2021
|
Ultrafast photoreduction dynamics of a new class of CPD photolyases Article de journal Fabien Lacombat; Agathe Espagne; Nadia Dozova; Pascal Plaza; Pavel Müller; Hans-Joachim Emmerich; Martin Saft; Lars-Oliver Essen Photochemical & Photobiological Sciences, 20 (6), p. 733-746, 2021, ISSN: 1474-9092. @article{RN632,
title = {Ultrafast photoreduction dynamics of a new class of CPD photolyases},
author = {Fabien Lacombat and Agathe Espagne and Nadia Dozova and Pascal Plaza and Pavel M\"{u}ller and Hans-Joachim Emmerich and Martin Saft and Lars-Oliver Essen},
url = {https://doi.org/10.1007/s43630-021-00048-4},
doi = {10.1007/s43630-021-00048-4},
issn = {1474-9092},
year = {2021},
date = {2021-01-01},
journal = {Photochemical & Photobiological Sciences},
volume = {20},
number = {6},
pages = {733-746},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Versatile On-Demand Fluorescent Labeling of Fusion Proteins Using Fluorescence-Activating and Absorption-Shifting Tag (FAST) Livre Arnaud Gautier; Ludovic Jullien; Chenge Li; Marie-Aude Plamont; Alison G Tebo; Marion Thauvin; Michel Volovitch; Sophie Vriz Springer US, New York, NY, 2021, ISBN: 978-1-07-161593-5. @book{gautier_versatile_2021,
title = {Versatile On-Demand Fluorescent Labeling of Fusion Proteins Using Fluorescence-Activating and Absorption-Shifting Tag (FAST)},
author = {Arnaud Gautier and Ludovic Jullien and Chenge Li and Marie-Aude Plamont and Alison G Tebo and Marion Thauvin and Michel Volovitch and Sophie Vriz},
editor = {Eli Zamir},
url = {https://doi.org/10.1007/978-1-0716-1593-5_16},
doi = {10.1007/978-1-0716-1593-5_16},
isbn = {978-1-07-161593-5},
year = {2021},
date = {2021-01-01},
urldate = {2023-10-31},
booktitle = {Multiplexed Imaging: Methods and Protocols},
pages = {253--265},
publisher = {Springer US},
address = {New York, NY},
series = {Methods in Molecular Biology},
abstract = {Observing the localization, the concentration, and the distribution of proteins in cells or organisms is essential to understand theirs functions. General and versatile methods allowing multiplexed imaging of proteins under a large variety of experimental conditions are thus essential for deciphering the inner workings of cells and organisms. Here, we present a general method based on the non-covalent labeling of a small protein tag, named FAST (fluorescence-activating and absorption-shifting tag), with various fluorogenic ligands that light up upon labeling, which makes the simple, robust, and versatile on-demand labeling of fusion proteins in a wide range of experimental systems possible.},
keywords = {},
pubstate = {published},
tppubtype = {book}
}
Observing the localization, the concentration, and the distribution of proteins in cells or organisms is essential to understand theirs functions. General and versatile methods allowing multiplexed imaging of proteins under a large variety of experimental conditions are thus essential for deciphering the inner workings of cells and organisms. Here, we present a general method based on the non-covalent labeling of a small protein tag, named FAST (fluorescence-activating and absorption-shifting tag), with various fluorogenic ligands that light up upon labeling, which makes the simple, robust, and versatile on-demand labeling of fusion proteins in a wide range of experimental systems possible. |
2020
|
A Far-Red Emitting Fluorescent Chemogenetic Reporter for In Vivo Molecular Imaging Article de journal Chenge Li; Alison G Tebo; Marion Thauvin; Marie-Aude Plamont; Michel Volovitch; Xavier Morin; Sophie Vriz; Arnaud Gautier Angewandte Chemie International Edition, 59 (41), p. 17917–17923, 2020, ISSN: 1521-3773, (_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1002/anie.202006576). @article{li_far-red_2020,
title = {A Far-Red Emitting Fluorescent Chemogenetic Reporter for In Vivo Molecular Imaging},
author = {Chenge Li and Alison G Tebo and Marion Thauvin and Marie-Aude Plamont and Michel Volovitch and Xavier Morin and Sophie Vriz and Arnaud Gautier},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.202006576},
doi = {10.1002/anie.202006576},
issn = {1521-3773},
year = {2020},
date = {2020-01-01},
urldate = {2023-10-31},
journal = {Angewandte Chemie International Edition},
volume = {59},
number = {41},
pages = {17917--17923},
abstract = {Far-red emitting fluorescent labels are highly desirable for spectral multiplexing and deep tissue imaging. Here, we describe the generation of frFAST (far-red Fluorescence Activating and absorption Shifting Tag), a 14-kDa monomeric protein that forms a bright far-red fluorescent assembly with (4-hydroxy-3-methoxy-phenyl)allylidene rhodanine (HPAR-3OM). As HPAR-3OM is essentially non-fluorescent in solution and in cells, frFAST can be imaged with high contrast in presence of free HPAR-3OM, which allowed the rapid and efficient imaging of frFAST fusions in live cells, zebrafish embryo/larvae, and chicken embryos. Beyond enabling the genetic encoding of far-red fluorescence, frFAST allowed the design of a far-red chemogenetic reporter of protein\textendashprotein interactions, demonstrating its great potential for the design of innovative far-red emitting biosensors.},
note = {_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1002/anie.202006576},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Far-red emitting fluorescent labels are highly desirable for spectral multiplexing and deep tissue imaging. Here, we describe the generation of frFAST (far-red Fluorescence Activating and absorption Shifting Tag), a 14-kDa monomeric protein that forms a bright far-red fluorescent assembly with (4-hydroxy-3-methoxy-phenyl)allylidene rhodanine (HPAR-3OM). As HPAR-3OM is essentially non-fluorescent in solution and in cells, frFAST can be imaged with high contrast in presence of free HPAR-3OM, which allowed the rapid and efficient imaging of frFAST fusions in live cells, zebrafish embryo/larvae, and chicken embryos. Beyond enabling the genetic encoding of far-red fluorescence, frFAST allowed the design of a far-red chemogenetic reporter of protein–protein interactions, demonstrating its great potential for the design of innovative far-red emitting biosensors. |
Disclosing the redox metabolism of drugs: The essential role of electrochemistry Article de journal O Buriez; E Labbe Current Opinion in Electrochemistry, 24 , p. 63-68, 2020. @article{,
title = {Disclosing the redox metabolism of drugs: The essential role of electrochemistry},
author = {O Buriez and E Labbe},
url = {https://doi.org/10.1016/j.coelec.2020.07.002},
doi = {10.1016/j.coelec.2020.07.002},
year = {2020},
date = {2020-12-01},
journal = {Current Opinion in Electrochemistry},
volume = {24},
pages = {63-68},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Disclosing the redox metabolism of drugs: the essential role of electrochemistry Article de journal Olivier Buriez; Eric Labbé Current Opinion in Electrochemistry, 24 , p. 63–68, 2020. @article{buriez2020disclosing,
title = {Disclosing the redox metabolism of drugs: the essential role of electrochemistry},
author = {Olivier Buriez and Eric Labb\'{e}},
year = {2020},
date = {2020-01-01},
journal = {Current Opinion in Electrochemistry},
volume = {24},
pages = {63--68},
publisher = {Elsevier},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Dynamic contrast for overcoming spectral interferences in fluorescence imaging Article de journal R Chouket; A Pellissier-Tanon; A Lemarchand; A Espagne; T Le Saux; L Jullien Journal of Physics-Photonics, 2 (3), p. 8, 2020, ISSN: 2515-7647. @article{RN1_30,
title = {Dynamic contrast for overcoming spectral interferences in fluorescence imaging},
author = {R Chouket and A Pellissier-Tanon and A Lemarchand and A Espagne and T Le Saux and L Jullien},
url = {<Go to ISI>://WOS:000572940200001},
doi = {10.1088/2515-7647/ab9099},
issn = {2515-7647},
year = {2020},
date = {2020-01-01},
journal = {Journal of Physics-Photonics},
volume = {2},
number = {3},
pages = {8},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Dynamic contrast with reversibly photoswitchable fluorescent labels for imaging living cells Article de journal R Chouket; A Pellissier-Tanon; A Lemarchand; A Espagne; T Le Saux; L Jullien Chemical Science, 11 (11), p. 2882-2887, 2020, ISSN: 2041-6520. @article{RN2x,
title = {Dynamic contrast with reversibly photoswitchable fluorescent labels for imaging living cells},
author = {R Chouket and A Pellissier-Tanon and A Lemarchand and A Espagne and T Le Saux and L Jullien},
url = {<Go to ISI>://WOS:000521247400002},
doi = {10.1039/d0sc00182a},
issn = {2041-6520},
year = {2020},
date = {2020-01-01},
journal = {Chemical Science},
volume = {11},
number = {11},
pages = {2882-2887},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Inducible intracellular membranes: molecular aspects and emerging applications Article de journal J Royes; V Biou; N Dautin; C Tribet; B Miroux Microbial Cell Factories, 19 (1), 2020. @article{RN263,
title = {Inducible intracellular membranes: molecular aspects and emerging applications},
author = {J Royes and V Biou and N Dautin and C Tribet and B Miroux},
url = {<Go to ISI>://WOS:000568404600002},
doi = {10.1186/s12934-020-01433-x},
year = {2020},
date = {2020-01-01},
journal = {Microbial Cell Factories},
volume = {19},
number = {1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Mediator-Microorganism Interaction in Microbial Solar Cell: a Fluo-Electrochemical Insight Article de journal L Beauzamy; J Delacotte; B Bailleul; K Tanaka; S Nakanishi; F A Wollman; F Lemaitre Analytical Chemistry, 92 (11), p. 7532-7539, 2020. @article{,
title = {Mediator-Microorganism Interaction in Microbial Solar Cell: a Fluo-Electrochemical Insight},
author = {L Beauzamy and J Delacotte and B Bailleul and K Tanaka and S Nakanishi and F A Wollman and F Lemaitre},
url = {https://doi.org/10.1021/acs.analchem.9b05808},
doi = {10.1021/acs.analchem.9b05808},
year = {2020},
date = {2020-06-01},
journal = {Analytical Chemistry},
volume = {92},
number = {11},
pages = {7532-7539},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Overview and outlook of the strategies devoted to electrofluorescence surveys: Application to single cell secretion analysis Article de journal M Guille-Collignon; F Lemaitre Trac-Trends in Analytical Chemistry, 132 , p. 116055, 2020. @article{,
title = {Overview and outlook of the strategies devoted to electrofluorescence surveys: Application to single cell secretion analysis},
author = {M Guille-Collignon and F Lemaitre},
url = {https://doi.org/10.1016/j.trac.2020.116055},
doi = {10.1016/j.trac.2020.116055},
year = {2020},
date = {2020-11-01},
journal = {Trac-Trends in Analytical Chemistry},
volume = {132},
pages = {116055},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Solubilization and Stabilization of Membrane Proteins by Cycloalkane-Modified Amphiphilic Polymers Article de journal Anaïs Marconnet; Baptiste Michon; Christel Le Bon; Fabrice Giusti; Christophe Tribet; Manuela Zoonens Biomacromolecules, 21 (8), p. 3459-3467, 2020, ISSN: 1525-7797. @article{Marconnet2020,
title = {Solubilization and Stabilization of Membrane Proteins by Cycloalkane-Modified Amphiphilic Polymers},
author = {Ana\"{i}s Marconnet and Baptiste Michon and Christel Le Bon and Fabrice Giusti and Christophe Tribet and Manuela Zoonens },
editor = {American Chemical Society},
url = {https://doi.org/10.1021/acs.biomac.0c00929},
doi = {10.1021/acs.biomac.0c00929},
issn = {1525-7797},
year = {2020},
date = {2020-08-10},
journal = {Biomacromolecules},
volume = {21},
number = {8},
pages = {3459-3467},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
The strong potential of organic and molecular electrochemistry Article de journal O Buriez Current Opinion in Electrochemistry, 24 , p. A1-A3, 2020. @article{,
title = {The strong potential of organic and molecular electrochemistry},
author = {O Buriez},
url = {https://doi.org/10.1016/j.coelec.2020.100657},
doi = {10.1016/j.coelec.2020.100657},
year = {2020},
date = {2020-12-01},
journal = {Current Opinion in Electrochemistry},
volume = {24},
pages = {A1-A3},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Underlying mechanisms in microbial solar cells: how modeling can help Article de journal L Beauzamy; F Lemaitre; J Derr Sustainable Energy & Fuels, 4 (12), p. 6004-6010, 2020. @article{,
title = {Underlying mechanisms in microbial solar cells: how modeling can help},
author = {L Beauzamy and F Lemaitre and J Derr},
url = {https://doi.org/10.1039/d0se01304h},
doi = {10.1039/d0se01304h},
year = {2020},
date = {2020-12-01},
journal = {Sustainable Energy & Fuels},
volume = {4},
number = {12},
pages = {6004-6010},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2019
|
A fluorescent false neurotransmitter as a dual electrofluorescent probe for secretory cell models Article de journal Justine Pandard; Na Pan; Dina H Ebene; Thomas Le Saux; Eric Ait-Yahiat`ene; Xiaoqing Liu; Laurence Grimaud; Olivier Buriez; Eric Labbé; Frédéric Lema^itre; others ChemPlusChem, 84 (10), p. 1578–1586, 2019. @article{pandard2019fluorescent,
title = {A fluorescent false neurotransmitter as a dual electrofluorescent probe for secretory cell models},
author = {Justine Pandard and Na Pan and Dina H Ebene and Thomas Le Saux and Eric Ait-Yahiat{`e}ne and Xiaoqing Liu and Laurence Grimaud and Olivier Buriez and Eric Labb\'{e} and Fr\'{e}d\'{e}ric Lema{^i}tre and others},
year = {2019},
date = {2019-01-01},
journal = {ChemPlusChem},
volume = {84},
number = {10},
pages = {1578--1586},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
A Fluorescent False Neurotransmitter as a Dual Electrofluorescent Probe for Secretory Cell Models Article de journal J Pandard; N Pan; D H Ebene; T Le Saux; E Ait-Yahiatène; X Liu; L Grimaud; O Buriez; E Labbé; F Lemaître; M Guille-Collignon ChemPlusChem, 84 (10), p. 1578-1586, 2019, (cited By 0). @article{Pandard20191578,
title = {A Fluorescent False Neurotransmitter as a Dual Electrofluorescent Probe for Secretory Cell Models},
author = {J Pandard and N Pan and D H Ebene and T Le Saux and E Ait-Yahiat\`{e}ne and X Liu and L Grimaud and O Buriez and E Labb\'{e} and F Lema\^{i}tre and M Guille-Collignon},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85073931778&doi=10.1002%2fcplu.201900385&partnerID=40&md5=a526291d966e7dc926b44e97c73794b3},
doi = {10.1002/cplu.201900385},
year = {2019},
date = {2019-01-01},
journal = {ChemPlusChem},
volume = {84},
number = {10},
pages = {1578-1586},
note = {cited By 0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
A split fluorescent reporter with rapid and reversible complementation Article de journal Alison G Tebo; Arnaud Gautier Nature Communications, 10 (1), p. 2822, 2019, ISSN: 2041-1723. @article{Tebo2019,
title = {A split fluorescent reporter with rapid and reversible complementation},
author = {Alison G Tebo and Arnaud Gautier},
url = {https://doi.org/10.1038/s41467-019-10855-0},
doi = {10.1038/s41467-019-10855-0},
issn = {2041-1723},
year = {2019},
date = {2019-01-01},
journal = {Nature Communications},
volume = {10},
number = {1},
pages = {2822},
abstract = {Interactions between proteins play an essential role in metabolic and signaling pathways, cellular processes and organismal systems. We report the development of splitFAST, a fluorescence complementation system for the visualization of transient protein-protein interactions in living cells. Engineered from the fluorogenic reporter FAST (Fluorescence-Activating and absorption-Shifting Tag), which specifically and reversibly binds fluorogenic hydroxybenzylidene rhodanine (HBR) analogs, splitFAST displays rapid and reversible complementation, allowing the real-time visualization of both the formation and the dissociation of a protein assembly.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Interactions between proteins play an essential role in metabolic and signaling pathways, cellular processes and organismal systems. We report the development of splitFAST, a fluorescence complementation system for the visualization of transient protein-protein interactions in living cells. Engineered from the fluorogenic reporter FAST (Fluorescence-Activating and absorption-Shifting Tag), which specifically and reversibly binds fluorogenic hydroxybenzylidene rhodanine (HBR) analogs, splitFAST displays rapid and reversible complementation, allowing the real-time visualization of both the formation and the dissociation of a protein assembly. |
Bacteria-Based Production of Thiol-Clickable, Genetically Encoded Lipid Nanovesicles Article de journal Jorge Royes; Oana Ilioaia; Quentin Lubart; Federica Angius; Galina V Dubacheva; Marta Bally; Bruno Miroux; Christophe Tribet Angewandte Chemie International Edition, 58 , p. 7395-7399, 2019, ISSN: 1433-7851. @article{RN1x,
title = {Bacteria-Based Production of Thiol-Clickable, Genetically Encoded Lipid Nanovesicles},
author = {Jorge Royes and Oana Ilioaia and Quentin Lubart and Federica Angius and Galina V Dubacheva and Marta Bally and Bruno Miroux and Christophe Tribet},
doi = {10.1002/anie.201902929},
issn = {1433-7851},
year = {2019},
date = {2019-01-01},
journal = {Angewandte Chemie International Edition},
volume = {58},
pages = {7395-7399},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Chapter One - Optical control of protein activity and gene expression by photoactivation of caged cyclofen Livre Fatima Hamouri; Weiting Zhang; Isabelle Aujard; Thomas Le Saux; Bertrand Ducos; Sophie Vriz; Ludovic Jullien; David Bensimon Academic Press, 2019. @book{hamouri_chapter_2019,
title = {Chapter One - Optical control of protein activity and gene expression by photoactivation of caged cyclofen},
author = {Fatima Hamouri and Weiting Zhang and Isabelle Aujard and Thomas Le Saux and Bertrand Ducos and Sophie Vriz and Ludovic Jullien and David Bensimon},
editor = {Alexander Deiters},
url = {https://www.sciencedirect.com/science/article/pii/S0076687919301247},
doi = {10.1016/bs.mie.2019.04.009},
year = {2019},
date = {2019-01-01},
urldate = {2023-10-31},
booktitle = {Methods in Enzymology},
volume = {624},
pages = {1--23},
publisher = {Academic Press},
series = {Optochemical Biology},
abstract = {The use of light to control the expression of genes and the activity of proteins is a rapidly expanding field. While many of these approaches use a fusion between a light activatable protein and the protein of interest to control the activity of the latter, it is also possible to control the activity of a protein by uncaging a specific ligand. In that context, controlling the activation of a protein fused to the modified estrogen receptor (ERT) by uncaging its ligand cyclofen-OH has emerged as a generic and versatile method to control the activation of proteins quantitatively, quickly and locally in a live organism. Here, we present the experimental details behind this approach.},
keywords = {},
pubstate = {published},
tppubtype = {book}
}
The use of light to control the expression of genes and the activity of proteins is a rapidly expanding field. While many of these approaches use a fusion between a light activatable protein and the protein of interest to control the activity of the latter, it is also possible to control the activity of a protein by uncaging a specific ligand. In that context, controlling the activation of a protein fused to the modified estrogen receptor (ERT) by uncaging its ligand cyclofen-OH has emerged as a generic and versatile method to control the activation of proteins quantitatively, quickly and locally in a live organism. Here, we present the experimental details behind this approach. |
Cover Feature: Fundamental Input of Analytical Electrochemistry in the Determination of Intermediates and Reaction Mechanisms in Electrosynthetic Processes Article de journal E Labbe; O Buriez Chemelectrochem, 6 (16), p. 4061, 2019. @article{,
title = {Cover Feature: Fundamental Input of Analytical Electrochemistry in the Determination of Intermediates and Reaction Mechanisms in Electrosynthetic Processes},
author = {E Labbe and O Buriez},
url = {https://doi.org/10.1002/celc.201901158},
doi = {10.1002/celc.201901158},
year = {2019},
date = {2019-01-01},
journal = {Chemelectrochem},
volume = {6},
number = {16},
pages = {4061},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Cover Feature: Fundamental Input of Analytical Electrochemistry in the Determination of Intermediates and Reaction Mechanisms in Electrosynthetic Processes (ChemElectroChem 16/2019). Article de journal Eric Labbé; Olivier Buriez ChemElectroChem, 6 (16), 2019. @article{labbe2019cover,
title = {Cover Feature: Fundamental Input of Analytical Electrochemistry in the Determination of Intermediates and Reaction Mechanisms in Electrosynthetic Processes (ChemElectroChem 16/2019).},
author = {Eric Labb\'{e} and Olivier Buriez},
year = {2019},
date = {2019-01-01},
journal = {ChemElectroChem},
volume = {6},
number = {16},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Distinctive Low-Resolution Structural Features of Dimers of Antibody–Drug Conjugates and Parent Antibody Determined by Small-Angle X-ray Scattering Article de journal Didier Law-Hine; Sergii Rudiuk; Audrey Bonestebe; Romain Ienco; Sylvain Huille; Christophe Tribet Mol. Pharmaceutics, 16 (12), p. 4902-4912, 2019. @article{Law-Hine2019,
title = {Distinctive Low-Resolution Structural Features of Dimers of Antibody\textendashDrug Conjugates and Parent Antibody Determined by Small-Angle X-ray Scattering},
author = {Didier Law-Hine and Sergii Rudiuk and Audrey Bonestebe and Romain Ienco and Sylvain Huille and Christophe Tribet},
doi = {10.1021/acs.molpharmaceut.9b00792},
year = {2019},
date = {2019-10-16},
journal = {Mol. Pharmaceutics},
volume = {16},
number = {12},
pages = {4902-4912},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Diverting photosynthetic electrons from suspensions of Chlamydomonas reinhardtii algae - New insights using an electrochemical well device Article de journal A Sayegh; G Longatte; O Buriez; F -A Wollman; M Guille-Collignon; E Labbé; J Delacotte; F Lemaître Electrochimica Acta, 304 , p. 465 - 473, 2019, ISSN: 0013-4686. @article{SAYEGH2019465,
title = {Diverting photosynthetic electrons from suspensions of Chlamydomonas reinhardtii algae - New insights using an electrochemical well device},
author = {A Sayegh and G Longatte and O Buriez and F -A Wollman and M Guille-Collignon and E Labb\'{e} and J Delacotte and F Lema\^{i}tre},
url = {http://www.sciencedirect.com/science/article/pii/S0013468619303718},
doi = {https://doi.org/10.1016/j.electacta.2019.02.105},
issn = {0013-4686},
year = {2019},
date = {2019-01-01},
journal = {Electrochimica Acta},
volume = {304},
pages = {465 - 473},
abstract = {In the last years, many strategies have been developed to benefit from oxygenic photosynthesis in the present context of renewable energies. To achieve this, bioelectricity may be produced by using photosynthetic components involved in anodic or cathodic compartments. In this respect, harvesting photosynthetic electrons from living biological systems appears to be an encouraging approach. However it raises the question of the most suitable electrochemical device. In this work, we describe and analyze the performances of an electrochemical device based on a millimeter sized well involving a gold surface as a working electrode. Photocurrents were generated by suspensions of Chlamydomonas reinhardtii algae using quinones as mediators under different experimental conditions. Chronoamperometry and cyclic voltammetry measurements gave insight into the use of this device to investigate important issues (harvesting and poisoning by quinones, photoinactivation…). Furthermore, by introducing a kinetic model originally developed for homogeneous catalytic systems, the kinetics of the electron diverting from this system (Chlamydomonas reinhardtii algae + 2,6-DCBQ + miniaturized setup) can be estimated. All these results demonstrate that this experimental configuration is suitable for future works devoted to the choice of the best parameters in terms of long lasting performances.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In the last years, many strategies have been developed to benefit from oxygenic photosynthesis in the present context of renewable energies. To achieve this, bioelectricity may be produced by using photosynthetic components involved in anodic or cathodic compartments. In this respect, harvesting photosynthetic electrons from living biological systems appears to be an encouraging approach. However it raises the question of the most suitable electrochemical device. In this work, we describe and analyze the performances of an electrochemical device based on a millimeter sized well involving a gold surface as a working electrode. Photocurrents were generated by suspensions of Chlamydomonas reinhardtii algae using quinones as mediators under different experimental conditions. Chronoamperometry and cyclic voltammetry measurements gave insight into the use of this device to investigate important issues (harvesting and poisoning by quinones, photoinactivation…). Furthermore, by introducing a kinetic model originally developed for homogeneous catalytic systems, the kinetics of the electron diverting from this system (Chlamydomonas reinhardtii algae + 2,6-DCBQ + miniaturized setup) can be estimated. All these results demonstrate that this experimental configuration is suitable for future works devoted to the choice of the best parameters in terms of long lasting performances. |
Diverting photosynthetic electrons from suspensions of Chlamydomonas reinhardtii algae-New insights using an electrochemical well device Article de journal Adnan Sayegh; Guillaume Longatte; Olivier Buriez; Francis-André Wollman; Manon Guille-Collignon; Eric Labbé; Jér^ome Delacotte; Frédéric Lema^itre Electrochimica Acta, 304 , p. 465–473, 2019. @article{sayegh2019diverting,
title = {Diverting photosynthetic electrons from suspensions of Chlamydomonas reinhardtii algae-New insights using an electrochemical well device},
author = {Adnan Sayegh and Guillaume Longatte and Olivier Buriez and Francis-Andr\'{e} Wollman and Manon Guille-Collignon and Eric Labb\'{e} and J\'{e}r{^o}me Delacotte and Fr\'{e}d\'{e}ric Lema{^i}tre},
year = {2019},
date = {2019-01-01},
journal = {Electrochimica Acta},
volume = {304},
pages = {465--473},
publisher = {Elsevier},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Electroactive fluorescent false neurotransmitter FFN102 partially replaces dopamine in PC12 cell vesicles Article de journal L Hu; A Savy; L Grimaud; M Guille-Collignon; F Lemaître; C Amatore; J Delacotte Biophysical Chemistry, 245 , p. 1–5, 2019. @article{Hu:2019,
title = {Electroactive fluorescent false neurotransmitter FFN102 partially replaces dopamine in PC12 cell vesicles},
author = {L Hu and A Savy and L Grimaud and M Guille-Collignon and F Lema\^{i}tre and C Amatore and J Delacotte},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85057201704&doi=10.1016%2fj.bpc.2018.11.001&partnerID=40&md5=28655b4c152ce0fc51fc037feefcff97},
doi = {10.1016/j.bpc.2018.11.001},
year = {2019},
date = {2019-01-01},
journal = {Biophysical Chemistry},
volume = {245},
pages = {1--5},
abstract = {In the last decade, following fluorescent dyes and protein tags, pH sensitive false fluorescent neurotransmitters (FFN) were introduced and were valuable for labeling secretory vesicles and monitoring exocytosis at living cells. In particular, the synthetic analog of neurotransmitters FFN102 was shown to be an electroactive probe. Here, we show that FFN102 is suitable to be used as a bioanalytic probe at the widely used PC12 cell model. FFN102 was uptaken in the secretory vesicles of PC12 cells, partially replacing the endogenous dopamine stored in these vesicles. The different oxidation potentials of dopamine and FFN102 allowed to determine that ca. 12% of dopamine was replaced by FFN102. Moreover, the FFN102 was found to be over released through the initial fusion pore suggesting that it was mostly uptaken in fast diffusion compartment of the vesicles. © 2018 Elsevier B.V.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In the last decade, following fluorescent dyes and protein tags, pH sensitive false fluorescent neurotransmitters (FFN) were introduced and were valuable for labeling secretory vesicles and monitoring exocytosis at living cells. In particular, the synthetic analog of neurotransmitters FFN102 was shown to be an electroactive probe. Here, we show that FFN102 is suitable to be used as a bioanalytic probe at the widely used PC12 cell model. FFN102 was uptaken in the secretory vesicles of PC12 cells, partially replacing the endogenous dopamine stored in these vesicles. The different oxidation potentials of dopamine and FFN102 allowed to determine that ca. 12% of dopamine was replaced by FFN102. Moreover, the FFN102 was found to be over released through the initial fusion pore suggesting that it was mostly uptaken in fast diffusion compartment of the vesicles. © 2018 Elsevier B.V. |
Fundamental Input of Analytical Electrochemistry in the Determination of Intermediates and Reaction Mechanisms in Electrosynthetic Processes Article de journal E Labbé; O Buriez ChemElectroChem, 6 (16), p. 4118-4125, 2019, (cited By 1). @article{Labb\'{e}20194118,
title = {Fundamental Input of Analytical Electrochemistry in the Determination of Intermediates and Reaction Mechanisms in Electrosynthetic Processes},
author = {E Labb\'{e} and O Buriez},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85063908009&doi=10.1002%2fcelc.201900045&partnerID=40&md5=be86c0958f347ea6b4d1d62a0526e44c},
doi = {10.1002/celc.201900045},
year = {2019},
date = {2019-01-01},
journal = {ChemElectroChem},
volume = {6},
number = {16},
pages = {4118-4125},
note = {cited By 1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Fundamental input of analytical electrochemistry in the determination of intermediates and reaction mechanisms in electrosynthetic processes Article de journal Eric Labbé; Olivier Buriez ChemElectroChem, 6 (16), p. 4118–4125, 2019. @article{labbe2019fundamental,
title = {Fundamental input of analytical electrochemistry in the determination of intermediates and reaction mechanisms in electrosynthetic processes},
author = {Eric Labb\'{e} and Olivier Buriez},
year = {2019},
date = {2019-01-01},
journal = {ChemElectroChem},
volume = {6},
number = {16},
pages = {4118--4125},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Live Cell Super Resolution Imaging by Radial Fluctuations Using Fluorogen Binding Tags Article de journal Muthukumaran Venkatachalapathy; Vivek Belapurkar; Mini Jose; Arnaud Gautier; Deepak Nair Nanoscale, 11 (8), p. 3626-3632, 2019, ISSN: 2040-3364. @article{RN45,
title = {Live Cell Super Resolution Imaging by Radial Fluctuations Using Fluorogen Binding Tags},
author = {Muthukumaran Venkatachalapathy and Vivek Belapurkar and Mini Jose and Arnaud Gautier and Deepak Nair},
doi = {10.1039/C8NR07809B},
issn = {2040-3364},
year = {2019},
date = {2019-01-01},
journal = {Nanoscale},
volume = {11},
number = {8},
pages = {3626-3632},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Nanoparticle-based local translation reveals mRNA as a translation-coupled scaffold with anchoring function Article de journal Shunnichi Kashida; Dan Ohtan Wang; Hirohide Saito; Zoher Gueroui Proceedings of the National Academy of Sciences of the United States of America, 2019, ISSN: 10916490. @article{Kashida2019,
title = {Nanoparticle-based local translation reveals mRNA as a translation-coupled scaffold with anchoring function},
author = {Shunnichi Kashida and Dan Ohtan Wang and Hirohide Saito and Zoher Gueroui},
doi = {10.1073/pnas.1900310116},
issn = {10916490},
year = {2019},
date = {2019-01-01},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
abstract = {The spatial regulation of messenger RNA (mRNA) translation is central to cellular functions and relies on numerous complex processes. Biomimetic approaches could bypass these endogenous complex processes, improve our comprehension of the regulation, and allow for controlling local translation regulations and functions. However, the causality between local translation and nascent protein function remains elusive. Here, we developed a nanoparticle (NP)-based strategy to magnetically control mRNA spatial patterns in mammalian cell extracts and investigate how local translation impacts nascent protein localization and function. By monitoring the translation of the magnetically localized mRNAs, we show that mRNA\textendashNP complexes operate as a source for the continuous production of proteins from defined positions. By applying this approach to actin-binding proteins, we triggered the local formation of actin cytoskeletons and identified the minimal requirements for spatial control of the actin filament network. In addition, our bottom-up approach identified a role for mRNA as a translation-coupled scaffold for the function of nascent N-terminal protein domains. Our approach will serve as a platform for regulating mRNA localization and investigating the function of nascent protein domains during translation.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The spatial regulation of messenger RNA (mRNA) translation is central to cellular functions and relies on numerous complex processes. Biomimetic approaches could bypass these endogenous complex processes, improve our comprehension of the regulation, and allow for controlling local translation regulations and functions. However, the causality between local translation and nascent protein function remains elusive. Here, we developed a nanoparticle (NP)-based strategy to magnetically control mRNA spatial patterns in mammalian cell extracts and investigate how local translation impacts nascent protein localization and function. By monitoring the translation of the magnetically localized mRNAs, we show that mRNA–NP complexes operate as a source for the continuous production of proteins from defined positions. By applying this approach to actin-binding proteins, we triggered the local formation of actin cytoskeletons and identified the minimal requirements for spatial control of the actin filament network. In addition, our bottom-up approach identified a role for mRNA as a translation-coupled scaffold for the function of nascent N-terminal protein domains. Our approach will serve as a platform for regulating mRNA localization and investigating the function of nascent protein domains during translation. |
New Mechanistic Insights into Osmium-Based Tamoxifen Derivatives Article de journal H Z S Lee; F Chau; S Top; G Jaouen; A Vessieres; E Labbe; O Buriez Electrochimica Acta, 302 , p. 130-136, 2019, ISSN: 0013-4686. @article{RN52,
title = {New Mechanistic Insights into Osmium-Based Tamoxifen Derivatives},
author = {H Z S Lee and F Chau and S Top and G Jaouen and A Vessieres and E Labbe and O Buriez},
doi = {10.1016/j.electacta.2019.02.019},
issn = {0013-4686},
year = {2019},
date = {2019-01-01},
journal = {Electrochimica Acta},
volume = {302},
pages = {130-136},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
New mechanistic insights into osmium-based tamoxifen derivatives Article de journal Hui Zhi Shirley Lee; François Chau; Siden Top; Gérard Jaouen; Anne Vessi`eres; Eric Labbé; Olivier Buriez Electrochimica Acta, 302 , p. 130–136, 2019. @article{lee2019new,
title = {New mechanistic insights into osmium-based tamoxifen derivatives},
author = {Hui Zhi Shirley Lee and Fran{\c{c}}ois Chau and Siden Top and G\'{e}rard Jaouen and Anne Vessi{`e}res and Eric Labb\'{e} and Olivier Buriez},
year = {2019},
date = {2019-01-01},
journal = {Electrochimica Acta},
volume = {302},
pages = {130--136},
publisher = {Elsevier},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
