2016
|
Multi-chambers Microsystem for Simultaneous and Direct Electrochemical Detection of Reactive Oxygen and Nitrogen Species Released by Cell Populations Article de journal Y Li; A Meunier; R Fulcrand; C Sella; C Amatore; L Thouin; F Lemaître; M Guille-Collignon Electroanalysis, 28 (8), p. 1865–1872, 2016. @article{Li:2016a,
title = {Multi-chambers Microsystem for Simultaneous and Direct Electrochemical Detection of Reactive Oxygen and Nitrogen Species Released by Cell Populations},
author = {Y Li and A Meunier and R Fulcrand and C Sella and C Amatore and L Thouin and F Lema\^{i}tre and M Guille-Collignon},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84960146089&doi=10.1002%2felan.201501157&partnerID=40&md5=31cc094d742b25352edb1219bc260f3c},
doi = {10.1002/elan.201501157},
year = {2016},
date = {2016-01-01},
journal = {Electroanalysis},
volume = {28},
number = {8},
pages = {1865--1872},
abstract = {Electrochemical quantification of four reactive oxygen and nitrogen species (e.g., H2O2, ONOO−, NO and NO2 −) emitted from macrophages populations was completed using a four-chambers microchip. This microchip was fabricated from a glass substrate equipped with four sets of microband electrodes. Each set consisted of a platinum-black coated working electrode combined to an Ag/AgCl reference and a Pt counter electrodes. A cover made of polydimethylsiloxane was designed with four wells whose open bottoms fitted over each set. Loading, culture and stimulation of cells were conducted within each well. After stimulation of macrophages by a calcium ionophore, amperometric responses were then monitored using a multipotentiostat to detect one species per well simultaneously. The results showed good reproducibility and were quite consistent with averaged amperometric detections obtained from single cell measurements. This methodology allowed drastically diminishing the number of experiments necessary to reach a statistical significance from n=100\textendash160 experiments required for single cell detection compared to n=5 experiments to obtain the mean behavior of the cell population. This work demonstrated that this simple and versatile microchip could be used for quantification of species in oxidative stress investigations on cell populations as well as a complementary tool to compare behaviors between single cell and cell population for investigations involving many experimental variables. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Electrochemical quantification of four reactive oxygen and nitrogen species (e.g., H2O2, ONOO−, NO and NO2 −) emitted from macrophages populations was completed using a four-chambers microchip. This microchip was fabricated from a glass substrate equipped with four sets of microband electrodes. Each set consisted of a platinum-black coated working electrode combined to an Ag/AgCl reference and a Pt counter electrodes. A cover made of polydimethylsiloxane was designed with four wells whose open bottoms fitted over each set. Loading, culture and stimulation of cells were conducted within each well. After stimulation of macrophages by a calcium ionophore, amperometric responses were then monitored using a multipotentiostat to detect one species per well simultaneously. The results showed good reproducibility and were quite consistent with averaged amperometric detections obtained from single cell measurements. This methodology allowed drastically diminishing the number of experiments necessary to reach a statistical significance from n=100–160 experiments required for single cell detection compared to n=5 experiments to obtain the mean behavior of the cell population. This work demonstrated that this simple and versatile microchip could be used for quantification of species in oxidative stress investigations on cell populations as well as a complementary tool to compare behaviors between single cell and cell population for investigations involving many experimental variables. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim |
Real Time Monitoring of Peroxynitrite by Stimulation of Macrophages with Ultramicroelectrodes Book Chapter Christian Amatore; Manon Guille-Collignon; Frederic Lemaitre Peteu, S F; Szunerits, S; Bayachou, M (Ed.): Peroxynitrite Detection in Biological Media: Challenges and Advances, 7 , p. 96-120, 2016, (Times Cited: 0). @inbook{,
title = {Real Time Monitoring of Peroxynitrite by Stimulation of Macrophages with Ultramicroelectrodes},
author = {Christian Amatore and Manon Guille-Collignon and Frederic Lemaitre},
editor = {S F Peteu and S Szunerits and M Bayachou},
year = {2016},
date = {2016-01-01},
booktitle = {Peroxynitrite Detection in Biological Media: Challenges and Advances},
volume = {7},
pages = {96-120},
series = {RSC Detection Science Series},
note = {Times Cited: 0},
keywords = {},
pubstate = {published},
tppubtype = {inbook}
}
|
2015
|
Bioanalytical applications of the fluorescence-electrochemistry combination Article de journal F Lemaître; M Guille-Collignon Actualite Chimique, (400-401), p. 17–19, 2015. @article{Lemaitre:2015,
title = {Bioanalytical applications of the fluorescence-electrochemistry combination},
author = {F Lema\^{i}tre and M Guille-Collignon},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84973499998&partnerID=40&md5=d5fb99aaf2d3336a53a286546cb86bdb},
year = {2015},
date = {2015-01-01},
journal = {Actualite Chimique},
number = {400-401},
pages = {17--19},
abstract = {Both fluorescence and electrochemistry techniques aim at converting a chemical signal into an optical or an electrical one respectively. Particularly, they correspond to appropriate techniques for investigating biological phenomena due to the electroactivity of many biomolecules while cells or proteins can be labeled with fluorophores. Therefore, this article is a non exhaustive presentation of the coupling between electrochemistry and fluorescence for biological investigations. By focusing on exocytosis, it also raises the question of the implementation of such a combination.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Both fluorescence and electrochemistry techniques aim at converting a chemical signal into an optical or an electrical one respectively. Particularly, they correspond to appropriate techniques for investigating biological phenomena due to the electroactivity of many biomolecules while cells or proteins can be labeled with fluorophores. Therefore, this article is a non exhaustive presentation of the coupling between electrochemistry and fluorescence for biological investigations. By focusing on exocytosis, it also raises the question of the implementation of such a combination. |
Evaluation of photosynthetic electrons derivation by exogenous redox mediators Article de journal Guillaume Longatte; Han-Yi Fu; Olivier Buriez; Eric Labbé; Francis-André Wollman; Christian Amatore; Fabrice Rappaport; Manon Guille-Collignon; Frédéric Lema^itre Biophysical Chemistry, 205 , p. 1–8, 2015. @article{longatte2015evaluation,
title = {Evaluation of photosynthetic electrons derivation by exogenous redox mediators},
author = {Guillaume Longatte and Han-Yi Fu and Olivier Buriez and Eric Labb\'{e} and Francis-Andr\'{e} Wollman and Christian Amatore and Fabrice Rappaport and Manon Guille-Collignon and Fr\'{e}d\'{e}ric Lema{^i}tre},
year = {2015},
date = {2015-01-01},
journal = {Biophysical Chemistry},
volume = {205},
pages = {1--8},
publisher = {Elsevier},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Evaluation of photosynthetic electrons derivation by exogenous redox mediators Article de journal Guillaume Longatte; Han-Yi Fu; Olivier Buriez; Eric Labbé; Francis-André Wollman; Christian Amatore; Fabrice Rappaport; Manon Guille-Collignon; Frédéric Lemaître Biophysical Chemistry, 205 , p. 1-8, 2015. @article{RID:0721150706473-48b,
title = {Evaluation of photosynthetic electrons derivation by exogenous redox mediators},
author = {Guillaume Longatte and Han-Yi Fu and Olivier Buriez and Eric Labb\'{e} and Francis-Andr\'{e} Wollman and Christian Amatore and Fabrice Rappaport and Manon Guille-Collignon and Fr\'{e}d\'{e}ric Lema\^{i}tre},
year = {2015},
date = {2015-01-01},
journal = {Biophysical Chemistry},
volume = {205},
pages = {1-8},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Three-electrode analytical and preparative electrochemistry in micro-volume hanging droplets Article de journal Ana Isabel Perez Jimenez; Lylian Challier; Margherita Di Pisa; Manon Guille-Collignon; Frédéric Lema^itre; Solange Lavielle; Christelle Mansuy; Christian Amatore; Eric Labbé; Olivier Buriez Electrochemistry Communications, 54 , p. 41–45, 2015. @article{jimenez2015three,
title = {Three-electrode analytical and preparative electrochemistry in micro-volume hanging droplets},
author = {Ana Isabel Perez Jimenez and Lylian Challier and Margherita Di Pisa and Manon Guille-Collignon and Fr\'{e}d\'{e}ric Lema{^i}tre and Solange Lavielle and Christelle Mansuy and Christian Amatore and Eric Labb\'{e} and Olivier Buriez},
year = {2015},
date = {2015-01-01},
journal = {Electrochemistry Communications},
volume = {54},
pages = {41--45},
publisher = {Elsevier},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Three-electrode analytical and preparative electrochemistry in micro-volume hanging droplets Article de journal Ana Isabel Perez Jimenez; Lylian Challier; Margherita Di Pisa; Manon Guille-Collignon; Frédéric Lemaître; Solange Lavielle; Christelle Mansuy; Christian Amatore; Eric Labbé; Olivier Buriez Electrochemistry Communications, 54 (0), p. 41-45, 2015. @article{RID:0721150706473-46b,
title = {Three-electrode analytical and preparative electrochemistry in micro-volume hanging droplets},
author = {Ana Isabel Perez Jimenez and Lylian Challier and Margherita Di Pisa and Manon Guille-Collignon and Fr\'{e}d\'{e}ric Lema\^{i}tre and Solange Lavielle and Christelle Mansuy and Christian Amatore and Eric Labb\'{e} and Olivier Buriez},
year = {2015},
date = {2015-01-01},
journal = {Electrochemistry Communications},
volume = {54},
number = {0},
pages = {41-45},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Vesicular Exocytosis and Microdevices - Microelectrode Arrays Article de journal Christian Amatore; Jerome Delacotte; Manon Guille-Collignon; Frederic Lemaitre Analyst, 140 (11), p. 3687-3695, 2015, ISSN: 0003-2654, (WOS:000354650300003). @article{Amatore:2015,
title = {Vesicular Exocytosis and Microdevices - Microelectrode Arrays},
author = {Christian Amatore and Jerome Delacotte and Manon {Guille-Collignon} and Frederic Lemaitre},
doi = {10.1039/c4an01932f},
issn = {0003-2654},
year = {2015},
date = {2015-01-01},
journal = {Analyst},
volume = {140},
number = {11},
pages = {3687-3695},
abstract = {Among all the analytical techniques capable of monitoring exocytosis in real time at the single cell level, electrochemistry (particularly amperometry at a constant potential) using ultramicroelectrodes has been demonstrated to be an important and convenient tool for more than two decades. Indeed, because the electrochemical sensor is located in the close vicinity of the emitting cell ("artificial synapse" configuration), much data can be gathered from the whole cell activity (secretion frequency) to the individual vesicular release (duration, fluxes or amount of molecules released) with an excellent sensitivity. However, such a single cell analysis and its intrinsic benefits are at the expense of the spatial resolution and/or the number of experiments. The quite recent development of microdevices/microsystems (and mainly the microelectrode arrays (MEAs)) offers in some way a complementary approach either by combining spectroscopy-microscopy or by implementing a multianalysis. Such developments are described and discussed in the present review over the 2005-2014 period.},
note = {WOS:000354650300003},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Among all the analytical techniques capable of monitoring exocytosis in real time at the single cell level, electrochemistry (particularly amperometry at a constant potential) using ultramicroelectrodes has been demonstrated to be an important and convenient tool for more than two decades. Indeed, because the electrochemical sensor is located in the close vicinity of the emitting cell ("artificial synapse" configuration), much data can be gathered from the whole cell activity (secretion frequency) to the individual vesicular release (duration, fluxes or amount of molecules released) with an excellent sensitivity. However, such a single cell analysis and its intrinsic benefits are at the expense of the spatial resolution and/or the number of experiments. The quite recent development of microdevices/microsystems (and mainly the microelectrode arrays (MEAs)) offers in some way a complementary approach either by combining spectroscopy-microscopy or by implementing a multianalysis. Such developments are described and discussed in the present review over the 2005-2014 period. |
2014
|
Amperometric Detection of Vesicular Exocytosis from BON Cells at Carbon Fiber Microelectrodes Article de journal Anne Meunier; Marine Bretou; Francois Darchen; Manon Guille Collignon; Frederic Lemaitre; Christian Amatore Electrochimica Acta, 126 , p. 74-80, 2014, ISSN: 0013-4686. @article{RN25b,
title = {Amperometric Detection of Vesicular Exocytosis from BON Cells at Carbon Fiber Microelectrodes},
author = {Anne Meunier and Marine Bretou and Francois Darchen and Manon Guille Collignon and Frederic Lemaitre and Christian Amatore},
doi = {10.1016/j.electacta.2013.07.110},
issn = {0013-4686},
year = {2014},
date = {2014-01-01},
journal = {Electrochimica Acta},
volume = {126},
pages = {74-80},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Anti-oxidant Mn-complexes: evaluation in cellular models of oxidative stress Article de journal Clotilde Policar; Anne-Sophie Bernard; Nicolas Delsuc; Geraldine Gazzah; Manon Guille; Frederic Lemaitre; Christian Amatore; Maria Bachelet; Joelle Masliah Journal of Biological Inorganic Chemistry, 19 , p. S739-S740, 2014, (Times Cited: 0
2
12th European Biological Inorganic Chemistry Conference (EuroBIC)
Aug 24-28, 2014
Zurich, SWITZERLAND
Univ Zurich). @article{,
title = {Anti-oxidant Mn-complexes: evaluation in cellular models of oxidative stress},
author = {Clotilde Policar and Anne-Sophie Bernard and Nicolas Delsuc and Geraldine Gazzah and Manon Guille and Frederic Lemaitre and Christian Amatore and Maria Bachelet and Joelle Masliah},
year = {2014},
date = {2014-01-01},
journal = {Journal of Biological Inorganic Chemistry},
volume = {19},
pages = {S739-S740},
note = {Times Cited: 0
2
12th European Biological Inorganic Chemistry Conference (EuroBIC)
Aug 24-28, 2014
Zurich, SWITZERLAND
Univ Zurich},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Electrochemical detection of nitric oxide and peroxynitrite anion in microchannels at highly sensitive platinum-black coated electrodes. application to ROS and RNS mixtures prior to biological investigations Article de journal Y Li; C Sella; F Lemaître; M Guille-Collignon; L Thouin; C Amatore Electrochimica Acta, 144 , p. 111–118, 2014. @article{Li:2014,
title = {Electrochemical detection of nitric oxide and peroxynitrite anion in microchannels at highly sensitive platinum-black coated electrodes. application to ROS and RNS mixtures prior to biological investigations},
author = {Y Li and C Sella and F Lema\^{i}tre and M Guille-Collignon and L Thouin and C Amatore},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84907101896&doi=10.1016%2fj.electacta.2014.08.046&partnerID=40&md5=2c011b35bc2ea90bd61dfef906786730},
doi = {10.1016/j.electacta.2014.08.046},
year = {2014},
date = {2014-01-01},
journal = {Electrochimica Acta},
volume = {144},
pages = {111--118},
abstract = {The electrochemical detection of nitric oxide (NO) and peroxynitrite anion (ONOO-) was investigated at Pt-black electrodes in microchannels. Owing to the high reactivity of these species under conditions close to physiological media, kinetic parameters were determined before evaluating the detection performances from synthetic solutions. Highly sensitive and stable Pt-black electrodes allowed detection limits down to 30 nM (NO) and 40 nM (ONOO-) to be reached with very high sensitivities. As NO and ONOO-are two relevant biological molecules involved in oxidative stress their simultaneous detections with other major molecules like hydrogen peroxide and nitrite were also performed and validated experimentally at pH 8.4. These results demonstrated that relative ROS/RNS contents in synthetic mixtures can be easily assessed at selected detection potentials. Beyond the interest of using small volumes in microfluidic channels, optimization of detection requires precise conditions easy to implement. These were delineated to lead in microdevices to high-performances detection of oxidative stress metabolites either from analytes or from the production of a few living cells like macrophages. © 2014 Elsevier Ltd.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The electrochemical detection of nitric oxide (NO) and peroxynitrite anion (ONOO-) was investigated at Pt-black electrodes in microchannels. Owing to the high reactivity of these species under conditions close to physiological media, kinetic parameters were determined before evaluating the detection performances from synthetic solutions. Highly sensitive and stable Pt-black electrodes allowed detection limits down to 30 nM (NO) and 40 nM (ONOO-) to be reached with very high sensitivities. As NO and ONOO-are two relevant biological molecules involved in oxidative stress their simultaneous detections with other major molecules like hydrogen peroxide and nitrite were also performed and validated experimentally at pH 8.4. These results demonstrated that relative ROS/RNS contents in synthetic mixtures can be easily assessed at selected detection potentials. Beyond the interest of using small volumes in microfluidic channels, optimization of detection requires precise conditions easy to implement. These were delineated to lead in microdevices to high-performances detection of oxidative stress metabolites either from analytes or from the production of a few living cells like macrophages. © 2014 Elsevier Ltd. |
Quantitative analyses of ROS and RNS production in breast cancer cell lines incubated with ferrocifens Article de journal C Lu; J -M Heldt; M Guille-Collignon; F Lemaître; G Jaouen; A Vessières; C Amatore ChemMedChem, 9 (6), p. 1286–1293, 2014. @article{Lu:2014,
title = {Quantitative analyses of ROS and RNS production in breast cancer cell lines incubated with ferrocifens},
author = {C Lu and J -M Heldt and M Guille-Collignon and F Lema\^{i}tre and G Jaouen and A Vessi\`{e}res and C Amatore},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84901310685&doi=10.1002%2fcmdc.201402016&partnerID=40&md5=ab2b01239f6a0d385b68c7964e868c67},
doi = {10.1002/cmdc.201402016},
year = {2014},
date = {2014-01-01},
journal = {ChemMedChem},
volume = {9},
number = {6},
pages = {1286--1293},
abstract = {Ferrocifens are an original class of ferrocifen-type breast cancer drugs. They possess anti-proliferative effects due to the association of the ferrocene moiety and the tamoxifen skeleton. In this work, fluorescence measurements indicated the production of reactive oxygen species (ROS) if hormone-dependent or -independent breast cancer cells were incubated with three hit ferrocifen compounds. Additionally, amperometry at ultramicroelectrodes was carried out to identify and quantify ROS and reactive nitrogen species (RNS) under stress conditions. Videomicroscopy was used to optimize the conditions employed for electrochemical investigations. Amperometry was then performed on two cell lines pre-incubated with each of the three ferrocifens. Interestingly, these results demonstrate that the presence of an aminoalkyl chain in the ferrocifen structure may confer a unique behavior toward both cell lines, in comparison with the two other compounds that lack this feature. Amped up! Ferrocifens are a promising class of bio-organometallic drugs against breast cancer. We investigated possible links between anti-proliferative activities coupled with amperometric detection and quantification of reactive oxygen and nitrogen species (ROS/RNS) from breast cancer cells incubated with various ferrocifen compounds. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Ferrocifens are an original class of ferrocifen-type breast cancer drugs. They possess anti-proliferative effects due to the association of the ferrocene moiety and the tamoxifen skeleton. In this work, fluorescence measurements indicated the production of reactive oxygen species (ROS) if hormone-dependent or -independent breast cancer cells were incubated with three hit ferrocifen compounds. Additionally, amperometry at ultramicroelectrodes was carried out to identify and quantify ROS and reactive nitrogen species (RNS) under stress conditions. Videomicroscopy was used to optimize the conditions employed for electrochemical investigations. Amperometry was then performed on two cell lines pre-incubated with each of the three ferrocifens. Interestingly, these results demonstrate that the presence of an aminoalkyl chain in the ferrocifen structure may confer a unique behavior toward both cell lines, in comparison with the two other compounds that lack this feature. Amped up! Ferrocifens are a promising class of bio-organometallic drugs against breast cancer. We investigated possible links between anti-proliferative activities coupled with amperometric detection and quantification of reactive oxygen and nitrogen species (ROS/RNS) from breast cancer cells incubated with various ferrocifen compounds. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Recent advances in Electrochemical Detection of Exocytosis Article de journal F Lemaître; M Guille-Collignon; C Amatore Electrochimica Acta, 140 , p. 457–466, 2014. @article{Lemaitre:2014,
title = {Recent advances in Electrochemical Detection of Exocytosis},
author = {F Lema\^{i}tre and M Guille-Collignon and C Amatore},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84906951395&doi=10.1016%2fj.electacta.2014.02.059&partnerID=40&md5=1344baf36cf42fb171ddb09dbf185ed5},
doi = {10.1016/j.electacta.2014.02.059},
year = {2014},
date = {2014-01-01},
journal = {Electrochimica Acta},
volume = {140},
pages = {457--466},
abstract = {Taking advantages of the analytical properties of ultramicroelectrodes, amperometry at constant potential has been a common technique for investigating exocytosis at the cell level for two decades. The historical experimental configuration, named "artificial synapse", mainly involves a micrometric carbon fiber electrode in the close vicinity of the investigated cell whose exocytotic release thus appears as a succession of amperometric spikes, whose frequency and shapes are particularly informative about the dynamics of the release process, while their areas (charge) directly correspond to the amount of molecules released. While the "single" carbon fiber still contributes to the understanding of the exocytotic mechanism, microsystems and microdevices have blossomed during the recent years and aim to gradually replace the historical experimental configuration by notably allowing coupling with spectroscopies and microscopies (optical, fluorescent). Such changes over the five last years are described and discussed in this review. © 2014 Elsevier Ltd.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Taking advantages of the analytical properties of ultramicroelectrodes, amperometry at constant potential has been a common technique for investigating exocytosis at the cell level for two decades. The historical experimental configuration, named "artificial synapse", mainly involves a micrometric carbon fiber electrode in the close vicinity of the investigated cell whose exocytotic release thus appears as a succession of amperometric spikes, whose frequency and shapes are particularly informative about the dynamics of the release process, while their areas (charge) directly correspond to the amount of molecules released. While the "single" carbon fiber still contributes to the understanding of the exocytotic mechanism, microsystems and microdevices have blossomed during the recent years and aim to gradually replace the historical experimental configuration by notably allowing coupling with spectroscopies and microscopies (optical, fluorescent). Such changes over the five last years are described and discussed in this review. © 2014 Elsevier Ltd. |
2013
|
Highly Sensitive Platinum-Black Coated Platinum Electrodes for Electrochemical Detection of Hydrogen Peroxide and Nitrite in Microchannel Article de journal Y Li; C Sella; F Lemaître; M Guille-Collignon; L Thouin; C Amatore Electroanalysis, 25 (4), p. 895–902, 2013. @article{Li:2013,
title = {Highly Sensitive Platinum-Black Coated Platinum Electrodes for Electrochemical Detection of Hydrogen Peroxide and Nitrite in Microchannel},
author = {Y Li and C Sella and F Lema\^{i}tre and M Guille-Collignon and L Thouin and C Amatore},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84876321602&doi=10.1002%2felan.201200456&partnerID=40&md5=46419ab05ae09fc2c4c12b913fad293d},
doi = {10.1002/elan.201200456},
year = {2013},
date = {2013-01-01},
journal = {Electroanalysis},
volume = {25},
number = {4},
pages = {895--902},
abstract = {The electrochemical detection of hydrogen peroxide and nitrite, as important representatives of cytotoxic reactive oxygen/nitrogen (ROS, RNS) species released during cellular oxidative bursts, is reported in microfluidic devices. Platinum-black coated platinum (Pt/Pt-black) electrodes were microfabricated and optimized to achieve optimal detection performances. In parallel, oxidation mechanisms of hydrogen peroxide and nitrite at these microelectrodes were investigated and electrode responses were compared to theoretical predictions based on convective mass transport at microchannel electrodes. In both cases, the active surface area of Pt/Pt-black electrodes allowed to avoid inhibition effect leading to long term stability in contrast to bare Pt electrodes. Such highly sensitive Pt/Pt-black electrodes allowed almost five decades of concentration range and detection limits down to 10nM, a range suitable for detection of ROS and RNS released by a few cells. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The electrochemical detection of hydrogen peroxide and nitrite, as important representatives of cytotoxic reactive oxygen/nitrogen (ROS, RNS) species released during cellular oxidative bursts, is reported in microfluidic devices. Platinum-black coated platinum (Pt/Pt-black) electrodes were microfabricated and optimized to achieve optimal detection performances. In parallel, oxidation mechanisms of hydrogen peroxide and nitrite at these microelectrodes were investigated and electrode responses were compared to theoretical predictions based on convective mass transport at microchannel electrodes. In both cases, the active surface area of Pt/Pt-black electrodes allowed to avoid inhibition effect leading to long term stability in contrast to bare Pt electrodes. Such highly sensitive Pt/Pt-black electrodes allowed almost five decades of concentration range and detection limits down to 10nM, a range suitable for detection of ROS and RNS released by a few cells. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Vesicular release of neurotransmitters: converting amperometric measurements into size, dynamics and energetics of initial fusion pores Article de journal Alexander Oleinick; Frederic Lemaitre; Manon Guille-Collignon; Irina Svir; Christian Amatore Faraday Discussions, 164 , p. 33-55, 2013, (Times Cited: 20). @article{,
title = {Vesicular release of neurotransmitters: converting amperometric measurements into size, dynamics and energetics of initial fusion pores},
author = {Alexander Oleinick and Frederic Lemaitre and Manon Guille-Collignon and Irina Svir and Christian Amatore},
year = {2013},
date = {2013-01-01},
journal = {Faraday Discussions},
volume = {164},
pages = {33-55},
note = {Times Cited: 20},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
