Laboratoire P.A.S.T.E.U.R

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A novel diarylethene-based photoswitchable chelator for reversible release and capture of Ca2+ in aqueous media

Nadia Dozova,Guillaume Pousse, Bogdan Barnych, Jean-Maurice Mallet, Janine Cossy, Bernard Valeur, Pascal Plaza




A photochromic calcium chelator is reported to reversibly reduce its affinity for calcium upon photoswitching in aqueous media.



Fast and complete electrochemical conversion of solutes contained in microvolume water droplets

Electrochemistry Communications 86 (2018) 145–148


An elegant hanging-droplet or meniscus-based setup is proposed to carry out quantitative electrolyses from either an organic (hydroquinone) or an inorganic (permanganate) substrate. These examples validate the concept of using such easily accessible, fast (1–3 min) and low-cost operating conditions not only for preparative applications (electrosynthesis), but also for pedagogical purposes in minute samples.

Electrochemical switching fluorescence emission in rhodamine derivatives

Electrochimica Acta 260 (2018) 589-597


Three rhodamine derivatives exhibiting electrofluorochromic properties were investigated by cyclic voltammetry and UVeVis/fluorescence spectroelectrochemistry. Rhodamine 101 (Rh101, compound 1) was used as a reference model. In compound 2, the carboxylate anion of Rh101 was replaced by an alkyne moiety to allow further functionalization. The compound 3 was prepared from 2 by conversion of the alkyne to a triazole group bearing an alkyl chain with an alcohol function. These three rhodamine derivatives exhibited similar electrochemical behaviors. Their mono-electronic reductions produced the corresponding radical species which were stable on the time-scale of cyclic voltammetry. Additional reduction of electrogenerated radicals produced unstable anions which underwent subsequent chemical reaction, most likely protonation. Based on cyclic voltammetry investigations, absorption and fluorescence spectroelectrochemistry were then performed on compounds 1, 2, 3 and their parent reduced radicals 1a, 2a, 3a. UVeVis spectroelectrochemistry, combined with TD-DFT calculation, confirmed the formation of radicals upon mono-electronic reduction of starting rhodamines. Fluorescence spectroelectrochemistry showed that, contrary to their parent molecules, electrogenerated radicals were non-fluorescent. Electrochemical fluorescence extinction was successfully achieved with all studied compounds. Moreover, compound 1 underwent on/off switching between fluorescent and nonfluorescent states repeatedly. Also, recovery of fluorescence in compound 3 was observed, which open interesting opportunities for the development of versatile rhodamine-based probes.

Magnetic actuation of discrete liquid entities with a deformable paramagnetic liquid substrate

Angewandte Chemie (2017) 


Because miniaturization is accompanied by an increase in the surface-over-volume ratio, most strategies for controlled manipulation of small-sized drops on substrates have relied on exploiting interfacial energy gradients, making it possible to actuate drops with various stimuli, including electrical or optical ones, but requiring adequate stimulus-responsive systems. In contrast, magnetic actuation of deposited drops has mainly relied on volume forces exerted on the liquid to be transported, which is poorly efficient with conventional diamagnetic liquids such as water or oil, unless magnetosensitive particles are added. Here, we describe a new way to magnetically control the motion of discrete liquid entities, in an additive-free and surface tension-independent manner. Our strategy consists of using a paramagnetic liquid as a deformable substrate to direct, using a magnet, the motion of various floating liquid entities, ranging from naked drops to liquid marbles. We demonstrate that, in our configuration, it is the substrate deformation that mainly dictates the behaviour of the floating liquid entity. We show that a broad variety of liquids, including diamagnetic (water, oil) and nonmagnetic ones can be efficiently transported using the moderate magnetic field (≈ 50 mT) produced by a cost-effective, cmsized permanent magnet. Complex trajectories can be achieved in a reliable manner and multiplexing potential is demonstrated through on-demand drop fusion. Our paramagnetofluidic method advantageously works without any complex equipment nor electric power, in phase with the necessary development of robust and lowcost analytical and diagnostic fluidic devices.

Human Pluripotent Stem Cell-Derived Cardiac Tissue-like Constructs for Repairing the Infarcted Myocardium

Stem Cell Reports, 2017, 9, 1546–1559


High-purity cardiomyocytes (CMs) derived from human induced pluripotent stem cells (hiPSCs) are promising for drug development and myocardial regeneration. However, most hiPSC-derived CMs morphologically and functionally resemble immature rather than adult CMs, which could hamper their application. Here, we obtained high-quality cardiac tissue-like constructs (CTLCs) by cultivating hiPSC-CMs on low-thickness aligned nanofibers made of biodegradable poly(D,L-lactic-co-glycolic acid) polymer. We show that multilayered and elongated CMs could be organized at high density along aligned nanofibers in a simple one-step seeding process, resulting in upregulated cardiac biomarkers and enhanced cardiac functions. When used for drug assessment, CTLCs were much more robust than the 2D conventional control.We also demonstrated the potential of CTLCs for modeling engraftments in vitro and treating myocardial infarction in vivo. Thus, we established a handy framework for cardiac tissue engineering, which holds high potential for pharmaceutical and clinical applications.