By monitoring the effect of deuterium decoupling on the decay of transverse 15N magnetization in D–15N spin pairs during multiple-refocusing echo sequences, we have determined fast D–D exchange rates kD and compared them with fast H–H exchange rates kH in tryptophan to determine the kinetic isotope effect as a function of pH and temperature.
N’hésitez pas à consulter également le communiqué de presse associé à cet article : Mesurer l’échange de protons !
References:
Kinetic isotope effects for fast deuterium and proton exchange rates
Estel Canet, Daniele Mammoli, Pavel Kaderavek, Philippe Pelupessy and Geoffrey Bodenhausen
Phys. Chem. Chem. Phys., 2016,18, 10144-10151
DOI: 10.1039/C5CP07459B
Kinetic isotope effects for fast deuterium and proton exchange rates