Christophe TRIBET

Directeur de Recherche, CNRS

ENS – Département de chimie
24 rue Lhomond, 75005 Paris

Email: christophe.tribet@ens.psl.eu
Phone: +33 144322403
Office: E152

ORCID: http://orcid.org/0000-0002-5953-0968

Researchgate:https://www.researchgate.net/profile/Christophe_Tribet

Short bio

Based on a core expertise in physical chemistry of polymer and surfactants, and soft matter science the team explore in tight collaboration with biologists, the design of stimuli-responsive (macro)molecular tools 1) in biochemistry and 2) in cell biology

With J.L. Popot in IBPC in the late 90’s, we tailored macromolecular detergents that enable the dispersion and stabilization of integral membrane proteins in water. These polymers are now quoted « amphipols » and commercially available.  Since this early work, I continuously explored the use of amphiphilic assemblies (polymers, surfactants) under the form of complexes (coll. F. Winnik) or coulombic assemblies and coacervates (coll. P. Dubin), or hydrogels to control protein dispersion, to prevent aggregation, enhance refolding/renaturation.

In SIMM laboratory (1994-2009, ESPCI Paris), we introduced chemical modifications in these systems  aiming to achieve stimuli-responsive, complex transitions (e.g. coacervation, physical gelation, emulsification). We investigated the use of photochrome-containing macromolecules, to modulate on demand polymer assemblies. We achieved photo-triggered switch of gels, of emulsion (coll. P.Perrin) and foams stability (coll. C. Monteux). As regard biological perspectives, this approach enabled us to trigger the permeability of bilayers and cells to various substrates (peptides, Qdots) in collaboration with L. Auvray, S. Cribier, D. Massotte, S. Sagan.

Recent works are aimed to provide dynamic switches (based on light, temperature or redox potential) on the surface of cell-culture substrates, and on lipid membranes. With E. Marie and D. Zanchi,  we study how cell fate can be oriented on spatially micropatterned substrates (soft adhesion, polarisation, migration) by tailoring and in-situ modulation of cell-polymer interactions. Finally, we seek to develop biosourced forms of these tools. With B. Miroux (IBPC), we aim to obtain responsive membranes and mimics of biological compartments (artificial organelles) from bacteria mutants expressing ad hoc (genetically encoded) sets of proteins and lipids.

The development of robust tools, that can be implemented by biologists in representative biological conditions, is a major concern inspiring all our work.

Education and professional experience

  •  2010 – , Senior CNRS Research Scientist, « Polymer » group in Pole « Physical & Biological Chemistry of Living Matter », ENS Paris,
  •  2007-2010, Senior Research Scientist, team « stimuli-responsive colloids », SIMM ESPCI Paris,
  •  2005 Visiting Scientist, Université de Montreal (F. Winnik),
  • 1994 CNRS Research Scientist , ESPCI Paris,
  • 1993 Post-doctoral fellow, ULB, Bruxelles,
  • 1993 Ph. D. Université Paris VI
  • 1985/1987  B.Sc. & M.Sc. chemistry, Univ. Paris XI Orsay & ENS Cachan, France

Research interests

  • Physical chemistry of protein aggregation/stability (ex: therapeutic antibodies),
  • Polymer:biomembrane & polymer:protein assemblies (chaperon-like polymers, genetically encoded capsules),
  • Dynamic polymer substrates, cell guidance, remote control of cell adhesion, permeabilization.

Supervised students and post-doctorants

Teaching

  • Module coordinator « physico-chimie des polymères », predoc ENS chimie (eq. M1),
  • Module coordinator « handling of proteins and biomembranes », M2, master BME Biomaterial engineering, PSL Univ.,
  • M2 course, UE NC862 « biomaterial », Univ. Paris VI.

Recent publications

– Royes J.; Ilioaia O.; Lubart Q.; Dubacheva G.; Bally M.; Miroux B.; Tribet C., Angew. Chem. Int. Ed., 2019, doi: 10.1002/anie.201902929, Protein-loaded Lipidic Nanocapsules from Bio-engineered Bacteria.

– Boniello G.; Tribet C.; Marie E.; Croquette V.; Zanchi D., Phys Rev E, 2018 LJ15381E, Rolling and aging in T-ramp soft adhesion.

– Dalier F.; Dubacheva G.; Coniel M.; Galtayries A.; Marie E.; Piel M.; Tribet C., ACS Applied Materials & Interf., 2018, 10(3):2253-2258, Mixed copolymer adlayers allowing reversible thermal control of single cell aspect ratio.

– Martin N.; Costa N.; Wien F.; Winnik F.; Ortega C.; Herbet A.; Boquet D.; Tribet C., Macromolecular Biosciences, 2017, 17 (2) UNSP 1600213 DOI:10.1002/ mabi.201600213, Refolding of Aggregation-Prone ScFv Antibody Fragments Assisted by Hydrophobically Modified Poly(sodium acrylate) Derivatives.

– Lund R., Brun G., Chevallier E., Narayanan Theyencheri, Tribet C., Langmuir, 2016, 32 (11), 2539–2548, Kinetics of Photocontrollable Micelles: Light-Induced Self-Assembly and Disassembly of Azobenzene-Based Surfactants Revealed by TR-SAXS.

– Dalier F., Eghiaian F., Scheuring S., Marie E., Tribet C., Biomacromolecules, 2016, 17 (5), , 1727–1736, Temperature-Switchable Control of Ligand Display on Adlayers of Mixed Poly(lysine)-g-(PEO) and Poly(lysine)-g-(ligand-modified poly-N-isopropylacrylamide).

– Marie, E.; Sagan, S.; Cribier, S.; Tribet, C., J. Membrane Biol;, 2014, 247, 861-881 , Amphiphilic Macromolecules on Cell Membranes: From Protective Layers to Controlled Permeabilization.

– Stijn F. M. van Dongen, P. Maiuri, E. Marie, C. Tribet and M. Piel, Adv. Mater. 2013, 25(12):1687-91, Triggering Cell Adhesion, Migration or Shape Change with a Dynamic Surface Coating.

– E. Chevallier, C. Monteux, F. Lequeux, C. Tribet, Langmuir 2012, 28(5), 2308-2312. Photofoamers : Remote Control of Foam Destabilization by Exposure to Light Using an Azobenzene Surfactant.

 

Publications

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2020

Solubilization and Stabilization of Membrane Proteins by Cycloalkane-Modified Amphiphilic Polymers Article de journal

Anaïs Marconnet; Baptiste Michon; Christel Le Bon; Fabrice Giusti; Christophe Tribet; Manuela Zoonens

Biomacromolecules, 21 (8), p. 3459-3467, 2020, ISSN: 1525-7797.

Liens

2019

Bacteria-Based Production of Thiol-Clickable, Genetically Encoded Lipid Nanovesicles Article de journal

Jorge Royes; Oana Ilioaia; Quentin Lubart; Federica Angius; Galina V Dubacheva; Marta Bally; Bruno Miroux; Christophe Tribet

Angewandte Chemie International Edition, 58 , p. 7395-7399, 2019, ISSN: 1433-7851.

Liens

Distinctive Low-Resolution Structural Features of Dimers of Antibody–Drug Conjugates and Parent Antibody Determined by Small-Angle X-ray Scattering Article de journal

Didier Law-Hine; Sergii Rudiuk; Audrey Bonestebe; Romain Ienco; Sylvain Huille; Christophe Tribet

Mol. Pharmaceutics, 16 (12), p. 4902-4912, 2019.

Liens

Redox-Triggered Control of Cell Adhesion and Deadhesion on Poly(lysine)-g-poly(ethylene oxide) Adlayers. Article de journal

Louise Hespel; Julien Dupré de Baubigny; Pierre Lalanne; Simon de Beco; Mathieu Coppey; Catherine Villard; Vincent Humblot; Emmanuelle Marie; Christophe Tribet

ACS Applied Bio Materials, 2 (10), p. 4367-4376, 2019.

Liens

Tunable and switchable soft adsorption of polymer-coated microparticles on a flat substrate Article de journal

Giuseppe Boniello; Christophe Tribet; Emmanuelle Marie; Vincent Croquette; Dražen Zanchi

Colloids and Surfaces A: Physicochemical and Engineering Aspects, 575 , p. 199-204, 2019, ISSN: 0927-7757.

Liens

2018

Mixed Copolymer Adlayers Allowing Reversible Thermal Control of Single Cell Aspect Ratio Article de journal

F Dalier; G V Dubacheva; M Coniel; D Zanchi; A Galtayries; M Piel; E Marie; C Tribet

Acs Applied Materials & Interfaces, 10 (3), p. 2253-2258, 2018, ISSN: 1944-8244.

Résumé | Liens

Rolling and aging in temperature-ramp soft adhesion Article de journal

G Boniello; C Tribet; E Marie; V Croquette; D Zanchi

Physical Review E, 97 (1), 2018.

Résumé | Liens

2017

Refolding of Aggregation-Prone ScFv Antibody Fragments Assisted by Hydrophobically Modified Poly(sodium acrylate) Derivatives Article de journal

N Martin; N Costa; F Wien; F M Winnik; C Ortega; A Herbet; D Boquet; C Tribet

Macromolecular Bioscience, 17 (2), 2017.

Résumé | Liens

Reversible and Dynamical Control of Aggregation and Soft Adhesion of Ŧ-Responsive Polymer-Coated Colloids Article de journal

Giuseppe Boniello; Jeremy Malinge; Christophe Tribet; Emmanuelle Marie; Drazen Zanchi

Colloids and Surfaces a-Physicochemical and Engineering Aspects, 532 , p. 510-515, 2017, ISSN: 0927-7757.

Liens

2016

Aggregation of Antibody Drug Conjugates at Room Temperature: SAXS and Light Scattering Evidence for Colloidal Instability of a Specific Subpopulation Article de journal

B Frka-Petesic; D Zanchi; N Martin; S Carayon; S Huille; C Tribet

Langmuir, 32 (19), p. 4848–4861, 2016.

Résumé | Liens

Kinetics of Photocontrollable Micelles: Light-Induced Self-Assembly and Disassembly of Azobenzene-Based Surfactants Revealed by TR-SAXS Article de journal

R Lund; G Brun; E Chevallier; T Narayanan; C Tribet

Langmuir, 32 (11), p. 2539–2548, 2016.

Résumé | Liens

Tailored stimuli-responsive interaction between particles adjusted by straightforward adsorption of mixed layers of Poly(lysine)-g-PEG and Poly(lysine)-g-PNIPAM on anionic beads Article de journal

J Malinge; F Mousseau; D Zanchi; G Brun; C Tribet; E Marie

Journal of Colloid and Interface Science, 461 , p. 50–55, 2016.

Résumé | Liens

Temperature-Switchable Control of Ligand Display on Adlayers of Mixed Poly(Lysine)-g-(PEO) and Poly(Lysine)-g-(Ligand-Modified Poly-N-Isopropylacrylamide) Article de journal

F Dalier; F Eghiaian; S Scheuring; E Marie; C Tribet

Biomacromolecules, 17 (5), p. 1727-1736, 2016, ISSN: 1525-7797.

Liens

Temperature-Switchable Control of Ligand Display on Adlayers of Mixed Poly(Lysine)-g-(PEO) and Poly(Lysine)-g-(Ligand-Modified Poly-N-Isopropylacrylamide) Article de journal

F Dalier; F Eghiaian; S Scheuring; E Marie; C Tribet

Biomacromolecules, 17 , p. 1727-1736, 2016, ISSN: 1525-7797.

Liens

2015

Prevention of aggregation and renaturation of carbonic anhydrase via weak association with octadecyl- or azobenzene-modified poly(acrylate) derivatives Article de journal

N Martin; J Ruchmann; C Tribet

Langmuir, 31 (1), p. 338–349, 2015.

Résumé | Liens

2014

Amphiphilic Macromolecules on Cell Membranes: From Protective Layers to Controlled Permeabilization Article de journal

E Marie; S Sagan; S Cribier; C Tribet

Journal of Membrane Biology, 247 (9-10), p. 861–881, 2014.

Résumé | Liens

Photocontrolled ionization in the corona of rodlike assemblies of diblock copolymers Article de journal

J Sun; L Jia; M Emond; M -H Li; E Marie; L Jullien; C Tribet

Macromolecules, 47 (5), p. 1684–1692, 2014.

Résumé | Liens

Prevention of thermally induced aggregation of igg antibodies by noncovalent interaction with poly(acrylate) derivatives Article de journal

N Martin; D Ma; A Herbet; D Boquet; F M Winnik; C Tribet

Biomacromolecules, 15 (8), p. 2952–2962, 2014.

Résumé | Liens

Quantitative characterization by asymmetrical flow field-flow fractionation of IgG thermal aggregation with and without polymer protective agents Article de journal

D Ma; N Martin; C Tribet; F M Winnik

Analytical and Bioanalytical Chemistry, 406 (29), p. 7539–7547, 2014.

Résumé | Liens

2013

Triggering Cell Adhesion, Migration or Shape Change with a Dynamic Surface Coating Article de journal

S F M van Dongen; P Maiuri; E Marie; C Tribet; M Piel

Advanced Materials, 25 (12), p. 1687-1691, 2013, ISSN: 0935-9648.

Résumé | Liens

2012

Importance of the Dynamics of Adsorption and of a Transient Interfacial Stress on the Formation of Aggregates of IgG Antibodies Article de journal

S Rudiuk; L Cohen-Tannoudji; S Huille; C Tribet

Soft Matter, 8 (9), p. 2651-2661, 2012, ISSN: 1744-683X.

Résumé | Liens

Nanoemulsions with Enhanced Temperature Stability Using Thermo-Sensitive Association of Nonionic Surfactant and Amphiphilic Polyelectrolytes Article de journal

J Galindo-Alvarez; V Sadtler; P Marchal; P Perrin; C Tribet; E Marie; A Durand

Colloids and Surfaces a-Physicochemical and Engineering Aspects, 396 , p. 115-121, 2012, ISSN: 0927-7757.

Liens

Non-Monotonous Variation of the LCST of Light-Responsive, Amphiphilic Poly(NIPAM) Derivatives Article de journal

Y J Liu; A Pallier; J Sun; S Rudiuk; D Baigl; M Piel; E Marie; C Tribet

Soft Matter, 8 (32), p. 8446-8455, 2012, ISSN: 1744-683X.

Résumé | Liens

Photocontrol of the translocation of molecules, peptides, and quantum dots through cell and lipid membranes doped with azobenzene copolymers Article de journal

S C Sebai; D Milioni; A Walrant; I D Alves; S Sagan; C Huin; L Auvray; D Massotte; S Cribier; C Tribet

Angewandte Chemie - International Edition, 51 (9), p. 2132–2136, 2012.

Résumé | Liens

Photofoams: Remote control of foam destabilization by exposure to light using an azobenzene surfactant Article de journal

E Chevallier; C Monteux; F Lequeux; C Tribet

Langmuir, 28 (5), p. 2308–2312, 2012.

Résumé | Liens

Photofoams: Remote Control of Foam Destabilization by Exposure to Light Using an Azobenzene Surfactant Article de journal

E Chevallier; C Monteux; F Lequewx; C Tribet

Langmuir, 28 , p. 2308-2312, 2012, ISSN: 0743-7463.

Liens

Reactive Protein-Repellent Surfaces for the Straightforward Attachment of Small Molecules up to Whole Cells Article de journal

S F M van Dongen; J Janvore; S S van Berkel; E Marie; M Piel; C Tribet

Chemical Science, 3 (10), p. 3000-3006, 2012, ISSN: 2041-6520.

Résumé | Liens

Reverse Variation of Cloud Points of Light-Responsive Assemblies of Azobenzene-Modified Amphiphilic Polymers Article de journal

E Marie; C Tribet

Chemistry Letters, 41 , p. 1093-1095, 2012, ISSN: 0366-7022.

Liens

The Thermally Induced Aggregation of Immunoglobulin G in Solution Is Prevented by Amphipols Article de journal

D W Ma; N Martin; A Herbet; D Boquet; C Tribet; F M Winnik

Chemistry Letters, 41 , p. 1380-1382, 2012, ISSN: 0366-7022.

Liens

Unfolding of cytochrome c upon interaction with azobenzene-modified copolymers Article de journal

J Sun; J Ruchmann; A Pallier; L Jullien; M Desmadril; C Tribet

Biomacromolecules, 13 (11), p. 3736–3746, 2012.

Résumé | Liens

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