Chargée de recherche
ENS – Département de chimie
24 rue Lhomond, 75005 Paris
Email: emmanuelle.marie@ens.psl.eu
Phone: 0144322408
Office: E142b
We propose below 8 sections. Every member of the department can choose which one he/she wants to use. You can also use new ones. We only ask everyone to keep the general aspect of the page (no change of font, color, size, etc). For the picture, it must be 250px wide.
Short bio
You can decide to write text or use bullet points as below
Education and professional experience
- Short CV
- List by date
Research interests
- List of keywords and themes
Awards and distinctions
- You can also list the membership to professional organizations (SCF, e.g.)
Supervised students and post-doctorants
- Currents and formers
Teaching
- You can also list teaching materials (we can upload pdf documents to the website)
Significant publications
- You can choose the full list of publications (below) or only selected ones
Publications
2022 |
UCST-Type Polymer Capsules Formed by Interfacial Complexation Article de journal L Sixdenier; A Auge; Y Zhao; E Marie; C Tribet ACS MACRO LETTERS, 11 (5), p. 651-656, 2022, ISBN: 2161-1653 J9 - ACS MACRO LETT. @article{Sixdenier2022, title = {UCST-Type Polymer Capsules Formed by Interfacial Complexation}, author = {L Sixdenier and A Auge and Y Zhao and E Marie and C Tribet}, doi = {10.1021/acsmacrolett.2c00021}, isbn = {2161-1653 J9 - ACS MACRO LETT}, year = {2022}, date = {2022-05-17}, journal = {ACS MACRO LETTERS}, volume = {11}, number = {5}, pages = {651-656}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2021 |
Emulsion-Templated Poly(N-Isopropylacrylamide) Shells Formed by Thermo-Enhanced Interfacial Complexation Article de journal L Sixdenier; C Tribet; E Marie ADVANCED FUNCTIONAL MATERIALS, 31 (51), p. 2105490, 2021, ISBN: 1616-301X 1616-3028 J9 - ADV FUNCT MATER. @article{Sixdenier2021b, title = {Emulsion-Templated Poly(N-Isopropylacrylamide) Shells Formed by Thermo-Enhanced Interfacial Complexation}, author = {L Sixdenier and C Tribet and E Marie}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.202105490}, doi = {10.1002/adfm.202105490}, isbn = {1616-301X 1616-3028 J9 - ADV FUNCT MATER}, year = {2021}, date = {2021-09-17}, journal = {ADVANCED FUNCTIONAL MATERIALS}, volume = {31}, number = {51}, pages = {2105490}, abstract = {The encapsulation of fragile biomacromolecules is crucial in many biotechnological applications but remains challenging. Interfacial complexation (IC) in water-in-oil emulsions proves to be an efficient process for the formation of protective polymer layers at the surface of capsule-precursor water droplets. In this work, the enhancement of conventional IC by introducing thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) strands in the interfacial polymer layer is described. Surfactant-polymer IC is implemented in water-in-fluorocarbon oil emulsions between a water-soluble poly(L-lysine)-g-poly(N-isopropylacrylamide) cationic copolymer (PLL-g-PNIPAM) and an oil-soluble anionic surfactant. Fluorescence imaging demonstrates that the thermal collapse transition of PNIPAM strands, triggered by gentle heating, induces an enrichment of the polymer layer initially formed by IC. Spontaneous co-precipitation of nanoparticles initially dispersed in the aqueous cores-with no specific treatment-is also achieved upon PNIPAM transition. This process is leveraged to irreversibly segregate these nanoparticles in the interfacial polymer layer, resulting in gel-like mixed shells. Thermo-enhancement of conventional IC is thus a promising approach for the straightforward formation, strengthening, and functionalization of capsule shells. As implemented in mild conditions, thermo-enhanced IC is additionally compatible with the encapsulation of proteins, opening new opportunities for delivery systems of biomacromolecules.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The encapsulation of fragile biomacromolecules is crucial in many biotechnological applications but remains challenging. Interfacial complexation (IC) in water-in-oil emulsions proves to be an efficient process for the formation of protective polymer layers at the surface of capsule-precursor water droplets. In this work, the enhancement of conventional IC by introducing thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) strands in the interfacial polymer layer is described. Surfactant-polymer IC is implemented in water-in-fluorocarbon oil emulsions between a water-soluble poly(L-lysine)-g-poly(N-isopropylacrylamide) cationic copolymer (PLL-g-PNIPAM) and an oil-soluble anionic surfactant. Fluorescence imaging demonstrates that the thermal collapse transition of PNIPAM strands, triggered by gentle heating, induces an enrichment of the polymer layer initially formed by IC. Spontaneous co-precipitation of nanoparticles initially dispersed in the aqueous cores-with no specific treatment-is also achieved upon PNIPAM transition. This process is leveraged to irreversibly segregate these nanoparticles in the interfacial polymer layer, resulting in gel-like mixed shells. Thermo-enhancement of conventional IC is thus a promising approach for the straightforward formation, strengthening, and functionalization of capsule shells. As implemented in mild conditions, thermo-enhanced IC is additionally compatible with the encapsulation of proteins, opening new opportunities for delivery systems of biomacromolecules. |
2019 |
Redox-Triggered Control of Cell Adhesion and Deadhesion on Poly(lysine)-g-poly(ethylene oxide) Adlayers. Article de journal Louise Hespel; Julien Dupré de Baubigny; Pierre Lalanne; Simon de Beco; Mathieu Coppey; Catherine Villard; Vincent Humblot; Emmanuelle Marie; Christophe Tribet ACS Applied Bio Materials, 2 (10), p. 4367-4376, 2019. @article{Hespel2019, title = {Redox-Triggered Control of Cell Adhesion and Deadhesion on Poly(lysine)-g-poly(ethylene oxide) Adlayers.}, author = {Louise Hespel and Julien Dupr\'{e} de Baubigny and Pierre Lalanne and Simon de Beco and Mathieu Coppey and Catherine Villard and Vincent Humblot and Emmanuelle Marie and Christophe Tribet}, doi = {10.1021/acsabm.9b00601}, year = {2019}, date = {2019-09-10}, journal = {ACS Applied Bio Materials}, volume = {2}, number = {10}, pages = {4367-4376}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Tunable and switchable soft adsorption of polymer-coated microparticles on a flat substrate Article de journal Giuseppe Boniello; Christophe Tribet; Emmanuelle Marie; Vincent Croquette; Dražen Zanchi Colloids and Surfaces A: Physicochemical and Engineering Aspects, 575 , p. 199-204, 2019, ISSN: 0927-7757. @article{Boniello2019, title = {Tunable and switchable soft adsorption of polymer-coated microparticles on a flat substrate}, author = {Giuseppe Boniello and Christophe Tribet and Emmanuelle Marie and Vincent Croquette and Dra\v{z}en Zanchi}, editor = {Elsevier}, url = {http://www.sciencedirect.com/science/article/pii/S0927775719301797}, doi = {10.1016/j.colsurfa.2019.04.081}, issn = {0927-7757}, year = {2019}, date = {2019-08-20}, journal = {Colloids and Surfaces A: Physicochemical and Engineering Aspects}, volume = {575}, pages = {199-204}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2018 |
Mixed Copolymer Adlayers Allowing Reversible Thermal Control of Single Cell Aspect Ratio Article de journal F Dalier; G V Dubacheva; M Coniel; D Zanchi; A Galtayries; M Piel; E Marie; C Tribet Acs Applied Materials & Interfaces, 10 (3), p. 2253-2258, 2018, ISSN: 1944-8244. @article{Dalier:2018, title = {Mixed Copolymer Adlayers Allowing Reversible Thermal Control of Single Cell Aspect Ratio}, author = {F Dalier and G V Dubacheva and M Coniel and D Zanchi and A Galtayries and M Piel and E Marie and C Tribet}, doi = {10.1021/acsami.7b18513}, issn = {1944-8244}, year = {2018}, date = {2018-01-01}, journal = {Acs Applied Materials & Interfaces}, volume = {10}, number = {3}, pages = {2253-2258}, abstract = {Dynamic guidance of living cells is achieved by fine-tuning and spatiotemporal modulation on artificial polymer layers enabling reversible peptide display. Adjustment of surface composition and interactions is obtained by coadsorption of mixed poly(lysine) derivatives, grafted with either repellent PEG, RGD adhesion peptides, or T-responsive poly(N-isopropylacrylamide) strands. Deposition of mixed adlayers provides a straightforward mean to optimize complex substrates, which is here implemented to achieve (1) thermal control of ligand accessibility and (2) adjustment of relative adhesiveness between adjacent micropatterns, while preserving cell attachment during thermal cycles. The reversible polarization of HeLa cells along orthogonal stripes mimics guidance along natural matrices.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dynamic guidance of living cells is achieved by fine-tuning and spatiotemporal modulation on artificial polymer layers enabling reversible peptide display. Adjustment of surface composition and interactions is obtained by coadsorption of mixed poly(lysine) derivatives, grafted with either repellent PEG, RGD adhesion peptides, or T-responsive poly(N-isopropylacrylamide) strands. Deposition of mixed adlayers provides a straightforward mean to optimize complex substrates, which is here implemented to achieve (1) thermal control of ligand accessibility and (2) adjustment of relative adhesiveness between adjacent micropatterns, while preserving cell attachment during thermal cycles. The reversible polarization of HeLa cells along orthogonal stripes mimics guidance along natural matrices. |
Rolling and aging in temperature-ramp soft adhesion Article de journal G Boniello; C Tribet; E Marie; V Croquette; D Zanchi Physical Review E, 97 (1), 2018. @article{Boniello:2018, title = {Rolling and aging in temperature-ramp soft adhesion}, author = {G Boniello and C Tribet and E Marie and V Croquette and D Zanchi}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040729302&doi=10.1103%2fPhysRevE.97.012609&partnerID=40&md5=5b4fb855a186c8f56070f77a7d75f5c8}, doi = {10.1103/PhysRevE.97.012609}, year = {2018}, date = {2018-01-01}, journal = {Physical Review E}, volume = {97}, number = {1}, abstract = {Immediately before adsorption to a horizontal substrate, sinking polymer-coated colloids can undergo a complex sequence of landing, jumping, crawling, and rolling events. Using video tracking, we studied the soft adhesion to a horizontal flat plate of micron-size colloids coated by a controlled molar fraction f of the poly(lysine)-grafted-poly(N-isopropylacrylamide) (PLL-g-PNIPAM) which is a temperature-sensitive polymer. We ramp the temperature from below to above Tc=32±1C, at which the PNIPAM polymer undergoes a transition, triggering attractive interaction between microparticles and surface. The adsorption rate, the effective in-plane (x-y) diffusion constant, and the average residence time distribution over z were extracted from the Brownian motion records during last seconds before immobilization. Experimental data are understood within a rate-equations-based model that includes aging effects and includes three populations: the untethered, the rolling, and the arrested colloids. We show that preadsorption dynamics casts a characteristic scaling function α(f) proportional to the number of available PNIPAM patches met by soft contact during Brownian rolling. In particular, the increase of in-plane diffusivity with increasing f is understood: The stickiest particles have the shortest rolling regime prior to arrest, so that their motion is dominated by the untethered phase. © 2018 American Physical Society.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Immediately before adsorption to a horizontal substrate, sinking polymer-coated colloids can undergo a complex sequence of landing, jumping, crawling, and rolling events. Using video tracking, we studied the soft adhesion to a horizontal flat plate of micron-size colloids coated by a controlled molar fraction f of the poly(lysine)-grafted-poly(N-isopropylacrylamide) (PLL-g-PNIPAM) which is a temperature-sensitive polymer. We ramp the temperature from below to above Tc=32±1C, at which the PNIPAM polymer undergoes a transition, triggering attractive interaction between microparticles and surface. The adsorption rate, the effective in-plane (x-y) diffusion constant, and the average residence time distribution over z were extracted from the Brownian motion records during last seconds before immobilization. Experimental data are understood within a rate-equations-based model that includes aging effects and includes three populations: the untethered, the rolling, and the arrested colloids. We show that preadsorption dynamics casts a characteristic scaling function α(f) proportional to the number of available PNIPAM patches met by soft contact during Brownian rolling. In particular, the increase of in-plane diffusivity with increasing f is understood: The stickiest particles have the shortest rolling regime prior to arrest, so that their motion is dominated by the untethered phase. © 2018 American Physical Society. |
2017 |
Reversible and Dynamical Control of Aggregation and Soft Adhesion of Ŧ-Responsive Polymer-Coated Colloids Article de journal Giuseppe Boniello; Jeremy Malinge; Christophe Tribet; Emmanuelle Marie; Drazen Zanchi Colloids and Surfaces a-Physicochemical and Engineering Aspects, 532 , p. 510-515, 2017, ISSN: 0927-7757. @article{RN3h, title = {Reversible and Dynamical Control of Aggregation and Soft Adhesion of {T}-Responsive Polymer-Coated Colloids}, author = {Giuseppe Boniello and Jeremy Malinge and Christophe Tribet and Emmanuelle Marie and Drazen Zanchi}, doi = {10.1016/j.colsurfa.2017.04.011}, issn = {0927-7757}, year = {2017}, date = {2017-01-01}, journal = {Colloids and Surfaces a-Physicochemical and Engineering Aspects}, volume = {532}, pages = {510-515}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2016 |
J Malinge; F Mousseau; D Zanchi; G Brun; C Tribet; E Marie Journal of Colloid and Interface Science, 461 , p. 50–55, 2016. @article{Malinge:2016, title = {Tailored stimuli-responsive interaction between particles adjusted by straightforward adsorption of mixed layers of Poly(lysine)-g-PEG and Poly(lysine)-g-PNIPAM on anionic beads}, author = {J Malinge and F Mousseau and D Zanchi and G Brun and C Tribet and E Marie}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84941966040&doi=10.1016%2fj.jcis.2015.09.016&partnerID=40&md5=5f2229f36d05f3279fd26b0c88f46406}, doi = {10.1016/j.jcis.2015.09.016}, year = {2016}, date = {2016-01-01}, journal = {Journal of Colloid and Interface Science}, volume = {461}, pages = {50--55}, abstract = {We report a simple and versatile method to functionalize anionic colloid particles and control particle solubility. Poly(lysine)-based copolymers (PLL) grafted with polyethylene oxide (PLL-g-PEG) or poly(N-isopropylacrylamide) (PLL-g-PNIPAM) spontaneously adsorb on bare beads dispersed in aqueous solutions of the copolymers. The final composition of the mixed ad-layers formed (i.e. PEG/PNIPAM ratio) was adjusted by the polymer concentrations in solutions. While the (PLL-g-PEG)-coated particles were stable in a wide range of temperature, the presence of PLL-g-PNIPAM in the outer layer provided a reversible temperature-triggered aggregation at 32 ± 1. °C. In the range of PNIPAM fraction going from 100% (beads fully covered by PLL-g-PNIPAM) down to a threshold 20% weight ratio (with 80% PLL-g-PEG), the particles aggregated rapidly to form micrometer size clusters. Below 20% weight fraction of PLL-g-PNIPAM, the kinetic was drastically lowered. Using PLL derivatives provides a straightforward route allowing to control the fraction of a functional chain (here PNIPAM) deposited on PEGylated particles, and in turn to adjust surface interaction and here the rate of particle-particle aggregation as a function of the density of functional chains. This approach can be generalized to many anionic surfaces onto which PLL is known to adhere tightly, such as glass or silica. © 2015 Elsevier Inc.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We report a simple and versatile method to functionalize anionic colloid particles and control particle solubility. Poly(lysine)-based copolymers (PLL) grafted with polyethylene oxide (PLL-g-PEG) or poly(N-isopropylacrylamide) (PLL-g-PNIPAM) spontaneously adsorb on bare beads dispersed in aqueous solutions of the copolymers. The final composition of the mixed ad-layers formed (i.e. PEG/PNIPAM ratio) was adjusted by the polymer concentrations in solutions. While the (PLL-g-PEG)-coated particles were stable in a wide range of temperature, the presence of PLL-g-PNIPAM in the outer layer provided a reversible temperature-triggered aggregation at 32 ± 1. °C. In the range of PNIPAM fraction going from 100% (beads fully covered by PLL-g-PNIPAM) down to a threshold 20% weight ratio (with 80% PLL-g-PEG), the particles aggregated rapidly to form micrometer size clusters. Below 20% weight fraction of PLL-g-PNIPAM, the kinetic was drastically lowered. Using PLL derivatives provides a straightforward route allowing to control the fraction of a functional chain (here PNIPAM) deposited on PEGylated particles, and in turn to adjust surface interaction and here the rate of particle-particle aggregation as a function of the density of functional chains. This approach can be generalized to many anionic surfaces onto which PLL is known to adhere tightly, such as glass or silica. © 2015 Elsevier Inc. |
Temperature-Switchable Control of Ligand Display on Adlayers of Mixed Poly(Lysine)-g-(PEO) and Poly(Lysine)-g-(Ligand-Modified Poly-N-Isopropylacrylamide) Article de journal F Dalier; F Eghiaian; S Scheuring; E Marie; C Tribet Biomacromolecules, 17 (5), p. 1727-1736, 2016, ISSN: 1525-7797. @article{Dalier:2016, title = {Temperature-Switchable Control of Ligand Display on Adlayers of Mixed Poly(Lysine)-g-(PEO) and Poly(Lysine)-g-(Ligand-Modified Poly-N-Isopropylacrylamide)}, author = {F Dalier and F Eghiaian and S Scheuring and E Marie and C Tribet}, doi = {10.1021/acs.biomac.6b00136}, issn = {1525-7797}, year = {2016}, date = {2016-05-01}, journal = {Biomacromolecules}, volume = {17}, number = {5}, pages = {1727-1736}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Temperature-Switchable Control of Ligand Display on Adlayers of Mixed Poly(Lysine)-g-(PEO) and Poly(Lysine)-g-(Ligand-Modified Poly-N-Isopropylacrylamide) Article de journal F Dalier; F Eghiaian; S Scheuring; E Marie; C Tribet Biomacromolecules, 17 , p. 1727-1736, 2016, ISSN: 1525-7797. @article{RN4d, title = {Temperature-Switchable Control of Ligand Display on Adlayers of Mixed Poly(Lysine)-g-(PEO) and Poly(Lysine)-g-(Ligand-Modified Poly-N-Isopropylacrylamide)}, author = {F Dalier and F Eghiaian and S Scheuring and E Marie and C Tribet}, doi = {10.1021/acs.biomac.6b00136}, issn = {1525-7797}, year = {2016}, date = {2016-01-01}, journal = {Biomacromolecules}, volume = {17}, pages = {1727-1736}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2015 |
Kinetics of formation of oil-in-water emulsions using in situ rheo-optical measurements Article de journal R Covis; E Marie; A Durand; C Baravian AIChE Journal, 61 (1), p. 277–284, 2015. @article{Covis:2015a, title = {Kinetics of formation of oil-in-water emulsions using in situ rheo-optical measurements}, author = {R Covis and E Marie and A Durand and C Baravian}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84920019195&doi=10.1002%2faic.14626&partnerID=40&md5=e7f1a416a2ac1f9fb32befa9f84b8812}, doi = {10.1002/aic.14626}, year = {2015}, date = {2015-01-01}, journal = {AIChE Journal}, volume = {61}, number = {1}, pages = {277--284}, abstract = {The kinetics of mechanical emulsification in vane geometry was investigated using an original rheo-optic device. Hexadecane-in-water micronic emulsions were prepared using a nonionic polymeric surfactant (Brij700®) as stabilizer. The viscosity of aqueous phase was adjusted using a commercial viscosifier (Emkarox®) which ensured Newtonian behavior to the continuous phase. The influences of two variables (rotational speed and oil volume fraction) on the kinetics of droplet fragmentation were examined in detail. Rotational speed was varied between 50 and 100 rad s-1 and had a strong effect on the kinetics of emulsion formation which was discussed on the basis of droplet fragmentation by shear forces. On the contrary, oil volume fraction (between 20 and 60% v/v) showed no marked effect, which appeared as an important result for scale-up considerations. A theoretical description of fragmentation mechanism was proposed and experimental results were compared to calculated values. © 2014 American Institute of Chemical Engineers.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The kinetics of mechanical emulsification in vane geometry was investigated using an original rheo-optic device. Hexadecane-in-water micronic emulsions were prepared using a nonionic polymeric surfactant (Brij700®) as stabilizer. The viscosity of aqueous phase was adjusted using a commercial viscosifier (Emkarox®) which ensured Newtonian behavior to the continuous phase. The influences of two variables (rotational speed and oil volume fraction) on the kinetics of droplet fragmentation were examined in detail. Rotational speed was varied between 50 and 100 rad s-1 and had a strong effect on the kinetics of emulsion formation which was discussed on the basis of droplet fragmentation by shear forces. On the contrary, oil volume fraction (between 20 and 60% v/v) showed no marked effect, which appeared as an important result for scale-up considerations. A theoretical description of fragmentation mechanism was proposed and experimental results were compared to calculated values. © 2014 American Institute of Chemical Engineers. |
Kinetics of formation of polysaccharide-covered micrometric oil droplets under mechanical agitation Article de journal R Covis; C Baravian; E Marie; A Durand Colloids and Surfaces A: Physicochemical and Engineering Aspects, 466 , p. 92–99, 2015. @article{Covis:2015, title = {Kinetics of formation of polysaccharide-covered micrometric oil droplets under mechanical agitation}, author = {R Covis and C Baravian and E Marie and A Durand}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84911906507&doi=10.1016%2fj.colsurfa.2014.10.048&partnerID=40&md5=f50de2bdc2c8f7afca00a18b12f62cea}, doi = {10.1016/j.colsurfa.2014.10.048}, year = {2015}, date = {2015-01-01}, journal = {Colloids and Surfaces A: Physicochemical and Engineering Aspects}, volume = {466}, pages = {92--99}, abstract = {Hexadecane-in-water emulsions were prepared by mechanical agitation in vane geometry using amphiphilic derivatives of dextran (nonionic bacterial polysaccharide) as stabilizers. Thanks to a laboratory-made experimental device coupling rheometry and back-scattered light analysis, we investigated the kinetics of droplet formation and the simultaneous variation of global shear stress. Oil volume fraction was varied between 10 and 50% and rotation speed between 40 and 100rads-1. The viscosity of aqueous phase was controlled by addition of sodium alginate and thus exhibited non-Newtonian rheological behavior. After forming a coarse emulsion, droplet rupture led to emulsions with volume-average droplet radius between 5 and 10μm provided that rotation speed and aqueous phase viscosity were high enough. Monitoring was consistent with droplet rupture occurring under shear forces such that capillary number remained approximately constant and close to its critical value. Although rotational speed and sodium alginate concentration had strong effect on emulsification kinetics, oil volume fraction had no significant effect. © 2014 Elsevier B.V.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Hexadecane-in-water emulsions were prepared by mechanical agitation in vane geometry using amphiphilic derivatives of dextran (nonionic bacterial polysaccharide) as stabilizers. Thanks to a laboratory-made experimental device coupling rheometry and back-scattered light analysis, we investigated the kinetics of droplet formation and the simultaneous variation of global shear stress. Oil volume fraction was varied between 10 and 50% and rotation speed between 40 and 100rads-1. The viscosity of aqueous phase was controlled by addition of sodium alginate and thus exhibited non-Newtonian rheological behavior. After forming a coarse emulsion, droplet rupture led to emulsions with volume-average droplet radius between 5 and 10μm provided that rotation speed and aqueous phase viscosity were high enough. Monitoring was consistent with droplet rupture occurring under shear forces such that capillary number remained approximately constant and close to its critical value. Although rotational speed and sodium alginate concentration had strong effect on emulsification kinetics, oil volume fraction had no significant effect. © 2014 Elsevier B.V. |
2014 |
Amphiphilic Macromolecules on Cell Membranes: From Protective Layers to Controlled Permeabilization Article de journal E Marie; S Sagan; S Cribier; C Tribet Journal of Membrane Biology, 247 (9-10), p. 861–881, 2014. @article{Marie:2014, title = {Amphiphilic Macromolecules on Cell Membranes: From Protective Layers to Controlled Permeabilization}, author = {E Marie and S Sagan and S Cribier and C Tribet}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84910118231&doi=10.1007%2fs00232-014-9679-3&partnerID=40&md5=2902d0eb4d955e4adbccc4a486976725}, doi = {10.1007/s00232-014-9679-3}, year = {2014}, date = {2014-01-01}, journal = {Journal of Membrane Biology}, volume = {247}, number = {9-10}, pages = {861--881}, abstract = {Antimicrobial and cell-penetrating peptides have inspired developments of abiotic membrane-active polymers that can coat, penetrate, or break lipid bilayers in model systems. Application to cell cultures is more recent, but remarkable bioactivities are already reported. Synthetic polymer chains were tailored to achieve (i) high biocide efficiencies, and selectivity for bacteria (Gram-positive/Gram-negative or bacterial/mammalian membranes), (ii) stable and mild encapsulation of viable isolated cells to escape immune systems, (iii) pH-, temperature-, or light-triggered interaction with cells. This review illustrates these recent achievements highlighting the use of abiotic polymers, and compares the major structural determinants that control efficiency of polymers and peptides. Charge density, sp. of cationic and guanidinium side groups, and hydrophobicity (including polarity of stimuli-responsive moieties) guide the design of new copolymers for the handling of cell membranes. While polycationic chains are generally used as biocidal or hemolytic agents, anionic amphiphilic polymers, including Amphipols, are particularly prone to mild permeabilization and/or intracell delivery. © 2014, Springer Science+Business Media New York.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Antimicrobial and cell-penetrating peptides have inspired developments of abiotic membrane-active polymers that can coat, penetrate, or break lipid bilayers in model systems. Application to cell cultures is more recent, but remarkable bioactivities are already reported. Synthetic polymer chains were tailored to achieve (i) high biocide efficiencies, and selectivity for bacteria (Gram-positive/Gram-negative or bacterial/mammalian membranes), (ii) stable and mild encapsulation of viable isolated cells to escape immune systems, (iii) pH-, temperature-, or light-triggered interaction with cells. This review illustrates these recent achievements highlighting the use of abiotic polymers, and compares the major structural determinants that control efficiency of polymers and peptides. Charge density, sp. of cationic and guanidinium side groups, and hydrophobicity (including polarity of stimuli-responsive moieties) guide the design of new copolymers for the handling of cell membranes. While polycationic chains are generally used as biocidal or hemolytic agents, anionic amphiphilic polymers, including Amphipols, are particularly prone to mild permeabilization and/or intracell delivery. © 2014, Springer Science+Business Media New York. |
Photocontrolled ionization in the corona of rodlike assemblies of diblock copolymers Article de journal J Sun; L Jia; M Emond; M -H Li; E Marie; L Jullien; C Tribet Macromolecules, 47 (5), p. 1684–1692, 2014. @article{Sun:2014, title = {Photocontrolled ionization in the corona of rodlike assemblies of diblock copolymers}, author = {J Sun and L Jia and M Emond and M -H Li and E Marie and L Jullien and C Tribet}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84900623616&doi=10.1021%2fma402591y&partnerID=40&md5=096eeaa435f309dc54c178b236d1de7c}, doi = {10.1021/ma402591y}, year = {2014}, date = {2014-01-01}, journal = {Macromolecules}, volume = {47}, number = {5}, pages = {1684--1692}, abstract = {To remotely control ionization of polymer assemblies, we tailored amphiphilic diblock polyacrylates with varying hydrophilic and hydrophobic block lengths and containing pendant 2-hydroxyazobenzene photoswitchable groups in the hydrophilic block. Formation in water of rodlike polymer micelles was obtained upon hydrophobic assembly of the pendant cholesterol groups present in the hydrophobic block. Phototriggered variation of both pH and UV-vis spectral properties confirmed that hydroxylazobenzene moieties, gathered in the hydrophilic corona, underwent both isomerization and ionization upon exposure to UV light. Dispersions of rods can accordingly be ionized on demand. © 2014 American Chemical Society.}, keywords = {}, pubstate = {published}, tppubtype = {article} } To remotely control ionization of polymer assemblies, we tailored amphiphilic diblock polyacrylates with varying hydrophilic and hydrophobic block lengths and containing pendant 2-hydroxyazobenzene photoswitchable groups in the hydrophilic block. Formation in water of rodlike polymer micelles was obtained upon hydrophobic assembly of the pendant cholesterol groups present in the hydrophobic block. Phototriggered variation of both pH and UV-vis spectral properties confirmed that hydroxylazobenzene moieties, gathered in the hydrophilic corona, underwent both isomerization and ionization upon exposure to UV light. Dispersions of rods can accordingly be ionized on demand. © 2014 American Chemical Society. |
2013 |
Triggering Cell Adhesion, Migration or Shape Change with a Dynamic Surface Coating Article de journal S F M van Dongen; P Maiuri; E Marie; C Tribet; M Piel Advanced Materials, 25 (12), p. 1687-1691, 2013, ISSN: 0935-9648. @article{vanDongen:2013, title = {Triggering Cell Adhesion, Migration or Shape Change with a Dynamic Surface Coating}, author = {S F M {van Dongen} and P Maiuri and E Marie and C Tribet and M Piel}, doi = {10.1002/adma.201204474}, issn = {0935-9648}, year = {2013}, date = {2013-03-01}, journal = {Advanced Materials}, volume = {25}, number = {12}, pages = {1687-1691}, abstract = {There's an APP for that: cell-repellent APP (azido-[polylysine-g-PEG]) is used to create substrates for spatially controlled dynamic cell adhesion. The simple addition of a functional peptide to the culture medium rapidly triggers cell adhesion. This highly accessible yet powerful technique allows diverse applications, demonstrated through tissue motility assays, patterned coculturing and triggered cell shape change. [GRAPHICS] .}, keywords = {}, pubstate = {published}, tppubtype = {article} } There's an APP for that: cell-repellent APP (azido-[polylysine-g-PEG]) is used to create substrates for spatially controlled dynamic cell adhesion. The simple addition of a functional peptide to the culture medium rapidly triggers cell adhesion. This highly accessible yet powerful technique allows diverse applications, demonstrated through tissue motility assays, patterned coculturing and triggered cell shape change. [GRAPHICS] . |