Hyperpolarized water can selectively enhance NMR signals of rapidly exchanging protons in osteopontin (OPN), a metastasis-associated intrinsically disordered protein (IDP), at near-physiological pH and temperature. The transfer of magnetization from hyperpolarized water is limited to solventexposed residues and therefore selectively enhances signals in 1H-15N correlation spectra. Binding to the polysaccharide heparin was found to induce the unfolding of preformed structural elements in OPN.
N’hésitez pas à consuter le communiqué de presse associé à cet article : L’eau hyperpolarisée pour éclairer les protéines intrinsèquement désordonnées
References:
Investigation of Intrinsically Disordered Proteins through Exchange with Hyperpolarized Water
Dennis Kurzbach, Estel Canet, Andrea G. Flamm, Aditya Jhajharia, Emmanuelle M. M. Weber, Robert Konrat, and Geoffrey Bodenhausen
Angew. Chem. Int. Ed. 2017, 56, 389 –392
DOI: 10.1002/anie.201608903
Investigation of Intrinsically Disordered Proteins through Exchange with Hyperpolarized Water