Professeur des Universités, Sorbonne Université
Responsable du pôle de Chimie Physique et Biologique de la Matière Vivante
PASTEUR, Département de chimie, École Normale Supérieure, PSL University, Sorbonne Université, CNRS
24 rue Lhomond, 75005 Paris, France
Email: Ludovic.Jullien@ens.psl.eu or Ludovic.Jullien@sorbonne-universite.fr
Phone: +33 144323333
Office: E142c
Home page: https://ludovicjullien.org/
Publications
2011 |
Temperature modulated excitation and phase sensitive detection to selectively image DNA sequences Inproceedings K Zrelli; E Cavatore; T Barilero; H Berthoumieux; V Croquette; T Le Saux; L Jullien; A Lemarchand; C Gosse 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11, p. 2160–2163, 2011. @inproceedings{Zrelli:2011b, title = {Temperature modulated excitation and phase sensitive detection to selectively image DNA sequences}, author = {K Zrelli and E Cavatore and T Barilero and H Berthoumieux and V Croquette and T Le Saux and L Jullien and A Lemarchand and C Gosse}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-80052114195&doi=10.1109%2fTRANSDUCERS.2011.5969322&partnerID=40&md5=405f3c87fbb8e4447dc5eb485cc046e8}, doi = {10.1109/TRANSDUCERS.2011.5969322}, year = {2011}, date = {2011-01-01}, booktitle = {2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11}, pages = {2160--2163}, abstract = {Temperature oscillations allow one to modulate the concentration of reagents and products with given amplitude and phase shift, both parameters depending on the dynamics of the chemical system under study. We here rely on thermal excitation in a microdevice, associated with fluorescence video microscopy as well as image filtering protocols, to selectively enhance the signal issued from a labeled probe that hybridizes with a targeted oligonucleotide. Furthermore, by simply tuning the stimulation parameters, focus can be put either on the perfect match sequence or on some other one that bears a point mutation. © 2011 IEEE.}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } Temperature oscillations allow one to modulate the concentration of reagents and products with given amplitude and phase shift, both parameters depending on the dynamics of the chemical system under study. We here rely on thermal excitation in a microdevice, associated with fluorescence video microscopy as well as image filtering protocols, to selectively enhance the signal issued from a labeled probe that hybridizes with a targeted oligonucleotide. Furthermore, by simply tuning the stimulation parameters, focus can be put either on the perfect match sequence or on some other one that bears a point mutation. © 2011 IEEE. |
Temperature modulation and quadrature detection for selective titration of two-state exchanging reactants Article de journal K Zrelli; T Barilero; E Cavatore; H Berthoumieux; T Le Saux; V Croquette; A Lemarchand; C Gosse; L Jullien Analytical Chemistry, 83 (7), p. 2476–2484, 2011. @article{Zrelli:2011a, title = {Temperature modulation and quadrature detection for selective titration of two-state exchanging reactants}, author = {K Zrelli and T Barilero and E Cavatore and H Berthoumieux and T Le Saux and V Croquette and A Lemarchand and C Gosse and L Jullien}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-79953280715&doi=10.1021%2fac1026034&partnerID=40&md5=27af1d31b99d901d7b3b782f16a0329c}, doi = {10.1021/ac1026034}, year = {2011}, date = {2011-01-01}, journal = {Analytical Chemistry}, volume = {83}, number = {7}, pages = {2476--2484}, abstract = {Biological samples exhibit huge molecular diversity over large concentration ranges. Titrating a given compound in such mixtures is often difficult, and innovative strategies emphasizing selectivity are thus demanded. To overcome limitations inherent to thermodynamics, we here present a generic technique where discrimination relies on the dynamics of interaction between the target of interest and a probe introduced in excess. Considering an ensemble of two-state exchanging reactants submitted to temperature modulation, we first demonstrate that the amplitude of the out-of-phase concentration oscillations is maximum for every compound involved in a reaction whose equilibrium constant is equal to unity and whose relaxation time is equal to the inverse of the excitation angular frequency. Taking advantage of this feature, we next devise a highly specific detection protocol and validate it using a microfabricated resistive heater and an epifluorescence microscope, as well as labeled oligonucleotides to model species displaying various dynamic properties. As expected, quantification of a sought for strand is obtained even if interfering reagents are present in similar amounts. Moreover, our approach does not require any separation and is compatible with imaging. It could then benefit some of the numerous binding assays performed every day in life sciences. © 2011 American Chemical Society.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Biological samples exhibit huge molecular diversity over large concentration ranges. Titrating a given compound in such mixtures is often difficult, and innovative strategies emphasizing selectivity are thus demanded. To overcome limitations inherent to thermodynamics, we here present a generic technique where discrimination relies on the dynamics of interaction between the target of interest and a probe introduced in excess. Considering an ensemble of two-state exchanging reactants submitted to temperature modulation, we first demonstrate that the amplitude of the out-of-phase concentration oscillations is maximum for every compound involved in a reaction whose equilibrium constant is equal to unity and whose relaxation time is equal to the inverse of the excitation angular frequency. Taking advantage of this feature, we next devise a highly specific detection protocol and validate it using a microfabricated resistive heater and an epifluorescence microscope, as well as labeled oligonucleotides to model species displaying various dynamic properties. As expected, quantification of a sought for strand is obtained even if interfering reagents are present in similar amounts. Moreover, our approach does not require any separation and is compatible with imaging. It could then benefit some of the numerous binding assays performed every day in life sciences. © 2011 American Chemical Society. |
2010 |
2-Hydroxyazobenzenes to tailor pH Pulses and oscillations with light Article de journal M Emond; T L Saux; S Maurin; J -B Baudin; R Plasson; L Jullien Chemistry - A European Journal, 16 (29), p. 8822–8831, 2010. @article{Emond:2010, title = {2-Hydroxyazobenzenes to tailor pH Pulses and oscillations with light}, author = {M Emond and T L Saux and S Maurin and J -B Baudin and R Plasson and L Jullien}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77955126007&doi=10.1002%2fchem.201000541&partnerID=40&md5=dcfd1d6c4e29b0265d1f1b84f612f306}, doi = {10.1002/chem.201000541}, year = {2010}, date = {2010-01-01}, journal = {Chemistry - A European Journal}, volume = {16}, number = {29}, pages = {8822--8831}, abstract = {This paper evaluates the 2hydroxyazobenzene platform for tailoring proton concentration pulses and oscillations with monochromatic light. The easily prepared 2-hydroxyazobenzenes exhibit large absorptions in the near-UV range. Photoisomerization was investigated by UV/Vis absorption, 1H NMR spectroscopy, and steady-state fluorescence emission. In the whole investigated series, the trans stereoisomer of the 2-hydroxyazobenzene motif provides the corresponding cis derivative with an action cross section in the 10 3M-1cm-1 range. At the same time, photoisomerization is accompanied by a significant pK drop of the phenol group. According to the phenyl-substituent pattern, cis-to-trans thermal back-isomerization can be tuned in the 10 ms-100 s range. Up to 2 units of reversible pH drops or pH oscillations on the 10 s timescale have been obtained by appropriately tailoring single-wavelength illumination of 2-hydroxyazobenzene solutions. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA. Weinheim.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This paper evaluates the 2hydroxyazobenzene platform for tailoring proton concentration pulses and oscillations with monochromatic light. The easily prepared 2-hydroxyazobenzenes exhibit large absorptions in the near-UV range. Photoisomerization was investigated by UV/Vis absorption, 1H NMR spectroscopy, and steady-state fluorescence emission. In the whole investigated series, the trans stereoisomer of the 2-hydroxyazobenzene motif provides the corresponding cis derivative with an action cross section in the 10 3M-1cm-1 range. At the same time, photoisomerization is accompanied by a significant pK drop of the phenol group. According to the phenyl-substituent pattern, cis-to-trans thermal back-isomerization can be tuned in the 10 ms-100 s range. Up to 2 units of reversible pH drops or pH oscillations on the 10 s timescale have been obtained by appropriately tailoring single-wavelength illumination of 2-hydroxyazobenzene solutions. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA. Weinheim. |
Photoactivation of the CreERT2 recombinase for conditional site-specific recombination with high spatiotemporal resolution Article de journal D K Sinha; P Neveu; N Gagey; I Aujard; T Le Saux; C Rampon; C Gauron; K Kawakami; C Leucht; L Bally-Cuif; M Volovitch; D Bensimon; L Jullien; S Vriz Zebrafish, 7 (2), p. 199–204, 2010. @article{Sinha:2010a, title = {Photoactivation of the CreERT2 recombinase for conditional site-specific recombination with high spatiotemporal resolution}, author = {D K Sinha and P Neveu and N Gagey and I Aujard and T Le Saux and C Rampon and C Gauron and K Kawakami and C Leucht and L Bally-Cuif and M Volovitch and D Bensimon and L Jullien and S Vriz}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77953623529&doi=10.1089%2fzeb.2009.0632&partnerID=40&md5=e5fb039a397abb6510140bcd6b2b258b}, doi = {10.1089/zeb.2009.0632}, year = {2010}, date = {2010-01-01}, journal = {Zebrafish}, volume = {7}, number = {2}, pages = {199--204}, abstract = {We implemented a noninvasive optical method for the fast control of Cre recombinase in single cells of a live zebrafish embryo. Optical uncaging of the caged precursor of a nonendogeneous steroid by one- or two-photon illumination was used to restore Cre activity of the CreERT2 fusion protein in specific target cells. This method labels single cells irreversibly by inducing recombination in an appropriate reporter transgenic animal and thereby can achieve high spatiotemporal resolution in the control of gene expression. This technique could be used more generally to investigate important physiological processes (e.g., in embryogenesis, organ regeneration, or carcinogenesis) with high spatiotemporal resolution (single cell and 10-min scales). © Copyright 2010, Mary Ann Liebert, Inc.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We implemented a noninvasive optical method for the fast control of Cre recombinase in single cells of a live zebrafish embryo. Optical uncaging of the caged precursor of a nonendogeneous steroid by one- or two-photon illumination was used to restore Cre activity of the CreERT2 fusion protein in specific target cells. This method labels single cells irreversibly by inducing recombination in an appropriate reporter transgenic animal and thereby can achieve high spatiotemporal resolution in the control of gene expression. This technique could be used more generally to investigate important physiological processes (e.g., in embryogenesis, organ regeneration, or carcinogenesis) with high spatiotemporal resolution (single cell and 10-min scales). © Copyright 2010, Mary Ann Liebert, Inc. |
Photocontrol of protein activity in cultured cells and zebrafish with one- and two-photon illumination Article de journal D K Sinha; P Neveu; N Gagey; I Aujard; C Benbrahim-Bouzidi; T Le Saux; C Rampon; C Gauron; B Goetz; S Dubruille; M Baaden; M Volovitch; D Bensimon; S Vriz; L Jullien ChemBioChem, 11 (5), p. 653–663, 2010. @article{Sinha:2010, title = {Photocontrol of protein activity in cultured cells and zebrafish with one- and two-photon illumination}, author = {D K Sinha and P Neveu and N Gagey and I Aujard and C Benbrahim-Bouzidi and T Le Saux and C Rampon and C Gauron and B Goetz and S Dubruille and M Baaden and M Volovitch and D Bensimon and S Vriz and L Jullien}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77949823382&doi=10.1002%2fcbic.201000008&partnerID=40&md5=4ac50ebf9ae4f10b80cc91f729b23969}, doi = {10.1002/cbic.201000008}, year = {2010}, date = {2010-01-01}, journal = {ChemBioChem}, volume = {11}, number = {5}, pages = {653--663}, abstract = {We have implemented a noninvasive optical method for the fast control of protein activity in a live zebrafish embryo. It relies on releasing a protein fused to a modified estrogen receptor ligand binding domain from its complex with cytoplasmic chaperones, upon the local photoactivation of a nonendogenous caged inducer. Molecular dynamics simulations were used to design cyclofen-OH, a photochemically stable inducer of the receptor specific for 4-hydroxy-tamoxifen (ERT2). Cyclofen-OH was easily synthesized in two steps with good yields. At submicromolar concentrations, it activates proteins fused to the ERT2 receptor. This was shown in cultured cells and in zebrafish embryos through emission properties and subcellular localization of properly engineered fluorescent proteins. Cyclofen-OH was successfully caged with various photolabile protecting groups. One particular caged compound was efficient in photoinducing the nuclear translocation of fluorescent proteins either globally (with 365 nm UV illumination) or locally (with a focused UV laser or with two-photon illumination at 750 nm). The present method for photocontrol of protein activity could be used more generally to investigate important physiological processes (e.g., in embryogenesis, organ regeneration and carcinogenesis) with high spatiotemporal resolution. © 2010 Wiley-VCH Verlag GmbH& Co. KGaA.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We have implemented a noninvasive optical method for the fast control of protein activity in a live zebrafish embryo. It relies on releasing a protein fused to a modified estrogen receptor ligand binding domain from its complex with cytoplasmic chaperones, upon the local photoactivation of a nonendogenous caged inducer. Molecular dynamics simulations were used to design cyclofen-OH, a photochemically stable inducer of the receptor specific for 4-hydroxy-tamoxifen (ERT2). Cyclofen-OH was easily synthesized in two steps with good yields. At submicromolar concentrations, it activates proteins fused to the ERT2 receptor. This was shown in cultured cells and in zebrafish embryos through emission properties and subcellular localization of properly engineered fluorescent proteins. Cyclofen-OH was successfully caged with various photolabile protecting groups. One particular caged compound was efficient in photoinducing the nuclear translocation of fluorescent proteins either globally (with 365 nm UV illumination) or locally (with a focused UV laser or with two-photon illumination at 750 nm). The present method for photocontrol of protein activity could be used more generally to investigate important physiological processes (e.g., in embryogenesis, organ regeneration and carcinogenesis) with high spatiotemporal resolution. © 2010 Wiley-VCH Verlag GmbH& Co. KGaA. |
Temperature modulation and phase sensitive imaging to detect point mutations Inproceedings K Zrelli; T Barilero; E Cavatore; H Berthoumieux; V Croquette; A Lemarchand; L Jullien; T Le Saux; C Gosse 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, p. 1271–1273, 2010. @inproceedings{Zrelli:2010, title = {Temperature modulation and phase sensitive imaging to detect point mutations}, author = {K Zrelli and T Barilero and E Cavatore and H Berthoumieux and V Croquette and A Lemarchand and L Jullien and T Le Saux and C Gosse}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84884400328&partnerID=40&md5=25428d835811e78ea89388612cd16b98}, year = {2010}, date = {2010-01-01}, booktitle = {14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010}, volume = {2}, pages = {1271--1273}, abstract = {Temperature oscillations allow one to modulate the concentration of reagents and products with given amplitude and phase shift, both parameters depending on the dynamics of the chemical system under study. We here rely on thermal excitation in a microdevice, associated with fluorescence video microscopy as well as image filtering protocols, to selectively enhance the signal issued from a labeled probe that hybridizes with a targeted oligonucleotide. Furthermore, by simply tuning the stimulation parameters, focus can be put either on the perfect match sequence or on some other one that bears a point mutation.}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } Temperature oscillations allow one to modulate the concentration of reagents and products with given amplitude and phase shift, both parameters depending on the dynamics of the chemical system under study. We here rely on thermal excitation in a microdevice, associated with fluorescence video microscopy as well as image filtering protocols, to selectively enhance the signal issued from a labeled probe that hybridizes with a targeted oligonucleotide. Furthermore, by simply tuning the stimulation parameters, focus can be put either on the perfect match sequence or on some other one that bears a point mutation. |
2009 |
A two-color molecular beacon for microchip thermal mapping Inproceedings T Le Saux; T Barilero; O Jouannot; L Jullien; C Gosse Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, p. 2025–2027, 2009. @inproceedings{LeSaux:2009, title = {A two-color molecular beacon for microchip thermal mapping}, author = {T Le Saux and T Barilero and O Jouannot and L Jullien and C Gosse}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84901775127&partnerID=40&md5=60be07b422860df20d0c23c9a147212d}, year = {2009}, date = {2009-01-01}, booktitle = {Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences}, pages = {2025--2027}, abstract = {We introduce a molecular thermometer for ratiometric dual-emission- wavelength measurements. Thermal mapping is performed by epifluorescence microscopy, the acquisition and data treatment procedures ensuring results that neither depend on the probe concentration or on the observation setup imperfections. As an application, we demonstrate Joule heating monitoring during electrokinetic injection. © 2009 CBMS.}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } We introduce a molecular thermometer for ratiometric dual-emission- wavelength measurements. Thermal mapping is performed by epifluorescence microscopy, the acquisition and data treatment procedures ensuring results that neither depend on the probe concentration or on the observation setup imperfections. As an application, we demonstrate Joule heating monitoring during electrokinetic injection. © 2009 CBMS. |
Fluorescent thermometers for dual-emission-wavelength measurements: Molecular engineering and application to thermal imaging in a microsystem Article de journal T Barilero; T Le Saux; C Gosse; L Jullien Analytical Chemistry, 81 (19), p. 7988–8000, 2009. @article{Barilero:2009, title = {Fluorescent thermometers for dual-emission-wavelength measurements: Molecular engineering and application to thermal imaging in a microsystem}, author = {T Barilero and T Le Saux and C Gosse and L Jullien}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-70349624352&doi=10.1021%2fac901027f&partnerID=40&md5=79e5948e7f33f1573c5bd1570160215c}, doi = {10.1021/ac901027f}, year = {2009}, date = {2009-01-01}, journal = {Analytical Chemistry}, volume = {81}, number = {19}, pages = {7988--8000}, abstract = {To facilitate thermal imaging, particularly in microdevices, one has to favor molecular thermometers in which the response is independent of the probe concentration and of the observation setup imperfections. Hence, this paper introduces two temperature fluorescent probes for ratiometric dual-emission-wavelength measurements in aqueous solutions. They are based on a nonathermal chemical reaction, either a conformational transition or a protonation, that induces a modification of their emission spectra as the temperature changes. Relying on both a straightforward theoretical analysis and thorough photophysical, thermodynamic, and kinetic investigations, we demonstrate how the flexible design of these two thermometers can be optimized to face applications with various requirements in terms of operating temperature and wavelength ranges as well as temporal resolution. For instance, the present molecules, which can be used between 5 and 35°C, provide a relative sensitivity up to ∼ 9 × 10-2 K-1 and milli- to microsecond response times. Finally, we utilize a two-color molecular beacon, a probe belonging to the first series of thermometers, to image temperature profiles in a microfluidic cell heated by a resistive strip. The ratiometric analysis of the fluorescence emission at two different wavelengths is performed on a widely available dual-view microscope, illustrating both the simplicity and reliability of the thermal mapping protocol. © 2009 American Chemical Society.}, keywords = {}, pubstate = {published}, tppubtype = {article} } To facilitate thermal imaging, particularly in microdevices, one has to favor molecular thermometers in which the response is independent of the probe concentration and of the observation setup imperfections. Hence, this paper introduces two temperature fluorescent probes for ratiometric dual-emission-wavelength measurements in aqueous solutions. They are based on a nonathermal chemical reaction, either a conformational transition or a protonation, that induces a modification of their emission spectra as the temperature changes. Relying on both a straightforward theoretical analysis and thorough photophysical, thermodynamic, and kinetic investigations, we demonstrate how the flexible design of these two thermometers can be optimized to face applications with various requirements in terms of operating temperature and wavelength ranges as well as temporal resolution. For instance, the present molecules, which can be used between 5 and 35°C, provide a relative sensitivity up to ∼ 9 × 10-2 K-1 and milli- to microsecond response times. Finally, we utilize a two-color molecular beacon, a probe belonging to the first series of thermometers, to image temperature profiles in a microfluidic cell heated by a resistive strip. The ratiometric analysis of the fluorescence emission at two different wavelengths is performed on a widely available dual-view microscope, illustrating both the simplicity and reliability of the thermal mapping protocol. © 2009 American Chemical Society. |
Resonant response to temperature modulation for enzymatic dynamics characterization Article de journal H Berthoumieux; C Antoine; L Jullien; A Lemarchand Physical Review E - Statistical, Nonlinear, and Soft Matter Physics, 79 (2), 2009. @article{Berthoumieux:2009, title = {Resonant response to temperature modulation for enzymatic dynamics characterization}, author = {H Berthoumieux and C Antoine and L Jullien and A Lemarchand}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-61549099297&doi=10.1103%2fPhysRevE.79.021906&partnerID=40&md5=bde7b045fc3c9d9f1d85e3ffe3d07a17}, doi = {10.1103/PhysRevE.79.021906}, year = {2009}, date = {2009-01-01}, journal = {Physical Review E - Statistical, Nonlinear, and Soft Matter Physics}, volume = {79}, number = {2}, abstract = {We consider enzymes involved in a three-state Michaelis-Menten kinetics and submitted to well-chosen temperature modulations of small amplitude. From the first-order amplitudes of concentration oscillations, we design a response function that is maximum for targeted values of the chemical relaxation times. This resonant function can be used to screen a large set of enzymes and identify the one governed by the desired kinetics. The method gives access to all the dynamical parameters of the targeted enzyme without resorting to a fit. We show how to estimate the precision of this parameter determination and give some hints for experimental validation. © 2009 The American Physical Society.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We consider enzymes involved in a three-state Michaelis-Menten kinetics and submitted to well-chosen temperature modulations of small amplitude. From the first-order amplitudes of concentration oscillations, we design a response function that is maximum for targeted values of the chemical relaxation times. This resonant function can be used to screen a large set of enzymes and identify the one governed by the desired kinetics. The method gives access to all the dynamical parameters of the targeted enzyme without resorting to a fit. We show how to estimate the precision of this parameter determination and give some hints for experimental validation. © 2009 The American Physical Society. |
Thermal modulation and filtered video acquisition to image reactive species among non reactive ones Inproceedings K Zrelli; T Barilero; T Le Saux; L Jullien; H Berthoumieux; A Lemarchand; C Gosse Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, p. 958–960, 2009. @inproceedings{Zrelli:2009, title = {Thermal modulation and filtered video acquisition to image reactive species among non reactive ones}, author = {K Zrelli and T Barilero and T Le Saux and L Jullien and H Berthoumieux and A Lemarchand and C Gosse}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84901796758&partnerID=40&md5=4d1969e49d590169bf74d7e23b252ed9}, year = {2009}, date = {2009-01-01}, booktitle = {Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences}, pages = {958--960}, abstract = {Periodic temperature excitation allows for modulating the concentration of a reagent with given amplitude and phase shift, both related of its characteristic chemical dynamics [1]. Therefore, we rely on thermal oscillations, associated with appropriate detection in fluorescence microscopy and data filtering, to selectively image a duplex forming oligonucleotide in a sample that contains non-hybridizing sequences in similar amount. © 2009 CBMS.}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } Periodic temperature excitation allows for modulating the concentration of a reagent with given amplitude and phase shift, both related of its characteristic chemical dynamics [1]. Therefore, we rely on thermal oscillations, associated with appropriate detection in fluorescence microscopy and data filtering, to selectively image a duplex forming oligonucleotide in a sample that contains non-hybridizing sequences in similar amount. © 2009 CBMS. |
2008 |
A caged retinoic acid for one- and two-photon excitation in zebrafish embryos Article de journal P Neveu; I Aujard; C Benbrahim; T Le Saux; J -F Allemand; S Vriz; D Bensimon; L Jullien Angewandte Chemie - International Edition, 47 (20), p. 3744–3746, 2008. @article{Neveu:2008, title = {A caged retinoic acid for one- and two-photon excitation in zebrafish embryos}, author = {P Neveu and I Aujard and C Benbrahim and T Le Saux and J -F Allemand and S Vriz and D Bensimon and L Jullien}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-45549089482&doi=10.1002%2fanie.200800037&partnerID=40&md5=320b837fbef96180278af6d16a13797a}, doi = {10.1002/anie.200800037}, year = {2008}, date = {2008-01-01}, journal = {Angewandte Chemie - International Edition}, volume = {47}, number = {20}, pages = {3744--3746}, abstract = {(Chemical Equation Presented) Escaping the cage: Retinoic acid (RA), a crucial signaling molecule for the embryogenesis of vertebrates, can be photoreleased from a simple caged derivative (cRA) upon illumination. In zebrafish embryos, cRA causes RA-induced phenotypes with one- and two-photon excitation (see picture), which opens a route to the noninvasive generation of controlled RA concentration patterns in vivo. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.}, keywords = {}, pubstate = {published}, tppubtype = {article} } (Chemical Equation Presented) Escaping the cage: Retinoic acid (RA), a crucial signaling molecule for the embryogenesis of vertebrates, can be photoreleased from a simple caged derivative (cRA) upon illumination. In zebrafish embryos, cRA causes RA-induced phenotypes with one- and two-photon excitation (see picture), which opens a route to the noninvasive generation of controlled RA concentration patterns in vivo. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA. |
Alcohol uncaging with fluorescence reporting: Evaluation of o-acetoxyphenyl methyloxazolone precursors Article de journal N Gagey; M Emond; P Neveu; C Benbrahim; B Goetz; I Aujard; J -B Baudin; L Jullien Organic Letters, 10 (12), p. 2341–2344, 2008. @article{Gagey:2008, title = {Alcohol uncaging with fluorescence reporting: Evaluation of o-acetoxyphenyl methyloxazolone precursors}, author = {N Gagey and M Emond and P Neveu and C Benbrahim and B Goetz and I Aujard and J -B Baudin and L Jullien}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-48849106962&doi=10.1021%2fol800284g&partnerID=40&md5=ea3b7c88c69385542890143aea72f28a}, doi = {10.1021/ol800284g}, year = {2008}, date = {2008-01-01}, journal = {Organic Letters}, volume = {10}, number = {12}, pages = {2341--2344}, abstract = {(Chemical Equation Presented) This paper evaluates a series of photolabile protecting groups with built-in fluorescence reporting. They rely on readily available o-acetoxyphenyl methyloxazolones as activated precursors. Alcohol substrates are easily caged. The resulting photoactivable esters exhibit large one- and two-photon uncaging cross sections. The alcohol substrates are quantitatively released in a 1:1 molar ratio with a strongly fluorescent coumarin coproduct that serves as a reporter to quantify substrate delivery. © 2008 American Chemical Society.}, keywords = {}, pubstate = {published}, tppubtype = {article} } (Chemical Equation Presented) This paper evaluates a series of photolabile protecting groups with built-in fluorescence reporting. They rely on readily available o-acetoxyphenyl methyloxazolones as activated precursors. Alcohol substrates are easily caged. The resulting photoactivable esters exhibit large one- and two-photon uncaging cross sections. The alcohol substrates are quantitatively released in a 1:1 molar ratio with a strongly fluorescent coumarin coproduct that serves as a reporter to quantify substrate delivery. © 2008 American Chemical Society. |
Fourier transform to analyse reaction-diffusion dynamics in a microsystem Article de journal A Estévez-Torres; T Le Saux; C Gosse; A Lemarchand; A Bourdoncle; L Jullien Lab on a Chip, 8 (7), p. 1205–1209, 2008. @article{Estevez-Torres:2008, title = {Fourier transform to analyse reaction-diffusion dynamics in a microsystem}, author = {A Est\'{e}vez-Torres and T Le Saux and C Gosse and A Lemarchand and A Bourdoncle and L Jullien}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-46149125030&doi=10.1039%2fb805412f&partnerID=40&md5=61edf13ed55e8879919bd89b45ba779a}, doi = {10.1039/b805412f}, year = {2008}, date = {2008-01-01}, journal = {Lab on a Chip}, volume = {8}, number = {7}, pages = {1205--1209}, abstract = {An integrated approach relying on a microsystem is introduced to easily extract, from a single experiment and with a global robust bi-exponential fit, an extensive set of thermodynamic, kinetic, and diffusion parameters governing associations in solution. © The Royal Society of Chemistry.}, keywords = {}, pubstate = {published}, tppubtype = {article} } An integrated approach relying on a microsystem is introduced to easily extract, from a single experiment and with a global robust bi-exponential fit, an extensive set of thermodynamic, kinetic, and diffusion parameters governing associations in solution. © The Royal Society of Chemistry. |
2007 |
Changes in the Morphology of Giant Vesicles Under Various Physico-Chemical Stresses incollection M -A Guedeau-Boudeville; A -L Bernard; J Bradley; A Singh; L Jullien Giant Vesicles: Perspectives in Supramolecular Chemistry, 6 , p. 341–349, 2007. @incollection{Guedeau-Boudeville:2007, title = {Changes in the Morphology of Giant Vesicles Under Various Physico-Chemical Stresses}, author = {M -A Guedeau-Boudeville and A -L Bernard and J Bradley and A Singh and L Jullien}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84955368046&doi=10.1002%2f9780470511534.ch26&partnerID=40&md5=630542a3e134f9fe44a60c1a944d2643}, doi = {10.1002/9780470511534.ch26}, year = {2007}, date = {2007-01-01}, booktitle = {Giant Vesicles: Perspectives in Supramolecular Chemistry}, volume = {6}, pages = {341--349}, keywords = {}, pubstate = {published}, tppubtype = {incollection} } |
Fourier analysis to measure diffusion coefficients and resolve mixtures on a continuous electrophoresis chip Article de journal A Estévez-Torres; C Gosse; T Le Saux; J -F Allemand; V Croquette; H Berthoumieux; A Lemarchand; L Jullien Analytical Chemistry, 79 (21), p. 8222–8231, 2007. @article{Estevez-Torres:2007, title = {Fourier analysis to measure diffusion coefficients and resolve mixtures on a continuous electrophoresis chip}, author = {A Est\'{e}vez-Torres and C Gosse and T Le Saux and J -F Allemand and V Croquette and H Berthoumieux and A Lemarchand and L Jullien}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-35848961243&doi=10.1021%2fac070532z&partnerID=40&md5=a7d83124bd11f49b053fcdce6e8e4fcf}, doi = {10.1021/ac070532z}, year = {2007}, date = {2007-01-01}, journal = {Analytical Chemistry}, volume = {79}, number = {21}, pages = {8222--8231}, abstract = {We report a method to measure diffusion coefficients of fluorescent solutes in the 102-106 Da molecular mass range in a glass-PDMS chip. Upon applying a permanent electric field, the solute is introduced through a narrow channel into a wide analysis chamber where it migrates along the injection axis and diffuses in two dimensions. The diffusion coefficient is extracted after 1D Fourier transform of the resulting stationary concentration pattern. Analysis is straightforward, requiring no numerical integration or velocity field simulation. The diffusion coefficients measured for fluorescein, rhodamine green-labeled oligonucleotides, and YOYO-1-stained dsDNA fragments agree with the literature values and with our own fluorescence correlation spectroscopy measurements. As shown for 151 and 1257 base pair dsDNA mixtures, the present method allows us to rely on diffusion to quantitatively characterize the nature and the composition of binary mixtures. In particular, we implement a DNA hybridization assay to illustrate the efficiency of the proposed protocol for library screening. © 2007 American Chemical Society.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We report a method to measure diffusion coefficients of fluorescent solutes in the 102-106 Da molecular mass range in a glass-PDMS chip. Upon applying a permanent electric field, the solute is introduced through a narrow channel into a wide analysis chamber where it migrates along the injection axis and diffuses in two dimensions. The diffusion coefficient is extracted after 1D Fourier transform of the resulting stationary concentration pattern. Analysis is straightforward, requiring no numerical integration or velocity field simulation. The diffusion coefficients measured for fluorescein, rhodamine green-labeled oligonucleotides, and YOYO-1-stained dsDNA fragments agree with the literature values and with our own fluorescence correlation spectroscopy measurements. As shown for 151 and 1257 base pair dsDNA mixtures, the present method allows us to rely on diffusion to quantitatively characterize the nature and the composition of binary mixtures. In particular, we implement a DNA hybridization assay to illustrate the efficiency of the proposed protocol for library screening. © 2007 American Chemical Society. |