Professeur des Universités, Sorbonne Université
Responsable du pôle de Chimie Physique et Biologique de la Matière Vivante
PASTEUR, Département de chimie, École Normale Supérieure, PSL University, Sorbonne Université, CNRS
24 rue Lhomond, 75005 Paris, France
Email: Ludovic.Jullien@ens.psl.eu or Ludovic.Jullien@sorbonne-universite.fr
Phone: +33 144323333
Office: E142c
Home page: https://ludovicjullien.org/
Publications
2017 |
Hybrid fluorescent probes for imaging cellular proteins on demand Article de journal L Jullien; A Gautier Medecine/Sciences, 33 (6-7), p. 576–578, 2017. @article{Jullien:2017, title = {Hybrid fluorescent probes for imaging cellular proteins on demand}, author = {L Jullien and A Gautier}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85044173814&doi=10.1051%2fmedsci%2f20173306006&partnerID=40&md5=438f0ceb949779e8a90a779a37a1d890}, doi = {10.1051/medsci/20173306006}, year = {2017}, date = {2017-01-01}, journal = {Medecine/Sciences}, volume = {33}, number = {6-7}, pages = {576--578}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Optical control of tumor induction in the Zebrafish Article de journal Z Feng; S Nam; F Hamouri; I Aujard; B Ducos; S Vriz; M Volovitch; L Jullien; S Lin; S Weiss; D Bensimon Scientific Reports, 7 (1), 2017. @article{Feng:2017, title = {Optical control of tumor induction in the Zebrafish}, author = {Z Feng and S Nam and F Hamouri and I Aujard and B Ducos and S Vriz and M Volovitch and L Jullien and S Lin and S Weiss and D Bensimon}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028028076&doi=10.1038%2fs41598-017-09697-x&partnerID=40&md5=0590b4f2bc458a31e987356701b6c9f7}, doi = {10.1038/s41598-017-09697-x}, year = {2017}, date = {2017-01-01}, journal = {Scientific Reports}, volume = {7}, number = {1}, abstract = {The zebrafish has become an increasingly popular and valuable cancer model over the past few decades. While most zebrafish cancer models are generated by expressing mammalian oncogenes under tissue-specific promoters, here we describe a method that allows for the precise optical control of oncogene expression in live zebrafish. We utilize this technique to transiently or constitutively activate a typical human oncogene, kRASG12V, in zebrafish embryos and investigate the developmental and tumorigenic phenotypes. We demonstrate the spatiotemporal control of oncogene expression in live zebrafish, and characterize the different tumorigenic probabilities when kRASG12V is expressed transiently or constitutively at different developmental stages. Moreover, we show that light can be used to activate oncogene expression in selected tissues and single cells without tissue-specific promoters. Our work presents a novel approach to initiate and study cancer in zebrafish, and the high spatiotemporal resolution of this method makes it a valuable tool for studying cancer initiation from single cells. © 2017 The Author(s).}, keywords = {}, pubstate = {published}, tppubtype = {article} } The zebrafish has become an increasingly popular and valuable cancer model over the past few decades. While most zebrafish cancer models are generated by expressing mammalian oncogenes under tissue-specific promoters, here we describe a method that allows for the precise optical control of oncogene expression in live zebrafish. We utilize this technique to transiently or constitutively activate a typical human oncogene, kRASG12V, in zebrafish embryos and investigate the developmental and tumorigenic phenotypes. We demonstrate the spatiotemporal control of oncogene expression in live zebrafish, and characterize the different tumorigenic probabilities when kRASG12V is expressed transiently or constitutively at different developmental stages. Moreover, we show that light can be used to activate oncogene expression in selected tissues and single cells without tissue-specific promoters. Our work presents a novel approach to initiate and study cancer in zebrafish, and the high spatiotemporal resolution of this method makes it a valuable tool for studying cancer initiation from single cells. © 2017 The Author(s). |
Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations Article de journal J Quérard; R Zhang; Z Kelemen; M -A Plamont; X Xie; R Chouket; I Roemgens; Y Korepina; S Albright; E Ipendey; M Volovitch; H L Sladitschek; P Neveu; L Gissot; A Gautier; J -D Faure; V Croquette; T Le Saux; L Jullien Nature Communications, 8 (1), 2017. @article{Querard:2017a, title = {Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations}, author = {J Qu\'{e}rard and R Zhang and Z Kelemen and M -A Plamont and X Xie and R Chouket and I Roemgens and Y Korepina and S Albright and E Ipendey and M Volovitch and H L Sladitschek and P Neveu and L Gissot and A Gautier and J -D Faure and V Croquette and T Le Saux and L Jullien}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85031811446&doi=10.1038%2fs41467-017-00847-3&partnerID=40&md5=c320b3585a15085c192d20ee6faa36fa}, doi = {10.1038/s41467-017-00847-3}, year = {2017}, date = {2017-01-01}, journal = {Nature Communications}, volume = {8}, number = {1}, abstract = {We present speed out-of-phase imaging after optical modulation (OPIOM), which exploits reversible photoswitchable fluorophores as fluorescent labels and combines optimized periodic illumination with phase-sensitive detection to specifically retrieve the label signal. Speed OPIOM can extract the fluorescence emission from a targeted label in the presence of spectrally interfering fluorophores and autofluorescence. Up to four fluorescent proteins exhibiting a similar green fluorescence have been distinguished in cells either sequentially or in parallel. Speed OPIOM is compatible with imaging biological processes in real time in live cells. Finally speed OPIOM is not limited to microscopy but is relevant for remote imaging as well, in particular, under ambient light. Thus, speed OPIOM has proved to enable fast and quantitative live microscopic and remote-multiplexed fluorescence imaging of biological samples while filtering out noise, interfering fluorophores, as well as ambient light. © 2017 The Author(s).}, keywords = {}, pubstate = {published}, tppubtype = {article} } We present speed out-of-phase imaging after optical modulation (OPIOM), which exploits reversible photoswitchable fluorophores as fluorescent labels and combines optimized periodic illumination with phase-sensitive detection to specifically retrieve the label signal. Speed OPIOM can extract the fluorescence emission from a targeted label in the presence of spectrally interfering fluorophores and autofluorescence. Up to four fluorescent proteins exhibiting a similar green fluorescence have been distinguished in cells either sequentially or in parallel. Speed OPIOM is compatible with imaging biological processes in real time in live cells. Finally speed OPIOM is not limited to microscopy but is relevant for remote imaging as well, in particular, under ambient light. Thus, speed OPIOM has proved to enable fast and quantitative live microscopic and remote-multiplexed fluorescence imaging of biological samples while filtering out noise, interfering fluorophores, as well as ambient light. © 2017 The Author(s). |
Syntheses and kinetic studies of cyclisation-based self-immolative spacers Article de journal S Huvelle; A Alouane; T Le Saux; L Jullien; F Schmidt Organic and Biomolecular Chemistry, 15 (16), p. 3435–3443, 2017. @article{Huvelle:2017, title = {Syntheses and kinetic studies of cyclisation-based self-immolative spacers}, author = {S Huvelle and A Alouane and T Le Saux and L Jullien and F Schmidt}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85017654758&doi=10.1039%2fc7ob00121e&partnerID=40&md5=b3efbccb7d9af044089b4be18ab3b100}, doi = {10.1039/c7ob00121e}, year = {2017}, date = {2017-01-01}, journal = {Organic and Biomolecular Chemistry}, volume = {15}, number = {16}, pages = {3435--3443}, abstract = {Kinetic analysis of the disassembly of self-immolative spacers based on cyclisation processes was performed. Five compounds were synthesized belonging to two different series, and their kinetic constants were determined. Electron-donating substituents gave a slight acceleration but the main effect was steric, and the Thorpe-Ingold effect was indeed particularly effective. Comparison with the self-immolative spacers based on elimination processes showed that cyclisations gave comparable or lower rate, but the corresponding spacers are more difficult to modulate. © The Royal Society of Chemistry.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Kinetic analysis of the disassembly of self-immolative spacers based on cyclisation processes was performed. Five compounds were synthesized belonging to two different series, and their kinetic constants were determined. Electron-donating substituents gave a slight acceleration but the main effect was steric, and the Thorpe-Ingold effect was indeed particularly effective. Comparison with the self-immolative spacers based on elimination processes showed that cyclisations gave comparable or lower rate, but the corresponding spacers are more difficult to modulate. © The Royal Society of Chemistry. |
2016 |
Design and characterization of red fluorogenic push-pull chromophores holding great potential for bioimaging and biosensing Article de journal C Li; M -A Plamont; I Aujard; T Le Saux; L Jullien; A Gautier Organic and Biomolecular Chemistry, 14 (39), p. 9253–9261, 2016. @article{Li:2016, title = {Design and characterization of red fluorogenic push-pull chromophores holding great potential for bioimaging and biosensing}, author = {C Li and M -A Plamont and I Aujard and T Le Saux and L Jullien and A Gautier}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84990182453&doi=10.1039%2fc6ob01612j&partnerID=40&md5=7cea2c6d4aeeeef91e6a3a5bd0d6b5ad}, doi = {10.1039/c6ob01612j}, year = {2016}, date = {2016-01-01}, journal = {Organic and Biomolecular Chemistry}, volume = {14}, number = {39}, pages = {9253--9261}, abstract = {Fluorogenic chromophores have been used recently for fluorescence reporting and biosensing. Their ability to turn on upon specific interaction with a given target has been exploited in particular for the design of fluorogen-based reporters enabling biomolecule labeling and imaging. In this paper, we report the development and exhaustive characterization of a new family of red fluorogenic push-pull chromophores, holding great potential for the development of fluorogen-based reporters or intracellular fluorogenic markers. The proposed methodology is generic and should find general applicability in the discovery of new fluorogenic dyes suitable for the design of fluorogen-based reporters and biosensors. © The Royal Society of Chemistry 2016.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Fluorogenic chromophores have been used recently for fluorescence reporting and biosensing. Their ability to turn on upon specific interaction with a given target has been exploited in particular for the design of fluorogen-based reporters enabling biomolecule labeling and imaging. In this paper, we report the development and exhaustive characterization of a new family of red fluorogenic push-pull chromophores, holding great potential for the development of fluorogen-based reporters or intracellular fluorogenic markers. The proposed methodology is generic and should find general applicability in the discovery of new fluorogenic dyes suitable for the design of fluorogen-based reporters and biosensors. © The Royal Society of Chemistry 2016. |
Kinetics of Reactive Modules Adds Discriminative Dimensions for Selective Cell Imaging Article de journal J Quérard; T Le Saux; A Gautier; D Alcor; V Croquette; A Lemarchand; C Gosse; L Jullien ChemPhysChem, 17 (10), p. 1396–1413, 2016. @article{Querard:2016, title = {Kinetics of Reactive Modules Adds Discriminative Dimensions for Selective Cell Imaging}, author = {J Qu\'{e}rard and T Le Saux and A Gautier and D Alcor and V Croquette and A Lemarchand and C Gosse and L Jullien}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84957539317&doi=10.1002%2fcphc.201500987&partnerID=40&md5=4a514250af842ae7697b695eb73769fb}, doi = {10.1002/cphc.201500987}, year = {2016}, date = {2016-01-01}, journal = {ChemPhysChem}, volume = {17}, number = {10}, pages = {1396--1413}, abstract = {Living cells are chemical mixtures of exceptional interest and significance, whose investigation requires the development of powerful analytical tools fulfilling the demanding constraints resulting from their singular features. In particular, multiplexed observation of a large number of molecular targets with high spatiotemporal resolution appears highly desirable. One attractive road to address this analytical challenge relies on engaging the targets in reactions and exploiting the rich kinetic signature of the resulting reactive module, which originates from its topology and its rate constants. This review explores the various facets of this promising strategy. We first emphasize the singularity of the content of a living cell as a chemical mixture and suggest that its multiplexed observation is significant and timely. Then, we show that exploiting the kinetics of analytical processes is relevant to selectively detect a given analyte: upon perturbing the system, the kinetic window associated to response read-out has to be matched with that of the targeted reactive module. Eventually, we introduce the state-of-the-art of cell imaging exploiting protocols based on reaction kinetics and draw some promising perspectives. Live recording: In living cells, the multiplexed observation of a large number of targets has recently emerged as a desirable goal. To address this challenge, an attractive road relies on engaging the targets in reactions and exploiting the kinetic signature of the resulting reactive module. This review explores the facets of this strategy and draw some promising perspectives. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Living cells are chemical mixtures of exceptional interest and significance, whose investigation requires the development of powerful analytical tools fulfilling the demanding constraints resulting from their singular features. In particular, multiplexed observation of a large number of molecular targets with high spatiotemporal resolution appears highly desirable. One attractive road to address this analytical challenge relies on engaging the targets in reactions and exploiting the rich kinetic signature of the resulting reactive module, which originates from its topology and its rate constants. This review explores the various facets of this promising strategy. We first emphasize the singularity of the content of a living cell as a chemical mixture and suggest that its multiplexed observation is significant and timely. Then, we show that exploiting the kinetics of analytical processes is relevant to selectively detect a given analyte: upon perturbing the system, the kinetic window associated to response read-out has to be matched with that of the targeted reactive module. Eventually, we introduce the state-of-the-art of cell imaging exploiting protocols based on reaction kinetics and draw some promising perspectives. Live recording: In living cells, the multiplexed observation of a large number of targets has recently emerged as a desirable goal. To address this challenge, an attractive road relies on engaging the targets in reactions and exploiting the kinetic signature of the resulting reactive module. This review explores the facets of this strategy and draw some promising perspectives. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Small Fluorescence-Activating and Absorption-Shifting Tag for Tunable Protein Imaging in Vivo Article de journal Marie-Aude Plamont; Emmanuelle Billon-Denis; Sylvie Maurin; Carole Gauron; Frederico M Pimenta; Christian G Specht; Jian Shi; Jérôme Querard; Buyan Pan; Julien Rossignol; Karine Moncoq; Nelly Morellet; Michel Volovitch; Ewen Lescop; Yong Chen; Antoine Triller; Sophie Vriz; Thomas Le Saux; Ludovic Jullien; Arnaud Gautier Proceedings of the National Academy of Sciences, 113 (3), p. 497, 2016. @article{RN50, title = {Small Fluorescence-Activating and Absorption-Shifting Tag for Tunable Protein Imaging in Vivo}, author = {Marie-Aude Plamont and Emmanuelle {Billon-Denis} and Sylvie Maurin and Carole Gauron and Frederico M Pimenta and Christian G Specht and Jian Shi and J\'{e}r\^{o}me Querard and Buyan Pan and Julien Rossignol and Karine Moncoq and Nelly Morellet and Michel Volovitch and Ewen Lescop and Yong Chen and Antoine Triller and Sophie Vriz and Thomas Le Saux and Ludovic Jullien and Arnaud Gautier}, doi = {10.1073/pnas.1513094113}, year = {2016}, date = {2016-01-01}, journal = {Proceedings of the National Academy of Sciences}, volume = {113}, number = {3}, pages = {497}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2015 |
Control of brain patterning by engrailed paracrine transfer: A new function of the pbx interaction domain Article de journal C Rampon; C Gauron; T Lin; F Meda; E Dupont; A Cosson; E Ipendey; A Frerot; I Aujard; T Le Saux; D Bensimon; L Jullien; M Volovitch; S Vriz; A Joliot Development (Cambridge), 142 (10), p. 1840–1849, 2015. @article{Rampon:2015, title = {Control of brain patterning by engrailed paracrine transfer: A new function of the pbx interaction domain}, author = {C Rampon and C Gauron and T Lin and F Meda and E Dupont and A Cosson and E Ipendey and A Frerot and I Aujard and T Le Saux and D Bensimon and L Jullien and M Volovitch and S Vriz and A Joliot}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84929206944&doi=10.1242%2fdev.114181&partnerID=40&md5=016ff1da90c2292976a12fee82b3ed59}, doi = {10.1242/dev.114181}, year = {2015}, date = {2015-01-01}, journal = {Development (Cambridge)}, volume = {142}, number = {10}, pages = {1840--1849}, abstract = {Homeoproteins of the Engrailed family are involved in the patterning of mesencephalic boundaries through a mechanism classically ascribed to their transcriptional functions. In light of recent reports on the paracrine activity of homeoproteins, including Engrailed, we asked whether Engrailed intercellular transfer was also involved in brain patterning and boundary formation. Using time-controlled activation of Engrailed combined with tools that block its transfer, we show that the positioning of the diencephalic-mesencephalic boundary (DMB) requires Engrailed paracrine activity. Both zebrafish Eng2a and Eng2b are competent for intercellular transfer in vivo, but only extracellular endogenous Eng2b, and not Eng2a, participates in DMB positioning. In addition, disruption of the Pbx-interacting motif in Engrailed, known to strongly reduce the gain-of-function phenotype, also downregulates Engrailed transfer, thus revealing an unsuspected participation of the Pbx interaction domain in this pathway. © 2015. Published by The Company of Biologists Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Homeoproteins of the Engrailed family are involved in the patterning of mesencephalic boundaries through a mechanism classically ascribed to their transcriptional functions. In light of recent reports on the paracrine activity of homeoproteins, including Engrailed, we asked whether Engrailed intercellular transfer was also involved in brain patterning and boundary formation. Using time-controlled activation of Engrailed combined with tools that block its transfer, we show that the positioning of the diencephalic-mesencephalic boundary (DMB) requires Engrailed paracrine activity. Both zebrafish Eng2a and Eng2b are competent for intercellular transfer in vivo, but only extracellular endogenous Eng2b, and not Eng2a, participates in DMB positioning. In addition, disruption of the Pbx-interacting motif in Engrailed, known to strongly reduce the gain-of-function phenotype, also downregulates Engrailed transfer, thus revealing an unsuspected participation of the Pbx interaction domain in this pathway. © 2015. Published by The Company of Biologists Ltd. |
Expanding discriminative dimensions for analysis and imaging Article de journal J Querard; A Gautier; T Le Saux; L Jullien Chemical Science, 6 (5), p. 2968–2978, 2015. @article{Querard:2015, title = {Expanding discriminative dimensions for analysis and imaging}, author = {J Querard and A Gautier and T Le Saux and L Jullien}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84928139717&doi=10.1039%2fc4sc03955f&partnerID=40&md5=aeed36c64e258429fa7a7aa11b8baba8}, doi = {10.1039/c4sc03955f}, year = {2015}, date = {2015-01-01}, journal = {Chemical Science}, volume = {6}, number = {5}, pages = {2968--2978}, abstract = {Eliminating the contribution of interfering compounds is a key step in chemical analysis. In complex media, one possible approach is to perform a preliminary separation. However purification is often demanding, long, and costly; it may also considerably alter the properties of interacting components of the mixture (e.g. in a living cell). Hence there is a strong interest for developing separation-free non-invasive analytical protocols. Using photoswitchable probes as labelling and titration contrast agents, we demonstrate that the association of a modulated monochromatic light excitation with a kinetic filtering of the overall observable is much more attractive than constant excitation to read-out the contribution from a target probe under adverse conditions. An extensive theoretical framework enabled us to optimize the out-of-phase concentration first-order response of a photoswitchable probe to modulated illumination by appropriately matching the average light intensity and the radial frequency of the light modulation to the probe dynamics. Thus, we can selectively and quantitatively extract from an overall signal the contribution from a target photoswitchable probe within a mixture of species, photoswitchable or not. This simple titration strategy is more specifically developed in the context of fluorescence imaging, which offers promising perspectives. © The Royal Society of Chemistry 2015.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Eliminating the contribution of interfering compounds is a key step in chemical analysis. In complex media, one possible approach is to perform a preliminary separation. However purification is often demanding, long, and costly; it may also considerably alter the properties of interacting components of the mixture (e.g. in a living cell). Hence there is a strong interest for developing separation-free non-invasive analytical protocols. Using photoswitchable probes as labelling and titration contrast agents, we demonstrate that the association of a modulated monochromatic light excitation with a kinetic filtering of the overall observable is much more attractive than constant excitation to read-out the contribution from a target probe under adverse conditions. An extensive theoretical framework enabled us to optimize the out-of-phase concentration first-order response of a photoswitchable probe to modulated illumination by appropriately matching the average light intensity and the radial frequency of the light modulation to the probe dynamics. Thus, we can selectively and quantitatively extract from an overall signal the contribution from a target photoswitchable probe within a mixture of species, photoswitchable or not. This simple titration strategy is more specifically developed in the context of fluorescence imaging, which offers promising perspectives. © The Royal Society of Chemistry 2015. |
Fluorogen-Based Reporters for Fluorescence Imaging: A Review Article de journal Ludovic Jullien; Arnaud Gautier Methods and Applications in Fluorescence, 3 (4), p. 042007, 2015, ISSN: 2050-6120. @article{RN38, title = {Fluorogen-Based Reporters for Fluorescence Imaging: A Review}, author = {Ludovic Jullien and Arnaud Gautier}, doi = {10.1088/2050-6120/3/4/042007}, issn = {2050-6120}, year = {2015}, date = {2015-01-01}, journal = {Methods and Applications in Fluorescence}, volume = {3}, number = {4}, pages = {042007}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Light-Activated Proteolysis for the Spatiotemporal Control of Proteins Article de journal Quentin Delacour; Chenge Li; Marie-Aude Plamont; Emmanuelle Billon-Denis; Isabelle Aujard; Thomas Le Saux; Ludovic Jullien; Arnaud Gautier ACS Chemical Biology, 10 (7), p. 1643-1647, 2015, ISSN: 1554-8929. @article{RN37, title = {Light-Activated Proteolysis for the Spatiotemporal Control of Proteins}, author = {Quentin Delacour and Chenge Li and Marie-Aude Plamont and Emmanuelle {Billon-Denis} and Isabelle Aujard and Thomas Le Saux and Ludovic Jullien and Arnaud Gautier}, doi = {10.1021/acschembio.5b00069}, issn = {1554-8929}, year = {2015}, date = {2015-01-01}, journal = {ACS Chemical Biology}, volume = {10}, number = {7}, pages = {1643-1647}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Photoswitching Kinetics and Phase-Sensitive Detection Add Discriminative Dimensions for Selective Fluorescence Imaging Article de journal Jérôme Querard; Tal-Zvi Markus; Marie-Aude Plamont; Carole Gauron; Pengcheng Wang; Agathe Espagne; Michel Volovitch; Sophie Vriz; Vincent Croquette; Arnaud Gautier; Thomas Le Saux; Ludovic Jullien Angewandte Chemie International Edition, 54 (9), p. 2633-2637, 2015, ISSN: 1433-7851. @article{RN43b, title = {Photoswitching Kinetics and Phase-Sensitive Detection Add Discriminative Dimensions for Selective Fluorescence Imaging}, author = {J\'{e}r\^{o}me Querard and Tal-Zvi Markus and Marie-Aude Plamont and Carole Gauron and Pengcheng Wang and Agathe Espagne and Michel Volovitch and Sophie Vriz and Vincent Croquette and Arnaud Gautier and Thomas Le Saux and Ludovic Jullien}, doi = {10.1002/anie.201408985}, issn = {1433-7851}, year = {2015}, date = {2015-01-01}, journal = {Angewandte Chemie International Edition}, volume = {54}, number = {9}, pages = {2633-2637}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2014 |
Disassembly kinetics of quinone-methide-based self-immolative spacers that contain aromatic nitrogen heterocycles Article de journal A Alouane; R Labruère; K J Silvestre; T Le Saux; F Schmidt; L Jullien Chemistry - An Asian Journal, 9 (5), p. 1334–1340, 2014. @article{Alouane:2014, title = {Disassembly kinetics of quinone-methide-based self-immolative spacers that contain aromatic nitrogen heterocycles}, author = {A Alouane and R Labru\`{e}re and K J Silvestre and T Le Saux and F Schmidt and L Jullien}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84899466220&doi=10.1002%2fasia.201400051&partnerID=40&md5=a330e468d911417e1ffcc3eae297f08a}, doi = {10.1002/asia.201400051}, year = {2014}, date = {2014-01-01}, journal = {Chemistry - An Asian Journal}, volume = {9}, number = {5}, pages = {1334--1340}, abstract = {We prepared several pyridine- and pyrimidine-based self-immolative spacer groups to evaluate the significance of the resonance energy of the spacer aromatic ring on the kinetics of 1,4- and 1,6-elimination reactions, which govern spacer disassembly. Subsequently, we relied on a photoactivation procedure to accurately analyze the disassembly kinetics. Beyond providing new results that are relevant for deriving quantitative structure-property relationships, herein, we demonstrate that pH value can be used as an efficient parameter to finely control the disassembly time of a self-immolative spacer after an initial activation. Burn rubber: Kinetic analysis of the pH-dependent disassembly of self-immolative spacers that contain aromatic nitrogen heterocycles was performed. Electron-poor pyrimidine cores exhibited the longest disassembly times. This study confirms the trend that electron-rich aryl cores accelerate self-immolation. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We prepared several pyridine- and pyrimidine-based self-immolative spacer groups to evaluate the significance of the resonance energy of the spacer aromatic ring on the kinetics of 1,4- and 1,6-elimination reactions, which govern spacer disassembly. Subsequently, we relied on a photoactivation procedure to accurately analyze the disassembly kinetics. Beyond providing new results that are relevant for deriving quantitative structure-property relationships, herein, we demonstrate that pH value can be used as an efficient parameter to finely control the disassembly time of a self-immolative spacer after an initial activation. Burn rubber: Kinetic analysis of the pH-dependent disassembly of self-immolative spacers that contain aromatic nitrogen heterocycles was performed. Electron-poor pyrimidine cores exhibited the longest disassembly times. This study confirms the trend that electron-rich aryl cores accelerate self-immolation. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
How to Control Proteins with Light in Living Systems Article de journal Arnaud Gautier; Carole Gauron; Michel Volovitch; David Bensimon; Ludovic Jullien; Sophie Vriz Nature Chemical Biology, 10 , p. 533, 2014. @article{RN42, title = {How to Control Proteins with Light in Living Systems}, author = {Arnaud Gautier and Carole Gauron and Michel Volovitch and David Bensimon and Ludovic Jullien and Sophie Vriz}, doi = {10.1038/nchembio.1534}, year = {2014}, date = {2014-01-01}, journal = {Nature Chemical Biology}, volume = {10}, pages = {533}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
How to prepare the minds at best? Article de journal L Jullien Actualite Chimique, (385), p. 15–17, 2014. @article{Jullien:2014, title = {How to prepare the minds at best?}, author = {L Jullien}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84901981303&partnerID=40&md5=663a293b401b02e84bb6994e38140f46}, year = {2014}, date = {2014-01-01}, journal = {Actualite Chimique}, number = {385}, pages = {15--17}, abstract = {How to prepare the minds at best? Each teacher has to conceive and implement her/his course. This article reports on the experience of a professor of chemistry, who significantly has devoted his time and his thoughts to this exercise upon constantly aiming at educating, emancipating, and freeing young pupils as well as confirmed students.}, keywords = {}, pubstate = {published}, tppubtype = {article} } How to prepare the minds at best? Each teacher has to conceive and implement her/his course. This article reports on the experience of a professor of chemistry, who significantly has devoted his time and his thoughts to this exercise upon constantly aiming at educating, emancipating, and freeing young pupils as well as confirmed students. |