Clotilde POLICAR

Professor, Ecole Normale Supérieure, PSL university
Dean of the sciences studies of Ecole Normale Supérieure
Deputy director of the Laboratoire des BioMolécules (LBM), UMR7203, CNRS-ENS-PSL-SU

ENS-PSL
Département de chimie: 24, rue Lhomond, 75005 Paris, France
Direction des études sciences: 45, rue d’Ulm, 75005 Paris, France

Email: clotilde.policar@ens.psl.eu
Phone: +33 144322424
Office: E037

Website of the research group « metals in biology »: https://www.chimie.ens.fr/bic/ and https://www.chimie.ens.fr/recherche/laboratoire-lbm/peptides-glycoconjugues-metaux-biologie/bioinorganic-chemistry/

Short bio

Clotilde Policar was trained in organic, inorganic and physical chemistry, after which she specialized in bio-inorganic chemistry. After completing Ph.D. under the supervision of Dr. Daniel Mansuy and Dr. Isabelle Artaud at Paris-Sud 11 and Paris 5 universities on mimics of a metalloprotein (manganese peroxidase), she worked as a post-doctoral fellow on high-field electron paramagnetic resonance to characterize complexes a spectroscopic mimics of the Mn cluster from the oxygen evolving center in the photosystem II with Dr. Sun Un and Dr. William Rutherford. She was then appointed an assistant professor at Paris-Sud 11 (now Paris Saclay university), where she dedicated her work to the development of Mn-complexes reproducing the activity of superoxide-dismutase (SOD). The initial focus was the spectroscopic characterization of Mn-OO adducts involved in the catalytic cycle for superoxide dismutation. She became an associate professor in 2005. Because the metalloproteins SODs are involved in the cellular protection against oxidative stress, the next step she took was to study these compounds in cellular models of oxidative stress. Since 2008, she has a professorship at the Ecole Normale Supérieure—Paris Sciences et Lettres, where she has set up a thematic group in bioinorganic chemistry or more precisely in inorganic biological and cellular chemistry. This emerging thematic is dedicated to the direct study of inorganic compounds in biological environments. Her main current interests focus, on the one hand, on the design of manganese-based anti-oxidants and their evaluation in cells. On the other hand, her group has initiated a new topic with the development of metal-based probes, including metal–carbonyl as multimodal bioprobes and that they validated as efficient multimodal IR, fluorescence and X-fluorescence for bio-imaging.

Education and professional experience

  • Short CV and detailed publication list: https://orcid.org/0000-0003-0255-1650
  • Since 2020: dean of the science education at the Ecole normale supérieure
    2019-2020: responsible of the two-year master programme in Chemistry and Life Science (parcours Chimie et sciences du vivant du master de chimie de Paris Centre)
    Since 2019: elected member of the SBIC council (https://www.sbichem.org/)
    2017-2021: member of the editorial board of Journal of Inorganic Biochemistry
    2016-2020: deputy director of the Institut de Chimie du CNRS in charge of interdisciplinarity, work in the MITI-CNRS (mission pour les initiatives transverses et l’interdisciplinarité)
    2015-2017: member of the advisory board of Inorganic Chemistry (ACS)
    2015-2020: deputy chair of the FrenchBIC   (French network in BioInorganic Chemistry, network of 25 laboratories)
    2014-2020:  dean of the studies at the department of chemistry of ENS, co-responsible for the international relationships
    Since 2014:  deputy director of the Research Unit LBM
    2008: nomination as a professor at the ENS
    2005-2008: associate professor, Paris-Sud 11 university (now Paris Saclay)
    2005 (nov.): habilitation thesis, Paris-Sud 11 university
    1996-2005: assistant professor, Paris-Sud 11 university, Institut de Chimie Moléculaire et des Matériaux d’Orsay (ICMMO)

Research interests

    • Bioinorganic chemistry
    • Superoxide dismutase mimics, superoxide, cellular models of oxidative stress, metal-based anti-oxidants
    • Metal-carbonyles as multimodal probes for imaging: IR-spectromicroscopy, micro X-ray fluorescence and classical fluorescence
    • Metals in biology: intracellular detection,  metal imaging
    • Oxidative stress

About a recent project funded by ANR (nation research agency): https://www.ens.psl.eu/actualites/des-catalyseurs-bio-inspires-pour-lutter-contre-le-stress-oxydant

Awards, distinctions, scientific societies membership, and contracts

  • Elected member of the international council of the SBIC in October 2019.
  • Member of the Société chimique de France and of the FrenchBIC
  • Research contracts since 2008:
    -ANR contracts (National Funding Agency) : two as a PI (CATMAN, 2020-2024 ; MAGIC, 2015-2021) ; four ANR contracts as a partner (MIBIDIC, PI P. Seksikk (2022-2026) ; MnHFPELDOR, PI L. Tabares, 2012-2015, Take Care, PI M. Robert, 2011-13 ; Metabact, PI I. Artaud, 2011-13)
    -Qlife initiative: project MAIN (2021-2022) as PI (Mn-Based Antioxidant evaluation in INflammatory Epithelial Cells)
    -Research contract from FRM as PI (Fédération pour la recherche médicale, Appels Pionniers de la recherche Etudes Physico-chimiques innovantes pour la biologie et la médecine- 2016-2020)
    -Research fellowship from the Association François Aupetit (inflammatory bowel disease patients) obtained in 2015
    -Paris-Sciences-Lettres research contract (PI, 2014-15)
    -CNRS-contract Interdisciplinary work (interface chemistry-physic-biology), support for risky research (2009)
    -Young researcher grant (ACI jeune chercheur) (2005-08)
    -Since 2008: more than 15 projects selected on synchrotron beamlines (SOLEIL, APS, Aladdin).
  • 1997: first prize “Creation” for the French Ministry of Research for the project Le ticket d’Archimède (posters of popularisation of science in the Parisian Metro), project directed by C. Policar from 1995 to 1998.

Supervised students and post-doctorants

  • Formers (not yet available)
  • Current:
    -Jean Bouvet, PhD since October 2020, co-supervized with Prof. S. Demignot, IPV fellowship
    -Martha Zoumpoulaki, PhD since October 2017, ANR fellowship
    -Antoine Hoste, PhD since October 2017, ENS fellowship

Teaching

  • Bioinorganic chemistry: M1-ENS and Sorbonne université;  master de chimie de Paris Centre, parcours chemistry and life science
  • Metals in biology: M2-master de chimie de Paris Centre, parcours chimie moléculaire
  • History of Sciences: organisation of the annual conference day « Journée Bram »

Five significant publications

Intracellular location matters: rationalization of the anti-inflammatory activity of a manganese(II) superoxide dismutase mimic complex, Emilie Mathieu, Anne-Sophie Bernard, Elodie Quévrain, Martha Zoumpoulaki, Sébastien Iriart, Caroline Lung-Soong, Barry Lai, Kadda Medjoubi, Lucas Henry, Sounderya Nagarajan, Florent Poyer, Andrea Scheitler, Ivana Ivanović-Burmazović, Sergio Marco, Andrea Somogyi, Philippe Seksik, Nicolas Delsuc, Clotilde Policar, Chem. Commun., 2020, 56, 7885 – 7888, https://doi.org/10.1039/D0CC03398G

Graftable SCoMPIs enable the labeling and X-Ray fluorescence imaging of proteins, Sarah Hostachy, Marie Masuda, Takayuki Miki, Itaru Hamachi, Sandrine Sagan, Olivier Lequin, Kadda Medjoubi, Andrea Somogyi, Nicolas Delsuc and Clotilde Policar*, Chem. Sci. 2018 9, 4483-4487, doi 10.1039/C8SC00886H

A cell penetrant manganese SOD-mimic is able to complement MnSOD and exerts an anti-inflammatory effect on cellular and animal models of inflammatory bowel diseases, Emilie Mathieu, Anne-Sophie Bernard, Nicolas Delsuc, Elodie Quévrain, Géraldine Gazzah, Barry Lai, Florian Chain, Philippe Langella, Maria Bachelet, Joelle Masliah, Philippe Seksik, and Clotilde Policar*, Inorg Chem. 2017, 56, 2545-2555, IF 4.82 (2016), doi: dx.doi.org/10.1021/acs.inorgchem.6b02695

Entasis Through Hook-and-Loop Fastening in a Glycoligand with Cumulative Weak Forces Stabilizing CuI, Ludivine Garcia, Federico Cisnetti, Natacha Gillet, Régis Guillot, Magali Aumont-Niciase, Jean-Philip Piquemal, Michel Desmadril, François Lambert, Clotilde Policar*, J. Am. Chem. Soc., 2015, 137, 1141−1146, DOI: 10.1021/ja510259p,

Subcellular Imaging in the Mid-IR of a Metal-Carbonyl Moiety using Photothermal Induced Resonance, Clotilde Policar*, Jenny-Birgitta Waern, Marie-Aude Plamont, Sylvain Clède, Céline Mayet, Rui Prazeres, Jean-Michel Ortega, Anne Vessières, Alexandre Dazzi, Angew. Chem., Int. Ed., 2011, 50, 860-864; doi : 10.1002/anie.201003161, Highlighted with an inside cover.

Publications

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2015

An easy-to-detect nona-arginine peptide for epidermal targeting Article de journal

S Clède; N Delsuc; C Laugel; F Lambert; C Sandt; A Baillet-Guffroy; C Policar

Chemical Communications, 51 (13), p. 2687–2689, 2015.

Fast magnetically driven electrodeposition of amorphous metal oxide water oxidation catalysts from carbon-coated metallic nanoparticles Article de journal

J Zhu; F Lambert; C Policar; F Mavré; B Limoges

Journal of Materials Chemistry A, 3 (31), p. 16190–16197, 2015.

Entasis through Hook-and-Loop fastening in a glycoligand with cumulative weak forces stabilizing CuI Article de journal

L Garcia; F Cisnetti; N Gillet; R Guillot; M Aumont-Nicaise; J -P Piquemal; M Desmadril; F Lambert; C Policar

Journal of the American Chemical Society, 137 (3), p. 1141–1146, 2015.

2014

Influence of the side-chain length on the cellular uptake and the cytotoxicity of rhenium triscarbonyl derivatives: A bimodal infrared and luminescence quantitative study Article de journal

S Clède; F Lambert; R Saint-Fort; M -A Plamont; H Bertrand; A Vessières; C Policar

Chemistry - A European Journal, 20 (28), p. 8714–8722, 2014.

Electrochemical formation and reactivity of a manganese peroxo complex: Acid driven Ħ2O2 generation vs. O-O bond cleavage Article de journal

H Y V Ching; E Anxolabéhère-Mallart; H E Colmer; C Costentin; P Dorlet; T A Jackson; C Policar; M Robert

Chemical Science, 5 (6), p. 2304–2310, 2014.

Conjugation of a new series of dithiocarbazate schiff base copper(II) complexes with vectors selected to enhance antibacterial activity Article de journal

M L Low; L Maigre; P Dorlet; R Guillot; J -M Pagès; K A Crouse; C Policar; N Delsuc

Bioconjugate Chemistry, 25 (12), p. 2269–2284, 2014.

Apo-neocarzinostatin: A protein carrier for Cu(II) glycocomplexes and Cu(II) into U937 and HT29 cell lines Article de journal

L Garcia; S Franzoni; F Mussi; M Aumont-Niçaise; H Bertrand; M Desmadril; G Pelosi; A Buschini; C Policar

Journal of Inorganic Biochemistry, 135 , p. 40–44, 2014.

Luminescence modulations of rhenium tricarbonyl complexes induced by structural variations Article de journal

H C Bertrand; S Clède; R Guillot; F Lambert; C Policar

Inorganic Chemistry, 53 (12), p. 6204–6223, 2014.

Fourier transform infrared (FT-IR) spectromicroscopy to identify cell organelles: Correlation with fluorescence staining in MCF-7 breast cancer cells Article de journal

S Clède; C Policar; C Sandt

Applied Spectroscopy, 68 (1), p. 113–117, 2014.

From IR-Spectromicroscopy using AFM-IR and SR-FTIR to Bimodal Spectromicroscopy using SCoMPIs - Single Core Multimodal Probe for Imaging Article de journal

C Policar; C Sylvain; F Lambert; N Delsuc; C Sandt; P Dumas; M Refregiers; M Plamont; A Vessieres; Z Gueroui; A Dazzi

Journal of Biological Inorganic Chemistry, 19 , p. S182-S182, 2014, ISSN: 0949-8257.

Anti-oxidant Mn-complexes: evaluation in cellular models of oxidative stress Article de journal

Clotilde Policar; Anne-Sophie Bernard; Nicolas Delsuc; Geraldine Gazzah; Manon Guille; Frederic Lemaitre; Christian Amatore; Maria Bachelet; Joelle Masliah

Journal of Biological Inorganic Chemistry, 19 , p. S739-S740, 2014, (Times Cited: 0 2 12th European Biological Inorganic Chemistry Conference (EuroBIC) Aug 24-28, 2014 Zurich, SWITZERLAND Univ Zurich).

2013

Toward optimal spatial and spectral quality in widefield infrared spectromicroscopy of IR labelled single cells Article de journal

E C Mattson; M Unger; S Clède; F Lambert; C Policar; A Imtiaz; R D'Souza; C J Hirschmugl

Analyst, 138 (19), p. 5610–5618, 2013.

Polypyrrole functionalized with new copper complex as platform for His-tag antibody immobilization and direct antigen detection Article de journal

S Chebil; A Miodek; V Ambike; H Sauriat-Dorizon; C Policar; H Korri-Youssoufi

Sensors and Actuators, B: Chemical, 185 , p. 762–770, 2013.

Synchrotron radiation FTIR detection of a metal-carbonyl tamoxifen analog. Correlation with luminescence microscopy to study its subcellular distribution Article de journal

S Clède; F Lambert; C Sandt; Z Gueroui; N Delsuc; P Dumas; A Vessières; C Policar

Biotechnology Advances, 31 (3), p. 393–395, 2013.

An intrinsically fluorescent glycoligand for direct imaging of ligand trafficking in artificial and living cell systems Article de journal

L Garcia; M Lazzaretti; A Diguet; F Mussi; F Bisceglie; J Xie; G Pelosi; A Buschini; D Baigl; C Policar

New Journal of Chemistry, 37 (10), p. 3030–3034, 2013.

Detection of an estrogen derivative in two breast cancer cell lines using a single core multimodal probe for imaging (SCoMPI) imaged by a panel of luminescent and vibrational techniques Article de journal

S Clède; F Lambert; C Sandt; S Kascakova; M Unger; E Harté; M -A Plamont; R Saint-Fort; A Deniset-Besseau; Z Gueroui; C Hirschmugl; S Lecomte; A Dazzi; A Vessières; C Policar

Analyst, 138 (19), p. 5627–5638, 2013.

2012

A rhenium tris-carbonyl derivative as a single core multimodal probe for imaging (SCoMPI) combining infrared and luminescent properties Article de journal

S Clède; F Lambert; C Sandt; Z Gueroui; M Réfrégiers; M -A Plamont; P Dumas; A Vessières; C Policar

Chemical Communications, 48 (62), p. 7729–7731, 2012.

Glycosiderophores: Synthesis of tris-hydroxamate siderophores based on a galactose or glycero central scaffold, Fe(III) complexation studies Article de journal

C Neff; F Bellot; J -B Waern; F Lambert; J Brandel; G Serratrice; F Gaboriau; C Policar

Journal of Inorganic Biochemistry, 112 , p. 59–67, 2012.

Recent analytical applications of molecular spectroscopy in bioorganometallic chemistrypart I: Metal carbonyls Article de journal

I S Butler; R P Kengne-Momo; G Jaouen; C Policar; A Vessières

Applied Spectroscopy Reviews, 47 (7), p. 531–549, 2012.

Recent applications of molecular spectroscopy in bioorganometallic chemistry-Part 2: Ferrocenes and other organometallic complexes Article de journal

I S Butler; R P Kengne-Momo; A Vessières; G Jaouen; C Policar

Applied Spectroscopy Reviews, 47 (8), p. 620–632, 2012.

Metal complexation of a Đ -ribose-based ligand decoded by experimental and theoretical studies Article de journal

F Cisnetti; J -D Maréchal; M Nicaise; R Guillot; M Desmadril; F Lambert; C Policar

European Journal of Inorganic Chemistry, (20), p. 3308–3319, 2012.

Evaluation of the anti-oxidant properties of a SOD-mimic Mn-complex in activated macrophages Article de journal

Anne-Sophie Bernard; Claire Giroud; Vincent H Y Ching; Anne Meunier; Vinita Ambike; Christian Amatore; Manon Guille-Collignon; Frederic Lemaitre; Clotilde Policar

Dalton Transactions, 41 (21), p. 6399-6403, 2012, (Times Cited: 23).

2011

Subcellular IR imaging of a metal-carbonyl moiety using photothermally induced resonance Article de journal

C Policar; J B Waern; M -A Plamont; S Clède; C Mayet; R Prazeres; J -M Ortega; A Vessières; A Dazzi

Angewandte Chemie - International Edition, 50 (4), p. 860–864, 2011.

Proton-coupled electron transfers in biomimetic water bound metal complexes. the electrochemical approach Article de journal

E Anxolabéhère-Mallart; C Costentin; C Policar; M Robert; J -M Savéant; A -L Teillout

Faraday Discussions, 148 , p. 83–95, 2011.

Intrinsically fluorescent glycoligands to study metal selectivity Article de journal

L Garcia; S Maisonneuve; J Oudinet-Sin Marcu; R Guillot; F Lambert; J Xie; C Policar

Inorganic Chemistry, 50 (22), p. 11353–11362, 2011.

2010

Sugars to control ligand shape in metal complexes: Conformationally constrained glycoligands with a predetermination of stereochemistry and a structural control Article de journal

L Garcia; S Maisonneuve; J Xie; R Guillot; P Dorlet; E Rivière; M Desmadril; F Lambert; C Policar

Inorganic Chemistry, 49 (16), p. 7282–7288, 2010.

2009

Design, self-assembly, and molecular structures of 3D copper(II) capsules templated by BF4 - guest anions Article de journal

C Desmarets; C Policar; L -M Chamoreau; H Amouri

European Journal of Inorganic Chemistry, (29-30), p. 4396–4400, 2009.

0000

A di-Copper Peptidyl Complex Mimics the Activity of Catalase, a Key Antioxidant Metalloenzyme Article de journal

Koudedja Coulibaly; Marion Thauvin; Adyn Melenbacher; Clara Testard; Evangelia Trigoni; Amandine Vincent; Martin J Stillman; Sophie Vriz; Clotilde Policar; Nicolas Delsuc

Inorganic Chemistry, 0 (0), p. null, 0000, (PMID: 34109781).

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