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Bimodal X-ray and Infrared Imaging of an Organometallic Derivative of Praziquantel in Schistosoma mansoni

ChemBioChem 2016, 17, 1-5

 

An organometallic derivative of praziquantel was studied directly in worms by using inductively coupled plasma-mass spectrometry (ICP-MS) for quantification and synchrotronbased imaging. X-ray fluorescence (XRF) and IR absorption spectromicroscopy were used for the first time in combination to directly locate this organometallic drug candidate in schistosomes. The detection of both CO (IR) and Cr (XRF) signatures proved that the Cr(CO)3 core remained intact in the worms. Images showed a preferential accumulation at the worm’s tegument, consistent with a possible targeting of the calcium channel but not excluding other biological targets inside the worm.

Interview Clotilde Policar : France Culture

 

Clotilde Policar était l’invitée de l’émission “Divers aspects de la pensée contemporaine” sur France Culture. Elle parle de chimie bioinorganique, d’interdisciplinarité, des études au sein du Département de Chimie et de la Nuit Sciences et Lettres de l'ENS.

Kinetic isotope effects for fast deuterium and proton exchange rates

Phys. Chem. Chem. Phys., 2016,18, 10144-10151

 

By monitoring the effect of deuterium decoupling on the decay of transverse 15N magnetization in D–15N spin pairs during multiple-refocusing echo sequences, we have determined fast D–D exchange rates kD and compared them with fast H–H exchange rates kH in tryptophan to determine the kinetic isotope effect as a function of pH and temperature.

How to unveil self-quenched fluorophores and subsequently map the subcellular distribution of exogenous peptides

Scientific  Reports, 2016 Feb 3 ; 6:20237

 

Confocal laser scanning microscopy (CLSM) is the most popular technique for mapping the subcellular distribution of a fluorescent molecule and is widely used to investigate the penetration properties of exogenous macromolecules, such as cell-penetrating peptides (CPPs), within cells. Despite the membrane-association propensity of all these CPPs, the signal of the fluorescently labeled CPPs did not colocalize with the plasma membrane. We studied the origin of this fluorescence extinction and the overall consequence on the interpretation of intracellular localizations from CLSM pictures. We demonstrated that this discrepancy originated from fluorescence self-quenching. The fluorescence was unveiled by a “dilution” protocol, i.e. by varying the ratio fluorescent/non-fluorescent CPP. This strategy allowed us to rank with confidence the subcellular distribution of several CPPs, contributing to the elucidation of the penetration mechanism. More generally, this study proposes a broadly applicable and reliable method to study the subcellular distribution of any fluorescently labeled molecules.

Photophysical properties of single core multimodal probe for imaging (SCoMPI) in a membrane model and in cells

Dalton Transactions2016, 45, 2791

 

The spectroscopic properties of two luminescent Re(I) tricarbonyl complexes conjugated with two cell-penetrating peptides were examined. Fluorescence experiments and IR quanti!cation in membrane models and in cells showed unexpectedly strong luminescence enhancement for one of the complexes in a lipid environment.