797--805}, abstract = {The ability of tannins to self-associate or form complexes with other macromolecules has important nutritional implications but can also result in defects in beverages. In addition, oxidation may be involved in the aggregation properties of tannins. In order to assess the impact of tannin oxidation on their self-association, oligomeric procyanidins were oxidized in a model solution and their aggregation kinetics were studied using light scattering. Under the conditions tested, only oxidized procyanidins were involved in haze formation. An increase in the level of oxidation and the degree of polymerization of procyanidins enhanced aggregation. Procyanidin oxidation products were depolymerized and the evolution of their markers was monitored throughout the aggregation process using liquid chromatography coupled with mass spectrometry. This revealed the involvement of intramolecular coupling in reversible haze formation. The haze formed in a model solution was partially reversible at high temperature. This property was similar in pommeau, an apple-based beverage. This work highlighted the involvement of oxidized tannins in reversible haze. © 2018 Elsevier Ltd}, keywords = {}, pubstate = {published}, tppubtype = {article} } The ability of tannins to self-associate or form complexes with other macromolecules has important nutritional implications but can also result in defects in beverages. In addition, oxidation may be involved in the aggregation properties of tannins. In order to assess the impact of tannin oxidation on their self-association, oligomeric procyanidins were oxidized in a model solution and their aggregation kinetics were studied using light scattering. Under the conditions tested, only oxidized procyanidins were involved in haze formation. An increase in the level of oxidation and the degree of polymerization of procyanidins enhanced aggregation. Procyanidin oxidation products were depolymerized and the evolution of their markers was monitored throughout the aggregation process using liquid chromatography coupled with mass spectrometry. This revealed the involvement of intramolecular coupling in reversible haze formation. The haze formed in a model solution was partially reversible at high temperature. This property was similar in pommeau, an apple-based beverage. This work highlighted the involvement of oxidized tannins in reversible haze. © 2018 Elsevier Ltd |
Single-Molecule Localization Microscopy with the Fluorescence-Activating and Absorption-Shifting Tag (FAST) System Article de journal Elizabeth M Smith; Arnaud Gautier; Elias M Puchner ACS Chemical Biology, 14 (6), p. 1115-1120, 2019, ISSN: 1554-8929. @article{Smith2019, title = {Single-Molecule Localization Microscopy with the Fluorescence-Activating and Absorption-Shifting Tag (FAST) System}, author = {Elizabeth M Smith and Arnaud Gautier and Elias M Puchner}, url = {https://doi.org/10.1021/acschembio.9b00149}, doi = {10.1021/acschembio.9b00149}, issn = {1554-8929}, year = {2019}, date = {2019-06-21}, journal = {ACS Chemical Biology}, volume = {14}, number = {6}, pages = {1115-1120}, publisher = {American Chemical Society}, abstract = {We develop and employ the Fluorescence-Activating and absorption-Shifting Tag (FAST) system for super-resolution (SR) imaging and single-molecule tracking based on single-molecule localizations. The fast off rate of fluorogen binding, combined with its spatially well-separated labeling of the densely expressed FAST fusion proteins, allowed single-molecule measurements to be performed in both living and fixed cells. The well-separated fluorescence localization density was achieved by either reversibly controlling the fluorogen concentration or by irreversibly photobleaching the FAST-fluorogen complex. The experimentally determined resolution of 28 nm allowed us to resolve Ensconsin-labeled microtubules and to track single molecules in mitochondria. Our results demonstrate that FAST is well-suited for single-molecule localization microscopy (SMLM). The small size and the availability of spectrally distinct fluorogens present unique advantages of the FAST system as a potential orthogonal labeling strategy that could be applied in conjunction with existing super-resolution dyes and photoactivatable proteins in versatile imaging applications.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We develop and employ the Fluorescence-Activating and absorption-Shifting Tag (FAST) system for super-resolution (SR) imaging and single-molecule tracking based on single-molecule localizations. The fast off rate of fluorogen binding, combined with its spatially well-separated labeling of the densely expressed FAST fusion proteins, allowed single-molecule measurements to be performed in both living and fixed cells. The well-separated fluorescence localization density was achieved by either reversibly controlling the fluorogen concentration or by irreversibly photobleaching the FAST-fluorogen complex. The experimentally determined resolution of 28 nm allowed us to resolve Ensconsin-labeled microtubules and to track single molecules in mitochondria. Our results demonstrate that FAST is well-suited for single-molecule localization microscopy (SMLM). The small size and the availability of spectrally distinct fluorogens present unique advantages of the FAST system as a potential orthogonal labeling strategy that could be applied in conjunction with existing super-resolution dyes and photoactivatable proteins in versatile imaging applications. |
Tannin-controlled micelles and fibrils of kappa-casein Article de journal W Ma; C Tribet; S Guyot; D Zanchi Journal of Chemical Physics, 151 (24), 2019, ISSN: 0021-9606. @article{RN264, title = {Tannin-controlled micelles and fibrils of kappa-casein}, author = {W Ma and C Tribet and S Guyot and D Zanchi}, url = {<Go to ISI>://WOS:000513160200063}, doi = {10.1063/1.5128057}, issn = {0021-9606}, year = {2019}, date = {2019-01-01}, journal = {Journal of Chemical Physics}, volume = {151}, number = {24}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
The Glowing Panoply of Fluorogen-based Markers for Advanced Bioimaging Book Chapter A Gautier The Glowing Panoply of Fluorogen-based Markers for Advanced Bioimaging, Chapitre 3, 2019. @inbook{Gautier:2019, title = {The Glowing Panoply of Fluorogen-based Markers for Advanced Bioimaging}, author = {A Gautier}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85056600039&doi=10.1039%2f9781788013284-00041&partnerID=40&md5=5d754dc76f6a9652835200ef4214a41a}, doi = {10.1039/9781788013284-00041}, year = {2019}, date = {2019-01-01}, booktitle = {The Glowing Panoply of Fluorogen-based Markers for Advanced Bioimaging}, chapter = {3}, series = {Comprehensive Series in Photochemical and Photobiological Sciences}, abstract = {The biological sciences nowadays rely extensively on imaging tools to decipher the complexity of living organisms. Fluorescence microscopy allows the study of biological processes with unprecedented temporal and spatial resolution. The revolution in fluorescence imaging has been the development of a large toolbox of fluorescent proteins able to reveal the abundance, position and dynamics of proteins. The fluorescence toolbox has recently been expanded with innovative reporters that permit proteins, and other biomolecules such as RNA, to be visualized in new ways. These innovative reporters are bipartite systems composed of a genetically encoded tag forming a fluorescent complex with a small organic fluorogenic chromophore (also called fluorogens). This chapter is a user-oriented presentation of some of the most mature fluorogen-based markers available to biologists. © 2019 European Society for Photobiology.}, keywords = {}, pubstate = {published}, tppubtype = {inbook} } The biological sciences nowadays rely extensively on imaging tools to decipher the complexity of living organisms. Fluorescence microscopy allows the study of biological processes with unprecedented temporal and spatial resolution. The revolution in fluorescence imaging has been the development of a large toolbox of fluorescent proteins able to reveal the abundance, position and dynamics of proteins. The fluorescence toolbox has recently been expanded with innovative reporters that permit proteins, and other biomolecules such as RNA, to be visualized in new ways. These innovative reporters are bipartite systems composed of a genetically encoded tag forming a fluorescent complex with a small organic fluorogenic chromophore (also called fluorogens). This chapter is a user-oriented presentation of some of the most mature fluorogen-based markers available to biologists. © 2019 European Society for Photobiology. |
Tunable and switchable soft adsorption of polymer-coated microparticles on a flat substrate Article de journal Giuseppe Boniello; Christophe Tribet; Emmanuelle Marie; Vincent Croquette; Dražen Zanchi Colloids and Surfaces A: Physicochemical and Engineering Aspects, 575 , p. 199-204, 2019, ISSN: 0927-7757. @article{Boniello2019, title = {Tunable and switchable soft adsorption of polymer-coated microparticles on a flat substrate}, author = {Giuseppe Boniello and Christophe Tribet and Emmanuelle Marie and Vincent Croquette and Dra\v{z}en Zanchi}, editor = {Elsevier}, url = {http://www.sciencedirect.com/science/article/pii/S0927775719301797}, doi = {10.1016/j.colsurfa.2019.04.081}, issn = {0927-7757}, year = {2019}, date = {2019-08-20}, journal = {Colloids and Surfaces A: Physicochemical and Engineering Aspects}, volume = {575}, pages = {199-204}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ultrafast Oxidation of a Tyrosine by Proton-Coupled Electron Transfer Promotes Light Activation of an Animal-like Cryptochrome Article de journal Fabien Lacombat; Agathe Espagne; Nadia Dozova; Pascal Plaza; Pavel Müller; Klaus Brettel; Sophie Franz-Badur; Lars-Oliver Essen Journal of the American Chemical Society, 141 (34), p. 13394-13409, 2019, ISSN: 0002-7863. @article{RN479, title = {Ultrafast Oxidation of a Tyrosine by Proton-Coupled Electron Transfer Promotes Light Activation of an Animal-like Cryptochrome}, author = {Fabien Lacombat and Agathe Espagne and Nadia Dozova and Pascal Plaza and Pavel M\"{u}ller and Klaus Brettel and Sophie Franz-Badur and Lars-Oliver Essen}, url = {https://doi.org/10.1021/jacs.9b03680}, doi = {10.1021/jacs.9b03680}, issn = {0002-7863}, year = {2019}, date = {2019-01-01}, journal = {Journal of the American Chemical Society}, volume = {141}, number = {34}, pages = {13394-13409}, abstract = {The animal-like cryptochrome of Chlamydomonas reinhardtii (CraCRY) is a recently discovered photoreceptor that controls the transcriptional profile and sexual life cycle of this alga by both blue and red light. CraCRY has the uncommon feature of efficient formation and longevity of the semireduced neutral form of its FAD cofactor upon blue light illumination. Tyrosine Y373 plays a crucial role by elongating , as fourth member, the electron transfer (ET) chain found in most other cryptochromes and DNA photolyases, which comprises a conserved tryptophan triad. Here, we report the full mechanism of light-induced FADH• formation in CraCRY using transient absorption spectroscopy from hundreds of femtoseconds to seconds. Electron transfer starts from ultrafast reduction of excited FAD to FAD•\textendash by the proximal tryptophan (0.4 ps) and is followed by delocalized migration of the produced WH•+ radical along the tryptophan triad (∼4 and ∼50 ps). Oxidation of Y373 by coupled ET to WH•+ and deprotonation then proceeds in ∼800 ps, without any significant kinetic isotope effect, nor a pH effect between pH 6.5 and 9.0. The FAD•\textendash/Y373• pair is formed with high quantum yield (∼60%); its intrinsic decay by recombination is slow (∼50 ms), favoring reduction of Y373• by extrinsic agents and protonation of FAD•\textendash to form the long-lived, red-light absorbing FADH• species. Possible mechanisms of tyrosine oxidation by ultrafast proton-coupled ET in CraCRY, a process about 40 times faster than the archetypal tyrosine-Z oxidation in photosystem II, are discussed in detail.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The animal-like cryptochrome of Chlamydomonas reinhardtii (CraCRY) is a recently discovered photoreceptor that controls the transcriptional profile and sexual life cycle of this alga by both blue and red light. CraCRY has the uncommon feature of efficient formation and longevity of the semireduced neutral form of its FAD cofactor upon blue light illumination. Tyrosine Y373 plays a crucial role by elongating , as fourth member, the electron transfer (ET) chain found in most other cryptochromes and DNA photolyases, which comprises a conserved tryptophan triad. Here, we report the full mechanism of light-induced FADH• formation in CraCRY using transient absorption spectroscopy from hundreds of femtoseconds to seconds. Electron transfer starts from ultrafast reduction of excited FAD to FAD•– by the proximal tryptophan (0.4 ps) and is followed by delocalized migration of the produced WH•+ radical along the tryptophan triad (∼4 and ∼50 ps). Oxidation of Y373 by coupled ET to WH•+ and deprotonation then proceeds in ∼800 ps, without any significant kinetic isotope effect, nor a pH effect between pH 6.5 and 9.0. The FAD•–/Y373• pair is formed with high quantum yield (∼60%); its intrinsic decay by recombination is slow (∼50 ms), favoring reduction of Y373• by extrinsic agents and protonation of FAD•– to form the long-lived, red-light absorbing FADH• species. Possible mechanisms of tyrosine oxidation by ultrafast proton-coupled ET in CraCRY, a process about 40 times faster than the archetypal tyrosine-Z oxidation in photosystem II, are discussed in detail. |
Ultrafast photoinduced flavin dynamics in the unusual active site of the tRNA methyltransferase TrmFO Article de journal N Dozova; F Lacombat; C Bou-Nader; D Hamdane; P Plaza Physical Chemistry Chemical Physics, 21 (17), p. 8743-8756, 2019, ISSN: 1463-9076. @article{RN115, title = {Ultrafast photoinduced flavin dynamics in the unusual active site of the tRNA methyltransferase TrmFO}, author = {N Dozova and F Lacombat and C Bou-Nader and D Hamdane and P Plaza}, doi = {10.1039/c8cp06072j}, issn = {1463-9076}, year = {2019}, date = {2019-01-01}, journal = {Physical Chemistry Chemical Physics}, volume = {21}, number = {17}, pages = {8743-8756}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2018 |
A chemically encoded timer for dual molecular delivery at tailored ranges and concentrations Article de journal S Serra; A Alouane; T Le Saux; S Huvelle; R Plasson; F Schmidt; L Jullien; R Labruère Chemical Communications, 54 (49), p. 6396–6399, 2018. @article{Serra:2018, title = {A chemically encoded timer for dual molecular delivery at tailored ranges and concentrations}, author = {S Serra and A Alouane and T Le Saux and S Huvelle and R Plasson and F Schmidt and L Jullien and R Labru\`{e}re}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85048683206&doi=10.1039%2fc8cc03253j&partnerID=40&md5=6b7d3eaf2fac02d48f0cfa34e3b730b9}, doi = {10.1039/c8cc03253j}, year = {2018}, date = {2018-01-01}, journal = {Chemical Communications}, volume = {54}, number = {49}, pages = {6396--6399}, abstract = {Spatiotemporal control of molecular distribution is much in demand in many fields of chemistry. To address this goal, we exploit a low molecular weight branched self-immolative architecture, which acts as a triggerable chemically encoded timer for autonomous sequential release of two chemicals. Using a light-activated model liberating two distinct fluorophores, we generated a tunable spatially contrasted molecular distribution. © 2018 The Royal Society of Chemistry.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Spatiotemporal control of molecular distribution is much in demand in many fields of chemistry. To address this goal, we exploit a low molecular weight branched self-immolative architecture, which acts as a triggerable chemically encoded timer for autonomous sequential release of two chemicals. Using a light-activated model liberating two distinct fluorophores, we generated a tunable spatially contrasted molecular distribution. © 2018 The Royal Society of Chemistry. |
A novel diarylethene-based photoswitchable chelator for reversible release and capture of Ca2+ in aqueous media Article de journal N Dozova; G Pousse; B Barnych; J -M Mallet; J Cossy; B Valeur; P Plaza Journal of Photochemistry and Photobiology A: Chemistry, 360 , p. 181–187, 2018. @article{Dozova:2018, title = {A novel diarylethene-based photoswitchable chelator for reversible release and capture of Ca2+ in aqueous media}, author = {N Dozova and G Pousse and B Barnych and J -M Mallet and J Cossy and B Valeur and P Plaza}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85046375200&doi=10.1016%2fj.jphotochem.2018.04.029&partnerID=40&md5=acfff9bc55e2d0bb41580e84374018fc}, doi = {10.1016/j.jphotochem.2018.04.029}, year = {2018}, date = {2018-01-01}, journal = {Journal of Photochemistry and Photobiology A: Chemistry}, volume = {360}, pages = {181--187}, abstract = {The synthesis and characterisation of a novel Reversibly Photoswitchable Chelator (RPC) of calcium ions, designed as a stepping stone towards producing pulses of calcium concentration in an aqueous environment, is reported. This RPC is constituted of a photochromic diarylethene core connected on one side to a BAPTA (1,2-bis(2-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid) calcium chelator and, on the other side, to an electron-withdrawing group. The operation principle consists in photoswitching on and off an intramolecular charge transfer between one nitrogen atom of the BAPTA moiety and the electron-withdrawing group, thereby modulating the chelating affinity of BAPTA for calcium ions. Solubility of the compound in a partially aqueous solvent was achieved by grafting a short PEG (polyethylene glycol) tail to the electron-withdrawing group. A reduction of the affinity for calcium ions upon photoswitching by a factor of 3\textendash4, in the hundred nM range of dissociation constant, is reported and constitutes a proof of concept of this type of RPC. © 2018 Elsevier B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The synthesis and characterisation of a novel Reversibly Photoswitchable Chelator (RPC) of calcium ions, designed as a stepping stone towards producing pulses of calcium concentration in an aqueous environment, is reported. This RPC is constituted of a photochromic diarylethene core connected on one side to a BAPTA (1,2-bis(2-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid) calcium chelator and, on the other side, to an electron-withdrawing group. The operation principle consists in photoswitching on and off an intramolecular charge transfer between one nitrogen atom of the BAPTA moiety and the electron-withdrawing group, thereby modulating the chelating affinity of BAPTA for calcium ions. Solubility of the compound in a partially aqueous solvent was achieved by grafting a short PEG (polyethylene glycol) tail to the electron-withdrawing group. A reduction of the affinity for calcium ions upon photoswitching by a factor of 3–4, in the hundred nM range of dissociation constant, is reported and constitutes a proof of concept of this type of RPC. © 2018 Elsevier B.V. |
Actin-Network Architecture Regulates Microtubule Dynamics Article de journal A Colin; P Singaravelu; M Thery; L Blanchoin; Z Gueroui Current Biology, 28 (16), p. 2647-+, 2018, ISSN: 0960-9822. @article{RN18c, title = {Actin-Network Architecture Regulates Microtubule Dynamics}, author = {A Colin and P Singaravelu and M Thery and L Blanchoin and Z Gueroui}, url = {<Go to ISI>://WOS:000442111300035}, doi = {10.1016/j.cub.2018.06.028}, issn = {0960-9822}, year = {2018}, date = {2018-01-01}, journal = {Current Biology}, volume = {28}, number = {16}, pages = {2647-+}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Circularly Permuted Fluorogenic Proteins for the Design of Modular Biosensors Article de journal A G Tebo; F M Pimenta; M Zoumpoulaki; C Kikuti; H Sirkia; M -A Plamont; A Houdusse; A Gautier ACS Chemical Biology, 13 (9), p. 2392–2397, 2018. @article{Tebo:2018, title = {Circularly Permuted Fluorogenic Proteins for the Design of Modular Biosensors}, author = {A G Tebo and F M Pimenta and M Zoumpoulaki and C Kikuti and H Sirkia and M -A Plamont and A Houdusse and A Gautier}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85052286419&doi=10.1021%2facschembio.8b00417&partnerID=40&md5=f7f8a46015d14cb7450f3d6d2b70a529}, doi = {10.1021/acschembio.8b00417}, year = {2018}, date = {2018-01-01}, journal = {ACS Chemical Biology}, volume = {13}, number = {9}, pages = {2392--2397}, abstract = {Fluorescent reporters are essential components for the design of optical biosensors that are able to image intracellular analytes in living cells. Herein, we describe the development of circularly permuted variants of Fluorescence-Activating and absorption-Shifting Tag (FAST) and demonstrate their potential as reporting module in biosensors. Circularly permutated FAST (cpFAST) variants allow one to condition the binding and activation of a fluorogenic ligand (and thus fluorescence) to analyte recognition by coupling them with analyte-binding domains. We demonstrated their use for biosensor design by generating multicolor plug-and-play fluorogenic biosensors for imaging the intracellular levels of Ca2+ in living mammalian cells in real time. © 2018 American Chemical Society.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Fluorescent reporters are essential components for the design of optical biosensors that are able to image intracellular analytes in living cells. Herein, we describe the development of circularly permuted variants of Fluorescence-Activating and absorption-Shifting Tag (FAST) and demonstrate their potential as reporting module in biosensors. Circularly permutated FAST (cpFAST) variants allow one to condition the binding and activation of a fluorogenic ligand (and thus fluorescence) to analyte recognition by coupling them with analyte-binding domains. We demonstrated their use for biosensor design by generating multicolor plug-and-play fluorogenic biosensors for imaging the intracellular levels of Ca2+ in living mammalian cells in real time. © 2018 American Chemical Society. |
Control of Protein Activity and Gene Expression by Cyclofen-OH Uncaging Article de journal W Zhang; F Hamouri; Z Feng; I Aujard; B Ducos; S Ye; S Weiss; M Volovitch; S Vriz; L Jullien; D Bensimon ChemBioChem, 19 (12), p. 1232–1238, 2018. @article{Zhang:2018, title = {Control of Protein Activity and Gene Expression by Cyclofen-OH Uncaging}, author = {W Zhang and F Hamouri and Z Feng and I Aujard and B Ducos and S Ye and S Weiss and M Volovitch and S Vriz and L Jullien and D Bensimon}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042596408&doi=10.1002%2fcbic.201700630&partnerID=40&md5=e4da60b83f338aba093989913695947b}, doi = {10.1002/cbic.201700630}, year = {2018}, date = {2018-01-01}, journal = {ChemBioChem}, volume = {19}, number = {12}, pages = {1232--1238}, abstract = {The use of light to control the expression of genes and the activity of proteins is a rapidly expanding field. Whereas many of these approaches use fusion between a light-activable protein and the protein of interest to control the activity of the latter, it is also possible to control the activity of a protein by uncaging a specific ligand. In that context, controlling the activation of a protein fused to the modified estrogen receptor (ERT) by uncaging its ligand cyclofen-OH has emerged as a generic and versatile method to control the activation of proteins quantitatively, quickly, and locally in a live organism. We present that approach and its uses in a variety of physiological contexts. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim}, keywords = {}, pubstate = {published}, tppubtype = {article} } The use of light to control the expression of genes and the activity of proteins is a rapidly expanding field. Whereas many of these approaches use fusion between a light-activable protein and the protein of interest to control the activity of the latter, it is also possible to control the activity of a protein by uncaging a specific ligand. In that context, controlling the activation of a protein fused to the modified estrogen receptor (ERT) by uncaging its ligand cyclofen-OH has emerged as a generic and versatile method to control the activation of proteins quantitatively, quickly, and locally in a live organism. We present that approach and its uses in a variety of physiological contexts. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim |
X Liu; L Hu; N Pan; L Grimaud; E Labbé; O Buriez; J Delacotte; F Lemaître; M Guille-Collignon Biophysical Chemistry, 235 , p. 48–55, 2018. @article{Liu:2018, title = {Coupling electrochemistry and TIRF-microscopy with the fluorescent false neurotransmitter FFN102 supports the fluorescence signals during single vesicle exocytosis detection}, author = {X Liu and L Hu and N Pan and L Grimaud and E Labb\'{e} and O Buriez and J Delacotte and F Lema\^{i}tre and M Guille-Collignon}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042352158&doi=10.1016%2fj.bpc.2018.02.004&partnerID=40&md5=365430d79a0d526895e755729264d88f}, doi = {10.1016/j.bpc.2018.02.004}, year = {2018}, date = {2018-01-01}, journal = {Biophysical Chemistry}, volume = {235}, pages = {48--55}, abstract = {Applications of the Fluorescent False Neurotransmitter FFN102, an analog of biogenic neurotransmitters and a suitable probe for coupled amperometry and TIRFM (total internal reflexion fluorescence microscopy) investigations of exocytotic secretion, were considered here. The electroactivity of FFN102 was shown to very likely arise from the oxidation of its phenolic group through a CE (Chemical-Electrochemical) mechanism. Evidences that the aminoethyl group of FFN102 is the key recognition element by BON N13 cells were also provided. Amperometric measurements were then performed at the single cell level with carbon fiber electrode (CFE) or Indium Tin Oxide (ITO) surfaces. It proved the disparity of kinetic and quantitative parameters of FFN102-stained cells acquired either at cell top and bottom. Moreover, coupled analyses of FFN102 loaded vesicles allowed us to classify three types of optical signals that probably arise from secretion releases thanks to their concomitant detection with an electrochemical spike. Finally, preliminary benefits from the coupling involving FFN102 were reported in terms of origins of overlapped amperometric spikes or assignment of fluorescence extinctions to real exocytotic events. © 2018 Elsevier B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Applications of the Fluorescent False Neurotransmitter FFN102, an analog of biogenic neurotransmitters and a suitable probe for coupled amperometry and TIRFM (total internal reflexion fluorescence microscopy) investigations of exocytotic secretion, were considered here. The electroactivity of FFN102 was shown to very likely arise from the oxidation of its phenolic group through a CE (Chemical-Electrochemical) mechanism. Evidences that the aminoethyl group of FFN102 is the key recognition element by BON N13 cells were also provided. Amperometric measurements were then performed at the single cell level with carbon fiber electrode (CFE) or Indium Tin Oxide (ITO) surfaces. It proved the disparity of kinetic and quantitative parameters of FFN102-stained cells acquired either at cell top and bottom. Moreover, coupled analyses of FFN102 loaded vesicles allowed us to classify three types of optical signals that probably arise from secretion releases thanks to their concomitant detection with an electrochemical spike. Finally, preliminary benefits from the coupling involving FFN102 were reported in terms of origins of overlapped amperometric spikes or assignment of fluorescence extinctions to real exocytotic events. © 2018 Elsevier B.V. |
Delocalized hole transport coupled to sub-ns tryptophanyl deprotonation promotes photoreduction of class II photolyases Article de journal F Lacombat; A Espagne; N Dozova; P Plaza; E Ignatz; S Kiontke; L O Essen Physical Chemistry Chemical Physics, 20 (39), p. 25446-25457, 2018, ISSN: 1463-9076. @article{RN114, title = {Delocalized hole transport coupled to sub-ns tryptophanyl deprotonation promotes photoreduction of class II photolyases}, author = {F Lacombat and A Espagne and N Dozova and P Plaza and E Ignatz and S Kiontke and L O Essen}, url = {<Go to ISI>://WOS:000448345400028}, doi = {10.1039/c8cp04548h}, issn = {1463-9076}, year = {2018}, date = {2018-01-01}, journal = {Physical Chemistry Chemical Physics}, volume = {20}, number = {39}, pages = {25446-25457}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Development of a Rubredoxin-Type Center Embedded in a de Dovo-Designed Three-Helix Bundle Article de journal Alison G Tebo; Tyler B J Pinter; Ricardo García-Serres; Amy L Speelman; Cédric Tard; Olivier Sénèque; Geneviève Blondin; Jean-Marc Latour; James Penner-Hahn; Nicolai Lehnert; Vincent L Pecoraro Biochemistry, 57 (16), p. 2308-2316, 2018. @article{Tebo:2018b, title = {Development of a Rubredoxin-Type Center Embedded in a de Dovo-Designed Three-Helix Bundle}, author = {Alison G Tebo and Tyler B J Pinter and Ricardo {Garc\'{i}a-Serres} and Amy L Speelman and C\'{e}dric Tard and Olivier S\'{e}n\`{e}que and Genevi\`{e}ve Blondin and Jean-Marc Latour and James {Penner-Hahn} and Nicolai Lehnert and Vincent L Pecoraro}, doi = {10.1021/acs.biochem.8b00091}, year = {2018}, date = {2018-03-01}, journal = {Biochemistry}, volume = {57}, number = {16}, pages = {2308-2316}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Downstream Simultaneous Electrochemical Detection of Primary Reactive Oxygen and Nitrogen Species Released by Cell Populations in an Integrated Microfluidic Device Article de journal Y Li; C Sella; F Lemaître; M Guille-Collignon; C Amatore; L Thouin Analytical Chemistry, 90 (15), p. 9386–9394, 2018. @article{Li:2018, title = {Downstream Simultaneous Electrochemical Detection of Primary Reactive Oxygen and Nitrogen Species Released by Cell Populations in an Integrated Microfluidic Device}, author = {Y Li and C Sella and F Lema\^{i}tre and M Guille-Collignon and C Amatore and L Thouin}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85049664645&doi=10.1021%2facs.analchem.8b02039&partnerID=40&md5=8f713011165b3b5e06d267ffccea9277}, doi = {10.1021/acs.analchem.8b02039}, year = {2018}, date = {2018-01-01}, journal = {Analytical Chemistry}, volume = {90}, number = {15}, pages = {9386--9394}, abstract = {An innovative microfluidic platform was designed to monitor electrochemically four primary reactive oxygen (ROS) and reactive nitrogen species (RNS) released by aerobic cells. Taking advantage of the space confinement and electrode performances under flow conditions, only a few experiments were sufficient to directly provide significant statistical data relative to the average behavior of cells during oxidative-stress bursts. The microfluidic platform comprised an upstream microchamber for cell culture and four parallel microchannels located downstream for separately detecting H2O2, ONOO-, NO·, and NO2 -. Amperometric measurements were performed at highly sensitive Pt-black electrodes implemented in the microchannels. RAW 264.7 macrophage secretions triggered by a calcium ionophore were used as a way to assess the performance, sensitivity, and specificity of the integrated microfluidic device. In comparison with some previous evaluations achieved from single-cell measurements, reproducible and relevant determinations validated the proof of concept of this microfluidic platform for analyzing statistically significant oxidative-stress responses of various cell types. Copyright © 2018 American Chemical Society.}, keywords = {}, pubstate = {published}, tppubtype = {article} } An innovative microfluidic platform was designed to monitor electrochemically four primary reactive oxygen (ROS) and reactive nitrogen species (RNS) released by aerobic cells. Taking advantage of the space confinement and electrode performances under flow conditions, only a few experiments were sufficient to directly provide significant statistical data relative to the average behavior of cells during oxidative-stress bursts. The microfluidic platform comprised an upstream microchamber for cell culture and four parallel microchannels located downstream for separately detecting H2O2, ONOO-, NO·, and NO2 -. Amperometric measurements were performed at highly sensitive Pt-black electrodes implemented in the microchannels. RAW 264.7 macrophage secretions triggered by a calcium ionophore were used as a way to assess the performance, sensitivity, and specificity of the integrated microfluidic device. In comparison with some previous evaluations achieved from single-cell measurements, reproducible and relevant determinations validated the proof of concept of this microfluidic platform for analyzing statistically significant oxidative-stress responses of various cell types. Copyright © 2018 American Chemical Society. |
Electrochemical switching fluorescence emission in rhodamine derivatives Article de journal M Čížková; L Cattiaux; J -M Mallet; E Labbé; O Buriez Electrochimica Acta, 260 , p. 589–597, 2018. @article{Cizkova:2018a, title = {Electrochemical switching fluorescence emission in rhodamine derivatives}, author = {M \v{C}\'{i}\v{z}kov\'{a} and L Cattiaux and J -M Mallet and E Labb\'{e} and O Buriez}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85038870042&doi=10.1016%2fj.electacta.2017.12.104&partnerID=40&md5=6a1537d7de8ad37549ef796b0c6f2642}, doi = {10.1016/j.electacta.2017.12.104}, year = {2018}, date = {2018-01-01}, journal = {Electrochimica Acta}, volume = {260}, pages = {589--597}, abstract = {Three rhodamine derivatives exhibiting electrofluorochromic properties were investigated by cyclic voltammetry and UV\textendashVis/fluorescence spectroelectrochemistry. Rhodamine 101 (Rh101, compound 1) was used as a reference model. In compound 2, the carboxylate anion of Rh101 was replaced by an alkyne moiety to allow further functionalization. The compound 3 was prepared from 2 by conversion of the alkyne to a triazole group bearing an alkyl chain with an alcohol function. These three rhodamine derivatives exhibited similar electrochemical behaviors. Their mono-electronic reductions produced the corresponding radical species which were stable on the time-scale of cyclic voltammetry. Additional reduction of electrogenerated radicals produced unstable anions which underwent subsequent chemical reaction, most likely protonation. Based on cyclic voltammetry investigations, absorption and fluorescence spectroelectrochemistry were then performed on compounds 1, 2, 3 and their parent reduced radicals 1a, 2a, 3a. UV\textendashVis spectroelectrochemistry, combined with TD-DFT calculation, confirmed the formation of radicals upon mono-electronic reduction of starting rhodamines. Fluorescence spectroelectrochemistry showed that, contrary to their parent molecules, electrogenerated radicals were non-fluorescent. Electrochemical fluorescence extinction was successfully achieved with all studied compounds. Moreover, compound 1 underwent on/off switching between fluorescent and non-fluorescent states repeatedly. Also, recovery of fluorescence in compound 3 was observed, which open interesting opportunities for the development of versatile rhodamine-based probes. © 2017 The Authors}, keywords = {}, pubstate = {published}, tppubtype = {article} } Three rhodamine derivatives exhibiting electrofluorochromic properties were investigated by cyclic voltammetry and UV–Vis/fluorescence spectroelectrochemistry. Rhodamine 101 (Rh101, compound 1) was used as a reference model. In compound 2, the carboxylate anion of Rh101 was replaced by an alkyne moiety to allow further functionalization. The compound 3 was prepared from 2 by conversion of the alkyne to a triazole group bearing an alkyl chain with an alcohol function. These three rhodamine derivatives exhibited similar electrochemical behaviors. Their mono-electronic reductions produced the corresponding radical species which were stable on the time-scale of cyclic voltammetry. Additional reduction of electrogenerated radicals produced unstable anions which underwent subsequent chemical reaction, most likely protonation. Based on cyclic voltammetry investigations, absorption and fluorescence spectroelectrochemistry were then performed on compounds 1, 2, 3 and their parent reduced radicals 1a, 2a, 3a. UV–Vis spectroelectrochemistry, combined with TD-DFT calculation, confirmed the formation of radicals upon mono-electronic reduction of starting rhodamines. Fluorescence spectroelectrochemistry showed that, contrary to their parent molecules, electrogenerated radicals were non-fluorescent. Electrochemical fluorescence extinction was successfully achieved with all studied compounds. Moreover, compound 1 underwent on/off switching between fluorescent and non-fluorescent states repeatedly. Also, recovery of fluorescence in compound 3 was observed, which open interesting opportunities for the development of versatile rhodamine-based probes. © 2017 The Authors |