Nicolas BOLIK-COULON

PhD student, École Normale Supérieure
Laboratoire des Biomolécules, UMR7203

ENS – Département de chimie
24 rue Lhomond, 75005 Paris

Email: nicolas.bolik-coulon@ens.psl.eu
Office: ES111

Short bio

Nicolas Bolik-Coulon was an undergrad at the Chemistry department of the École Normale Supérieure, Paris, where he received training in organic, inorganic and physical chemistry and then specialized in biophysics. He joined the Laboratoire des Biomolecules in 2016 for his master thesis under the supervision of Prof. Fabien Ferrage on the characterization of the structural ensemble of a long Intrinsically Disordered Protein (IDP) using Nuclear Magnetic Resonance (NMR). As part of his program, he stayed another year in the lab developing computational tools to analyze high-resolution relaxometry data to characterize the dynamics of proteins over a wide range of timescales (pico- to nanosecond). In 2017-2018, he joined Prof. Wolfgrang Peti at the University of Arizona, Tucson, USA. He started his PhD under the supervision of Prof. Fabien Ferrage in 2018 on the development of tools to characterize the dynamics of protein by NMR.

Education and professional experience

  • since 2018               PhD student under the supervision of Prof. Fabien Ferrage, Ecole Normale Supérieure, Paris
  • 2019-2021               Co-chair of the Gordon Research Seminar on Computational Aspects of Biomolecular NMR
  • 2017-2018               Research Technician in Prof. Wolfgang Peti lab, University of Arizona, USA
  • 2016-2017               Internship student under the supervision of Prof. Fabien Ferrage, Ecole Normale Supérieure, Paris
  • 2014-2016               Master of Science in Chemistry and Biophysics, with distinction
  • 2011-2014               Bachelor of Science in Chemistry, with distinction

Research interests

  • Protein dynamics
  • Nuclear Magnetic Resonance
  • Structural biology

Awards and distinctions

  • 2018-2021             PhD fellowship from the École Normale Supérieure, Paris
  • 2013-2017              4-year fellowship from the École Normale Supérieure, Paris

Teaching

  • Teaching Assistant at the Chemistry Department of the École Normale Supérieure

Significant publications

  1. Time-Resolved Protein Side-Chain Motions Unraveled by High-resolution Relaxometry and Molecular Dynamics Simulations, S.F. Cousin, P. Kadeřávek, N. Bolik-Coulon, Y. Gu, C. Charlier, L. Carlier, L. Bruschweiler-Li, T. Marquardsen, J-M Tybrun, R. Bruschweiler, F. Ferrage, J. Am. Chem. Soc., 140, 13456 (2018)

Publications

2020

Theoretical and computational framework for the analysis of the relaxation properties of arbitrary spin systems. Application to high-resolution relaxometry Article de journal

N Bolik-Coulon; P Kadeřávek; P Pelupessy; J-N Dumez; F Ferrage; S F Cousin

Journal of Magnetic Resonance, 313 , p. 106718, 2020.

Résumé | Liens

Boosting the resolution of low‐field 15N relaxation experiments on intrinsically disordered proteins with triple‐resonance NMR Article de journal

Z Jasenáková; V Zapletal; P Padrta; M Zachrdla; N Bolik-Coulon; T Marquardsen; J-M Tyburn; L Zidek; F Ferrage

Journal of Biomolecular NMR, 74 , p. 139, 2020.

Résumé | Liens

2019

Protein Dynamics from Accurate Low-Field Site-Specific Longitudinal and Transverse Nuclear Spin Relaxation Article de journal

P Kadeřávek; N Bolik-Coulon; S F Cousin; T Marquardsen; J-M Tyburn; J-N Dumez; F Ferrage

The Journal of Physical Chemistry Letters, 10 , p. 5917, 2019.

Liens

Experimental characterization of the dynamics of IDPs and IDRs by NMR Livre

N Bolik-Coulon; G Bouvignies; L Carlier; F Ferrage

2019, ISBN: 9780128163481.

Liens

Understanding the methyl-TROSY effect over a wide range of magnetic fields Article de journal

N Bolik-Coulon; S F Cousin; P Kadeřávek; J-N Dumez; F Ferrage

The Journal of Chemical Physics, 150 , p. 224202, 2019.

Résumé | Liens

2018

The structure of SDS22 provides insights into the mechanism of heterodimer formation with PP1 Article de journal

M.S. Choy; N Bolik-Coulon; T.L. Archuleta; W. Peti; R. Page

Acta Crystallographica Section F, F74 , p. 817, 2018.

Résumé | Liens

Time-Resolved Protein Side-Chain Motions Unraveled by High-Resolution Relaxometry and Molecular Dynamics Simulations Article de journal

S F Cousin; P Kadeřávek; N Bolik-Coulon; Y Gu; C Charlier; L Carlier; L Bruschweiler-Li; T Marquardsen; J -M Tyburn; R Brüschweiler; F Ferrage

Journal of the American Chemical Society, 140 (41), p. 13456–13465, 2018.

Résumé | Liens

Determination of protein ps-ns motions by high-resolution relaxometry Livre

S F Cousin; P Kadeřávek; N Bolik-Coulon; F Ferrage

2018.

Résumé | Liens

2017

Caspase-6 Undergoes a Distinct Helix-Strand Interconversion upon Substrate Binding Article de journal

K.B. Dagbay; N Bolik-Coulon; S.N. Savinov; J.A. Hardy

Journal of Biological Chemistry, 292 (12), p. 4885, 2017.

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Structure and Dynamics of an Intrinsically Disordered Protein Region That Partially Folds upon Binding by Chemical-Exchange NMR Article de journal

C Charlier; G Bouvignies; P Pelupessy; A Walrant; R Marquant; M Kozlov; P De Ioannes; N Bolik-Coulon; S Sagan; P Cortes; A K Aggarwal; L Carlier; F Ferrage

Journal of the American Chemical Society, 139 (35), p. 12219–12227, 2017.

Résumé | Liens