2014
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Nitric oxide-triggered remodeling of chloroplast bioenergetics and thylakoid proteins upon nitrogen starvation in Chlamydomonas reinhardtii Article de journal L Wei; B Derrien; A Gautier; L Houille-Vernes; A Boulouis; D Saint-Marcoux; A Malnoë; F Rappaport; C de Vitry; O Vallon; Y Choquet; F -A Wollman Plant Cell, 26 (1), p. 353–372, 2014. @article{Wei:2014,
title = {Nitric oxide-triggered remodeling of chloroplast bioenergetics and thylakoid proteins upon nitrogen starvation in Chlamydomonas reinhardtii},
author = {L Wei and B Derrien and A Gautier and L Houille-Vernes and A Boulouis and D Saint-Marcoux and A Malno\"{e} and F Rappaport and C de Vitry and O Vallon and Y Choquet and F -A Wollman},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84896899282&doi=10.1105%2ftpc.113.120121&partnerID=40&md5=8034415ad66ae55cbf0adb1772a0a7c4},
doi = {10.1105/tpc.113.120121},
year = {2014},
date = {2014-01-01},
journal = {Plant Cell},
volume = {26},
number = {1},
pages = {353--372},
abstract = {Starving microalgae for nitrogen sources is commonly used as a biotechnological tool to boost storage of reduced carbon into starch granules or lipid droplets, but the accompanying changes in bioenergetics have been little studied so far. Here, we report that the selective depletion of Rubisco and cytochrome b6f complex that occurs when Chlamydomonas reinhardtii is starved for nitrogen in the presence of acetate and under normoxic conditions is accompanied by a marked increase in chlororespiratory enzymes, which converts the photosynthetic thylakoid membrane into an intracellular matrix for oxidative catabolism of reductants. Cytochrome b6f subunits and most proteins specifically involved in their biogenesis are selectively degraded, mainly by the FtsH and Clp chloroplast proteases. This regulated degradation pathway does not require light, active photosynthesis, or state transitions but is prevented when respiration is impaired or under phototrophic conditions. We provide genetic and pharmacological evidence that NO production from intracellular nitrite governs this degradation pathway: Addition of a NO scavenger and of two distinct NO producers decrease and increase, respectively, the rate of cytochrome b6f degradation; NO-sensitive fluorescence probes, visualized by confocal microscopy, demonstrate that nitrogen-starved cells produce NO only when the cytochrome b6f degradation pathway is activated. © 2014 American Society of Plant Biologists.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Starving microalgae for nitrogen sources is commonly used as a biotechnological tool to boost storage of reduced carbon into starch granules or lipid droplets, but the accompanying changes in bioenergetics have been little studied so far. Here, we report that the selective depletion of Rubisco and cytochrome b6f complex that occurs when Chlamydomonas reinhardtii is starved for nitrogen in the presence of acetate and under normoxic conditions is accompanied by a marked increase in chlororespiratory enzymes, which converts the photosynthetic thylakoid membrane into an intracellular matrix for oxidative catabolism of reductants. Cytochrome b6f subunits and most proteins specifically involved in their biogenesis are selectively degraded, mainly by the FtsH and Clp chloroplast proteases. This regulated degradation pathway does not require light, active photosynthesis, or state transitions but is prevented when respiration is impaired or under phototrophic conditions. We provide genetic and pharmacological evidence that NO production from intracellular nitrite governs this degradation pathway: Addition of a NO scavenger and of two distinct NO producers decrease and increase, respectively, the rate of cytochrome b6f degradation; NO-sensitive fluorescence probes, visualized by confocal microscopy, demonstrate that nitrogen-starved cells produce NO only when the cytochrome b6f degradation pathway is activated. © 2014 American Society of Plant Biologists. |
2013
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Photochemical properties of Spinach and its use in selective imaging Article de journal P Wang; J Querard; S Maurin; S S Nath; T Le Saux; A Gautier; L Jullien Chemical Science, 4 (7), p. 2865–2873, 2013. @article{Wang:2013a,
title = {Photochemical properties of Spinach and its use in selective imaging},
author = {P Wang and J Querard and S Maurin and S S Nath and T Le Saux and A Gautier and L Jullien},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878785970&doi=10.1039%2fc3sc50729g&partnerID=40&md5=1deeb79d383d404915eba832d5c4b9a6},
doi = {10.1039/c3sc50729g},
year = {2013},
date = {2013-01-01},
journal = {Chemical Science},
volume = {4},
number = {7},
pages = {2865--2873},
abstract = {The progress in imaging instrumentation and probes has revolutionized the way biologists look at living systems. Current tools enable both observation and quantification of biomolecules, allowing the measurement of their complex spatial organization and the dynamic processes in which they are involved. Here, we report reversible photoconversion in the Spinach system, a recently described fluorescent probe for RNA imaging. Upon irradiation with blue light, the Spinach system undergoes photoconversion to a less fluorescent state and fully recovers its signal in the dark. Through thermodynamic titration, stopped-flow, and light-jump experiments, we propose a dynamic model that accounts for the photochemical behavior of the Spinach system. We exploit the dynamic fluorogen exchange and the unprecedented photoconversion properties in a non-covalent fluorescence turn-on system to significantly improve signal-to-background ratio during long-term microscopy imaging. © 2013 Royal Society of Chemistry.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The progress in imaging instrumentation and probes has revolutionized the way biologists look at living systems. Current tools enable both observation and quantification of biomolecules, allowing the measurement of their complex spatial organization and the dynamic processes in which they are involved. Here, we report reversible photoconversion in the Spinach system, a recently described fluorescent probe for RNA imaging. Upon irradiation with blue light, the Spinach system undergoes photoconversion to a less fluorescent state and fully recovers its signal in the dark. Through thermodynamic titration, stopped-flow, and light-jump experiments, we propose a dynamic model that accounts for the photochemical behavior of the Spinach system. We exploit the dynamic fluorogen exchange and the unprecedented photoconversion properties in a non-covalent fluorescence turn-on system to significantly improve signal-to-background ratio during long-term microscopy imaging. © 2013 Royal Society of Chemistry. |
2012
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"Self-immolative" spacer for uncaging with fluorescence reporting Article de journal R Labruère; A Alouane; T Le Saux; I Aujard; P Pelupessy; A Gautier; S Dubruille; F Schmidt; L Jullien Angewandte Chemie - International Edition, 51 (37), p. 9344–9347, 2012. @article{Labruere:2012,
title = {"Self-immolative" spacer for uncaging with fluorescence reporting},
author = {R Labru\`{e}re and A Alouane and T Le Saux and I Aujard and P Pelupessy and A Gautier and S Dubruille and F Schmidt and L Jullien},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84865847445&doi=10.1002%2fanie.201204032&partnerID=40&md5=b61369ac1effc61c29106c6a64c6cd44},
doi = {10.1002/anie.201204032},
year = {2012},
date = {2012-01-01},
journal = {Angewandte Chemie - International Edition},
volume = {51},
number = {37},
pages = {9344--9347},
abstract = {Dual photoliberation: A caged, branched, self-immolative spacer (see scheme, gray box) was designed to rapidly and simultaneously release a desired compound (green) and a fluorophore (red) upon photoactivation. Careful kinetic analysis of the disassembly of the spacer shows that it occurs on the shortest time scale reported to date. © 2012 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Dual photoliberation: A caged, branched, self-immolative spacer (see scheme, gray box) was designed to rapidly and simultaneously release a desired compound (green) and a fluorophore (red) upon photoactivation. Careful kinetic analysis of the disassembly of the spacer shows that it occurs on the shortest time scale reported to date. © 2012 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Modification-Free Photocontrol of $beta$-Lactam Conversion with Spatiotemporal Resolution Article de journal Anna Venancio-Marques; Yan-Jun Liu; Antoine Diguet; Thomas di Maio; Arnaud Gautier; Damien Baigl ACS Synthetic Biology, 1 (11), p. 526-531, 2012. @article{RN49,
title = {Modification-Free Photocontrol of $beta$-Lactam Conversion with Spatiotemporal Resolution},
author = {Anna {Venancio-Marques} and Yan-Jun Liu and Antoine Diguet and Thomas {di Maio} and Arnaud Gautier and Damien Baigl},
doi = {10.1021/sb300010a},
year = {2012},
date = {2012-01-01},
journal = {ACS Synthetic Biology},
volume = {1},
number = {11},
pages = {526-531},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2011
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Light-Activated Kinases Enable Temporal Dissection of Signaling Networks in Living Cells Article de journal Arnaud Gautier; Alexander Deiters; Jason W Chin Journal of the American Chemical Society, 133 (7), p. 2124-2127, 2011, ISSN: 0002-7863. @article{RN41,
title = {Light-Activated Kinases Enable Temporal Dissection of Signaling Networks in Living Cells},
author = {Arnaud Gautier and Alexander Deiters and Jason W Chin},
doi = {10.1021/ja1109979},
issn = {0002-7863},
year = {2011},
date = {2011-01-01},
journal = {Journal of the American Chemical Society},
volume = {133},
number = {7},
pages = {2124-2127},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2010
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Genetically Encoded Photocontrol of Protein Localization in Mammalian Cells Article de journal Arnaud Gautier; Duy P Nguyen; Hrvoje Lusic; Wenlin An; Alexander Deiters; Jason W Chin Journal of the American Chemical Society, 132 (12), p. 4086-4088, 2010, ISSN: 0002-7863. @article{RN40,
title = {Genetically Encoded Photocontrol of Protein Localization in Mammalian Cells},
author = {Arnaud Gautier and Duy P Nguyen and Hrvoje Lusic and Wenlin An and Alexander Deiters and Jason W Chin},
doi = {10.1021/ja910688s},
issn = {0002-7863},
year = {2010},
date = {2010-01-01},
journal = {Journal of the American Chemical Society},
volume = {132},
number = {12},
pages = {4086-4088},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2009
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Selective Cross-Linking of Interacting Proteins Using Self-Labeling Tags Article de journal Arnaud Gautier; Eiji Nakata; Grav zvydas Lukinaviv cius; Kui-Thong Tan; Kai Johnsson Journal of the American Chemical Society, 131 (49), p. 17954-17962, 2009, ISSN: 0002-7863. @article{RN39,
title = {Selective Cross-Linking of Interacting Proteins Using Self-Labeling Tags},
author = {Arnaud Gautier and Eiji Nakata and Gra{v z}vydas Lukinavi{v c}ius and Kui-Thong Tan and Kai Johnsson},
doi = {10.1021/ja907818q},
issn = {0002-7863},
year = {2009},
date = {2009-01-01},
journal = {Journal of the American Chemical Society},
volume = {131},
number = {49},
pages = {17954-17962},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2008
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AGT/SNAP-Tag: A Versatile Tag for Covalent Protein Labeling Article de journal Arnaud Gautier; Kai Johnsson; Helen O'Hare Probes and Tags to Study Biomolecular Function, 2008, ISSN: 9783527623099. @article{RN44,
title = {AGT/SNAP-Tag: A Versatile Tag for Covalent Protein Labeling},
author = {Arnaud Gautier and Kai Johnsson and Helen O'Hare},
doi = {doi:10.1002/9783527623099.ch5 10.1002/9783527623099.ch5},
issn = {9783527623099},
year = {2008},
date = {2008-01-01},
journal = {Probes and Tags to Study Biomolecular Function},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
An Engineered Protein Tag for Multiprotein Labeling in Living Cells Article de journal Arnaud Gautier; Alexandre Juillerat; Christian Heinis; Ivan Reis Corr^ea; Maik Kindermann; Florent Beaufils; Kai Johnsson Chemistry & Biology, 15 (2), p. 128-136, 2008, ISSN: 1074-5521. @article{RN48,
title = {An Engineered Protein Tag for Multiprotein Labeling in Living Cells},
author = {Arnaud Gautier and Alexandre Juillerat and Christian Heinis and Ivan Reis Corr{^e}a and Maik Kindermann and Florent Beaufils and Kai Johnsson},
doi = {https://doi.org/10.1016/j.chembiol.2008.01.007},
issn = {1074-5521},
year = {2008},
date = {2008-01-01},
journal = {Chemistry & Biology},
volume = {15},
number = {2},
pages = {128-136},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2007
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Chemical Probes Shed Light on Protein Function Article de journal Helen M O'Hare; Kai Johnsson; Arnaud Gautier Current Opinion in Structural Biology, 17 (4), p. 488-494, 2007, ISSN: 0959-440X. @article{RN46,
title = {Chemical Probes Shed Light on Protein Function},
author = {Helen M O'Hare and Kai Johnsson and Arnaud Gautier},
doi = {https://doi.org/10.1016/j.sbi.2007.07.005},
issn = {0959-440X},
year = {2007},
date = {2007-01-01},
journal = {Current Opinion in Structural Biology},
volume = {17},
number = {4},
pages = {488-494},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2006
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An Unusual Functional Group Interaction and Its Potential to Reproduce Steric and Electrostatic Features of the Transition States of Peptidolysis Article de journal Arnaud Gautier; Delphine Pitrat; Jens Hasserodt Bioorganic & Medicinal Chemistry, 14 (11), p. 3835-3847, 2006, ISSN: 0968-0896. @article{RN47b,
title = {An Unusual Functional Group Interaction and Its Potential to Reproduce Steric and Electrostatic Features of the Transition States of Peptidolysis},
author = {Arnaud Gautier and Delphine Pitrat and Jens Hasserodt},
doi = {https://doi.org/10.1016/j.bmc.2006.01.031},
issn = {0968-0896},
year = {2006},
date = {2006-01-01},
journal = {Bioorganic & Medicinal Chemistry},
volume = {14},
number = {11},
pages = {3835-3847},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2005
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Chiral Trialkanolamine-Based Hemicryptophanes: Synthesis and Oxovanadium Complex Article de journal A Gautier; J C Mulatier; J Crassous; J P Dutasta Organic Letters, 7 (7), p. 1207-1210, 2005, ISSN: 1523-7060. @article{RN51b,
title = {Chiral Trialkanolamine-Based Hemicryptophanes: Synthesis and Oxovanadium Complex},
author = {A Gautier and J C Mulatier and J Crassous and J P Dutasta},
doi = {10.1021/ol047469+},
issn = {1523-7060},
year = {2005},
date = {2005-01-01},
journal = {Organic Letters},
volume = {7},
number = {7},
pages = {1207-1210},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
