Clotilde POLICAR

Professor, Ecole Normale Supérieure, PSL université Paris
Dean of the studies at the department of chemistry of ENS
Deputy director of the Laboratoire des BioMolécules (LBM), UMR7203, CNRS-PSL-SU
Scientific deputy director of the Institute of chemistry at CNRS (DAS-INC-CNRS), in charge of interdisciplinarity

ENS – Département de chimie
24 rue Lhomond, 75005 Paris, France

Email: clotilde.policar@ens.psl.eu
Phone: +33 144322424
Office: E037

Short bio

Clotilde Policar was trained in organic, inorganic and physical chemistry and she then specialized in bio-inorganic chemistry. After a Ph.D. under the supervision of Dr. Daniel Mansuy and Dr. Isabelle Artaud at Paris-Sud 11 and Paris 5 universities on mimics of a metalloprotein (manganese peroxidase), she worked as a post-doctoral fellow on high-field electron paramagnetic resonance to characterize complexes mimicking the Mn cluster from the oxygen evolving center in the photosystem II with Dr. Sun Un and Dr. William Rutherford. She was then appointed assistant professor at Paris-Sud 11, where she dedicated her work to the development of Mn-complexes reproducing the activity of superoxide-dismutase (SOD). The initial focus was the spectroscopic characterization of Mn-OO adducts involved in the catalytic cycle for superoxide dismutation. She became an associate professor in 2005. Because the metalloproteins SODs are involved in the cellular protection against oxidative stress, the next step she took was to study these compounds in cellular models of oxidative stress. Since 2008, she has a professorship at the Ecole Normale Supérieure—Paris Sciences et Lettres, where she has set up a thematic group in bioinorganic chemistry or more precisely in inorganic biological and cellular chemistry. This emerging thematic is dedicated to the direct study of inorganic compounds in biological environments. Her main current interests focus, on the one hand, on the design of manganese-based anti-oxidants and their evaluation in cells. On the other, her group has initiated a new topic with the development of metal-based probes, including metal–carbonyl as multimodal bioprobes and that they validated as efficient IR-probe for bio-imaging.

Education and professional experience

  • Short CV
  • Detailed CV
  • Since 2019 (Sept)  Responsible of the two-year master programme in Chemistry and Life Science (parcours CSDV du master de chimie de Paris Centre)
    Since 2017              Member of the editorial board of Journal of Inorganic Biochemistry
    Since 2016              Deputy director of the Institut de Chimie du CNRS in charge of interdisciplinarity                                     (Directrice adjointe science DAS-INC)
    2015-2017              Member of the advisory board of Inorganic Chemistry (ACS)
    Since 2015              Deputy chair of the FrenchBIC   (French network in BioInorganic Chemistry, network of 25 laboratories)
    Since 2014 (Sept)  Dean of the studies, co-responsible for the international relationships
    Since 2014 (Jan)    Deputy director of the Research Unit LBM
    2008                         Nomination as a professor at the ENS
    2005-2008             Associate professor, Paris-Sud 11 university
    2005 (nov.)             Habilitation thesis, Paris-Sud 11 university
    1996-2005             Assistant professor, Paris-Sud 11 university
    Institut de Chimie Moléculaire et des Matériaux d’Orsay

Research interests

  • Bioinorganic chemistry
  • Superoxide dismutase mimics, superoxide, cellular models of oxidative stress, metal-based anti-oxidants
  • Metal-carbonyls as multimodal probes for imaging: IR-spectromicroscopy, micro X-ray fluorescence and classical fluorescence
  • Metals in biology: intracellular detection,  metal imaging

Awards, distinctions, scientific societies membership, and contracts

  • Elected member of the international council of the SBIC in October 2019.
  • Member of the Société chimique de France and of the FrenchBIC
  • Research contracts since 2008:
    -Research contract from FRM as PI (Fédération pour la recherche médicale, Appels Pionniers de la recherche Etudes Physico-chimiques innovantes pour la biologie et la médecine- 2016-2020)
    -Research fellowship from the Association François Aupetit (inflammatory bowel disease patients) obtained in 2015
    -ANR contracts (National Funding Agency) : one as a PI (MAGIC, 2015-2020) ; three ANR contracts as a partner (Take Care, PI M. Robert, 2011-13 ; Metabact, PI I. Artaud, 2011-13, and MnHFPELDOR, PI L. Tabares, 2012-2015,)
    -Paris-Sciences-Lettres research contract (PI, 2014-15)
    -CNRS-contract Interdisciplinary work (interface chemistry-physic-biology), support for risky research (2009)
    -Young researcher grant (ACI jeune chercheur) (2005-08)
    -Since 2008: more than 10 projects selected on synchrotron beamlines (SOLEIL, APS, Alladin).
  • 1997: first prize “Creation” for the French Ministry of Research for the project Le ticket d’Archimède (posters of popularisation of science in the Parisian Metro), project directed by C. Policar from 1995 to 1998.

Supervised students and post-doctorants

  • Formers (not available)
  • Currents:
    -Martha Zoumpoulaki, PhD since October 2017
    -Koudedja Coulibaly, PhD since October 2017
    -Antoine Hoste, PhD since October 2017

Teaching

  • Bioinorganic chemistry: M1-ENS and Sorbonne université;  master de chimie de Paris Centre, parcours chemistry and life science
  • Metals in biology: M2-master de chimie de Paris Centre, parcours chimie moléculaire
  • History of Sciences: organisation of the annual conference day « Journée Bram »

Significant publications

Graftable SCoMPIs enable the labeling and X-Ray fluorescence imaging of proteins, Sarah Hostachy, Marie Masuda, Takayuki Miki, Itaru Hamachi, Sandrine Sagan, Olivier Lequin, Kadda Medjoubi, Andrea Somogyi, Nicolas Delsuc and Clotilde Policar*, Chem. Sci. 2018 9, 4483-4487, doi 10.1039/C8SC00886H

A cell penetrant manganese SOD-mimic is able to complement MnSOD and exerts an anti-inflammatory effect on cellular and animal models of inflammatory bowel diseases, Emilie Mathieu, Anne-Sophie Bernard, Nicolas Delsuc, Elodie Quévrain, Géraldine Gazzah, Barry Lai, Florian Chain, Philippe Langella, Maria Bachelet, Joelle Masliah, Philippe Seksik, and Clotilde Policar*, Inorg Chem. 2017, 56, 2545-2555, IF 4.82 (2016), doi: dx.doi.org/10.1021/acs.inorgchem.6b02695

Entasis Through Hook-and-Loop Fastening in a Glycoligand with Cumulative Weak Forces Stabilizing CuI, Ludivine Garcia, Federico Cisnetti, Natacha Gillet, Régis Guillot, Magali Aumont-Niciase, Jean-Philip Piquemal, Michel Desmadril, François Lambert, Clotilde Policar*, J. Am. Chem. Soc., 2015, 137, 1141−1146, DOI: 10.1021/ja510259p,

A Rhenium tris-Carbonyl Derivative as a Single Core Multimodal Probe for Imaging (SCoMPI) Combining Infrared and Luminescent Properties, Sylvain Clède, François Lambert, Christophe Sandt, Zoher Gueroui, Matthieu Réfrégiers, Marie-Aude Plamont, Paul Dumas, Anne Vessières, Clotilde Policar*, Chem. Commun., 2012, 48, 7729–7731, doi : 10.1039/C2CC32163G,

Subcellular Imaging in the Mid-IR of a Metal-Carbonyl Moiety using Photothermal Induced Resonance, Clotilde Policar*, Jenny-Birgitta Waern, Marie-Aude Plamont, Sylvain Clède, Céline Mayet, Rui Prazeres, Jean-Michel Ortega, Anne Vessières, Alexandre Dazzi, Angew. Chem., Int. Ed., 2011, 50, 860-864; doi : 10.1002/anie.201003161, Highlighted with an inside cover.

Publications

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2014

Luminescence modulations of rhenium tricarbonyl complexes induced by structural variations Article de journal

H C Bertrand; S Clède; R Guillot; F Lambert; C Policar

Inorganic Chemistry, 53 (12), p. 6204–6223, 2014.

Résumé | Liens

Fourier transform infrared (FT-IR) spectromicroscopy to identify cell organelles: Correlation with fluorescence staining in MCF-7 breast cancer cells Article de journal

S Clède; C Policar; C Sandt

Applied Spectroscopy, 68 (1), p. 113–117, 2014.

Résumé | Liens

From IR-Spectromicroscopy using AFM-IR and SR-FTIR to Bimodal Spectromicroscopy using SCoMPIs - Single Core Multimodal Probe for Imaging Article de journal

C Policar; C Sylvain; F Lambert; N Delsuc; C Sandt; P Dumas; M Refregiers; M Plamont; A Vessieres; Z Gueroui; A Dazzi

Journal of Biological Inorganic Chemistry, 19 , p. S182-S182, 2014, ISSN: 0949-8257.

Liens

2013

Toward optimal spatial and spectral quality in widefield infrared spectromicroscopy of IR labelled single cells Article de journal

E C Mattson; M Unger; S Clède; F Lambert; C Policar; A Imtiaz; R D'Souza; C J Hirschmugl

Analyst, 138 (19), p. 5610–5618, 2013.

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Polypyrrole functionalized with new copper complex as platform for His-tag antibody immobilization and direct antigen detection Article de journal

S Chebil; A Miodek; V Ambike; H Sauriat-Dorizon; C Policar; H Korri-Youssoufi

Sensors and Actuators, B: Chemical, 185 , p. 762–770, 2013.

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Synchrotron radiation FTIR detection of a metal-carbonyl tamoxifen analog. Correlation with luminescence microscopy to study its subcellular distribution Article de journal

S Clède; F Lambert; C Sandt; Z Gueroui; N Delsuc; P Dumas; A Vessières; C Policar

Biotechnology Advances, 31 (3), p. 393–395, 2013.

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An intrinsically fluorescent glycoligand for direct imaging of ligand trafficking in artificial and living cell systems Article de journal

L Garcia; M Lazzaretti; A Diguet; F Mussi; F Bisceglie; J Xie; G Pelosi; A Buschini; D Baigl; C Policar

New Journal of Chemistry, 37 (10), p. 3030–3034, 2013.

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Detection of an estrogen derivative in two breast cancer cell lines using a single core multimodal probe for imaging (SCoMPI) imaged by a panel of luminescent and vibrational techniques Article de journal

S Clède; F Lambert; C Sandt; S Kascakova; M Unger; E Harté; M -A Plamont; R Saint-Fort; A Deniset-Besseau; Z Gueroui; C Hirschmugl; S Lecomte; A Dazzi; A Vessières; C Policar

Analyst, 138 (19), p. 5627–5638, 2013.

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2012

A rhenium tris-carbonyl derivative as a single core multimodal probe for imaging (SCoMPI) combining infrared and luminescent properties Article de journal

S Clède; F Lambert; C Sandt; Z Gueroui; M Réfrégiers; M -A Plamont; P Dumas; A Vessières; C Policar

Chemical Communications, 48 (62), p. 7729–7731, 2012.

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Evaluation of the anti-oxidant properties of a SOD-mimic Mn-complex in activated macrophages Article de journal

A -S Bernard; C Giroud; H Y V Ching; A Meunier; V Ambike; C Amatore; M G Collignon; F Lemaître; C Policar

Dalton Transactions, 41 (21), p. 6399–6403, 2012.

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Glycosiderophores: Synthesis of tris-hydroxamate siderophores based on a galactose or glycero central scaffold, Fe(III) complexation studies Article de journal

C Neff; F Bellot; J -B Waern; F Lambert; J Brandel; G Serratrice; F Gaboriau; C Policar

Journal of Inorganic Biochemistry, 112 , p. 59–67, 2012.

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Recent analytical applications of molecular spectroscopy in bioorganometallic chemistrypart I: Metal carbonyls Article de journal

I S Butler; R P Kengne-Momo; G Jaouen; C Policar; A Vessières

Applied Spectroscopy Reviews, 47 (7), p. 531–549, 2012.

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Recent applications of molecular spectroscopy in bioorganometallic chemistry-Part 2: Ferrocenes and other organometallic complexes Article de journal

I S Butler; R P Kengne-Momo; A Vessières; G Jaouen; C Policar

Applied Spectroscopy Reviews, 47 (8), p. 620–632, 2012.

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Metal complexation of a Đ -ribose-based ligand decoded by experimental and theoretical studies Article de journal

F Cisnetti; J -D Maréchal; M Nicaise; R Guillot; M Desmadril; F Lambert; C Policar

European Journal of Inorganic Chemistry, (20), p. 3308–3319, 2012.

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2011

Subcellular IR imaging of a metal-carbonyl moiety using photothermally induced resonance Article de journal

C Policar; J B Waern; M -A Plamont; S Clède; C Mayet; R Prazeres; J -M Ortega; A Vessières; A Dazzi

Angewandte Chemie - International Edition, 50 (4), p. 860–864, 2011.

Résumé | Liens

Proton-coupled electron transfers in biomimetic water bound metal complexes. the electrochemical approach Article de journal

E Anxolabéhère-Mallart; C Costentin; C Policar; M Robert; J -M Savéant; A -L Teillout

Faraday Discussions, 148 , p. 83–95, 2011.

Résumé | Liens

Intrinsically fluorescent glycoligands to study metal selectivity Article de journal

L Garcia; S Maisonneuve; J Oudinet-Sin Marcu; R Guillot; F Lambert; J Xie; C Policar

Inorganic Chemistry, 50 (22), p. 11353–11362, 2011.

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2010

Sugars to control ligand shape in metal complexes: Conformationally constrained glycoligands with a predetermination of stereochemistry and a structural control Article de journal

L Garcia; S Maisonneuve; J Xie; R Guillot; P Dorlet; E Rivière; M Desmadril; F Lambert; C Policar

Inorganic Chemistry, 49 (16), p. 7282–7288, 2010.

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2009

Design, self-assembly, and molecular structures of 3D copper(II) capsules templated by BF4 - guest anions Article de journal

C Desmarets; C Policar; L -M Chamoreau; H Amouri

European Journal of Inorganic Chemistry, (29-30), p. 4396–4400, 2009.

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