Agathe ESPAGNE

Chargée de recherche CNRS – Maître de conférences attachée à l’ENS

ENS – Département de chimie
24 rue Lhomond, 75005 Paris

Email: agathe.espagne@ens.psl.eu
Phone: 0144322413
Office: E121

ORCID: 0000-0002-2333-0227 (link)

Research interests

  • Photochemical mechanisms in light-sensitive proteins and molecules: cis/trans photoisomerization, photoinduced electron and proton transfer…
  • Fluorescent proteins: photophysics, photochemistry, engineering, applications
  • Time-resolved spectroscopy: transient absorption, time-resolved fluorescence, femtosecond to millisecond timescales

Short bio

Agathe Espagne studied chemistry at ENS in Paris. She specialized in mechanistic photochemistry and femtosecond transient absorption spectroscopy as a graduate student in the group of Monique Martin, studying the cis/trans photoisomerization of photoactive protein PYP. She received her PhD degree in physical chemistry in 2005. She was then trained in femtosecond fluorescence spectroscopy in the group of Ahmed H. Zewail at Caltech, and in femtosecond IR spectroscopy in the group of Thomas Elsaesser at MBI Berlin. In 2006 she joined the group of Klaus Brettel at CEA-Saclay, where she investigated photoinduced electron transfer in flavoproteins with transient absorption spectroscopy on subnanosecond to millisecond timescales. She was recruited by CNRS as a permanent researcher in 2007 and focussed then her research on fluorescent proteins photophysics, photochemistry, engineering and applications. First member of LCP in Orsay, she moved to ENS in 2012.

Publications

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2023

Multiscale Transient Absorption Study of the Fluorescent Protein Dreiklang and Two Point Variants Provides Insight into Photoswitching and Nonproductive Reaction Pathways Article de journal

Emilie Renouard; Magdalena Nowinska; Fabien Lacombat; Pascal Plaza; Pavel Müller; Agathe Espagne

The Journal of Physical Chemistry Letters, 14 (28), p. 6477-6485, 2023.

Near-infrared co-illumination of fluorescent proteins reduces photobleaching and phototoxicity Article de journal

L Ludvikova; E Simon; M Deygas; T Panier; M A Plamont; J Ollion; A Tebo; M Piel; L Jullien; L Robert; T Le Saux; A Espagne

Nature Biotechnology, p. 12, 2023, ISSN: 1087-0156.

2021

Ultrafast photoreduction dynamics of a new class of CPD photolyases Article de journal

Fabien Lacombat; Agathe Espagne; Nadia Dozova; Pascal Plaza; Pavel Müller; Hans-Joachim Emmerich; Martin Saft; Lars-Oliver Essen

Photochemical & Photobiological Sciences, 20 (6), p. 733-746, 2021, ISSN: 1474-9092.

2020

Dynamic contrast for overcoming spectral interferences in fluorescence imaging Article de journal

R Chouket; A Pellissier-Tanon; A Lemarchand; A Espagne; T Le Saux; L Jullien

Journal of Physics-Photonics, 2 (3), p. 8, 2020, ISSN: 2515-7647.

Dynamic contrast with reversibly photoswitchable fluorescent labels for imaging living cells Article de journal

R Chouket; A Pellissier-Tanon; A Lemarchand; A Espagne; T Le Saux; L Jullien

Chemical Science, 11 (11), p. 2882-2887, 2020, ISSN: 2041-6520.

2019

Photosensitized oxidative addition to gold(i) enables alkynylative cyclization of o-alkylnylphenols with iodoalkynes Article de journal

Zhonghua Xia; Vincent Corcé; Fen Zhao; Cédric Przybylski; Agathe Espagne; Ludovic Jullien; Thomas Le Saux; Yves Gimbert; Héloïse Dossmann; Virginie Mouriès-Mansuy; Cyril Ollivier; Louis Fensterbank

Nature Chemistry, 11 (9), p. 797-805, 2019, ISSN: 1755-4349.

Photosensitized oxidative addition to gold(I) enables alkynylative cyclization of o-alkylnylphenols with iodoalkynes Article de journal

Z H Xia; V Corce; F Zhao; C Przybylski; A Espagne; L Jullien; T Le Saux; Y Gimbert; H Dossmann; V Mouries-Mansuy; C Ollivier; L Fensterbank

Nature Chemistry, 11 (9), p. 797-805, 2019, ISSN: 1755-4330.

Ultrafast Oxidation of a Tyrosine by Proton-Coupled Electron Transfer Promotes Light Activation of an Animal-like Cryptochrome Article de journal

Fabien Lacombat; Agathe Espagne; Nadia Dozova; Pascal Plaza; Pavel Müller; Klaus Brettel; Sophie Franz-Badur; Lars-Oliver Essen

Journal of the American Chemical Society, 141 (34), p. 13394-13409, 2019, ISSN: 0002-7863.

2018

Delocalized hole transport coupled to sub-ns tryptophanyl deprotonation promotes photoreduction of class II photolyases Article de journal

F Lacombat; A Espagne; N Dozova; P Plaza; E Ignatz; S Kiontke; L O Essen

Physical Chemistry Chemical Physics, 20 (39), p. 25446-25457, 2018, ISSN: 1463-9076.

Macroscale fluorescence imaging against autofluorescence under ambient light Article de journal

R Zhang; R Chouket; M -A Plamont; Z Kelemen; A Espagne; A G Tebo; A Gautier; L Gissot; J -D Faure; L Jullien; V Croquette; T Le Saux

Light: Science and Applications, 7 (1), 2018.

2017

Photoinduced Chromophore Hydration in the Fluorescent Protein Dreiklang Is Triggered by Ultrafast Excited-State Proton Transfer Coupled to a Low-Frequency Vibration Article de journal

F Lacombat; P Plaza; M -A Plamont; A Espagne

Journal of Physical Chemistry Letters, 8 (7), p. 1489–1495, 2017.

Ultrafast flavin photoreduction in an oxidized animal (6-4) photolyase through an unconventional tryptophan tetrad Article de journal

P R Martin; F Lacombat; A Espagne; N Dozova; P Plaza; J Yamamoto; P Müller; K Brettel; A De La Lande

Physical Chemistry Chemical Physics, 19 (36), p. 24493–24504, 2017.

2016

Ultrafast Dynamics of a Green Fluorescent Protein Chromophore Analogue: Competition between Excited-State Proton Transfer and Torsional Relaxation Article de journal

T Chatterjee; F Lacombat; D Yadav; M Mandal; P Plaza; A Espagne; P K Mandal

Journal of Physical Chemistry B, 120 (36), p. 9716–9722, 2016.

2015

Photoswitching Kinetics and Phase-Sensitive Detection Add Discriminative Dimensions for Selective Fluorescence Imaging Article de journal

Jérôme Querard; Tal-Zvi Markus; Marie-Aude Plamont; Carole Gauron; Pengcheng Wang; Agathe Espagne; Michel Volovitch; Sophie Vriz; Vincent Croquette; Arnaud Gautier; Thomas Le Saux; Ludovic Jullien

Angewandte Chemie International Edition, 54 (9), p. 2633-2637, 2015, ISSN: 1433-7851.

Real-time monitoring of chromophore isomerization and deprotonation during the photoactivation of the fluorescent protein Dronpa Article de journal

D Yadav; F Lacombat; N Dozova; F Rappaport; P Plaza; A Espagne

Journal of Physical Chemistry B, 119 (6), p. 2404–2414, 2015.

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