We designed a comprehensive, semi-automated and quantitative phagocytic assay, in conjunction with two types of microparticles showing monodisperse or polydisperse size distribution. We show that size is not a limiting factor of phagocytosis, but that phagocytosis depends on the total amount of surface area a cell is able to produce.
Phagocytosis by macrophages represents a fundamental process that consists in the uptake of pathogenic or cellular targets. The study of the role of the size of objects in their phagocytosis has lead to contradictory results in the last decades. To address this question, we designed a comprehensive, semi-automated and quantitative phagocytic assay, in conjunction with two types of microparticles showing mono disperse or polydisperse size distribution. We show that size is not a limiting factor of phagocytosis, but that phagocytosis depends on the total amount of surface area a cell is able to produce.
Reference :
A multiparametric and high-throughput assay to quantify the influence of target size on phagocytosis
L. Montel, L. Pinon, and J. Fattaccioli
Biophys. J. (2019)
https://doi.org/10.1016/j.bpj.2019.06.021
A multiparametric and high-throughput assay to quantify the influence of target size on phagocytosis