2008
|
Electrochemical monitoring of single cell secretion: Vesicular exocytosis and oxidative stress Article de journal C Amatore; S Arbault; M Guille; F Lemaître Chemical Reviews, 108 (7), p. 2585–2621, 2008. @article{Amatore:2008a,
title = {Electrochemical monitoring of single cell secretion: Vesicular exocytosis and oxidative stress},
author = {C Amatore and S Arbault and M Guille and F Lema\^{i}tre},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-49049112285&doi=10.1021%2fcr068062g&partnerID=40&md5=ae6a20b01b9ae57f75d37389b0ebe3ec},
doi = {10.1021/cr068062g},
year = {2008},
date = {2008-01-01},
journal = {Chemical Reviews},
volume = {108},
number = {7},
pages = {2585--2621},
abstract = {Several important contributions of electroanalytical techniques over the past 20 years for investigating three major biological processes at the single cell level: vesicular exocytosis, oxidative stress, and nitric oxide metabolism in brain have been reported. It is evident that molecular electrochemistry at microelectrodes enhances the understanding of central processes of cellular biology including cellular metabolism either at a single cell stage or in living tissues. Since cells have highly variable metabolism even among single genetic lines, studies performed at the single cell level allow delineating precisely the extent and limits of these variabilities.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Several important contributions of electroanalytical techniques over the past 20 years for investigating three major biological processes at the single cell level: vesicular exocytosis, oxidative stress, and nitric oxide metabolism in brain have been reported. It is evident that molecular electrochemistry at microelectrodes enhances the understanding of central processes of cellular biology including cellular metabolism either at a single cell stage or in living tissues. Since cells have highly variable metabolism even among single genetic lines, studies performed at the single cell level allow delineating precisely the extent and limits of these variabilities. |
2007
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Relationship between amperometric pre-spike feet and secretion granule composition in Chromaffin cells: An overview Article de journal C Amatore; S Arbault; I Bonifas; M Guille; F Lemaître; Y Verchier Biophysical Chemistry, 129 (2-3), p. 181–189, 2007. @article{Amatore:2007n,
title = {Relationship between amperometric pre-spike feet and secretion granule composition in Chromaffin cells: An overview},
author = {C Amatore and S Arbault and I Bonifas and M Guille and F Lema\^{i}tre and Y Verchier},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-34547560678&doi=10.1016%2fj.bpc.2007.05.018&partnerID=40&md5=c92376d4195c4dfec6edfd9e5eb98173},
doi = {10.1016/j.bpc.2007.05.018},
year = {2007},
date = {2007-01-01},
journal = {Biophysical Chemistry},
volume = {129},
number = {2-3},
pages = {181--189},
abstract = {Amperometry is a simple and powerful technique to study exocytosis at the single cell level. By positioning and polarizing (at an appropriate potential at which the molecules released by the cell can be oxidized) a carbon fiber microelectrode at the top of the cell, each exocytotic event is detected as an amperometric spike. More particularly, a portion of these spikes has previously been shown to present a foot, i.e. a small pedestal of current that precedes the spike itself. Among the important number of works dealing with the monitoring of exocytosis by amperometry under different conditions, only a few studies focus on amperometric spikes with a foot. In this work, by coupling our previous and recent experiments on chromaffin cells (that release catecholamines after stimulation) with literature data, we bring more light on what an amperometric foot and particularly its features, represents. © 2007.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Amperometry is a simple and powerful technique to study exocytosis at the single cell level. By positioning and polarizing (at an appropriate potential at which the molecules released by the cell can be oxidized) a carbon fiber microelectrode at the top of the cell, each exocytotic event is detected as an amperometric spike. More particularly, a portion of these spikes has previously been shown to present a foot, i.e. a small pedestal of current that precedes the spike itself. Among the important number of works dealing with the monitoring of exocytosis by amperometry under different conditions, only a few studies focus on amperometric spikes with a foot. In this work, by coupling our previous and recent experiments on chromaffin cells (that release catecholamines after stimulation) with literature data, we bring more light on what an amperometric foot and particularly its features, represents. © 2007. |
The Nature and Efficiency of Neurotransmitter Exocytosis Also Depend on Physicochemical Parameters Article de journal Christian Amatore; Stephane Arbault; Manon Guille; Frederic Lemaitre Chemphyschem, 8 (11), p. 1597-1605, 2007, ISSN: 1439-4235. @article{RN21b,
title = {The Nature and Efficiency of Neurotransmitter Exocytosis Also Depend on Physicochemical Parameters},
author = {Christian Amatore and Stephane Arbault and Manon Guille and Frederic Lemaitre},
doi = {10.1002/cphc.200700225},
issn = {1439-4235},
year = {2007},
date = {2007-01-01},
journal = {Chemphyschem},
volume = {8},
number = {11},
pages = {1597-1605},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2006
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Assessment of the electrochemical behavior of two-dimensional networks of single-walled carbon nanotubes Article de journal Neil R Wilson; Manon Guille; Ioana Dumitrescu; Virginia R Fernandez; Nicola C Rudd; Cara G Williams; Patrick R Unwin; Julie V Macpherson Analytical Chemistry, 78 (19), p. 7006-7015, 2006, (Times Cited: 28). @article{,
title = {Assessment of the electrochemical behavior of two-dimensional networks of single-walled carbon nanotubes},
author = {Neil R Wilson and Manon Guille and Ioana Dumitrescu and Virginia R Fernandez and Nicola C Rudd and Cara G Williams and Patrick R Unwin and Julie V Macpherson},
year = {2006},
date = {2006-01-01},
journal = {Analytical Chemistry},
volume = {78},
number = {19},
pages = {7006-7015},
note = {Times Cited: 28},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Glutamatergic control of microvascular tone by distinct GABA neurons in the cerebellum Article de journal Armelle Rancillac; Jean Rossier; Manon Guille; Xin-Kang Tong; Helene Geoffroy; Christian Amatore; Stephane Arbault; Edith Hamel; Bruno Cauli Journal of Neuroscience, 26 (26), p. 6997-7006, 2006, (Times Cited: 79). @article{,
title = {Glutamatergic control of microvascular tone by distinct GABA neurons in the cerebellum},
author = {Armelle Rancillac and Jean Rossier and Manon Guille and Xin-Kang Tong and Helene Geoffroy and Christian Amatore and Stephane Arbault and Edith Hamel and Bruno Cauli},
year = {2006},
date = {2006-01-01},
journal = {Journal of Neuroscience},
volume = {26},
number = {26},
pages = {6997-7006},
note = {Times Cited: 79},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Modelling release of nitric oxide in a slice of rat's brain: describing stimulated functional hyperemia with diffusion-reaction equations Article de journal A I Oleinick; C Amatore; M Guille; S Arbault; O V Klymenko; I Svir Mathematical Medicine and Biology-a Journal of the Ima, 23 (1), p. 27-44, 2006, (Times Cited: 12). @article{,
title = {Modelling release of nitric oxide in a slice of rat's brain: describing stimulated functional hyperemia with diffusion-reaction equations},
author = {A I Oleinick and C Amatore and M Guille and S Arbault and O V Klymenko and I Svir},
year = {2006},
date = {2006-01-01},
journal = {Mathematical Medicine and Biology-a Journal of the Ima},
volume = {23},
number = {1},
pages = {27-44},
note = {Times Cited: 12},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Nitric oxide release during evoked neuronal activity in cerebellum slices: Detection with platinized carbon-fiber microelectrodes Article de journal C Amatore; S Arbault; Y Bouret; B Cauli; M Guille; A Rancillac; J Rossier ChemPhysChem, 7 (1), p. 181–187, 2006. @article{Amatore:2006e,
title = {Nitric oxide release during evoked neuronal activity in cerebellum slices: Detection with platinized carbon-fiber microelectrodes},
author = {C Amatore and S Arbault and Y Bouret and B Cauli and M Guille and A Rancillac and J Rossier},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-31144464236&doi=10.1002%2fcphc.200500202&partnerID=40&md5=98d8f71d47f6a9199678a764ed645e15},
doi = {10.1002/cphc.200500202},
year = {2006},
date = {2006-01-01},
journal = {ChemPhysChem},
volume = {7},
number = {1},
pages = {181--187},
abstract = {Nitric oxide is an important biological messenger that particularly induces the relaxation of smooth muscle cells surrounding vessels, and, hence, controls the flow of blood. This mechanism is essential for brain function, and its fine control, termed functional hyperemia, is supposed to be realized by certain neurons that may release bursts of NO•. The aim of the present study is to examine the advantages of platinized carbon-fiber microelectrodes (5-7 μm tip diameter) for the direct and in situ electrochemical detection of NO• released by neurons into ex vivo cerebellum slices. After establishing the different analytical properties of the platinized carbon-fiber microelectrodes in vitro on NO• solutions at 50 nM to 1 mM concentration, they were characterized using DEA-NONOate solutions that chemically decompose into NO•, and therefore mimic the measurement of transient variations of NO• concentration in biological samples. This validated the present approach, so that direct, in situ ex vivo measurements of nitric oxide released by neurons in a rat cerebellar slice are presented and discussed. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Nitric oxide is an important biological messenger that particularly induces the relaxation of smooth muscle cells surrounding vessels, and, hence, controls the flow of blood. This mechanism is essential for brain function, and its fine control, termed functional hyperemia, is supposed to be realized by certain neurons that may release bursts of NO•. The aim of the present study is to examine the advantages of platinized carbon-fiber microelectrodes (5-7 μm tip diameter) for the direct and in situ electrochemical detection of NO• released by neurons into ex vivo cerebellum slices. After establishing the different analytical properties of the platinized carbon-fiber microelectrodes in vitro on NO• solutions at 50 nM to 1 mM concentration, they were characterized using DEA-NONOate solutions that chemically decompose into NO•, and therefore mimic the measurement of transient variations of NO• concentration in biological samples. This validated the present approach, so that direct, in situ ex vivo measurements of nitric oxide released by neurons in a rat cerebellar slice are presented and discussed. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA. |
Regulation of exocytosis in chromaffin cells by Trans-insertion of lysophosphatidylcholine and arachidonic acid into the outer leaflet of the cell membrane Article de journal C Amatore; S Arbault; Y Bouret; M Guille; F Lemaître; Y Verchier ChemBioChem, 7 (12), p. 1998–2003, 2006. @article{Amatore:2006i,
title = {Regulation of exocytosis in chromaffin cells by Trans-insertion of lysophosphatidylcholine and arachidonic acid into the outer leaflet of the cell membrane},
author = {C Amatore and S Arbault and Y Bouret and M Guille and F Lema\^{i}tre and Y Verchier},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33845431788&doi=10.1002%2fcbic.200600194&partnerID=40&md5=ee09aa44f07bb791da49cab4b5ce936a},
doi = {10.1002/cbic.200600194},
year = {2006},
date = {2006-01-01},
journal = {ChemBioChem},
volume = {7},
number = {12},
pages = {1998--2003},
abstract = {Vesicular exocytosis is an important complex process in the communication between cells in organisms. It controls the release of chemical and biochemical messengers stored in an emitting cell. In this report, exocytosis is studied amperometrically (at carbon fiber ultramicroelectrodes) at adrenal chromaffin cells, which release catecholamines after appropriate stimulation, while testing the effects due to trans-insertion of two exogenous compounds (lysophosphatidylcholine (LPC) and arachidonic acid (AA)) on the kinetics of exocytotic events. Amperometric analyses showed that, under the present conditions (short incubation times and micromolar LPC or AA solutions), LPC favors catecholamine release (rate, event frequency, charge released) while AA disfavors the exocytotic processes. The observed kinetic features are rationalized quantitatively by considering a stalk model, for the fusion pore formation, and the physical constraints applied to the cell membrane by the presence of small fractions of LPC and AA diluted in its external leaflet (trans-insertion). We also observed that the detected amount of neurotransmitters in the presence of LPC was larger than under control conditions, while the opposite trend is observed with AA. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Vesicular exocytosis is an important complex process in the communication between cells in organisms. It controls the release of chemical and biochemical messengers stored in an emitting cell. In this report, exocytosis is studied amperometrically (at carbon fiber ultramicroelectrodes) at adrenal chromaffin cells, which release catecholamines after appropriate stimulation, while testing the effects due to trans-insertion of two exogenous compounds (lysophosphatidylcholine (LPC) and arachidonic acid (AA)) on the kinetics of exocytotic events. Amperometric analyses showed that, under the present conditions (short incubation times and micromolar LPC or AA solutions), LPC favors catecholamine release (rate, event frequency, charge released) while AA disfavors the exocytotic processes. The observed kinetic features are rationalized quantitatively by considering a stalk model, for the fusion pore formation, and the physical constraints applied to the cell membrane by the presence of small fractions of LPC and AA diluted in its external leaflet (trans-insertion). We also observed that the detected amount of neurotransmitters in the presence of LPC was larger than under control conditions, while the opposite trend is observed with AA. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. |
2003
|
Dynamics of full fusion during vesicular exocytotic events: Release of adrenaline by chromaffin cells Article de journal C Amatore; S Arbault; I Bonifas; Y Bouret; M Erard; M Guille ChemPhysChem, 4 (2), p. 147–154, 2003. @article{Amatore:2003d,
title = {Dynamics of full fusion during vesicular exocytotic events: Release of adrenaline by chromaffin cells},
author = {C Amatore and S Arbault and I Bonifas and Y Bouret and M Erard and M Guille},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037450055&doi=10.1002%2fcphc.200390024&partnerID=40&md5=fba834474dafda276b2a40772d6c8796},
doi = {10.1002/cphc.200390024},
year = {2003},
date = {2003-01-01},
journal = {ChemPhysChem},
volume = {4},
number = {2},
pages = {147--154},
abstract = {Vesicular exocytosis is important in the communication between cells in complex organisms. It controls the release of specific chemical or biochemical messengers stored in the emitting cell, which elicit a response upon detection by the target cells. Secretion of a messenger molecule (a neurotransmitter) was measured electrochemically, which allowed the quantification of cellular events and the validation of current physicochemical models. This model led us to formulate predictions about the occurrence and kinetics of vesicular exocytotic events based on the physicochemical meaning of its key parameters. These predictions were tested successfully through a series of experiments on chromaffin cells, involving changes of osmotic conditions, presence of trivalent ions and cholesterol-induced structuring of the cell plasmic membrane.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Vesicular exocytosis is important in the communication between cells in complex organisms. It controls the release of specific chemical or biochemical messengers stored in the emitting cell, which elicit a response upon detection by the target cells. Secretion of a messenger molecule (a neurotransmitter) was measured electrochemically, which allowed the quantification of cellular events and the validation of current physicochemical models. This model led us to formulate predictions about the occurrence and kinetics of vesicular exocytotic events based on the physicochemical meaning of its key parameters. These predictions were tested successfully through a series of experiments on chromaffin cells, involving changes of osmotic conditions, presence of trivalent ions and cholesterol-induced structuring of the cell plasmic membrane. |
