2013
|
Synchrotron radiation FTIR detection of a metal-carbonyl tamoxifen analog. Correlation with luminescence microscopy to study its subcellular distribution Article de journal S Clède; F Lambert; C Sandt; Z Gueroui; N Delsuc; P Dumas; A Vessières; C Policar Biotechnology Advances, 31 (3), p. 393–395, 2013. @article{Clede:2013,
title = {Synchrotron radiation FTIR detection of a metal-carbonyl tamoxifen analog. Correlation with luminescence microscopy to study its subcellular distribution},
author = {S Cl\`{e}de and F Lambert and C Sandt and Z Gueroui and N Delsuc and P Dumas and A Vessi\`{e}res and C Policar},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875055388&doi=10.1016%2fj.biotechadv.2012.01.023&partnerID=40&md5=064b36e2db4e1260e17d3abc8b07bbd6},
doi = {10.1016/j.biotechadv.2012.01.023},
year = {2013},
date = {2013-01-01},
journal = {Biotechnology Advances},
volume = {31},
number = {3},
pages = {393--395},
abstract = {1,1-Di(4-hydroxyphenyl)-2-cyrhetrenylbut-1-ene 1 is an organometallic conjugate where a [(Cp)Re(CO)3] unit is linked to a hydroxytamoxifen-like structure. Its subcellular nuclear distribution was previously observed in a single cell using the near-field technique AFMIR. We show here that synchrotron radiation FTIR spectromicroscopy (SR-FTIR-SM) enabled the mapping of 1 based on its IR-signature (characteristic bands in the 1850-2200cm-1 range) and pointed out the colocalization of 1 with an area of high amide density. Fluorescence microscopy using DAPI staining performed on the same cells confirmed that this area corresponds to the cell nucleus. © 2012 Elsevier Inc.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
1,1-Di(4-hydroxyphenyl)-2-cyrhetrenylbut-1-ene 1 is an organometallic conjugate where a [(Cp)Re(CO)3] unit is linked to a hydroxytamoxifen-like structure. Its subcellular nuclear distribution was previously observed in a single cell using the near-field technique AFMIR. We show here that synchrotron radiation FTIR spectromicroscopy (SR-FTIR-SM) enabled the mapping of 1 based on its IR-signature (characteristic bands in the 1850-2200cm-1 range) and pointed out the colocalization of 1 with an area of high amide density. Fluorescence microscopy using DAPI staining performed on the same cells confirmed that this area corresponds to the cell nucleus. © 2012 Elsevier Inc. |
2011
|
Relative helix-helix conformations in branched aromatic oligoamide foldamers Article de journal N Delsuc; S Massip; J -M Léger; B Kauffmann; I Huc Journal of the American Chemical Society, 133 (9), p. 3165–3172, 2011. @article{Delsuc:2011,
title = {Relative helix-helix conformations in branched aromatic oligoamide foldamers},
author = {N Delsuc and S Massip and J -M L\'{e}ger and B Kauffmann and I Huc},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79952263733&doi=10.1021%2fja110677a&partnerID=40&md5=36f1e84ade60bac4aee1c82b34b28b99},
doi = {10.1021/ja110677a},
year = {2011},
date = {2011-01-01},
journal = {Journal of the American Chemical Society},
volume = {133},
number = {9},
pages = {3165--3172},
abstract = {The de novo design and synthesis of large and well-organized, tertiary-like, α-peptidic folded architectures is difficult because it relies on multiple cooperative interactions within and between secondary folded motifs of relatively weak intrinsic stability. The very stable helical structures of oligoamides of 8-amino-2-quinoline carboxylic acid offer a way to circumvent this difficulty thanks to their ability to fold into predictable and stable secondary motifs. Branched architectures comprised of two pairs of tetrameric (1), pentameric (2), or octameric (3) oligomers connected via an ethylene glycol spacer were designed and synthesized. The short spacer holds two helices in close proximity, thus enabling interactions between them. Degrees of freedom allowed in the system are well-defined: the relative P or M handedness of the two helices; the relative orientation of the helix axes; and the gauche or anti conformation of the ethylene spacer. Investigating the structures of 1-3 in the solid state and in solution allowed a detailed picture to be drawn of their conformational preferences and dynamics. The high variability of the solid state structures provides many snapshots of possible solution conformations. Helix-helix handedness communication was evidenced and shown to depend both on solvent and on a defined set of side chains at the helix-helix interface. Interdigitation of the side chains was found to restrict free rotation about the ethylene spacer. One solid state structure shows a high level of symmetry and provides a firm basis to further design specific side chain/side chain directional interactions. © 2011 American Chemical Society.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The de novo design and synthesis of large and well-organized, tertiary-like, α-peptidic folded architectures is difficult because it relies on multiple cooperative interactions within and between secondary folded motifs of relatively weak intrinsic stability. The very stable helical structures of oligoamides of 8-amino-2-quinoline carboxylic acid offer a way to circumvent this difficulty thanks to their ability to fold into predictable and stable secondary motifs. Branched architectures comprised of two pairs of tetrameric (1), pentameric (2), or octameric (3) oligomers connected via an ethylene glycol spacer were designed and synthesized. The short spacer holds two helices in close proximity, thus enabling interactions between them. Degrees of freedom allowed in the system are well-defined: the relative P or M handedness of the two helices; the relative orientation of the helix axes; and the gauche or anti conformation of the ethylene spacer. Investigating the structures of 1-3 in the solid state and in solution allowed a detailed picture to be drawn of their conformational preferences and dynamics. The high variability of the solid state structures provides many snapshots of possible solution conformations. Helix-helix handedness communication was evidenced and shown to depend both on solvent and on a defined set of side chains at the helix-helix interface. Interdigitation of the side chains was found to restrict free rotation about the ethylene spacer. One solid state structure shows a high level of symmetry and provides a firm basis to further design specific side chain/side chain directional interactions. © 2011 American Chemical Society. |
2010
|
Cascading transformations within a dynamic self-assembled system Article de journal Victoria E Campbell; Xavier de Hatten; Nicolas Delsuc; Brice Kauffmann; Ivan Huc; Jonathan R Nitschke Nature Chemistry, 2 (8), p. 684–687, 2010, ISSN: 1755-4349. @article{campbell_cascading_2010,
title = {Cascading transformations within a dynamic self-assembled system},
author = {Victoria E Campbell and Xavier de Hatten and Nicolas Delsuc and Brice Kauffmann and Ivan Huc and Jonathan R Nitschke},
url = {https://www.nature.com/articles/nchem.693},
doi = {10.1038/nchem.693},
issn = {1755-4349},
year = {2010},
date = {2010-01-01},
urldate = {2018-03-06},
journal = {Nature Chemistry},
volume = {2},
number = {8},
pages = {684--687},
abstract = {Molecular subcomponents such as phosphate groups are often passed between biomolecules during complex signalling cascades, the flow of which define the motion of the machinery of life. Here, we show how an abiological system consisting of organic subcomponents knitted together by metal-ion coordination can respond to simple signals in complex ways. A CuI3 helicate transformed into its ZnII2CuI analogue following the addition of zinc(II), and the ejected copper(I) went on to induce the self-assembly of a CuI2 helicate from other free subcomponents present in solution. The addition of an additional subcomponent, 8-aminoquinoline, resulted in the formation of a third, more stable CuI3 helicate, requiring the destruction of both the ZnII2CuI and CuI2 helicates to scavenge sufficient CuI for the new structure. This system thus demonstrates two examples in which the application of one signal provokes two distinct responses involving the creation or destruction of complex assemblies as the system seeks thermodynamic equilibrium following perturbation.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Molecular subcomponents such as phosphate groups are often passed between biomolecules during complex signalling cascades, the flow of which define the motion of the machinery of life. Here, we show how an abiological system consisting of organic subcomponents knitted together by metal-ion coordination can respond to simple signals in complex ways. A CuI3 helicate transformed into its ZnII2CuI analogue following the addition of zinc(II), and the ejected copper(I) went on to induce the self-assembly of a CuI2 helicate from other free subcomponents present in solution. The addition of an additional subcomponent, 8-aminoquinoline, resulted in the formation of a third, more stable CuI3 helicate, requiring the destruction of both the ZnII2CuI and CuI2 helicates to scavenge sufficient CuI for the new structure. This system thus demonstrates two examples in which the application of one signal provokes two distinct responses involving the creation or destruction of complex assemblies as the system seeks thermodynamic equilibrium following perturbation. |
2009
|
Helical Aromatic Oligoamide Foldamers as Organizational Scaffolds for Photoinduced Charge Transfer Article de journal Martin Wolffs; Nicolas Delsuc; Dirk Veldman; Nguyễn Vân Anh; René M Williams; Stefan C J Meskers; René A J Janssen; Ivan Huc; Albertus P H J Schenning Journal of the American Chemical Society, 131 (13), p. 4819–4829, 2009, ISSN: 0002-7863, 1520-5126. @article{wolffs_helical_2009,
title = {Helical Aromatic Oligoamide Foldamers as Organizational Scaffolds for Photoinduced Charge Transfer},
author = {Martin Wolffs and Nicolas Delsuc and Dirk Veldman and Nguyễn V\^{a}n Anh and Ren\'{e} M Williams and Stefan C J Meskers and Ren\'{e} A J Janssen and Ivan Huc and Albertus P H J Schenning},
url = {http://pubs.acs.org/doi/abs/10.1021/ja809367u},
doi = {10.1021/ja809367u},
issn = {0002-7863, 1520-5126},
year = {2009},
date = {2009-01-01},
urldate = {2018-02-18},
journal = {Journal of the American Chemical Society},
volume = {131},
number = {13},
pages = {4819--4829},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Interplay of Interactions Governing the Dynamic Conversions of Acyclic and Macrocyclic Helicates Article de journal Victoria E. Campbell; Xavier de Hatten; Nicolas Delsuc; Brice Kauffmann; Ivan Huc; Jonathan R. Nitschke Chemistry - A European Journal, 15 (25), p. 6138–6142, 2009, ISSN: 09476539, 15213765. @article{campbell_interplay_2009,
title = {Interplay of Interactions Governing the Dynamic Conversions of Acyclic and Macrocyclic Helicates},
author = {Victoria E. Campbell and Xavier de Hatten and Nicolas Delsuc and Brice Kauffmann and Ivan Huc and Jonathan R. Nitschke},
url = {http://doi.wiley.com/10.1002/chem.200900693},
doi = {10.1002/chem.200900693},
issn = {09476539, 15213765},
year = {2009},
date = {2009-01-01},
urldate = {2018-02-18},
journal = {Chemistry - A European Journal},
volume = {15},
number = {25},
pages = {6138--6142},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2008
|
Kinetics of Helix-Handedness Inversion: Folding and Unfolding in Aromatic Amide Oligomers Article de journal Nicolas Delsuc; Takahiro Kawanami; Julien Lefeuvre; Atsuomi Shundo; Hirotaka Ihara; Makoto Takafuji; Ivan Huc ChemPhysChem, 9 (13), p. 1882–1890, 2008, ISSN: 14394235, 14397641. @article{delsuc_kinetics_2008,
title = {Kinetics of Helix-Handedness Inversion: Folding and Unfolding in Aromatic Amide Oligomers},
author = {Nicolas Delsuc and Takahiro Kawanami and Julien Lefeuvre and Atsuomi Shundo and Hirotaka Ihara and Makoto Takafuji and Ivan Huc},
url = {http://doi.wiley.com/10.1002/cphc.200800310},
doi = {10.1002/cphc.200800310},
issn = {14394235, 14397641},
year = {2008},
date = {2008-01-01},
urldate = {2018-02-18},
journal = {ChemPhysChem},
volume = {9},
number = {13},
pages = {1882--1890},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Metal-Directed Dynamic Formation of Tertiary Structure in Foldamer Assemblies: Orienting Helices at an Angle Article de journal Nicolas Delsuc; Marie Hutin; Victoria E. Campbell; Brice Kauffmann; Jonathan R. Nitschke; Ivan Huc Chemistry - A European Journal, 14 (24), p. 7140–7143, 2008, ISSN: 09476539, 15213765. @article{delsuc_metal-directed_2008,
title = {Metal-Directed Dynamic Formation of Tertiary Structure in Foldamer Assemblies: Orienting Helices at an Angle},
author = {Nicolas Delsuc and Marie Hutin and Victoria E. Campbell and Brice Kauffmann and Jonathan R. Nitschke and Ivan Huc},
url = {http://doi.wiley.com/10.1002/chem.200800988},
doi = {10.1002/chem.200800988},
issn = {09476539, 15213765},
year = {2008},
date = {2008-01-01},
urldate = {2018-02-18},
journal = {Chemistry - A European Journal},
volume = {14},
number = {24},
pages = {7140--7143},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2007
|
The Herringbone Helix: A Noncanonical Folding in Aromatic−Aliphatic Peptides Article de journal Nicolas Delsuc; Frédéric Godde; Brice Kauffmann; Jean-Michel Léger; Ivan Huc Journal of the American Chemical Society, 129 (37), p. 11348–11349, 2007, ISSN: 0002-7863, 1520-5126. @article{delsuc_herringbone_2007,
title = {The Herringbone Helix: A Noncanonical Folding in Aromatic−Aliphatic Peptides},
author = {Nicolas Delsuc and Fr\'{e}d\'{e}ric Godde and Brice Kauffmann and Jean-Michel L\'{e}ger and Ivan Huc},
url = {http://pubs.acs.org/doi/abs/10.1021/ja074285s},
doi = {10.1021/ja074285s},
issn = {0002-7863, 1520-5126},
year = {2007},
date = {2007-01-01},
urldate = {2018-02-18},
journal = {Journal of the American Chemical Society},
volume = {129},
number = {37},
pages = {11348--11349},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Proteomorphous Objects from Abiotic Backbones Article de journal Nicolas Delsuc; Jean-Michel Léger; Stéphane Massip; Ivan Huc Angewandte Chemie International Edition, 46 (1-2), p. 214–217, 2007, ISSN: 14337851, 15213773. @article{delsuc_proteomorphous_2007,
title = {Proteomorphous Objects from Abiotic Backbones},
author = {Nicolas Delsuc and Jean-Michel L\'{e}ger and St\'{e}phane Massip and Ivan Huc},
url = {http://doi.wiley.com/10.1002/anie.200603390},
doi = {10.1002/anie.200603390},
issn = {14337851, 15213773},
year = {2007},
date = {2007-01-01},
urldate = {2018-02-18},
journal = {Angewandte Chemie International Edition},
volume = {46},
number = {1-2},
pages = {214--217},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2006
|
Vibrational circular dichroism and ab initio structure elucidation of an aromatic foldamer Article de journal Thierry Buffeteau; Laurent Ducasse; Legiso Poniman; Nicolas Delsuc; Ivan Huc Chemical Communications, (25), p. 2714, 2006, ISSN: 1359-7345, 1364-548X. @article{buffeteau_vibrational_2006,
title = {Vibrational circular dichroism and ab initio structure elucidation of an aromatic foldamer},
author = {Thierry Buffeteau and Laurent Ducasse and Legiso Poniman and Nicolas Delsuc and Ivan Huc},
url = {http://xlink.rsc.org/?DOI=b604462j},
doi = {10.1039/b604462j},
issn = {1359-7345, 1364-548X},
year = {2006},
date = {2006-01-01},
urldate = {2018-02-18},
journal = {Chemical Communications},
number = {25},
pages = {2714},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2005
|
Probing helix propensity of monomers within a helical oligomer Article de journal Christel Dolain; Jean-Michel Léger; Nicolas Delsuc; Heinz Gornitzka; Ivan Huc Proceedings of the National Academy of Sciences of the United States of America, 102 (45), p. 16146–16151, 2005. @article{dolain_probing_2005,
title = {Probing helix propensity of monomers within a helical oligomer},
author = {Christel Dolain and Jean-Michel L\'{e}ger and Nicolas Delsuc and Heinz Gornitzka and Ivan Huc},
year = {2005},
date = {2005-01-01},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
volume = {102},
number = {45},
pages = {16146--16151},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
0000
|
A di-Copper Peptidyl Complex Mimics the Activity of Catalase, a Key Antioxidant Metalloenzyme Article de journal Koudedja Coulibaly; Marion Thauvin; Adyn Melenbacher; Clara Testard; Evangelia Trigoni; Amandine Vincent; Martin J Stillman; Sophie Vriz; Clotilde Policar; Nicolas Delsuc Inorganic Chemistry, 0 (0), p. null, 0000, (PMID: 34109781). @article{doi:10.1021/acs.inorgchem.0c03718,
title = {A di-Copper Peptidyl Complex Mimics the Activity of Catalase, a Key Antioxidant Metalloenzyme},
author = {Koudedja Coulibaly and Marion Thauvin and Adyn Melenbacher and Clara Testard and Evangelia Trigoni and Amandine Vincent and Martin J Stillman and Sophie Vriz and Clotilde Policar and Nicolas Delsuc},
url = {https://doi.org/10.1021/acs.inorgchem.0c03718},
doi = {10.1021/acs.inorgchem.0c03718},
year = {0000},
date = {0000-01-01},
journal = {Inorganic Chemistry},
volume = {0},
number = {0},
pages = {null},
abstract = {Catalases (CAT) are antioxidant metalloenzymes necessary for life in oxygen-metabolizing cells to regulate H2O2 concentration by accelerating its dismutation. Many physiopathological situations are associated with oxidative stress resulting from H2O2 overproduction, during which antioxidant defenses are overwhelmed. We have used a combinatorial approach associated with an activity-based screening to discover a first peptidyl di-copper complex mimicking CAT. The complex was studied in detail and characterized for its CAT activity both in solutions and in cells using different analytical methods. The complex exhibited CAT activity in solutions and, more interestingly, on HyPer HeLa cells that possess a genetically encoded ratiometric fluorescent sensors of H2O2. These results highlight the efficiency of a combinatorial approach for the discovery of peptidyl complexes that exhibit catalytic activity.},
note = {PMID: 34109781},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Catalases (CAT) are antioxidant metalloenzymes necessary for life in oxygen-metabolizing cells to regulate H2O2 concentration by accelerating its dismutation. Many physiopathological situations are associated with oxidative stress resulting from H2O2 overproduction, during which antioxidant defenses are overwhelmed. We have used a combinatorial approach associated with an activity-based screening to discover a first peptidyl di-copper complex mimicking CAT. The complex was studied in detail and characterized for its CAT activity both in solutions and in cells using different analytical methods. The complex exhibited CAT activity in solutions and, more interestingly, on HyPer HeLa cells that possess a genetically encoded ratiometric fluorescent sensors of H2O2. These results highlight the efficiency of a combinatorial approach for the discovery of peptidyl complexes that exhibit catalytic activity. |
