Professeur des Universités, Sorbonne Université
Responsable du pôle de Chimie Physique et Biologique de la Matière Vivante
PASTEUR, Département de chimie, École Normale Supérieure, PSL University, Sorbonne Université, CNRS
24 rue Lhomond, 75005 Paris, France
Email: Ludovic.Jullien@ens.psl.eu or Ludovic.Jullien@sorbonne-universite.fr
Phone: +33 144323333
Office: E142c
Home page: https://ludovicjullien.org/
Publications
2017 |
Syntheses and kinetic studies of cyclisation-based self-immolative spacers Article de journal S Huvelle; A Alouane; T Le Saux; L Jullien; F Schmidt Organic and Biomolecular Chemistry, 15 (16), p. 3435–3443, 2017. @article{Huvelle:2017, title = {Syntheses and kinetic studies of cyclisation-based self-immolative spacers}, author = {S Huvelle and A Alouane and T Le Saux and L Jullien and F Schmidt}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85017654758&doi=10.1039%2fc7ob00121e&partnerID=40&md5=b3efbccb7d9af044089b4be18ab3b100}, doi = {10.1039/c7ob00121e}, year = {2017}, date = {2017-01-01}, journal = {Organic and Biomolecular Chemistry}, volume = {15}, number = {16}, pages = {3435--3443}, abstract = {Kinetic analysis of the disassembly of self-immolative spacers based on cyclisation processes was performed. Five compounds were synthesized belonging to two different series, and their kinetic constants were determined. Electron-donating substituents gave a slight acceleration but the main effect was steric, and the Thorpe-Ingold effect was indeed particularly effective. Comparison with the self-immolative spacers based on elimination processes showed that cyclisations gave comparable or lower rate, but the corresponding spacers are more difficult to modulate. © The Royal Society of Chemistry.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Kinetic analysis of the disassembly of self-immolative spacers based on cyclisation processes was performed. Five compounds were synthesized belonging to two different series, and their kinetic constants were determined. Electron-donating substituents gave a slight acceleration but the main effect was steric, and the Thorpe-Ingold effect was indeed particularly effective. Comparison with the self-immolative spacers based on elimination processes showed that cyclisations gave comparable or lower rate, but the corresponding spacers are more difficult to modulate. © The Royal Society of Chemistry. |
2016 |
Design and characterization of red fluorogenic push-pull chromophores holding great potential for bioimaging and biosensing Article de journal C Li; M -A Plamont; I Aujard; T Le Saux; L Jullien; A Gautier Organic and Biomolecular Chemistry, 14 (39), p. 9253–9261, 2016. @article{Li:2016, title = {Design and characterization of red fluorogenic push-pull chromophores holding great potential for bioimaging and biosensing}, author = {C Li and M -A Plamont and I Aujard and T Le Saux and L Jullien and A Gautier}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84990182453&doi=10.1039%2fc6ob01612j&partnerID=40&md5=7cea2c6d4aeeeef91e6a3a5bd0d6b5ad}, doi = {10.1039/c6ob01612j}, year = {2016}, date = {2016-01-01}, journal = {Organic and Biomolecular Chemistry}, volume = {14}, number = {39}, pages = {9253--9261}, abstract = {Fluorogenic chromophores have been used recently for fluorescence reporting and biosensing. Their ability to turn on upon specific interaction with a given target has been exploited in particular for the design of fluorogen-based reporters enabling biomolecule labeling and imaging. In this paper, we report the development and exhaustive characterization of a new family of red fluorogenic push-pull chromophores, holding great potential for the development of fluorogen-based reporters or intracellular fluorogenic markers. The proposed methodology is generic and should find general applicability in the discovery of new fluorogenic dyes suitable for the design of fluorogen-based reporters and biosensors. © The Royal Society of Chemistry 2016.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Fluorogenic chromophores have been used recently for fluorescence reporting and biosensing. Their ability to turn on upon specific interaction with a given target has been exploited in particular for the design of fluorogen-based reporters enabling biomolecule labeling and imaging. In this paper, we report the development and exhaustive characterization of a new family of red fluorogenic push-pull chromophores, holding great potential for the development of fluorogen-based reporters or intracellular fluorogenic markers. The proposed methodology is generic and should find general applicability in the discovery of new fluorogenic dyes suitable for the design of fluorogen-based reporters and biosensors. © The Royal Society of Chemistry 2016. |
Kinetics of Reactive Modules Adds Discriminative Dimensions for Selective Cell Imaging Article de journal J Quérard; T Le Saux; A Gautier; D Alcor; V Croquette; A Lemarchand; C Gosse; L Jullien ChemPhysChem, 17 (10), p. 1396–1413, 2016. @article{Querard:2016, title = {Kinetics of Reactive Modules Adds Discriminative Dimensions for Selective Cell Imaging}, author = {J Qu\'{e}rard and T Le Saux and A Gautier and D Alcor and V Croquette and A Lemarchand and C Gosse and L Jullien}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84957539317&doi=10.1002%2fcphc.201500987&partnerID=40&md5=4a514250af842ae7697b695eb73769fb}, doi = {10.1002/cphc.201500987}, year = {2016}, date = {2016-01-01}, journal = {ChemPhysChem}, volume = {17}, number = {10}, pages = {1396--1413}, abstract = {Living cells are chemical mixtures of exceptional interest and significance, whose investigation requires the development of powerful analytical tools fulfilling the demanding constraints resulting from their singular features. In particular, multiplexed observation of a large number of molecular targets with high spatiotemporal resolution appears highly desirable. One attractive road to address this analytical challenge relies on engaging the targets in reactions and exploiting the rich kinetic signature of the resulting reactive module, which originates from its topology and its rate constants. This review explores the various facets of this promising strategy. We first emphasize the singularity of the content of a living cell as a chemical mixture and suggest that its multiplexed observation is significant and timely. Then, we show that exploiting the kinetics of analytical processes is relevant to selectively detect a given analyte: upon perturbing the system, the kinetic window associated to response read-out has to be matched with that of the targeted reactive module. Eventually, we introduce the state-of-the-art of cell imaging exploiting protocols based on reaction kinetics and draw some promising perspectives. Live recording: In living cells, the multiplexed observation of a large number of targets has recently emerged as a desirable goal. To address this challenge, an attractive road relies on engaging the targets in reactions and exploiting the kinetic signature of the resulting reactive module. This review explores the facets of this strategy and draw some promising perspectives. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Living cells are chemical mixtures of exceptional interest and significance, whose investigation requires the development of powerful analytical tools fulfilling the demanding constraints resulting from their singular features. In particular, multiplexed observation of a large number of molecular targets with high spatiotemporal resolution appears highly desirable. One attractive road to address this analytical challenge relies on engaging the targets in reactions and exploiting the rich kinetic signature of the resulting reactive module, which originates from its topology and its rate constants. This review explores the various facets of this promising strategy. We first emphasize the singularity of the content of a living cell as a chemical mixture and suggest that its multiplexed observation is significant and timely. Then, we show that exploiting the kinetics of analytical processes is relevant to selectively detect a given analyte: upon perturbing the system, the kinetic window associated to response read-out has to be matched with that of the targeted reactive module. Eventually, we introduce the state-of-the-art of cell imaging exploiting protocols based on reaction kinetics and draw some promising perspectives. Live recording: In living cells, the multiplexed observation of a large number of targets has recently emerged as a desirable goal. To address this challenge, an attractive road relies on engaging the targets in reactions and exploiting the kinetic signature of the resulting reactive module. This review explores the facets of this strategy and draw some promising perspectives. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Small Fluorescence-Activating and Absorption-Shifting Tag for Tunable Protein Imaging in Vivo Article de journal Marie-Aude Plamont; Emmanuelle Billon-Denis; Sylvie Maurin; Carole Gauron; Frederico M Pimenta; Christian G Specht; Jian Shi; Jérôme Querard; Buyan Pan; Julien Rossignol; Karine Moncoq; Nelly Morellet; Michel Volovitch; Ewen Lescop; Yong Chen; Antoine Triller; Sophie Vriz; Thomas Le Saux; Ludovic Jullien; Arnaud Gautier Proceedings of the National Academy of Sciences, 113 (3), p. 497, 2016. @article{RN50, title = {Small Fluorescence-Activating and Absorption-Shifting Tag for Tunable Protein Imaging in Vivo}, author = {Marie-Aude Plamont and Emmanuelle {Billon-Denis} and Sylvie Maurin and Carole Gauron and Frederico M Pimenta and Christian G Specht and Jian Shi and J\'{e}r\^{o}me Querard and Buyan Pan and Julien Rossignol and Karine Moncoq and Nelly Morellet and Michel Volovitch and Ewen Lescop and Yong Chen and Antoine Triller and Sophie Vriz and Thomas Le Saux and Ludovic Jullien and Arnaud Gautier}, doi = {10.1073/pnas.1513094113}, year = {2016}, date = {2016-01-01}, journal = {Proceedings of the National Academy of Sciences}, volume = {113}, number = {3}, pages = {497}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2015 |
Control of brain patterning by engrailed paracrine transfer: A new function of the pbx interaction domain Article de journal C Rampon; C Gauron; T Lin; F Meda; E Dupont; A Cosson; E Ipendey; A Frerot; I Aujard; T Le Saux; D Bensimon; L Jullien; M Volovitch; S Vriz; A Joliot Development (Cambridge), 142 (10), p. 1840–1849, 2015. @article{Rampon:2015, title = {Control of brain patterning by engrailed paracrine transfer: A new function of the pbx interaction domain}, author = {C Rampon and C Gauron and T Lin and F Meda and E Dupont and A Cosson and E Ipendey and A Frerot and I Aujard and T Le Saux and D Bensimon and L Jullien and M Volovitch and S Vriz and A Joliot}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84929206944&doi=10.1242%2fdev.114181&partnerID=40&md5=016ff1da90c2292976a12fee82b3ed59}, doi = {10.1242/dev.114181}, year = {2015}, date = {2015-01-01}, journal = {Development (Cambridge)}, volume = {142}, number = {10}, pages = {1840--1849}, abstract = {Homeoproteins of the Engrailed family are involved in the patterning of mesencephalic boundaries through a mechanism classically ascribed to their transcriptional functions. In light of recent reports on the paracrine activity of homeoproteins, including Engrailed, we asked whether Engrailed intercellular transfer was also involved in brain patterning and boundary formation. Using time-controlled activation of Engrailed combined with tools that block its transfer, we show that the positioning of the diencephalic-mesencephalic boundary (DMB) requires Engrailed paracrine activity. Both zebrafish Eng2a and Eng2b are competent for intercellular transfer in vivo, but only extracellular endogenous Eng2b, and not Eng2a, participates in DMB positioning. In addition, disruption of the Pbx-interacting motif in Engrailed, known to strongly reduce the gain-of-function phenotype, also downregulates Engrailed transfer, thus revealing an unsuspected participation of the Pbx interaction domain in this pathway. © 2015. Published by The Company of Biologists Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Homeoproteins of the Engrailed family are involved in the patterning of mesencephalic boundaries through a mechanism classically ascribed to their transcriptional functions. In light of recent reports on the paracrine activity of homeoproteins, including Engrailed, we asked whether Engrailed intercellular transfer was also involved in brain patterning and boundary formation. Using time-controlled activation of Engrailed combined with tools that block its transfer, we show that the positioning of the diencephalic-mesencephalic boundary (DMB) requires Engrailed paracrine activity. Both zebrafish Eng2a and Eng2b are competent for intercellular transfer in vivo, but only extracellular endogenous Eng2b, and not Eng2a, participates in DMB positioning. In addition, disruption of the Pbx-interacting motif in Engrailed, known to strongly reduce the gain-of-function phenotype, also downregulates Engrailed transfer, thus revealing an unsuspected participation of the Pbx interaction domain in this pathway. © 2015. Published by The Company of Biologists Ltd. |
Expanding discriminative dimensions for analysis and imaging Article de journal J Querard; A Gautier; T Le Saux; L Jullien Chemical Science, 6 (5), p. 2968–2978, 2015. @article{Querard:2015, title = {Expanding discriminative dimensions for analysis and imaging}, author = {J Querard and A Gautier and T Le Saux and L Jullien}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84928139717&doi=10.1039%2fc4sc03955f&partnerID=40&md5=aeed36c64e258429fa7a7aa11b8baba8}, doi = {10.1039/c4sc03955f}, year = {2015}, date = {2015-01-01}, journal = {Chemical Science}, volume = {6}, number = {5}, pages = {2968--2978}, abstract = {Eliminating the contribution of interfering compounds is a key step in chemical analysis. In complex media, one possible approach is to perform a preliminary separation. However purification is often demanding, long, and costly; it may also considerably alter the properties of interacting components of the mixture (e.g. in a living cell). Hence there is a strong interest for developing separation-free non-invasive analytical protocols. Using photoswitchable probes as labelling and titration contrast agents, we demonstrate that the association of a modulated monochromatic light excitation with a kinetic filtering of the overall observable is much more attractive than constant excitation to read-out the contribution from a target probe under adverse conditions. An extensive theoretical framework enabled us to optimize the out-of-phase concentration first-order response of a photoswitchable probe to modulated illumination by appropriately matching the average light intensity and the radial frequency of the light modulation to the probe dynamics. Thus, we can selectively and quantitatively extract from an overall signal the contribution from a target photoswitchable probe within a mixture of species, photoswitchable or not. This simple titration strategy is more specifically developed in the context of fluorescence imaging, which offers promising perspectives. © The Royal Society of Chemistry 2015.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Eliminating the contribution of interfering compounds is a key step in chemical analysis. In complex media, one possible approach is to perform a preliminary separation. However purification is often demanding, long, and costly; it may also considerably alter the properties of interacting components of the mixture (e.g. in a living cell). Hence there is a strong interest for developing separation-free non-invasive analytical protocols. Using photoswitchable probes as labelling and titration contrast agents, we demonstrate that the association of a modulated monochromatic light excitation with a kinetic filtering of the overall observable is much more attractive than constant excitation to read-out the contribution from a target probe under adverse conditions. An extensive theoretical framework enabled us to optimize the out-of-phase concentration first-order response of a photoswitchable probe to modulated illumination by appropriately matching the average light intensity and the radial frequency of the light modulation to the probe dynamics. Thus, we can selectively and quantitatively extract from an overall signal the contribution from a target photoswitchable probe within a mixture of species, photoswitchable or not. This simple titration strategy is more specifically developed in the context of fluorescence imaging, which offers promising perspectives. © The Royal Society of Chemistry 2015. |
Fluorogen-Based Reporters for Fluorescence Imaging: A Review Article de journal Ludovic Jullien; Arnaud Gautier Methods and Applications in Fluorescence, 3 (4), p. 042007, 2015, ISSN: 2050-6120. @article{RN38, title = {Fluorogen-Based Reporters for Fluorescence Imaging: A Review}, author = {Ludovic Jullien and Arnaud Gautier}, doi = {10.1088/2050-6120/3/4/042007}, issn = {2050-6120}, year = {2015}, date = {2015-01-01}, journal = {Methods and Applications in Fluorescence}, volume = {3}, number = {4}, pages = {042007}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Light-Activated Proteolysis for the Spatiotemporal Control of Proteins Article de journal Quentin Delacour; Chenge Li; Marie-Aude Plamont; Emmanuelle Billon-Denis; Isabelle Aujard; Thomas Le Saux; Ludovic Jullien; Arnaud Gautier ACS Chemical Biology, 10 (7), p. 1643-1647, 2015, ISSN: 1554-8929. @article{RN37, title = {Light-Activated Proteolysis for the Spatiotemporal Control of Proteins}, author = {Quentin Delacour and Chenge Li and Marie-Aude Plamont and Emmanuelle {Billon-Denis} and Isabelle Aujard and Thomas Le Saux and Ludovic Jullien and Arnaud Gautier}, doi = {10.1021/acschembio.5b00069}, issn = {1554-8929}, year = {2015}, date = {2015-01-01}, journal = {ACS Chemical Biology}, volume = {10}, number = {7}, pages = {1643-1647}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Photoswitching Kinetics and Phase-Sensitive Detection Add Discriminative Dimensions for Selective Fluorescence Imaging Article de journal Jérôme Querard; Tal-Zvi Markus; Marie-Aude Plamont; Carole Gauron; Pengcheng Wang; Agathe Espagne; Michel Volovitch; Sophie Vriz; Vincent Croquette; Arnaud Gautier; Thomas Le Saux; Ludovic Jullien Angewandte Chemie International Edition, 54 (9), p. 2633-2637, 2015, ISSN: 1433-7851. @article{RN43b, title = {Photoswitching Kinetics and Phase-Sensitive Detection Add Discriminative Dimensions for Selective Fluorescence Imaging}, author = {J\'{e}r\^{o}me Querard and Tal-Zvi Markus and Marie-Aude Plamont and Carole Gauron and Pengcheng Wang and Agathe Espagne and Michel Volovitch and Sophie Vriz and Vincent Croquette and Arnaud Gautier and Thomas Le Saux and Ludovic Jullien}, doi = {10.1002/anie.201408985}, issn = {1433-7851}, year = {2015}, date = {2015-01-01}, journal = {Angewandte Chemie International Edition}, volume = {54}, number = {9}, pages = {2633-2637}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2014 |
Disassembly kinetics of quinone-methide-based self-immolative spacers that contain aromatic nitrogen heterocycles Article de journal A Alouane; R Labruère; K J Silvestre; T Le Saux; F Schmidt; L Jullien Chemistry - An Asian Journal, 9 (5), p. 1334–1340, 2014. @article{Alouane:2014, title = {Disassembly kinetics of quinone-methide-based self-immolative spacers that contain aromatic nitrogen heterocycles}, author = {A Alouane and R Labru\`{e}re and K J Silvestre and T Le Saux and F Schmidt and L Jullien}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84899466220&doi=10.1002%2fasia.201400051&partnerID=40&md5=a330e468d911417e1ffcc3eae297f08a}, doi = {10.1002/asia.201400051}, year = {2014}, date = {2014-01-01}, journal = {Chemistry - An Asian Journal}, volume = {9}, number = {5}, pages = {1334--1340}, abstract = {We prepared several pyridine- and pyrimidine-based self-immolative spacer groups to evaluate the significance of the resonance energy of the spacer aromatic ring on the kinetics of 1,4- and 1,6-elimination reactions, which govern spacer disassembly. Subsequently, we relied on a photoactivation procedure to accurately analyze the disassembly kinetics. Beyond providing new results that are relevant for deriving quantitative structure-property relationships, herein, we demonstrate that pH value can be used as an efficient parameter to finely control the disassembly time of a self-immolative spacer after an initial activation. Burn rubber: Kinetic analysis of the pH-dependent disassembly of self-immolative spacers that contain aromatic nitrogen heterocycles was performed. Electron-poor pyrimidine cores exhibited the longest disassembly times. This study confirms the trend that electron-rich aryl cores accelerate self-immolation. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We prepared several pyridine- and pyrimidine-based self-immolative spacer groups to evaluate the significance of the resonance energy of the spacer aromatic ring on the kinetics of 1,4- and 1,6-elimination reactions, which govern spacer disassembly. Subsequently, we relied on a photoactivation procedure to accurately analyze the disassembly kinetics. Beyond providing new results that are relevant for deriving quantitative structure-property relationships, herein, we demonstrate that pH value can be used as an efficient parameter to finely control the disassembly time of a self-immolative spacer after an initial activation. Burn rubber: Kinetic analysis of the pH-dependent disassembly of self-immolative spacers that contain aromatic nitrogen heterocycles was performed. Electron-poor pyrimidine cores exhibited the longest disassembly times. This study confirms the trend that electron-rich aryl cores accelerate self-immolation. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
How to Control Proteins with Light in Living Systems Article de journal Arnaud Gautier; Carole Gauron; Michel Volovitch; David Bensimon; Ludovic Jullien; Sophie Vriz Nature Chemical Biology, 10 , p. 533, 2014. @article{RN42, title = {How to Control Proteins with Light in Living Systems}, author = {Arnaud Gautier and Carole Gauron and Michel Volovitch and David Bensimon and Ludovic Jullien and Sophie Vriz}, doi = {10.1038/nchembio.1534}, year = {2014}, date = {2014-01-01}, journal = {Nature Chemical Biology}, volume = {10}, pages = {533}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
How to prepare the minds at best? Article de journal L Jullien Actualite Chimique, (385), p. 15–17, 2014. @article{Jullien:2014, title = {How to prepare the minds at best?}, author = {L Jullien}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84901981303&partnerID=40&md5=663a293b401b02e84bb6994e38140f46}, year = {2014}, date = {2014-01-01}, journal = {Actualite Chimique}, number = {385}, pages = {15--17}, abstract = {How to prepare the minds at best? Each teacher has to conceive and implement her/his course. This article reports on the experience of a professor of chemistry, who significantly has devoted his time and his thoughts to this exercise upon constantly aiming at educating, emancipating, and freeing young pupils as well as confirmed students.}, keywords = {}, pubstate = {published}, tppubtype = {article} } How to prepare the minds at best? Each teacher has to conceive and implement her/his course. This article reports on the experience of a professor of chemistry, who significantly has devoted his time and his thoughts to this exercise upon constantly aiming at educating, emancipating, and freeing young pupils as well as confirmed students. |
Photocontrolled ionization in the corona of rodlike assemblies of diblock copolymers Article de journal J Sun; L Jia; M Emond; M -H Li; E Marie; L Jullien; C Tribet Macromolecules, 47 (5), p. 1684–1692, 2014. @article{Sun:2014, title = {Photocontrolled ionization in the corona of rodlike assemblies of diblock copolymers}, author = {J Sun and L Jia and M Emond and M -H Li and E Marie and L Jullien and C Tribet}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84900623616&doi=10.1021%2fma402591y&partnerID=40&md5=096eeaa435f309dc54c178b236d1de7c}, doi = {10.1021/ma402591y}, year = {2014}, date = {2014-01-01}, journal = {Macromolecules}, volume = {47}, number = {5}, pages = {1684--1692}, abstract = {To remotely control ionization of polymer assemblies, we tailored amphiphilic diblock polyacrylates with varying hydrophilic and hydrophobic block lengths and containing pendant 2-hydroxyazobenzene photoswitchable groups in the hydrophilic block. Formation in water of rodlike polymer micelles was obtained upon hydrophobic assembly of the pendant cholesterol groups present in the hydrophobic block. Phototriggered variation of both pH and UV-vis spectral properties confirmed that hydroxylazobenzene moieties, gathered in the hydrophilic corona, underwent both isomerization and ionization upon exposure to UV light. Dispersions of rods can accordingly be ionized on demand. © 2014 American Chemical Society.}, keywords = {}, pubstate = {published}, tppubtype = {article} } To remotely control ionization of polymer assemblies, we tailored amphiphilic diblock polyacrylates with varying hydrophilic and hydrophobic block lengths and containing pendant 2-hydroxyazobenzene photoswitchable groups in the hydrophilic block. Formation in water of rodlike polymer micelles was obtained upon hydrophobic assembly of the pendant cholesterol groups present in the hydrophobic block. Phototriggered variation of both pH and UV-vis spectral properties confirmed that hydroxylazobenzene moieties, gathered in the hydrophilic corona, underwent both isomerization and ionization upon exposure to UV light. Dispersions of rods can accordingly be ionized on demand. © 2014 American Chemical Society. |
Rapidly tunable and compact coherent Raman scattering light source for molecular spectroscopy Article de journal S Saint-Jalm; P Berto; L Jullien; E R Andresen; H Rigneault Journal of Raman Spectroscopy, 45 (7), p. 515–520, 2014. @article{Saint-Jalm:2014, title = {Rapidly tunable and compact coherent Raman scattering light source for molecular spectroscopy}, author = {S Saint-Jalm and P Berto and L Jullien and E R Andresen and H Rigneault}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84904215402&doi=10.1002%2fjrs.4514&partnerID=40&md5=4cb0181caada13d4877cdcb5ab9d00f8}, doi = {10.1002/jrs.4514}, year = {2014}, date = {2014-01-01}, journal = {Journal of Raman Spectroscopy}, volume = {45}, number = {7}, pages = {515--520}, abstract = {We present a rapidly tunable (over 400 cm -1) and compact (0.7 m 2 footprint) coherent Raman scattering light source performing both coherent anti-Stokes Raman scattering and stimulated Raman scattering microspectroscopy. We use spectral focusing of a femtosecond Ti:Sapphire pulse and a redshifted soliton generated in a photonic crystal fiber to reach suitable spectral resolution and to rapidly acquire spectra by means of a delay line translation. The coherent Raman scattering light source is used to monitor the molecular equilibrium shift between hydrogen phosphate and dihydrogen phosphate ions under pH change. Copyright © 2014 John Wiley & Sons, Ltd.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We present a rapidly tunable (over 400 cm -1) and compact (0.7 m 2 footprint) coherent Raman scattering light source performing both coherent anti-Stokes Raman scattering and stimulated Raman scattering microspectroscopy. We use spectral focusing of a femtosecond Ti:Sapphire pulse and a redshifted soliton generated in a photonic crystal fiber to reach suitable spectral resolution and to rapidly acquire spectra by means of a delay line translation. The coherent Raman scattering light source is used to monitor the molecular equilibrium shift between hydrogen phosphate and dihydrogen phosphate ions under pH change. Copyright © 2014 John Wiley & Sons, Ltd. |
2013 |
A blue-absorbing photolabile protecting group for in vivo chromatically orthogonal photoactivation Article de journal L Fournier; C Gauron; L Xu; I Aujard; T Le Saux; N Gagey-Eilstein; S Maurin; S Dubruille; J -B Baudin; D Bensimon; M Volovitch; S Vriz; L Jullien ACS Chemical Biology, 8 (7), p. 1528–1536, 2013. @article{Fournier:2013a, title = {A blue-absorbing photolabile protecting group for in vivo chromatically orthogonal photoactivation}, author = {L Fournier and C Gauron and L Xu and I Aujard and T Le Saux and N Gagey-Eilstein and S Maurin and S Dubruille and J -B Baudin and D Bensimon and M Volovitch and S Vriz and L Jullien}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84880534465&doi=10.1021%2fcb400178m&partnerID=40&md5=a6d82b0b12d74445d9235b43207903ed}, doi = {10.1021/cb400178m}, year = {2013}, date = {2013-01-01}, journal = {ACS Chemical Biology}, volume = {8}, number = {7}, pages = {1528--1536}, abstract = {The small and synthetically easily accessible 7-diethylamino-4- thiocoumarinylmethyl photolabile protecting group has been validated for uncaging with blue light. It exhibits a significant action cross-section for uncaging in the 470-500 nm wavelength range and a low light absorption between 350 and 400 nm. These attractive features have been implemented in living zebrafish embryos to perform chromatic orthogonal photoactivation of two biologically active species controlling biological development with UV and blue-cyan light sources, respectively. © 2013 American Chemical Society.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The small and synthetically easily accessible 7-diethylamino-4- thiocoumarinylmethyl photolabile protecting group has been validated for uncaging with blue light. It exhibits a significant action cross-section for uncaging in the 470-500 nm wavelength range and a low light absorption between 350 and 400 nm. These attractive features have been implemented in living zebrafish embryos to perform chromatic orthogonal photoactivation of two biologically active species controlling biological development with UV and blue-cyan light sources, respectively. © 2013 American Chemical Society. |