2017
|
Electrochemical Harvesting of Photosynthetic Electrons from Unicellular Algae Population at the Preparative Scale by Using 2,6-dichlorobenzoquinone Article de journal G Longatte; F Rappaport; F -A Wollman; M Guille-Collignon; F Lemaître Electrochimica Acta, 236 , p. 337–342, 2017. @article{Longatte:2017,
title = {Electrochemical Harvesting of Photosynthetic Electrons from Unicellular Algae Population at the Preparative Scale by Using 2,6-dichlorobenzoquinone},
author = {G Longatte and F Rappaport and F -A Wollman and M Guille-Collignon and F Lema\^{i}tre},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85016504359&doi=10.1016%2fj.electacta.2017.03.124&partnerID=40&md5=d8a7614ce4f287a9f115d922ab5ee8f6},
doi = {10.1016/j.electacta.2017.03.124},
year = {2017},
date = {2017-01-01},
journal = {Electrochimica Acta},
volume = {236},
pages = {337--342},
abstract = {Oxygenic photosynthesis is the process used by plants, cyanobacteria or algae to convert the solar energy into a chemical one from the carbon dioxide reduction and water oxidation. In the past years, many strategies were implemented to take benefits from the overall low yield of this process to extract photosynthetic electrons and thus produce a sustainable photocurrent. In practice, electrochemical tools were involved and the principle of electrons harvestings was related to the step of electron transfer between the photosynthetic organism and a collecting electrode. In this context, works involving an algae population in suspension were rather scarce and rather focus on the grafting of the photosynthetic machinery at the electrode surface. Based on our previous works, we report here the implementation of an electrochemical set-up at the preparative scale to produce photocurrents. An algae suspension, i.e. an intact biological system to ensure culture and growth, was involved in presence of a centimeter-sized carbon gauze as the collecting electrode. The spectroelectrochemical cell contains 16 mL of suspension of a Chlamydomonas reinhardtii mutant with an appropriate mediator (2,6-DCBQ). Under these conditions, stable photocurrents were recorded over 1 h whose magnitude depends on the quinone concentration and the light illumination. © 2017 Elsevier Ltd},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Oxygenic photosynthesis is the process used by plants, cyanobacteria or algae to convert the solar energy into a chemical one from the carbon dioxide reduction and water oxidation. In the past years, many strategies were implemented to take benefits from the overall low yield of this process to extract photosynthetic electrons and thus produce a sustainable photocurrent. In practice, electrochemical tools were involved and the principle of electrons harvestings was related to the step of electron transfer between the photosynthetic organism and a collecting electrode. In this context, works involving an algae population in suspension were rather scarce and rather focus on the grafting of the photosynthetic machinery at the electrode surface. Based on our previous works, we report here the implementation of an electrochemical set-up at the preparative scale to produce photocurrents. An algae suspension, i.e. an intact biological system to ensure culture and growth, was involved in presence of a centimeter-sized carbon gauze as the collecting electrode. The spectroelectrochemical cell contains 16 mL of suspension of a Chlamydomonas reinhardtii mutant with an appropriate mediator (2,6-DCBQ). Under these conditions, stable photocurrents were recorded over 1 h whose magnitude depends on the quinone concentration and the light illumination. © 2017 Elsevier Ltd |
Indium Tin Oxide Microsystem for Electrochemical Detection of Exocytosis of Migratory Dendritic Cells Article de journal X Liu; M Bretou; A -M Lennon-Duménil; F Lemaître; M Guille-Collignon Electroanalysis, 29 (1), p. 197–202, 2017. @article{Liu:2017b,
title = {Indium Tin Oxide Microsystem for Electrochemical Detection of Exocytosis of Migratory Dendritic Cells},
author = {X Liu and M Bretou and A -M Lennon-Dum\'{e}nil and F Lema\^{i}tre and M Guille-Collignon},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84992456261&doi=10.1002%2felan.201600360&partnerID=40&md5=dbe0bcd2017bc2da52f267117aab382f},
doi = {10.1002/elan.201600360},
year = {2017},
date = {2017-01-01},
journal = {Electroanalysis},
volume = {29},
number = {1},
pages = {197--202},
abstract = {The design, fabrication and test of an indium tin oxide (ITO) microdevice to investigate exocytotic behaviors of migratory dendritic cells (DCs) in confined three-dimensional environment were reported in this work. Indeed, immature DCs were able to migrate into micro-fabricated biocompatible polydimethylsiloxane (PDMS) channels that mimic their natural constrained environment of tissues for patrolling in search of danger associated antigens through an endocytotic process called macropinocytosis. In order to coordinate membrane trafficking and prevent cell volume increment, DCs will release part of their contents back to the extracellular medium while migrating. Through electrochemical measurements, we demonstrated that exocytotic events of migratory DCs could be monitored by our ITO microdevice. In addition, the transparency of ITO electrode should facilitate future combining assays of exocytosis with other fluorescence-based measurements of cell physiology. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The design, fabrication and test of an indium tin oxide (ITO) microdevice to investigate exocytotic behaviors of migratory dendritic cells (DCs) in confined three-dimensional environment were reported in this work. Indeed, immature DCs were able to migrate into micro-fabricated biocompatible polydimethylsiloxane (PDMS) channels that mimic their natural constrained environment of tissues for patrolling in search of danger associated antigens through an endocytotic process called macropinocytosis. In order to coordinate membrane trafficking and prevent cell volume increment, DCs will release part of their contents back to the extracellular medium while migrating. Through electrochemical measurements, we demonstrated that exocytotic events of migratory DCs could be monitored by our ITO microdevice. In addition, the transparency of ITO electrode should facilitate future combining assays of exocytosis with other fluorescence-based measurements of cell physiology. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim |
Redesigning the QA binding site of Photosystem II allows reduction of exogenous quinones Article de journal H -Y Fu; D Picot; Y Choquet; G Longatte; A Sayegh; J Delacotte; M Guille-Collignon; F Lemaýtre; F Rappaport; F -A Wollman Nature Communications, 8 , 2017. @article{Fu:2017,
title = {Redesigning the QA binding site of Photosystem II allows reduction of exogenous quinones},
author = {H -Y Fu and D Picot and Y Choquet and G Longatte and A Sayegh and J Delacotte and M Guille-Collignon and F Lema\'{y}tre and F Rappaport and F -A Wollman},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85030692080&doi=10.1038%2fncomms15274&partnerID=40&md5=d602745399324d5a5eb6c25bd2e5a7c7},
doi = {10.1038/ncomms15274},
year = {2017},
date = {2017-01-01},
journal = {Nature Communications},
volume = {8},
abstract = {Strategies to harness photosynthesis from living organisms to generate electrical power have long been considered, yet efficiency remains low. Here, we aimed to reroute photosynthetic electron flow in photosynthetic organisms without compromising their phototrophic properties. We show that 2,6-dimethyl-p-benzoquinone (DMBQ) can be used as an electron mediator to assess the efficiency of mutations designed to engineer a novel electron donation pathway downstream of the primary electron acceptor QA of Photosystem (PS) II in the green alga Chlamydomonas reinhardtii. Through the use of structural prediction studies and a screen of site-directed PSII mutants we show that modifying the environment of the QA site increases the reduction rate of DMBQ. Truncating the C-terminus of the PsbT subunit protruding in the stroma provides evidence that shortening the distance between QA and DMBQ leads to sustained electron transfer to DMBQ, as confirmed by chronoamperometry, consistent with a bypass of the natural QA7circ; to QB pathway.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Strategies to harness photosynthesis from living organisms to generate electrical power have long been considered, yet efficiency remains low. Here, we aimed to reroute photosynthetic electron flow in photosynthetic organisms without compromising their phototrophic properties. We show that 2,6-dimethyl-p-benzoquinone (DMBQ) can be used as an electron mediator to assess the efficiency of mutations designed to engineer a novel electron donation pathway downstream of the primary electron acceptor QA of Photosystem (PS) II in the green alga Chlamydomonas reinhardtii. Through the use of structural prediction studies and a screen of site-directed PSII mutants we show that modifying the environment of the QA site increases the reduction rate of DMBQ. Truncating the C-terminus of the PsbT subunit protruding in the stroma provides evidence that shortening the distance between QA and DMBQ leads to sustained electron transfer to DMBQ, as confirmed by chronoamperometry, consistent with a bypass of the natural QA7circ; to QB pathway. |
2016
|
Astrocyte-derived adenosine is central to the hypnogenic effect of glucose Article de journal E Scharbarg; M Daenens; F Lemaître; H Geoffroy; M Guille-Collignon; T Gallopin; A Rancillac Scientific Reports, 6 , 2016. @article{Scharbarg:2016,
title = {Astrocyte-derived adenosine is central to the hypnogenic effect of glucose},
author = {E Scharbarg and M Daenens and F Lema\^{i}tre and H Geoffroy and M Guille-Collignon and T Gallopin and A Rancillac},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84954456013&doi=10.1038%2fsrep19107&partnerID=40&md5=e785b2e364698aba76cea9c2cade445e},
doi = {10.1038/srep19107},
year = {2016},
date = {2016-01-01},
journal = {Scientific Reports},
volume = {6},
abstract = {Sleep has been hypothesised to maintain a close relationship with metabolism. Here we focus on the brain structure that triggers slow-wave sleep, the ventrolateral preoptic nucleus (VLPO), to explore the cellular and molecular signalling pathways recruited by an increase in glucose concentration. We used infrared videomicroscopy on ex vivo brain slices to establish that glucose induces vasodilations specifically in the VLPO via the astrocytic release of adenosine. Real-time detection by in situ purine biosensors further revealed that the adenosine level doubles in response to glucose, and triples during the wakefulness period. Finally, patch-clamp recordings uncovered the depolarizing effect of adenosine and its A2A receptor agonist, CGS-21680, on sleep-promoting VLPO neurons. Altogether, our results provide new insights into the metabolically driven release of adenosine. We hypothesise that adenosine adjusts the local energy supply to local neuronal activity in response to glucose. This pathway could contribute to sleep-wake transition and sleep intensity.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Sleep has been hypothesised to maintain a close relationship with metabolism. Here we focus on the brain structure that triggers slow-wave sleep, the ventrolateral preoptic nucleus (VLPO), to explore the cellular and molecular signalling pathways recruited by an increase in glucose concentration. We used infrared videomicroscopy on ex vivo brain slices to establish that glucose induces vasodilations specifically in the VLPO via the astrocytic release of adenosine. Real-time detection by in situ purine biosensors further revealed that the adenosine level doubles in response to glucose, and triples during the wakefulness period. Finally, patch-clamp recordings uncovered the depolarizing effect of adenosine and its A2A receptor agonist, CGS-21680, on sleep-promoting VLPO neurons. Altogether, our results provide new insights into the metabolically driven release of adenosine. We hypothesise that adenosine adjusts the local energy supply to local neuronal activity in response to glucose. This pathway could contribute to sleep-wake transition and sleep intensity. |
Mechanism and analyses for extracting photosynthetic electrons using exogenous quinones-what makes a good extraction pathway? Article de journal G Longatte; F Rappaport; F -A Wollman; M Guille-Collignon; F Lemaître Photochemical and Photobiological Sciences, 15 (8), p. 969–979, 2016. @article{Longatte:2016,
title = {Mechanism and analyses for extracting photosynthetic electrons using exogenous quinones-what makes a good extraction pathway?},
author = {G Longatte and F Rappaport and F -A Wollman and M Guille-Collignon and F Lema\^{i}tre},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84982703184&doi=10.1039%2fc6pp00076b&partnerID=40&md5=bf03d01f7c5b19ff550d5b9f76d49eb9},
doi = {10.1039/c6pp00076b},
year = {2016},
date = {2016-01-01},
journal = {Photochemical and Photobiological Sciences},
volume = {15},
number = {8},
pages = {969--979},
abstract = {Plants or algae take many benefits from oxygenic photosynthesis by converting solar energy into chemical energy through the synthesis of carbohydrates from carbon dioxide and water. However, the overall yield of this process is rather low (about 4% of the total energy available from sunlight is converted into chemical energy). This is the principal reason why recently many studies have been devoted to extraction of photosynthetic electrons in order to produce a sustainable electric current. Practically, the electron transfer occurs between the photosynthetic organism and an electrode and can be assisted by an exogenous mediator, mainly a quinone. In this regard, we recently reported on a method involving fluorescence measurements to estimate the ability of different quinones to extract photosynthetic electrons from a mutant of Chlamydomonas reinhardtii. In the present work, we used the same kind of methodology to establish a zone diagram for predicting the most suitable experimental conditions to extract photoelectrons from intact algae (quinone concentration and light intensity) as a function of the purpose of the study. This will provide further insights into the extraction mechanism of photosynthetic electrons using exogenous quinones. Indeed fluorescence measurements allowed us to model the capacity of photosynthetic algae to donate electrons to an exogenous quinone by considering a numerical parameter called "open center ratio" which is related to the Photosystem II acceptor redox state. Then, using it as a proxy for investigating the extraction of photosynthetic electrons by means of an exogenous quinone, 2,6-DCBQ, we suggested an extraction mechanism that was globally found consistent with the experimentally extracted parameters. © The Royal Society of Chemistry and Owner Societies 2016.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Plants or algae take many benefits from oxygenic photosynthesis by converting solar energy into chemical energy through the synthesis of carbohydrates from carbon dioxide and water. However, the overall yield of this process is rather low (about 4% of the total energy available from sunlight is converted into chemical energy). This is the principal reason why recently many studies have been devoted to extraction of photosynthetic electrons in order to produce a sustainable electric current. Practically, the electron transfer occurs between the photosynthetic organism and an electrode and can be assisted by an exogenous mediator, mainly a quinone. In this regard, we recently reported on a method involving fluorescence measurements to estimate the ability of different quinones to extract photosynthetic electrons from a mutant of Chlamydomonas reinhardtii. In the present work, we used the same kind of methodology to establish a zone diagram for predicting the most suitable experimental conditions to extract photoelectrons from intact algae (quinone concentration and light intensity) as a function of the purpose of the study. This will provide further insights into the extraction mechanism of photosynthetic electrons using exogenous quinones. Indeed fluorescence measurements allowed us to model the capacity of photosynthetic algae to donate electrons to an exogenous quinone by considering a numerical parameter called "open center ratio" which is related to the Photosystem II acceptor redox state. Then, using it as a proxy for investigating the extraction of photosynthetic electrons by means of an exogenous quinone, 2,6-DCBQ, we suggested an extraction mechanism that was globally found consistent with the experimentally extracted parameters. © The Royal Society of Chemistry and Owner Societies 2016. |
More Transparency in BioAnalysis of Exocytosis: Coupling of Electrochemistry and Fluorescence Microscopy at ITO Electrodes Book Chapter Xiaoqing Liu; Damien Quinton; Lihui Hu; Christian Amatore; Jerome Delacotte; Frederic Lemaitre; Manon Guille-Collignon Raspaud, E; Marliere, C; Regeard, C; Cornut, R; MealletRenault, R (Ed.): Electro-Activity of Biological Systems, 6 , 2016, (Times Cited: 0
International and Multidisciplinary Workshop on Electro-Activity of Biological Systems (EABS)
Nov 18-19, 2015
Paris, FRANCE). @inbook{,
title = {More Transparency in BioAnalysis of Exocytosis: Coupling of Electrochemistry and Fluorescence Microscopy at ITO Electrodes},
author = {Xiaoqing Liu and Damien Quinton and Lihui Hu and Christian Amatore and Jerome Delacotte and Frederic Lemaitre and Manon Guille-Collignon},
editor = {E Raspaud and C Marliere and C Regeard and R Cornut and R MealletRenault},
year = {2016},
date = {2016-01-01},
booktitle = {Electro-Activity of Biological Systems},
volume = {6},
series = {BIO Web of Conferences},
note = {Times Cited: 0
International and Multidisciplinary Workshop on Electro-Activity of Biological Systems (EABS)
Nov 18-19, 2015
Paris, FRANCE},
keywords = {},
pubstate = {published},
tppubtype = {inbook}
}
|
Multi-chambers Microsystem for Simultaneous and Direct Electrochemical Detection of Reactive Oxygen and Nitrogen Species Released by Cell Populations Article de journal Y Li; A Meunier; R Fulcrand; C Sella; C Amatore; L Thouin; F Lemaître; M Guille-Collignon Electroanalysis, 28 (8), p. 1865–1872, 2016. @article{Li:2016a,
title = {Multi-chambers Microsystem for Simultaneous and Direct Electrochemical Detection of Reactive Oxygen and Nitrogen Species Released by Cell Populations},
author = {Y Li and A Meunier and R Fulcrand and C Sella and C Amatore and L Thouin and F Lema\^{i}tre and M Guille-Collignon},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84960146089&doi=10.1002%2felan.201501157&partnerID=40&md5=31cc094d742b25352edb1219bc260f3c},
doi = {10.1002/elan.201501157},
year = {2016},
date = {2016-01-01},
journal = {Electroanalysis},
volume = {28},
number = {8},
pages = {1865--1872},
abstract = {Electrochemical quantification of four reactive oxygen and nitrogen species (e.g., H2O2, ONOO−, NO and NO2 −) emitted from macrophages populations was completed using a four-chambers microchip. This microchip was fabricated from a glass substrate equipped with four sets of microband electrodes. Each set consisted of a platinum-black coated working electrode combined to an Ag/AgCl reference and a Pt counter electrodes. A cover made of polydimethylsiloxane was designed with four wells whose open bottoms fitted over each set. Loading, culture and stimulation of cells were conducted within each well. After stimulation of macrophages by a calcium ionophore, amperometric responses were then monitored using a multipotentiostat to detect one species per well simultaneously. The results showed good reproducibility and were quite consistent with averaged amperometric detections obtained from single cell measurements. This methodology allowed drastically diminishing the number of experiments necessary to reach a statistical significance from n=100\textendash160 experiments required for single cell detection compared to n=5 experiments to obtain the mean behavior of the cell population. This work demonstrated that this simple and versatile microchip could be used for quantification of species in oxidative stress investigations on cell populations as well as a complementary tool to compare behaviors between single cell and cell population for investigations involving many experimental variables. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Electrochemical quantification of four reactive oxygen and nitrogen species (e.g., H2O2, ONOO−, NO and NO2 −) emitted from macrophages populations was completed using a four-chambers microchip. This microchip was fabricated from a glass substrate equipped with four sets of microband electrodes. Each set consisted of a platinum-black coated working electrode combined to an Ag/AgCl reference and a Pt counter electrodes. A cover made of polydimethylsiloxane was designed with four wells whose open bottoms fitted over each set. Loading, culture and stimulation of cells were conducted within each well. After stimulation of macrophages by a calcium ionophore, amperometric responses were then monitored using a multipotentiostat to detect one species per well simultaneously. The results showed good reproducibility and were quite consistent with averaged amperometric detections obtained from single cell measurements. This methodology allowed drastically diminishing the number of experiments necessary to reach a statistical significance from n=100–160 experiments required for single cell detection compared to n=5 experiments to obtain the mean behavior of the cell population. This work demonstrated that this simple and versatile microchip could be used for quantification of species in oxidative stress investigations on cell populations as well as a complementary tool to compare behaviors between single cell and cell population for investigations involving many experimental variables. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim |
Real Time Monitoring of Peroxynitrite by Stimulation of Macrophages with Ultramicroelectrodes Book Chapter Christian Amatore; Manon Guille-Collignon; Frederic Lemaitre Peteu, S F; Szunerits, S; Bayachou, M (Ed.): Peroxynitrite Detection in Biological Media: Challenges and Advances, 7 , p. 96-120, 2016, (Times Cited: 0). @inbook{,
title = {Real Time Monitoring of Peroxynitrite by Stimulation of Macrophages with Ultramicroelectrodes},
author = {Christian Amatore and Manon Guille-Collignon and Frederic Lemaitre},
editor = {S F Peteu and S Szunerits and M Bayachou},
year = {2016},
date = {2016-01-01},
booktitle = {Peroxynitrite Detection in Biological Media: Challenges and Advances},
volume = {7},
pages = {96-120},
series = {RSC Detection Science Series},
note = {Times Cited: 0},
keywords = {},
pubstate = {published},
tppubtype = {inbook}
}
|
2015
|
Bioanalytical applications of the fluorescence-electrochemistry combination Article de journal F Lemaître; M Guille-Collignon Actualite Chimique, (400-401), p. 17–19, 2015. @article{Lemaitre:2015,
title = {Bioanalytical applications of the fluorescence-electrochemistry combination},
author = {F Lema\^{i}tre and M Guille-Collignon},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84973499998&partnerID=40&md5=d5fb99aaf2d3336a53a286546cb86bdb},
year = {2015},
date = {2015-01-01},
journal = {Actualite Chimique},
number = {400-401},
pages = {17--19},
abstract = {Both fluorescence and electrochemistry techniques aim at converting a chemical signal into an optical or an electrical one respectively. Particularly, they correspond to appropriate techniques for investigating biological phenomena due to the electroactivity of many biomolecules while cells or proteins can be labeled with fluorophores. Therefore, this article is a non exhaustive presentation of the coupling between electrochemistry and fluorescence for biological investigations. By focusing on exocytosis, it also raises the question of the implementation of such a combination.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Both fluorescence and electrochemistry techniques aim at converting a chemical signal into an optical or an electrical one respectively. Particularly, they correspond to appropriate techniques for investigating biological phenomena due to the electroactivity of many biomolecules while cells or proteins can be labeled with fluorophores. Therefore, this article is a non exhaustive presentation of the coupling between electrochemistry and fluorescence for biological investigations. By focusing on exocytosis, it also raises the question of the implementation of such a combination. |
Evaluation of photosynthetic electrons derivation by exogenous redox mediators Article de journal Guillaume Longatte; Han-Yi Fu; Olivier Buriez; Eric Labbé; Francis-André Wollman; Christian Amatore; Fabrice Rappaport; Manon Guille-Collignon; Frédéric Lemaître Biophysical Chemistry, 205 , p. 1-8, 2015. @article{RID:0721150706473-48b,
title = {Evaluation of photosynthetic electrons derivation by exogenous redox mediators},
author = {Guillaume Longatte and Han-Yi Fu and Olivier Buriez and Eric Labb\'{e} and Francis-Andr\'{e} Wollman and Christian Amatore and Fabrice Rappaport and Manon Guille-Collignon and Fr\'{e}d\'{e}ric Lema\^{i}tre},
year = {2015},
date = {2015-01-01},
journal = {Biophysical Chemistry},
volume = {205},
pages = {1-8},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Three-electrode analytical and preparative electrochemistry in micro-volume hanging droplets Article de journal Ana Isabel Perez Jimenez; Lylian Challier; Margherita Di Pisa; Manon Guille-Collignon; Frédéric Lemaître; Solange Lavielle; Christelle Mansuy; Christian Amatore; Eric Labbé; Olivier Buriez Electrochemistry Communications, 54 (0), p. 41-45, 2015. @article{RID:0721150706473-46b,
title = {Three-electrode analytical and preparative electrochemistry in micro-volume hanging droplets},
author = {Ana Isabel Perez Jimenez and Lylian Challier and Margherita Di Pisa and Manon Guille-Collignon and Fr\'{e}d\'{e}ric Lema\^{i}tre and Solange Lavielle and Christelle Mansuy and Christian Amatore and Eric Labb\'{e} and Olivier Buriez},
year = {2015},
date = {2015-01-01},
journal = {Electrochemistry Communications},
volume = {54},
number = {0},
pages = {41-45},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Vesicular Exocytosis and Microdevices - Microelectrode Arrays Article de journal Christian Amatore; Jerome Delacotte; Manon Guille-Collignon; Frederic Lemaitre Analyst, 140 (11), p. 3687-3695, 2015, ISSN: 0003-2654, (WOS:000354650300003). @article{Amatore:2015,
title = {Vesicular Exocytosis and Microdevices - Microelectrode Arrays},
author = {Christian Amatore and Jerome Delacotte and Manon {Guille-Collignon} and Frederic Lemaitre},
doi = {10.1039/c4an01932f},
issn = {0003-2654},
year = {2015},
date = {2015-01-01},
journal = {Analyst},
volume = {140},
number = {11},
pages = {3687-3695},
abstract = {Among all the analytical techniques capable of monitoring exocytosis in real time at the single cell level, electrochemistry (particularly amperometry at a constant potential) using ultramicroelectrodes has been demonstrated to be an important and convenient tool for more than two decades. Indeed, because the electrochemical sensor is located in the close vicinity of the emitting cell ("artificial synapse" configuration), much data can be gathered from the whole cell activity (secretion frequency) to the individual vesicular release (duration, fluxes or amount of molecules released) with an excellent sensitivity. However, such a single cell analysis and its intrinsic benefits are at the expense of the spatial resolution and/or the number of experiments. The quite recent development of microdevices/microsystems (and mainly the microelectrode arrays (MEAs)) offers in some way a complementary approach either by combining spectroscopy-microscopy or by implementing a multianalysis. Such developments are described and discussed in the present review over the 2005-2014 period.},
note = {WOS:000354650300003},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Among all the analytical techniques capable of monitoring exocytosis in real time at the single cell level, electrochemistry (particularly amperometry at a constant potential) using ultramicroelectrodes has been demonstrated to be an important and convenient tool for more than two decades. Indeed, because the electrochemical sensor is located in the close vicinity of the emitting cell ("artificial synapse" configuration), much data can be gathered from the whole cell activity (secretion frequency) to the individual vesicular release (duration, fluxes or amount of molecules released) with an excellent sensitivity. However, such a single cell analysis and its intrinsic benefits are at the expense of the spatial resolution and/or the number of experiments. The quite recent development of microdevices/microsystems (and mainly the microelectrode arrays (MEAs)) offers in some way a complementary approach either by combining spectroscopy-microscopy or by implementing a multianalysis. Such developments are described and discussed in the present review over the 2005-2014 period. |
2014
|
Amperometric Detection of Vesicular Exocytosis from BON Cells at Carbon Fiber Microelectrodes Article de journal Anne Meunier; Marine Bretou; Francois Darchen; Manon Guille Collignon; Frederic Lemaitre; Christian Amatore Electrochimica Acta, 126 , p. 74-80, 2014, ISSN: 0013-4686. @article{RN25b,
title = {Amperometric Detection of Vesicular Exocytosis from BON Cells at Carbon Fiber Microelectrodes},
author = {Anne Meunier and Marine Bretou and Francois Darchen and Manon Guille Collignon and Frederic Lemaitre and Christian Amatore},
doi = {10.1016/j.electacta.2013.07.110},
issn = {0013-4686},
year = {2014},
date = {2014-01-01},
journal = {Electrochimica Acta},
volume = {126},
pages = {74-80},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Anti-oxidant Mn-complexes: evaluation in cellular models of oxidative stress Article de journal Clotilde Policar; Anne-Sophie Bernard; Nicolas Delsuc; Geraldine Gazzah; Manon Guille; Frederic Lemaitre; Christian Amatore; Maria Bachelet; Joelle Masliah Journal of Biological Inorganic Chemistry, 19 , p. S739-S740, 2014, (Times Cited: 0
2
12th European Biological Inorganic Chemistry Conference (EuroBIC)
Aug 24-28, 2014
Zurich, SWITZERLAND
Univ Zurich). @article{,
title = {Anti-oxidant Mn-complexes: evaluation in cellular models of oxidative stress},
author = {Clotilde Policar and Anne-Sophie Bernard and Nicolas Delsuc and Geraldine Gazzah and Manon Guille and Frederic Lemaitre and Christian Amatore and Maria Bachelet and Joelle Masliah},
year = {2014},
date = {2014-01-01},
journal = {Journal of Biological Inorganic Chemistry},
volume = {19},
pages = {S739-S740},
note = {Times Cited: 0
2
12th European Biological Inorganic Chemistry Conference (EuroBIC)
Aug 24-28, 2014
Zurich, SWITZERLAND
Univ Zurich},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Electrochemical detection of nitric oxide and peroxynitrite anion in microchannels at highly sensitive platinum-black coated electrodes. application to ROS and RNS mixtures prior to biological investigations Article de journal Y Li; C Sella; F Lemaître; M Guille-Collignon; L Thouin; C Amatore Electrochimica Acta, 144 , p. 111–118, 2014. @article{Li:2014,
title = {Electrochemical detection of nitric oxide and peroxynitrite anion in microchannels at highly sensitive platinum-black coated electrodes. application to ROS and RNS mixtures prior to biological investigations},
author = {Y Li and C Sella and F Lema\^{i}tre and M Guille-Collignon and L Thouin and C Amatore},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84907101896&doi=10.1016%2fj.electacta.2014.08.046&partnerID=40&md5=2c011b35bc2ea90bd61dfef906786730},
doi = {10.1016/j.electacta.2014.08.046},
year = {2014},
date = {2014-01-01},
journal = {Electrochimica Acta},
volume = {144},
pages = {111--118},
abstract = {The electrochemical detection of nitric oxide (NO) and peroxynitrite anion (ONOO-) was investigated at Pt-black electrodes in microchannels. Owing to the high reactivity of these species under conditions close to physiological media, kinetic parameters were determined before evaluating the detection performances from synthetic solutions. Highly sensitive and stable Pt-black electrodes allowed detection limits down to 30 nM (NO) and 40 nM (ONOO-) to be reached with very high sensitivities. As NO and ONOO-are two relevant biological molecules involved in oxidative stress their simultaneous detections with other major molecules like hydrogen peroxide and nitrite were also performed and validated experimentally at pH 8.4. These results demonstrated that relative ROS/RNS contents in synthetic mixtures can be easily assessed at selected detection potentials. Beyond the interest of using small volumes in microfluidic channels, optimization of detection requires precise conditions easy to implement. These were delineated to lead in microdevices to high-performances detection of oxidative stress metabolites either from analytes or from the production of a few living cells like macrophages. © 2014 Elsevier Ltd.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The electrochemical detection of nitric oxide (NO) and peroxynitrite anion (ONOO-) was investigated at Pt-black electrodes in microchannels. Owing to the high reactivity of these species under conditions close to physiological media, kinetic parameters were determined before evaluating the detection performances from synthetic solutions. Highly sensitive and stable Pt-black electrodes allowed detection limits down to 30 nM (NO) and 40 nM (ONOO-) to be reached with very high sensitivities. As NO and ONOO-are two relevant biological molecules involved in oxidative stress their simultaneous detections with other major molecules like hydrogen peroxide and nitrite were also performed and validated experimentally at pH 8.4. These results demonstrated that relative ROS/RNS contents in synthetic mixtures can be easily assessed at selected detection potentials. Beyond the interest of using small volumes in microfluidic channels, optimization of detection requires precise conditions easy to implement. These were delineated to lead in microdevices to high-performances detection of oxidative stress metabolites either from analytes or from the production of a few living cells like macrophages. © 2014 Elsevier Ltd. |
