A split fluorescent reporter with rapid and reversible complementation

Interactions between proteins play an essential role in metabolic and signaling pathways, cellular processes and organismal systems. We report the development of splitFAST, a fluorescence complementation system for the visualization of transient protein-protein interactions in living cells. Engineered from the fluorogenic reporter FAST (Fluorescence-Activating and absorption-Shifting Tag), which specifically and reversibly binds fluorogenic hydroxybenzylidene rhodanine (HBR) analogs, splitFAST displays rapid and reversible complementation, allowing the real-time visualization of both the formation and the dissociation of a protein assembly.

 

 

Press release available :

Spying on protein-protein interactions with fluorescent chemical-genetic hybrids

(in french : Espionner les interactions protéine-protéine avec des hybrides chemogénétiques fluorescents)

 

References:
A split fluorescent reporter with rapid and reversible complementation
Alison G. Tebo and Arnaud Gautier*
PASTEUR, Département de chimie, École normale supérieure, PSL University, Sorbonne Université́, CNRS, 24 Rue Lhomond, 75005 Paris, France
Nature Communications 10, 2822 (2019) 
DOI : 10.1038/s41467-019-10855-0