2018
|
Probing the in-air growth of large area of 3D functional structures into a 2D supramolecular nanoporous network Article de journal R Brisse; D Guianvarc'h; C Mansuy; S Sagan; D Kreher; L Sosa-Vargas; L Hamitouche; V Humblot; I Arfaoui; V Labet; C Paris; C Petit; A -J Attias Chemical Communications, 54 (72), p. 10068–10071, 2018. @article{Brisse:2018,
title = {Probing the in-air growth of large area of 3D functional structures into a 2D supramolecular nanoporous network},
author = {R Brisse and D Guianvarc'h and C Mansuy and S Sagan and D Kreher and L Sosa-Vargas and L Hamitouche and V Humblot and I Arfaoui and V Labet and C Paris and C Petit and A -J Attias},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053165096&doi=10.1039%2fC8CC06125D&partnerID=40&md5=0fc8e0f5c85451c72df0638b78a31224},
doi = {10.1039/C8CC06125D},
year = {2018},
date = {2018-01-01},
journal = {Chemical Communications},
volume = {54},
number = {72},
pages = {10068--10071},
abstract = {Surface-confined host-guest chemistry at the air/solid interface is used for trapping a functionalized 3D Zn-phthalocyanine complex into a 2D porous supramolecular template allowing the large area functionalization of an sp2-hybridized carbon-based substrate as evidenced by STM, resonance Raman spectroscopy, and water contact angle measurements. © The Royal Society of Chemistry.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Surface-confined host-guest chemistry at the air/solid interface is used for trapping a functionalized 3D Zn-phthalocyanine complex into a 2D porous supramolecular template allowing the large area functionalization of an sp2-hybridized carbon-based substrate as evidenced by STM, resonance Raman spectroscopy, and water contact angle measurements. © The Royal Society of Chemistry. |
2016
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How to unveil self-quenched fluorophores and subsequently map the subcellular distribution of exogenous peptides Article de journal J -M Swiecicki; F Thiebaut; M Di Pisa; S Gourdin -Bertin; J Tailhades; C Mansuy; F Burlina; S Chwetzoff; G Trugnan; G Chassaing; S Lavielle Scientific Reports, 6 , 2016. @article{Swiecicki:2016,
title = {How to unveil self-quenched fluorophores and subsequently map the subcellular distribution of exogenous peptides},
author = {J -M Swiecicki and F Thiebaut and M Di Pisa and S Gourdin -Bertin and J Tailhades and C Mansuy and F Burlina and S Chwetzoff and G Trugnan and G Chassaing and S Lavielle},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84957837107&doi=10.1038%2fsrep20237&partnerID=40&md5=3b6ebb5cc1852323b58fc293948a9914},
doi = {10.1038/srep20237},
year = {2016},
date = {2016-01-01},
journal = {Scientific Reports},
volume = {6},
abstract = {Confocal laser scanning microscopy (CLSM) is the most popular technique for mapping the subcellular distribution of a fluorescent molecule and is widely used to investigate the penetration properties of exogenous macromolecules, such as cell-penetrating peptides (CPPs), within cells. Despite the membrane-association propensity of all these CPPs, the signal of the fluorescently labeled CPPs did not colocalize with the plasma membrane. We studied the origin of this fluorescence extinction and the overall consequence on the interpretation of intracellular localizations from CLSM pictures. We demonstrated that this discrepancy originated from fluorescence self-quenching. The fluorescence was unveiled by a "dilution" protocol, i.e. by varying the ratio fluorescent/non-fluorescent CPP. This strategy allowed us to rank with confidence the subcellular distribution of several CPPs, contributing to the elucidation of the penetration mechanism. More generally, this study proposes a broadly applicable and reliable method to study the subcellular distribution of any fluorescently labeled molecules.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Confocal laser scanning microscopy (CLSM) is the most popular technique for mapping the subcellular distribution of a fluorescent molecule and is widely used to investigate the penetration properties of exogenous macromolecules, such as cell-penetrating peptides (CPPs), within cells. Despite the membrane-association propensity of all these CPPs, the signal of the fluorescently labeled CPPs did not colocalize with the plasma membrane. We studied the origin of this fluorescence extinction and the overall consequence on the interpretation of intracellular localizations from CLSM pictures. We demonstrated that this discrepancy originated from fluorescence self-quenching. The fluorescence was unveiled by a "dilution" protocol, i.e. by varying the ratio fluorescent/non-fluorescent CPP. This strategy allowed us to rank with confidence the subcellular distribution of several CPPs, contributing to the elucidation of the penetration mechanism. More generally, this study proposes a broadly applicable and reliable method to study the subcellular distribution of any fluorescently labeled molecules. |
2015
|
Antibody Recognition in Multiple Sclerosis and Rett Syndrome Using a Collection of Linear and Cyclic N -Glucosylated Antigenic Probes: Antibody Recognition in Multiple Sclerosis and Rett Syndrome Article de journal Feliciana Real Fernández; Margherita Di Pisa; Giada Rossi; Nicolas Auberger; Olivier Lequin; Maud Larregola; Amina Benchohra; Christelle Mansuy; Gerard Chassaing; Francesco Lolli; Joussef Hayek; Solange Lavielle; Paolo Rovero; Jean-Maurice Mallet; Anna Maria Papini Biopolymers, 104 (5), p. 560-576, 2015. @article{RealFernandez:2015,
title = {Antibody Recognition in Multiple Sclerosis and Rett Syndrome Using a Collection of Linear and Cyclic N -Glucosylated Antigenic Probes: Antibody Recognition in Multiple Sclerosis and Rett Syndrome},
author = {Feliciana Real Fern\'{a}ndez and Margherita Di Pisa and Giada Rossi and Nicolas Auberger and Olivier Lequin and Maud Larregola and Amina Benchohra and Christelle Mansuy and Gerard Chassaing and Francesco Lolli and Joussef Hayek and Solange Lavielle and Paolo Rovero and Jean-Maurice Mallet and Anna Maria Papini},
doi = {10.1002/bip.22677},
year = {2015},
date = {2015-04-01},
journal = {Biopolymers},
volume = {104},
number = {5},
pages = {560-576},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Three-electrode analytical and preparative electrochemistry in micro-volume hanging droplets Article de journal Ana Isabel Perez Jimenez; Lylian Challier; Margherita Di Pisa; Manon Guille-Collignon; Frédéric Lema^itre; Solange Lavielle; Christelle Mansuy; Christian Amatore; Eric Labbé; Olivier Buriez Electrochemistry Communications, 54 , p. 41–45, 2015. @article{jimenez2015three,
title = {Three-electrode analytical and preparative electrochemistry in micro-volume hanging droplets},
author = {Ana Isabel Perez Jimenez and Lylian Challier and Margherita Di Pisa and Manon Guille-Collignon and Fr\'{e}d\'{e}ric Lema{^i}tre and Solange Lavielle and Christelle Mansuy and Christian Amatore and Eric Labb\'{e} and Olivier Buriez},
year = {2015},
date = {2015-01-01},
journal = {Electrochemistry Communications},
volume = {54},
pages = {41--45},
publisher = {Elsevier},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Unsaturated acyl chains dramatically enhanced cellular uptake by direct translocation of a minimalist oligo-arginine lipopeptide Article de journal J -M Swiecicki; M Di Pisa; F Lippi; S Chwetzoff; C Mansuy; G Trugnan; G Chassaing; S Lavielle; F Burlina Chemical Communications, 51 (78), p. 14656–14659, 2015. @article{Swiecicki:2015,
title = {Unsaturated acyl chains dramatically enhanced cellular uptake by direct translocation of a minimalist oligo-arginine lipopeptide},
author = {J -M Swiecicki and M Di Pisa and F Lippi and S Chwetzoff and C Mansuy and G Trugnan and G Chassaing and S Lavielle and F Burlina},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84941928625&doi=10.1039%2fc5cc06116d&partnerID=40&md5=288261f987c6ebd4b9aa0b0ec89f2154},
doi = {10.1039/c5cc06116d},
year = {2015},
date = {2015-01-01},
journal = {Chemical Communications},
volume = {51},
number = {78},
pages = {14656--14659},
abstract = {The recurring issue with cell penetrating peptides is how to increase direct translocation vs. endocytosis, to avoid premature degradation. Acylation by a cis unsaturated chain (C22:6) of a short cationic peptide provides a new rational design to favour diffuse cytosolic and dense Golgi localisations. © The Royal Society of Chemistry 2015.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The recurring issue with cell penetrating peptides is how to increase direct translocation vs. endocytosis, to avoid premature degradation. Acylation by a cis unsaturated chain (C22:6) of a short cationic peptide provides a new rational design to favour diffuse cytosolic and dense Golgi localisations. © The Royal Society of Chemistry 2015. |
Three-electrode analytical and preparative electrochemistry in micro-volume hanging droplets Article de journal Ana Isabel Perez Jimenez; Lylian Challier; Margherita Di Pisa; Manon Guille-Collignon; Frédéric Lemaître; Solange Lavielle; Christelle Mansuy; Christian Amatore; Eric Labbé; Olivier Buriez Electrochemistry Communications, 54 (0), p. 41-45, 2015. @article{RID:0721150706473-46b,
title = {Three-electrode analytical and preparative electrochemistry in micro-volume hanging droplets},
author = {Ana Isabel Perez Jimenez and Lylian Challier and Margherita Di Pisa and Manon Guille-Collignon and Fr\'{e}d\'{e}ric Lema\^{i}tre and Solange Lavielle and Christelle Mansuy and Christian Amatore and Eric Labb\'{e} and Olivier Buriez},
year = {2015},
date = {2015-01-01},
journal = {Electrochemistry Communications},
volume = {54},
number = {0},
pages = {41-45},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Accumulation of cell-penetrating peptides in large unilamellar vesicles: A straightforward screening assay for investigating the internalization mechanism Article de journal J -M Swiecicki; M Di Pisa; F Burlina; P Lécorché; C Mansuy; G Chassaing; S Lavielle Biopolymers, 104 (5), p. 533–543, 2015. @article{Swiecicki:2015a,
title = {Accumulation of cell-penetrating peptides in large unilamellar vesicles: A straightforward screening assay for investigating the internalization mechanism},
author = {J -M Swiecicki and M Di Pisa and F Burlina and P L\'{e}corch\'{e} and C Mansuy and G Chassaing and S Lavielle},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84975261080&doi=10.1002%2fbip.22652&partnerID=40&md5=b7e09c0b307479f4671edad8a6ef121d},
doi = {10.1002/bip.22652},
year = {2015},
date = {2015-01-01},
journal = {Biopolymers},
volume = {104},
number = {5},
pages = {533--543},
abstract = {The internalization of cell-penetrating peptides (CPPs) into liposomes (large unilamellar vesicles, LUVs) was studied with a rapid and robust procedure based on the quenching of a small fluorescent probe, 7-nitrobenz-2-oxa-1,3-diazole (NBD). Quenching can be achieved by reduction with dithionite or by pH jump. LUVs with different compositions of phospholipids (PLs) were used to screen the efficacy of different CPPs. In order to "validate" the composition of the membrane models, a control cationic peptide, which does not enter eukaryotic cells, was included in the study. It was found that pure DOPG or DOPG within ternary mixtures with cholesterol are the most appropriate models for studying CPP translocation. An anionic lipid, such as DOPG, is required for the adsorption of the basic peptides on the surface of LUVs. In addition, it acts as transfer agent through the lipid bilayer. A fluid phase and/or the presence of phase defects also appear mandatory for the internalization to occur. The neutralization of charges within an inverted micelle demonstrated in the case of DOPG and also proposed for a ternary mixture of PLs might not be the only mechanism for the CPP translocation. Finally, it is shown that oleic acid facilitates the entry inside LUVs in gel phase of a series of cationic peptides including CPPs and also the negative control peptide PKCi. © 2015 Wiley Periodicals, Inc.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The internalization of cell-penetrating peptides (CPPs) into liposomes (large unilamellar vesicles, LUVs) was studied with a rapid and robust procedure based on the quenching of a small fluorescent probe, 7-nitrobenz-2-oxa-1,3-diazole (NBD). Quenching can be achieved by reduction with dithionite or by pH jump. LUVs with different compositions of phospholipids (PLs) were used to screen the efficacy of different CPPs. In order to "validate" the composition of the membrane models, a control cationic peptide, which does not enter eukaryotic cells, was included in the study. It was found that pure DOPG or DOPG within ternary mixtures with cholesterol are the most appropriate models for studying CPP translocation. An anionic lipid, such as DOPG, is required for the adsorption of the basic peptides on the surface of LUVs. In addition, it acts as transfer agent through the lipid bilayer. A fluid phase and/or the presence of phase defects also appear mandatory for the internalization to occur. The neutralization of charges within an inverted micelle demonstrated in the case of DOPG and also proposed for a ternary mixture of PLs might not be the only mechanism for the CPP translocation. Finally, it is shown that oleic acid facilitates the entry inside LUVs in gel phase of a series of cationic peptides including CPPs and also the negative control peptide PKCi. © 2015 Wiley Periodicals, Inc. |
2014
|
The Efficacies of cell-penetrating peptides in accumulating in large unilamellar vesicles depend on their ability to form inverted micelles Article de journal J -M Swiecicki; A Bartsch; J Tailhades; M Di Pisa; B Heller; G Chassaing; C Mansuy; F Burlina; S Lavielle ChemBioChem, 15 (6), p. 884–891, 2014. @article{Swiecicki:2014,
title = {The Efficacies of cell-penetrating peptides in accumulating in large unilamellar vesicles depend on their ability to form inverted micelles},
author = {J -M Swiecicki and A Bartsch and J Tailhades and M Di Pisa and B Heller and G Chassaing and C Mansuy and F Burlina and S Lavielle},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84898543428&doi=10.1002%2fcbic.201300742&partnerID=40&md5=8311e7ff65490dd62302ee8d8cb54e86},
doi = {10.1002/cbic.201300742},
year = {2014},
date = {2014-01-01},
journal = {ChemBioChem},
volume = {15},
number = {6},
pages = {884--891},
abstract = {In this study, the direct translocation of cell-penetrating peptides (CPPs) into large unilamellar vesicles (LUVs) was shown to be rapid for all the most commonly used CPPs. This translocation led within a few minutes to intravesicular accumulation up to 0.5 mM, with no need for a transbilayer potential. The accumulation of CPPs inside LUVs was found to depend on CPP sequence, CPP extravesicular concentration and phospholipid (PL) composition, either in binary or ternary mixtures of PLs. More interestingly, the role of anionic phospholipid flip-flopping in the translocation process was ascertained. CPPs enhanced the flipping of PLs, and the intravesicular CPP accumulation directly correlated with the amount of anionic PLs that had been transferred from the external to the internal leaflet of the LUV bilayer, thus demonstrating the transport of peptide/lipid complexes as inverted micelles. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In this study, the direct translocation of cell-penetrating peptides (CPPs) into large unilamellar vesicles (LUVs) was shown to be rapid for all the most commonly used CPPs. This translocation led within a few minutes to intravesicular accumulation up to 0.5 mM, with no need for a transbilayer potential. The accumulation of CPPs inside LUVs was found to depend on CPP sequence, CPP extravesicular concentration and phospholipid (PL) composition, either in binary or ternary mixtures of PLs. More interestingly, the role of anionic phospholipid flip-flopping in the translocation process was ascertained. CPPs enhanced the flipping of PLs, and the intravesicular CPP accumulation directly correlated with the amount of anionic PLs that had been transferred from the external to the internal leaflet of the LUV bilayer, thus demonstrating the transport of peptide/lipid complexes as inverted micelles. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
2013
|
Peptide-coated nanoparticles: Adsorption and desorption studies of cationic peptides on nanodiamonds Article de journal J -M Swiecicki; J Tailhades; E Lepeltier; G Chassaing; S Lavielle; C Mansuy Colloids and Surfaces A: Physicochemical and Engineering Aspects, 431 , p. 73–79, 2013. @article{Swiecicki:2013,
title = {Peptide-coated nanoparticles: Adsorption and desorption studies of cationic peptides on nanodiamonds},
author = {J -M Swiecicki and J Tailhades and E Lepeltier and G Chassaing and S Lavielle and C Mansuy},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878363538&doi=10.1016%2fj.colsurfa.2013.04.037&partnerID=40&md5=5dc6b2ab0e1acf49db97dcac5135e873},
doi = {10.1016/j.colsurfa.2013.04.037},
year = {2013},
date = {2013-01-01},
journal = {Colloids and Surfaces A: Physicochemical and Engineering Aspects},
volume = {431},
pages = {73--79},
abstract = {The functionalization of nanoparticle surfaces remains a major challenge for broader applications in biology. To study the physisorption of peptides on nanodiamonds (NDs), we developed reliable and facile methods: UV titration, MALDI-TOF mass spectrometry or fluorescence spectrophotometry. These readily applicable procedures allowed us to analyze the adsorption and desorption behaviors of different cationic peptides. A rough estimate led to propose that an average of three to four shells of amphiphilic peptides rapidly adsorbed on NDs surface: about half of the molecules being adsorbed with a high affinity, while the other half may be exchanged within a few minutes. If a photoactivatable amino acid was incorporated in the peptide sequence, the subsequent irradiation allowed similarly the coating of three to five shells of peptides on the NDs surface, but in that case peptides were not exchangeable at all, even after a few days. Stable cationic nanodiamonds may be obtained by simple physisorption of amphiphilic peptides, leading to nanoparticles with a positive zeta potential in the appropriate range for biological applications. © 2013 Elsevier B.V.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The functionalization of nanoparticle surfaces remains a major challenge for broader applications in biology. To study the physisorption of peptides on nanodiamonds (NDs), we developed reliable and facile methods: UV titration, MALDI-TOF mass spectrometry or fluorescence spectrophotometry. These readily applicable procedures allowed us to analyze the adsorption and desorption behaviors of different cationic peptides. A rough estimate led to propose that an average of three to four shells of amphiphilic peptides rapidly adsorbed on NDs surface: about half of the molecules being adsorbed with a high affinity, while the other half may be exchanged within a few minutes. If a photoactivatable amino acid was incorporated in the peptide sequence, the subsequent irradiation allowed similarly the coating of three to five shells of peptides on the NDs surface, but in that case peptides were not exchangeable at all, even after a few days. Stable cationic nanodiamonds may be obtained by simple physisorption of amphiphilic peptides, leading to nanoparticles with a positive zeta potential in the appropriate range for biological applications. © 2013 Elsevier B.V. |
2012
|
Electrochemistry of a Ferrocene-Grafted Cell-Penetrating Peptide Article de journal Pierluca Messina; Géraldine Hallais; Eric Labbé; Marie Béranger; Gérard Chassaing; Solange Lavielle; Christelle Mansuy; Olivier Buriez; Christian Amatore Electrochimica Acta, 80 , p. 180-186, 2012. @article{Messina:2012a,
title = {Electrochemistry of a Ferrocene-Grafted Cell-Penetrating Peptide},
author = {Pierluca Messina and G\'{e}raldine Hallais and Eric Labb\'{e} and Marie B\'{e}ranger and G\'{e}rard Chassaing and Solange Lavielle and Christelle Mansuy and Olivier Buriez and Christian Amatore},
doi = {10.1016/j.electacta.2012.06.119},
year = {2012},
date = {2012-04-01},
journal = {Electrochimica Acta},
volume = {80},
pages = {180-186},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Electrochemistry of a ferrocene-grafted cell-penetrating peptide Article de journal Pierluca Messina; Géraldine Hallais; Eric Labbé; Marie Béranger; Gérard Chassaing; Solange Lavielle; Christelle Mansuy; Olivier Buriez; Christian Amatore Electrochimica acta, 80 , p. 180–186, 2012. @article{messina2012electrochemistry,
title = {Electrochemistry of a ferrocene-grafted cell-penetrating peptide},
author = {Pierluca Messina and G\'{e}raldine Hallais and Eric Labb\'{e} and Marie B\'{e}ranger and G\'{e}rard Chassaing and Solange Lavielle and Christelle Mansuy and Olivier Buriez and Christian Amatore},
year = {2012},
date = {2012-01-01},
journal = {Electrochimica acta},
volume = {80},
pages = {180--186},
publisher = {Elsevier},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Electrochemical studies of cell penetrating peptide interactions with lipid membranes Article de journal Peter W D'Amore; Christelle Mansuy; Marie Beranger; Olivier Buriez; Eric Labbe; Pierluca Messina; Solange Lavielle; Gerard Chassaing Abstracts of Papers of the American Chemical Society, 243 , 2012. @article{RID:0226140251298-39,
title = {Electrochemical studies of cell penetrating peptide interactions with lipid membranes},
author = {Peter W D'Amore and Christelle Mansuy and Marie Beranger and Olivier Buriez and Eric Labbe and Pierluca Messina and Solange Lavielle and Gerard Chassaing},
year = {2012},
date = {2012-01-01},
journal = {Abstracts of Papers of the American Chemical Society},
volume = {243},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2011
|
Nanodiamond as a vector for siRNA delivery to Ewing sarcoma cells Article de journal A Alhaddad; M -P Adam; J Botsoa; G Dantelle; S Perruchas; T Gacoin; C Mansuy; S Lavielle; C Malvy; F Treussart; J -R Bertrand Small, 7 (21), p. 3087–3095, 2011. @article{Alhaddad:2011,
title = {Nanodiamond as a vector for siRNA delivery to Ewing sarcoma cells},
author = {A Alhaddad and M -P Adam and J Botsoa and G Dantelle and S Perruchas and T Gacoin and C Mansuy and S Lavielle and C Malvy and F Treussart and J -R Bertrand},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-80155151294&doi=10.1002%2fsmll.201101193&partnerID=40&md5=2cf65b5cb0918112146ff9faf4f6c390},
doi = {10.1002/smll.201101193},
year = {2011},
date = {2011-01-01},
journal = {Small},
volume = {7},
number = {21},
pages = {3087--3095},
abstract = {The ability of diamond nanoparticles (nanodiamonds, NDs) to deliver small interfering RNA (siRNA) into Ewing sarcoma cells is investigated with a view to the possibility of in-vivo anticancer nucleic-acid drug delivery. siRNA is adsorbed onto NDs that are coated with cationic polymer. Cell uptake of NDs is demonstrated by taking advantage of the NDs' intrinsic fluorescence from embedded color-center defects. Cell toxicity of these coated NDs is shown to be low. Consistent with the internalization efficacy, a specific inhibition of EWS/Fli-1 gene expression is shown at the mRNA and protein level by the ND-vectorized siRNA in a serum-containing medium. Embedded color-center defects are used to monitor intrinsic nanodiamond (ND) fluorescence less than 24 h after the release of their FITC-labeled small interfering RNA (siRNA) cargo into Ewing sarcoma cells. The siRNA is delivered after adsorption onto NDs that have been coated with a cationic polymer. The use of NDs as siRNA delivery vehicles points to the possibility of in-vivo anticancer nucleic-acid drug delivery. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The ability of diamond nanoparticles (nanodiamonds, NDs) to deliver small interfering RNA (siRNA) into Ewing sarcoma cells is investigated with a view to the possibility of in-vivo anticancer nucleic-acid drug delivery. siRNA is adsorbed onto NDs that are coated with cationic polymer. Cell uptake of NDs is demonstrated by taking advantage of the NDs' intrinsic fluorescence from embedded color-center defects. Cell toxicity of these coated NDs is shown to be low. Consistent with the internalization efficacy, a specific inhibition of EWS/Fli-1 gene expression is shown at the mRNA and protein level by the ND-vectorized siRNA in a serum-containing medium. Embedded color-center defects are used to monitor intrinsic nanodiamond (ND) fluorescence less than 24 h after the release of their FITC-labeled small interfering RNA (siRNA) cargo into Ewing sarcoma cells. The siRNA is delivered after adsorption onto NDs that have been coated with a cationic polymer. The use of NDs as siRNA delivery vehicles points to the possibility of in-vivo anticancer nucleic-acid drug delivery. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
2009
|
Characterization and Scintillation Properties of Sol–Gel Derived Lu2SiO5:Ln3+ (Ln=Ce, Eu and Tb) Powders Article de journal C Mansuy; C Dujardin; R Mahiou; J M Nedelec Optical Materials, 31 (9), p. 1334-1336, 2009. @article{Mansuy:2009,
title = {Characterization and Scintillation Properties of Sol\textendashGel Derived Lu2SiO5:Ln3+ (Ln=Ce, Eu and Tb) Powders},
author = {C Mansuy and C Dujardin and R Mahiou and J M Nedelec},
doi = {10.1016/j.optmat.2008.10.008},
year = {2009},
date = {2009-04-01},
journal = {Optical Materials},
volume = {31},
number = {9},
pages = {1334-1336},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2008
|
Peptide-Grafted Nanodiamonds: Preparation, Cytotoxicity and Uptake in Cells Article de journal Stéphanie Vial; Christelle Mansuy; Sandrine Sagan; Theano Irinopoulou; Fabienne Burlina; Jean-Paul Boudou; Gérard Chassaing; Solange Lavielle ChemBioChem, 9 (13), p. 2113-2119, 2008. @article{Vial:2008,
title = {Peptide-Grafted Nanodiamonds: Preparation, Cytotoxicity and Uptake in Cells},
author = {St\'{e}phanie Vial and Christelle Mansuy and Sandrine Sagan and Theano Irinopoulou and Fabienne Burlina and Jean-Paul Boudou and G\'{e}rard Chassaing and Solange Lavielle},
doi = {10.1002/cbic.200800247},
year = {2008},
date = {2008-04-01},
journal = {ChemBioChem},
volume = {9},
number = {13},
pages = {2113-2119},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Materials Doping through Sol–Gel Chemistry: A Little Something Can Make a Big Difference Article de journal J -M Nedelec; L Courtheoux; E Jallot; C Kinowski; J Lao; P Laquerriere; C Mansuy; G Renaudin; S Turrell Journal of Sol-Gel Science and Technology, 46 (3), p. 259-271, 2008. @article{Nedelec:2008,
title = {Materials Doping through Sol\textendashGel Chemistry: A Little Something Can Make a Big Difference},
author = {J -M Nedelec and L Courtheoux and E Jallot and C Kinowski and J Lao and P Laquerriere and C Mansuy and G Renaudin and S Turrell},
doi = {10.1007/s10971-007-1665-0},
year = {2008},
date = {2008-04-01},
journal = {Journal of Sol-Gel Science and Technology},
volume = {46},
number = {3},
pages = {259-271},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2007
|
Concentration Effect on the Scintillation Properties of Sol–Gel Derived LuBO3 Doped with Eu3+ and Tb3+ Article de journal C Mansuy; J M Nedelec; C Dujardin; R Mahiou Optical Materials, 29 (6), p. 697-702, 2007. @article{Mansuy:2007,
title = {Concentration Effect on the Scintillation Properties of Sol\textendashGel Derived LuBO3 Doped with Eu3+ and Tb3+},
author = {C Mansuy and J M Nedelec and C Dujardin and R Mahiou},
doi = {10.1016/j.optmat.2005.10.017},
year = {2007},
date = {2007-04-01},
journal = {Optical Materials},
volume = {29},
number = {6},
pages = {697-702},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2006
|
Scintillation of Sol-Gel Derived Lutetium Orthophosphate Doped with Rare Earth Ions Article de journal C Mansuy; J M Nedelec; C Dujardin; R Mahiou Journal of Sol-Gel Science and Technology, 38 (1), p. 97-105, 2006. @article{Mansuy:2006a,
title = {Scintillation of Sol-Gel Derived Lutetium Orthophosphate Doped with Rare Earth Ions},
author = {C Mansuy and J M Nedelec and C Dujardin and R Mahiou},
doi = {10.1007/s10971-005-5639-9},
year = {2006},
date = {2006-04-01},
journal = {Journal of Sol-Gel Science and Technology},
volume = {38},
number = {1},
pages = {97-105},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Characterization of Sol-Gel Derived Scintillating LuBO3 Films Doped with Rare Earth Ions Article de journal C Mansuy; E Tomasella; R Mahiou; L Gengembre; J Grimblot; J M Nedelec Thin Solid Films, 515 (2), p. 666-669, 2006. @article{Mansuy:2006,
title = {Characterization of Sol-Gel Derived Scintillating LuBO3 Films Doped with Rare Earth Ions},
author = {C Mansuy and E Tomasella and R Mahiou and L Gengembre and J Grimblot and J M Nedelec},
doi = {10.1016/j.tsf.2005.12.235},
year = {2006},
date = {2006-04-01},
journal = {Thin Solid Films},
volume = {515},
number = {2},
pages = {666-669},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2005
|
Structural and Scintillation Properties of Spray Coated Lutetium Borate Films Doped with Ce3+ and Eu3+ Article de journal G Chadeyron-Bertrand; D Boyer; C Dujardin; C Mansuy; R Mahiou Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 229 (2), p. 232-239, 2005. @article{Chadeyron-Bertrand:2005,
title = {Structural and Scintillation Properties of Spray Coated Lutetium Borate Films Doped with Ce3+ and Eu3+},
author = {G {Chadeyron-Bertrand} and D Boyer and C Dujardin and C Mansuy and R Mahiou},
doi = {10.1016/j.nimb.2004.11.029},
year = {2005},
date = {2005-04-01},
journal = {Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms},
volume = {229},
number = {2},
pages = {232-239},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Preferential Site Substitution in Sol-Gel Derived Eu3+ Doped Lu2SiO5: A Combined Study by X-Ray Absorption and Luminescence Spectroscopies Article de journal C Mansuy; F Leroux; R Mahiou; J M Nedelec Journal of Materials Chemistry, 15 (38), p. 4129-4129, 2005. @article{Mansuy:2005,
title = {Preferential Site Substitution in Sol-Gel Derived Eu3+ Doped Lu2SiO5: A Combined Study by X-Ray Absorption and Luminescence Spectroscopies},
author = {C Mansuy and F Leroux and R Mahiou and J M Nedelec},
doi = {10.1039/b504303d},
year = {2005},
date = {2005-04-01},
journal = {Journal of Materials Chemistry},
volume = {15},
number = {38},
pages = {4129-4129},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2004
|
LUMINIX : synth`ese, caractérisation et mise en forme de luminophores denses pour la tomographie X : application `a un syst`eme de réalité augmentée Article de journal C Mansuy; G Chadeyron; J M Nedelec; R Mahiou; C Dujardin; J Mugnier; C Leluyer; B Peuchot; A Tanguy ITBM-RBM, 25 (5), p. 246-249, 2004. @article{Mansuy:2004,
title = {LUMINIX : synth`ese, caract\'{e}risation et mise en forme de luminophores denses pour la tomographie X : application `a un syst`eme de r\'{e}alit\'{e} augment\'{e}e},
author = {C Mansuy and G Chadeyron and J M Nedelec and R Mahiou and C Dujardin and J Mugnier and C Leluyer and B Peuchot and A Tanguy},
doi = {10.1016/j.rbmret.2004.09.001},
year = {2004},
date = {2004-04-01},
journal = {ITBM-RBM},
volume = {25},
number = {5},
pages = {246-249},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Scintillation of Sol?Gel Derived Lutetium Orthoborate Doped with Ce3+ Ions Article de journal C Mansuy; J M Nedelec; C Dujardin; R Mahiou Journal of Sol-Gel Science and Technology, 32 (1-3), p. 253-258, 2004. @article{Mansuy:2004b,
title = {Scintillation of Sol?Gel Derived Lutetium Orthoborate Doped with Ce3+ Ions},
author = {C Mansuy and J M Nedelec and C Dujardin and R Mahiou},
doi = {10.1007/s10971-004-5797-1},
year = {2004},
date = {2004-04-01},
journal = {Journal of Sol-Gel Science and Technology},
volume = {32},
number = {1-3},
pages = {253-258},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Molecular Design of Inorganic Scintillators: From Alkoxides to Scintillating Materials Article de journal Christelle Mansuy; Jean-Marie Nedelec; Rachid Mahiou Journal of Materials Chemistry, 14 (21), p. 3274-3274, 2004. @article{Mansuy:2004a,
title = {Molecular Design of Inorganic Scintillators: From Alkoxides to Scintillating Materials},
author = {Christelle Mansuy and Jean-Marie Nedelec and Rachid Mahiou},
doi = {10.1039/b405402d},
year = {2004},
date = {2004-04-01},
journal = {Journal of Materials Chemistry},
volume = {14},
number = {21},
pages = {3274-3274},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2003
|
A New Sol-Gel Route to Lu 2 SiO 5 (LSO) Scintillator: Powders and Thin Films Article de journal C Mansuy; R Mahiou; J M Nedelec Chemistry of Materials, 15 (17), p. 3242-3244, 2003. @article{Mansuy:2003,
title = {A New Sol-Gel Route to Lu 2 SiO 5 (LSO) Scintillator: Powders and Thin Films},
author = {C Mansuy and R Mahiou and J M Nedelec},
doi = {10.1021/cm034412t},
year = {2003},
date = {2003-04-01},
journal = {Chemistry of Materials},
volume = {15},
number = {17},
pages = {3242-3244},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Sol–Gel Derived YPO4 and LuPO4 Phosphors, a Spectroscopic Study Article de journal J M Nedelec; C Mansuy; R Mahiou Journal of Molecular Structure, 651-653 , p. 165-170, 2003. @article{Nedelec:2003,
title = {Sol\textendashGel Derived YPO4 and LuPO4 Phosphors, a Spectroscopic Study},
author = {J M Nedelec and C Mansuy and R Mahiou},
doi = {10.1016/S0022-2860(03)00104-2},
year = {2003},
date = {2003-04-01},
journal = {Journal of Molecular Structure},
volume = {651-653},
pages = {165-170},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
