2014
|
Quantitative analyses of ROS and RNS production in breast cancer cell lines incubated with ferrocifens Article de journal C Lu; J -M Heldt; M Guille-Collignon; F Lemaître; G Jaouen; A Vessières; C Amatore ChemMedChem, 9 (6), p. 1286–1293, 2014. @article{Lu:2014,
title = {Quantitative analyses of ROS and RNS production in breast cancer cell lines incubated with ferrocifens},
author = {C Lu and J -M Heldt and M Guille-Collignon and F Lema\^{i}tre and G Jaouen and A Vessi\`{e}res and C Amatore},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84901310685&doi=10.1002%2fcmdc.201402016&partnerID=40&md5=ab2b01239f6a0d385b68c7964e868c67},
doi = {10.1002/cmdc.201402016},
year = {2014},
date = {2014-01-01},
journal = {ChemMedChem},
volume = {9},
number = {6},
pages = {1286--1293},
abstract = {Ferrocifens are an original class of ferrocifen-type breast cancer drugs. They possess anti-proliferative effects due to the association of the ferrocene moiety and the tamoxifen skeleton. In this work, fluorescence measurements indicated the production of reactive oxygen species (ROS) if hormone-dependent or -independent breast cancer cells were incubated with three hit ferrocifen compounds. Additionally, amperometry at ultramicroelectrodes was carried out to identify and quantify ROS and reactive nitrogen species (RNS) under stress conditions. Videomicroscopy was used to optimize the conditions employed for electrochemical investigations. Amperometry was then performed on two cell lines pre-incubated with each of the three ferrocifens. Interestingly, these results demonstrate that the presence of an aminoalkyl chain in the ferrocifen structure may confer a unique behavior toward both cell lines, in comparison with the two other compounds that lack this feature. Amped up! Ferrocifens are a promising class of bio-organometallic drugs against breast cancer. We investigated possible links between anti-proliferative activities coupled with amperometric detection and quantification of reactive oxygen and nitrogen species (ROS/RNS) from breast cancer cells incubated with various ferrocifen compounds. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Ferrocifens are an original class of ferrocifen-type breast cancer drugs. They possess anti-proliferative effects due to the association of the ferrocene moiety and the tamoxifen skeleton. In this work, fluorescence measurements indicated the production of reactive oxygen species (ROS) if hormone-dependent or -independent breast cancer cells were incubated with three hit ferrocifen compounds. Additionally, amperometry at ultramicroelectrodes was carried out to identify and quantify ROS and reactive nitrogen species (RNS) under stress conditions. Videomicroscopy was used to optimize the conditions employed for electrochemical investigations. Amperometry was then performed on two cell lines pre-incubated with each of the three ferrocifens. Interestingly, these results demonstrate that the presence of an aminoalkyl chain in the ferrocifen structure may confer a unique behavior toward both cell lines, in comparison with the two other compounds that lack this feature. Amped up! Ferrocifens are a promising class of bio-organometallic drugs against breast cancer. We investigated possible links between anti-proliferative activities coupled with amperometric detection and quantification of reactive oxygen and nitrogen species (ROS/RNS) from breast cancer cells incubated with various ferrocifen compounds. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Recent advances in Electrochemical Detection of Exocytosis Article de journal F Lemaître; M Guille-Collignon; C Amatore Electrochimica Acta, 140 , p. 457–466, 2014. @article{Lemaitre:2014,
title = {Recent advances in Electrochemical Detection of Exocytosis},
author = {F Lema\^{i}tre and M Guille-Collignon and C Amatore},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84906951395&doi=10.1016%2fj.electacta.2014.02.059&partnerID=40&md5=1344baf36cf42fb171ddb09dbf185ed5},
doi = {10.1016/j.electacta.2014.02.059},
year = {2014},
date = {2014-01-01},
journal = {Electrochimica Acta},
volume = {140},
pages = {457--466},
abstract = {Taking advantages of the analytical properties of ultramicroelectrodes, amperometry at constant potential has been a common technique for investigating exocytosis at the cell level for two decades. The historical experimental configuration, named "artificial synapse", mainly involves a micrometric carbon fiber electrode in the close vicinity of the investigated cell whose exocytotic release thus appears as a succession of amperometric spikes, whose frequency and shapes are particularly informative about the dynamics of the release process, while their areas (charge) directly correspond to the amount of molecules released. While the "single" carbon fiber still contributes to the understanding of the exocytotic mechanism, microsystems and microdevices have blossomed during the recent years and aim to gradually replace the historical experimental configuration by notably allowing coupling with spectroscopies and microscopies (optical, fluorescent). Such changes over the five last years are described and discussed in this review. © 2014 Elsevier Ltd.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Taking advantages of the analytical properties of ultramicroelectrodes, amperometry at constant potential has been a common technique for investigating exocytosis at the cell level for two decades. The historical experimental configuration, named "artificial synapse", mainly involves a micrometric carbon fiber electrode in the close vicinity of the investigated cell whose exocytotic release thus appears as a succession of amperometric spikes, whose frequency and shapes are particularly informative about the dynamics of the release process, while their areas (charge) directly correspond to the amount of molecules released. While the "single" carbon fiber still contributes to the understanding of the exocytotic mechanism, microsystems and microdevices have blossomed during the recent years and aim to gradually replace the historical experimental configuration by notably allowing coupling with spectroscopies and microscopies (optical, fluorescent). Such changes over the five last years are described and discussed in this review. © 2014 Elsevier Ltd. |
2013
|
Highly Sensitive Platinum-Black Coated Platinum Electrodes for Electrochemical Detection of Hydrogen Peroxide and Nitrite in Microchannel Article de journal Y Li; C Sella; F Lemaître; M Guille-Collignon; L Thouin; C Amatore Electroanalysis, 25 (4), p. 895–902, 2013. @article{Li:2013,
title = {Highly Sensitive Platinum-Black Coated Platinum Electrodes for Electrochemical Detection of Hydrogen Peroxide and Nitrite in Microchannel},
author = {Y Li and C Sella and F Lema\^{i}tre and M Guille-Collignon and L Thouin and C Amatore},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84876321602&doi=10.1002%2felan.201200456&partnerID=40&md5=46419ab05ae09fc2c4c12b913fad293d},
doi = {10.1002/elan.201200456},
year = {2013},
date = {2013-01-01},
journal = {Electroanalysis},
volume = {25},
number = {4},
pages = {895--902},
abstract = {The electrochemical detection of hydrogen peroxide and nitrite, as important representatives of cytotoxic reactive oxygen/nitrogen (ROS, RNS) species released during cellular oxidative bursts, is reported in microfluidic devices. Platinum-black coated platinum (Pt/Pt-black) electrodes were microfabricated and optimized to achieve optimal detection performances. In parallel, oxidation mechanisms of hydrogen peroxide and nitrite at these microelectrodes were investigated and electrode responses were compared to theoretical predictions based on convective mass transport at microchannel electrodes. In both cases, the active surface area of Pt/Pt-black electrodes allowed to avoid inhibition effect leading to long term stability in contrast to bare Pt electrodes. Such highly sensitive Pt/Pt-black electrodes allowed almost five decades of concentration range and detection limits down to 10nM, a range suitable for detection of ROS and RNS released by a few cells. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The electrochemical detection of hydrogen peroxide and nitrite, as important representatives of cytotoxic reactive oxygen/nitrogen (ROS, RNS) species released during cellular oxidative bursts, is reported in microfluidic devices. Platinum-black coated platinum (Pt/Pt-black) electrodes were microfabricated and optimized to achieve optimal detection performances. In parallel, oxidation mechanisms of hydrogen peroxide and nitrite at these microelectrodes were investigated and electrode responses were compared to theoretical predictions based on convective mass transport at microchannel electrodes. In both cases, the active surface area of Pt/Pt-black electrodes allowed to avoid inhibition effect leading to long term stability in contrast to bare Pt electrodes. Such highly sensitive Pt/Pt-black electrodes allowed almost five decades of concentration range and detection limits down to 10nM, a range suitable for detection of ROS and RNS released by a few cells. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Vesicular release of neurotransmitters: converting amperometric measurements into size, dynamics and energetics of initial fusion pores Article de journal Alexander Oleinick; Frederic Lemaitre; Manon Guille-Collignon; Irina Svir; Christian Amatore Faraday Discussions, 164 , p. 33-55, 2013, (Times Cited: 20). @article{,
title = {Vesicular release of neurotransmitters: converting amperometric measurements into size, dynamics and energetics of initial fusion pores},
author = {Alexander Oleinick and Frederic Lemaitre and Manon Guille-Collignon and Irina Svir and Christian Amatore},
year = {2013},
date = {2013-01-01},
journal = {Faraday Discussions},
volume = {164},
pages = {33-55},
note = {Times Cited: 20},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2012
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A New View of Electrochemistry at Highly Oriented Pyrolytic Graphite Article de journal Anisha N Patel; Manon Guille-Collignon; Michael A O'Connell; Wendy O Y Hung; Kim McKelvey; Julie V Macpherson; Patrick R Unwin Journal of the American Chemical Society, 134 (49), p. 20117-20130, 2012, (Times Cited: 157). @article{,
title = {A New View of Electrochemistry at Highly Oriented Pyrolytic Graphite},
author = {Anisha N Patel and Manon Guille-Collignon and Michael A O'Connell and Wendy O Y Hung and Kim McKelvey and Julie V Macpherson and Patrick R Unwin},
year = {2012},
date = {2012-01-01},
journal = {Journal of the American Chemical Society},
volume = {134},
number = {49},
pages = {20117-20130},
note = {Times Cited: 157},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Evaluation of the anti-oxidant properties of a SOD-mimic Mn-complex in activated macrophages Article de journal Anne-Sophie Bernard; Claire Giroud; Vincent H Y Ching; Anne Meunier; Vinita Ambike; Christian Amatore; Manon Guille-Collignon; Frederic Lemaitre; Clotilde Policar Dalton Transactions, 41 (21), p. 6399-6403, 2012, (Times Cited: 23). @article{,
title = {Evaluation of the anti-oxidant properties of a SOD-mimic Mn-complex in activated macrophages},
author = {Anne-Sophie Bernard and Claire Giroud and Vincent H Y Ching and Anne Meunier and Vinita Ambike and Christian Amatore and Manon Guille-Collignon and Frederic Lemaitre and Clotilde Policar},
year = {2012},
date = {2012-01-01},
journal = {Dalton Transactions},
volume = {41},
number = {21},
pages = {6399-6403},
note = {Times Cited: 23},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Indium Tin Oxide devices for amperometric detection of vesicular release by single cells Article de journal A Meunier; R Fulcrand; F Darchen; M Guille-Collignon; F Lemaître; C Amatore Biophysical Chemistry, 162 , p. 14–21, 2012. @article{Meunier:2012,
title = {Indium Tin Oxide devices for amperometric detection of vesicular release by single cells},
author = {A Meunier and R Fulcrand and F Darchen and M Guille-Collignon and F Lema\^{i}tre and C Amatore},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84857369437&doi=10.1016%2fj.bpc.2011.12.002&partnerID=40&md5=47662a922db0ab7f5679e2054ed78ffb},
doi = {10.1016/j.bpc.2011.12.002},
year = {2012},
date = {2012-01-01},
journal = {Biophysical Chemistry},
volume = {162},
pages = {14--21},
abstract = {The microfabrication and successful testing of a series of three ITO (Indium Tin Oxide) microsystems for amperometric detection of cells exocytosis are reported. These microdevices have been optimized in order to simultaneously (i) enhance signal-to-noise ratios, as required electrochemical monitoring, by defining appropriate electrodes geometry and size, and (ii) provide surface conditions which allow cells to be cultured over during one or two days, through apposite deposition of a collagen film. The intrinsic electrochemical quality of the microdevices as well as the effect of different collagen treatments were assessed by investigating the voltammetric responses of two classical redox systems, Ru(NH 3) 6 3+/2 + and Fe(CN) 6 3-/4 -. This established that a moderate collagen treatment does not incur any significant alteration of voltammetric responses or degradation of the excellent signal-to-noise ratio. Among these three microdevices, the most versatile one involved a configuration in which the ITO microelectrodes were delimited by a microchannel coiled into a spiral. Though providing extremely good electrochemical responses this specific design allowed proper seeding and culture of cells permitting either single cell or cell cluster stimulation and analysis. © 2011 Elsevier B.V. All rights reserved.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The microfabrication and successful testing of a series of three ITO (Indium Tin Oxide) microsystems for amperometric detection of cells exocytosis are reported. These microdevices have been optimized in order to simultaneously (i) enhance signal-to-noise ratios, as required electrochemical monitoring, by defining appropriate electrodes geometry and size, and (ii) provide surface conditions which allow cells to be cultured over during one or two days, through apposite deposition of a collagen film. The intrinsic electrochemical quality of the microdevices as well as the effect of different collagen treatments were assessed by investigating the voltammetric responses of two classical redox systems, Ru(NH 3) 6 3+/2 + and Fe(CN) 6 3-/4 -. This established that a moderate collagen treatment does not incur any significant alteration of voltammetric responses or degradation of the excellent signal-to-noise ratio. Among these three microdevices, the most versatile one involved a configuration in which the ITO microelectrodes were delimited by a microchannel coiled into a spiral. Though providing extremely good electrochemical responses this specific design allowed proper seeding and culture of cells permitting either single cell or cell cluster stimulation and analysis. © 2011 Elsevier B.V. All rights reserved. |
Nanoelectrodes for Determination of Reactive Oxygen and Nitrogen Species inside Murine Macrophages Article de journal Yixian Wang; Jean-Marc Noel; Jeyavel Velmurugan; Wojciech Nogala; Michael V Mirkin; Cong Lu; Manon Guille Collignon; Frederic Lemaitre; Christian Amatore Proceedings of the National Academy of Sciences of the United States of America, 109 (29), p. 11534-11539, 2012, ISSN: 0027-8424. @article{RN28b,
title = {Nanoelectrodes for Determination of Reactive Oxygen and Nitrogen Species inside Murine Macrophages},
author = {Yixian Wang and Jean-Marc Noel and Jeyavel Velmurugan and Wojciech Nogala and Michael V Mirkin and Cong Lu and Manon Guille Collignon and Frederic Lemaitre and Christian Amatore},
doi = {10.1073/pnas.1201552109},
issn = {0027-8424},
year = {2012},
date = {2012-01-01},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
volume = {109},
number = {29},
pages = {11534-11539},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2011
|
Artificial synapses and oxidative stress Article de journal C Amatore; S Arbault; M Guille; F Lemaître Actualite Chimique, (348-349), p. 25–31, 2011. @article{Amatore:2011a,
title = {Artificial synapses and oxidative stress},
author = {C Amatore and S Arbault and M Guille and F Lema\^{i}tre},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-80052988658&partnerID=40&md5=d58fc6ddbe08657329d14270cacd4b93},
year = {2011},
date = {2011-01-01},
journal = {Actualite Chimique},
number = {348-349},
pages = {25--31},
abstract = {Free radical production in aerobic living beings is generally perceived only through a negative viewpoint since one focuses mostly on their deleterious effects. Yet, oxidative stress is an essential mechanism underlying many important functions in aerobic organisms including non specific immunedefenses or many regulations. This is mostly true for the primary species of oxidative stress, namely the superoxide anion and nitric oxide. However,any direct investigation of the production of these primary species was hampered up to the introduction by our group of the .artificial synapse. in this context. This article describes in first instance the principle of the method and justifies its high analytical performance. It focuses then onto its application to the investigation of two central mechanisms relying on oxidative stress: phagocytosis, active in macrophages, which provides non-specific means of fighting against microbial infections, and neurovascular coupling in brain, which allows our neurons to regulate their blood supply asa function of their activity and which is at the very basis of the current imaging techniques of brain activity.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Free radical production in aerobic living beings is generally perceived only through a negative viewpoint since one focuses mostly on their deleterious effects. Yet, oxidative stress is an essential mechanism underlying many important functions in aerobic organisms including non specific immunedefenses or many regulations. This is mostly true for the primary species of oxidative stress, namely the superoxide anion and nitric oxide. However,any direct investigation of the production of these primary species was hampered up to the introduction by our group of the .artificial synapse. in this context. This article describes in first instance the principle of the method and justifies its high analytical performance. It focuses then onto its application to the investigation of two central mechanisms relying on oxidative stress: phagocytosis, active in macrophages, which provides non-specific means of fighting against microbial infections, and neurovascular coupling in brain, which allows our neurons to regulate their blood supply asa function of their activity and which is at the very basis of the current imaging techniques of brain activity. |
Coupling amperometry and total internal reflection fluorescence microscopy at ITO surfaces for monitoring exocytosis of single vesicles Article de journal A Meunier; O Jouannot; R Fulcrand; I Fanget; M Bretou; E Karatekin; S Arbault; M Guille; F Darchen; F Lemaître; C Amatore Angewandte Chemie - International Edition, 50 (22), p. 5081–5084, 2011. @article{Meunier:2011,
title = {Coupling amperometry and total internal reflection fluorescence microscopy at ITO surfaces for monitoring exocytosis of single vesicles},
author = {A Meunier and O Jouannot and R Fulcrand and I Fanget and M Bretou and E Karatekin and S Arbault and M Guille and F Darchen and F Lema\^{i}tre and C Amatore},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79956075610&doi=10.1002%2fanie.201101148&partnerID=40&md5=3b903d6f37bf9d7fde5edb97402f8f0d},
doi = {10.1002/anie.201101148},
year = {2011},
date = {2011-01-01},
journal = {Angewandte Chemie - International Edition},
volume = {50},
number = {22},
pages = {5081--5084},
abstract = {More transparency in bioanalysis: A microdevice based on transparent indium tin oxide (ITO) electrodes allows simultaneous total internal reflection fluorescence microscopy and amperometric measurements. Use of the device in the coupled optical and electrochemical detection of single exocytotic events is demonstrated with enterochromaffin BON cells (see picture). Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
More transparency in bioanalysis: A microdevice based on transparent indium tin oxide (ITO) electrodes allows simultaneous total internal reflection fluorescence microscopy and amperometric measurements. Use of the device in the coupled optical and electrochemical detection of single exocytotic events is demonstrated with enterochromaffin BON cells (see picture). Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
2010
|
In situ electrochemical monitoring of reactive oxygen and nitrogen species released by single MG63 osteosarcoma cell submitted to a mechanical stress Article de journal Ren Hu; Manon Guille; Stephane Arbault; Chang Jian Lin; Christian Amatore Physical Chemistry Chemical Physics, 12 (34), p. 10048-10054, 2010, (Times Cited: 15). @article{,
title = {In situ electrochemical monitoring of reactive oxygen and nitrogen species released by single MG63 osteosarcoma cell submitted to a mechanical stress},
author = {Ren Hu and Manon Guille and Stephane Arbault and Chang Jian Lin and Christian Amatore},
year = {2010},
date = {2010-01-01},
journal = {Physical Chemistry Chemical Physics},
volume = {12},
number = {34},
pages = {10048-10054},
note = {Times Cited: 15},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Prediction of Local pH Variations during amperometric monitoring of vesicular exocytotic events at chromaffin cells Article de journal C Amatore; S Arbault; Y Bouret; M Guille; F Lemaître ChemPhysChem, 11 (13), p. 2931–2941, 2010. @article{Amatore:2010c,
title = {Prediction of Local pH Variations during amperometric monitoring of vesicular exocytotic events at chromaffin cells},
author = {C Amatore and S Arbault and Y Bouret and M Guille and F Lema\^{i}tre},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77956858616&doi=10.1002%2fcphc.201000102&partnerID=40&md5=3e7bbeb9ad6306366c33ae3eebb2b086},
doi = {10.1002/cphc.201000102},
year = {2010},
date = {2010-01-01},
journal = {ChemPhysChem},
volume = {11},
number = {13},
pages = {2931--2941},
abstract = {Electrochemical monitoring of the exocytosis process is generally performed through amperometric oxidation of the electroactive messengers released by single living cells. Herein, we consider the vesicular release of catecholamines by chromaffin cells. Each exocytotic event is thus detected as a current spike whose morphology (intensity, duration, area, etc.) features the efficiency of the secretion process. However, the electrochemical oxidation of catechols produces quinone derivatives and protons. As a consequence, unless specific mechanisms may be adopted by a cell to regulate the pH near its membrane, the local pH between the cell membrane and the electrode necessarily drops within the electrode-cell cleft. Though this consequence of amperometric detection is generally ignored, it has been investigated in this work through simulation of the local pH drop created during the amperometric recording of a sequence of exocytotic events. This was performed based on frequencies and magnitudes of release detected at chromaffin cells. The corresponding acidification was shown to severely depend on the microelectrode radius. For usual 10 mm diameter carbon fiber electrodes, pH values below six were predicted to be reached within the electrode-cell cleft after monitoring a few current spikes. © 2010 Wiley-VCH Verlag GmbH& Co. KGaA, Weinheim.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Electrochemical monitoring of the exocytosis process is generally performed through amperometric oxidation of the electroactive messengers released by single living cells. Herein, we consider the vesicular release of catecholamines by chromaffin cells. Each exocytotic event is thus detected as a current spike whose morphology (intensity, duration, area, etc.) features the efficiency of the secretion process. However, the electrochemical oxidation of catechols produces quinone derivatives and protons. As a consequence, unless specific mechanisms may be adopted by a cell to regulate the pH near its membrane, the local pH between the cell membrane and the electrode necessarily drops within the electrode-cell cleft. Though this consequence of amperometric detection is generally ignored, it has been investigated in this work through simulation of the local pH drop created during the amperometric recording of a sequence of exocytotic events. This was performed based on frequencies and magnitudes of release detected at chromaffin cells. The corresponding acidification was shown to severely depend on the microelectrode radius. For usual 10 mm diameter carbon fiber electrodes, pH values below six were predicted to be reached within the electrode-cell cleft after monitoring a few current spikes. © 2010 Wiley-VCH Verlag GmbH& Co. KGaA, Weinheim. |
Striking Inflammation from Both Sides: Manganese(II) Pentaazamacrocyclic SOD Mimics Act Also as Nitric Oxide Dismutases: A Single-Cell Study Article de journal Milos R Filipovic; Alaric C W Koh; Stephane Arbault; Vesna Niketic; Andrea Debus; Ulrike Schleicher; Christian Bogdan; Manon Guille; Frederic Lemaitre; Christian Amatore; Ivana Ivanovic-Burmazovic Angewandte Chemie-International Edition, 49 (25), p. 4228-4232, 2010, ISSN: 1433-7851. @article{RN23b,
title = {Striking Inflammation from Both Sides: Manganese(II) Pentaazamacrocyclic SOD Mimics Act Also as Nitric Oxide Dismutases: A Single-Cell Study},
author = {Milos R Filipovic and Alaric C W Koh and Stephane Arbault and Vesna Niketic and Andrea Debus and Ulrike Schleicher and Christian Bogdan and Manon Guille and Frederic Lemaitre and Christian Amatore and Ivana {Ivanovic-Burmazovic}},
doi = {10.1002/anie.200905936},
issn = {1433-7851},
year = {2010},
date = {2010-01-01},
journal = {Angewandte Chemie-International Edition},
volume = {49},
number = {25},
pages = {4228-4232},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
2009
|
Invariance of exocytotic events detected by amperometry as a function of the carbon fiber microelectrode diameter Article de journal C Amatore; S Arbault; Y Bouret; M Guille; F Lemaître; Y Verchier Analytical Chemistry, 81 (8), p. 3087–3093, 2009. @article{Amatore:2009e,
title = {Invariance of exocytotic events detected by amperometry as a function of the carbon fiber microelectrode diameter},
author = {C Amatore and S Arbault and Y Bouret and M Guille and F Lema\^{i}tre and Y Verchier},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-65249125788&doi=10.1021%2fac900059s&partnerID=40&md5=3a9131b641fb9496ef04b7576ed24617},
doi = {10.1021/ac900059s},
year = {2009},
date = {2009-01-01},
journal = {Analytical Chemistry},
volume = {81},
number = {8},
pages = {3087--3093},
abstract = {Etched carbon fiber microelectrodes of different radii have been used for amperometric measurements of single exocytotic events occurring at adrenal chromaffin cells. Frequency, kinetic, and quantitative information on exo-cytosis provided by amperometric spikes were analyzed as a function of the surface area of the microelectrodes. Interestingly, the percentage of spikes with foot (as well as their own characteristics), a category revealing the existence of sufficient long-lasting fusion pores, was found to be constant whatever the microelectrode diameter was, whereas the probability of overlapping spikes decreased with the electrode size. This confirmed that the prespike foot could not feature accidental superimposition of separated events occurring at different places. Moreover, the features of amperometric spikes investigated here (charge, intensity and kinetics) were found constant for all microelectrode diameters. This demonstrated that the electrochemical measurement does not introduce significant bias onto the kinetics and thermodynamics of release during individual exocytotic events. All in all, this work evidences that information on exocytosis amperometri-cally recorded with the usual 7 μm diameter carbon fiber electrodes is biologically relevant, although the frequent overlap between spikes requires a censorship of the data during the analytical treatment. © 2009 American Chemical Society.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Etched carbon fiber microelectrodes of different radii have been used for amperometric measurements of single exocytotic events occurring at adrenal chromaffin cells. Frequency, kinetic, and quantitative information on exo-cytosis provided by amperometric spikes were analyzed as a function of the surface area of the microelectrodes. Interestingly, the percentage of spikes with foot (as well as their own characteristics), a category revealing the existence of sufficient long-lasting fusion pores, was found to be constant whatever the microelectrode diameter was, whereas the probability of overlapping spikes decreased with the electrode size. This confirmed that the prespike foot could not feature accidental superimposition of separated events occurring at different places. Moreover, the features of amperometric spikes investigated here (charge, intensity and kinetics) were found constant for all microelectrode diameters. This demonstrated that the electrochemical measurement does not introduce significant bias onto the kinetics and thermodynamics of release during individual exocytotic events. All in all, this work evidences that information on exocytosis amperometri-cally recorded with the usual 7 μm diameter carbon fiber electrodes is biologically relevant, although the frequent overlap between spikes requires a censorship of the data during the analytical treatment. © 2009 American Chemical Society. |
Quantitative investigations of amperometric spike feet suggest different controlling factors of the fusion pore in exocytosis at chromaffin cells Article de journal Christian Amatore; Stephane Arbault; Imelda Bonifas; Manon Guille Biophysical Chemistry, 143 (3), p. 124-131, 2009, (Times Cited: 25). @article{,
title = {Quantitative investigations of amperometric spike feet suggest different controlling factors of the fusion pore in exocytosis at chromaffin cells},
author = {Christian Amatore and Stephane Arbault and Imelda Bonifas and Manon Guille},
year = {2009},
date = {2009-01-01},
journal = {Biophysical Chemistry},
volume = {143},
number = {3},
pages = {124-131},
note = {Times Cited: 25},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
