High-purity cardiomyocytes (CMs) derived from human induced pluripotent stem cells (hiPSCs) are promising for drug development and myocardial regeneration. However, most hiPSC-derived CMs morphologically and functionally resemble immature rather than adult CMs, which could hamper their application. Here, we obtained high-quality cardiac tissue-like constructs (CTLCs) by cultivating hiPSC-CMs on low-thickness aligned nanofibers made of biodegradable poly(D,L-lactic-co-glycolic acid) polymer. We show that multilayered and elongated CMs could be organized at high density along aligned nanofibers in a simple one-step seeding process, resulting in upregulated cardiac biomarkers and enhanced cardiac functions. When used for drug assessment, CTLCs were much more robust than the 2D conventional control.We also demonstrated the potential of CTLCs for modeling engraftments in vitro and treating myocardial infarction in vivo. Thus, we established a handy framework for cardiac tissue engineering, which holds high potential for pharmaceutical and clinical applications.
Pour plus d’information, consultez le communiqué de presse associé à cet article :
Réparer le cœur après un infarctus à l’aide de cellules souches !
References:
Human Pluripotent Stem Cell-Derived Cardiac Tissue-like Constructs for Repairing the Infarcted Myocardium
Junjun Li, Itsunari Minami, Motoko Shiozaki, Leqian Yu, Shin Yajima, Shigeru Miyagawa, Yuji Shiba, Nobuhiro Morone, Satsuki Fukushima, Momoko Yoshioka, Sisi Li, Jing Qiao, Xin Li, Lin Wang, Hidetoshi Kotera, Norio Nakatsuji, Yoshiki Sawa, Yong Chen, and Li Liu
Stem Cell Reports, 2017, 9, 1546–1559
DOI : 10.1016/j.stemcr.2017.09.007
Human Pluripotent Stem Cell-Derived Cardiac Tissue-like Constructs for Repairing the Infarcted Myocardium