Maître de conférence Sorbonne Université
ENS – Département de chimie
24 rue Lhomond, 75005 Paris
Email: manon.guille@ens.psl.eu
Phone: 0144322417
Office: E154
Member of ISE (International Society of Electrochemistry)
Member of BES (Bioelectrochemical Society)
Member of GFB (French Group of Bioelectrochemistry)
Member of SFC (French Society of Chemistry)
Education and Short Bio
2015 – Institut Universitaire de France Junior Member
2014 – Recipient of « Emergences Ville de Paris » Research Program
2013 – HDR Habilitation Defense, UPMC
2012 – CNRS Delegation (section 13)
2006 – Assistant Professor UPMC – UMR 8640
2005/2006 – Post-Doc « Marie Curie Intra-European Fellowship ».
«Electrochemistry and Interfaces Group» Prs. Patrick Unwin and Julie Macpherson. Warwick University, Coventry (UK).
2002/2005 – UPMC PhD – UMR 8640
2005 – Masters Degree in « Condensed Matter », UPMC
Research interests
-
- Bioelectrochemistry, Combined Electrochemical and Fluorescent Measurements, Cellular Secretion, Evanescent Wave Microscopy, Bioenergetics
Supervised students and post-doctorants
- 14 directions and co-directions
Teaching
- L1 « Structure / Reactivity » and « Chemistry of Solutions / Crystals »
- L2 « Thermodynamics and Chemical Kinetics »
- L3 « Thermodynamics and Electrochemistry »
- M1 « Chemistry of the Living »
Significant publications
- A. Rancillac, J. Rossier, M. Guille, XK.Tong, H. Geoffroy, C. Amatore, S. Arbault, E. Hamel, B. Cauli
“Glutamatergic control of microvascular tone by single GABA neurons in the cerebellum.”
Journal of Neuroscience, 2006, 26, 6997-7006.
- N. R. Wilson, M. Guille, I. Dumitrescu, V. R. Fernandez, N. C. Rudd, C. G. Williams, P. R. Unwin, J. V. Macpherson
“Assessment of the electrochemical behavior of two-dimensional networks of single-walled carbon nanotubes.”
Analytical Chemistry, 2006, 78, 7006-7015.
- C. Amatore, S. Arbault, M. Guille, F. Lemaître
“Electrochemical Monitoring of Single Cell Secretion: Vesicular Exocytosis and Oxidative Stress.”
Chemical Review, 2008, 108, 2585-2621.
- A. N. Patel, M. Guille-Collignon, M. A. O’Connell, W. O. Y. Hung, K. McKelvey, J. V. Macpherson, P. R. Unwin
“A new view of electrochemistry at highly oriented pyrolytic graphite (HOPG).”
Journal of American Chemical Society, 2012, 134, 20117–20130.
- Y. Li, A. Meunier, R. Fulcrand, C.Sella, C. Amatore, F. Lemaître, L.Thouin, M. Guille-Collignon
“Multi-Chambers Microsystem for Simultaneous ROS and RNS Electrochemical Detection at the Level of a Cellular Macrophages Population”
Electroanalysis, 2016, 28, 1-9
- X. Liu, A. Savy, S. Maurin, L. Grimaud, F. Darchen, D. Quinton, E. Labbé, O. Buriez, J. Delacotte, F. Lemaître, M. Guille-Collignon
« A dual functional electroactive and fluorescent probe for coupled measurements of vesicular exocytosis with high spatial and temporal resolution »
Angew. Chem. Int. Ed. 2017, 56, 2366-2370.
- H.-Y. Fu, D. Picot, Y. Choquet, G. Longatte, A. Sayegh, J. Delacotte, M. Guille-Collignon, F. Lemaître, F. Rappaport, F.-A. Wollman
» Redesigning the QA binding site of PSII allows reduction of exogenous quinones »
Nat. Commun. 2017, 8, 15274.
- X. Liu, L. Hu, N. Pan, L. Grimaud, E. Labbé, O. Buriez, J. Delacotte, F. Lemaître, M. Guille-Collignon
« Coupling Electrochemistry and TIRF-Microscopy with the Fluorescent False Neurotransmitter FFN102 Supports the Fluorescence Signals During Single Vesicle Exocytosis Detection »
Biophysical Chemistry 2018, 235, 48-55
- G. Longatte, A. Sayegh, J. Delacotte, F. Rappaport, F-A Wollman, M. Guille-Collignon, F. Lemaître
« Investigation of Photocurrents Resulting from Living Unicellular Algae Suspension with Quinones over Time »
Chemical Science 2018, 9, 8271
- J. Pandard, N. Pan, D. H. Ebene, T. Le Saux, E. Ait-Yahiatène, X. Liu, L. Grimaud, O. Buriez, E. Labbé, F. Lemaître, M. Guille-Collignon
» A Fluorescent False Neurotransmitter as a Dual Electrofluorescent Probe for Secretory Cell Models »
ChemPlusChem 2019
Publications
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2006
|
Glutamatergic control of microvascular tone by distinct GABA neurons in the cerebellum Article de journal Armelle Rancillac; Jean Rossier; Manon Guille; Xin-Kang Tong; Helene Geoffroy; Christian Amatore; Stephane Arbault; Edith Hamel; Bruno Cauli Journal of Neuroscience, 26 (26), p. 6997-7006, 2006, (Times Cited: 79). @article{,
title = {Glutamatergic control of microvascular tone by distinct GABA neurons in the cerebellum},
author = {Armelle Rancillac and Jean Rossier and Manon Guille and Xin-Kang Tong and Helene Geoffroy and Christian Amatore and Stephane Arbault and Edith Hamel and Bruno Cauli},
year = {2006},
date = {2006-01-01},
journal = {Journal of Neuroscience},
volume = {26},
number = {26},
pages = {6997-7006},
note = {Times Cited: 79},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Modelling release of nitric oxide in a slice of rat's brain: describing stimulated functional hyperemia with diffusion-reaction equations Article de journal A I Oleinick; C Amatore; M Guille; S Arbault; O V Klymenko; I Svir Mathematical Medicine and Biology-a Journal of the Ima, 23 (1), p. 27-44, 2006, (Times Cited: 12). @article{,
title = {Modelling release of nitric oxide in a slice of rat's brain: describing stimulated functional hyperemia with diffusion-reaction equations},
author = {A I Oleinick and C Amatore and M Guille and S Arbault and O V Klymenko and I Svir},
year = {2006},
date = {2006-01-01},
journal = {Mathematical Medicine and Biology-a Journal of the Ima},
volume = {23},
number = {1},
pages = {27-44},
note = {Times Cited: 12},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Nitric oxide release during evoked neuronal activity in cerebellum slices: Detection with platinized carbon-fiber microelectrodes Article de journal C Amatore; S Arbault; Y Bouret; B Cauli; M Guille; A Rancillac; J Rossier ChemPhysChem, 7 (1), p. 181–187, 2006. @article{Amatore:2006e,
title = {Nitric oxide release during evoked neuronal activity in cerebellum slices: Detection with platinized carbon-fiber microelectrodes},
author = {C Amatore and S Arbault and Y Bouret and B Cauli and M Guille and A Rancillac and J Rossier},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-31144464236&doi=10.1002%2fcphc.200500202&partnerID=40&md5=98d8f71d47f6a9199678a764ed645e15},
doi = {10.1002/cphc.200500202},
year = {2006},
date = {2006-01-01},
journal = {ChemPhysChem},
volume = {7},
number = {1},
pages = {181--187},
abstract = {Nitric oxide is an important biological messenger that particularly induces the relaxation of smooth muscle cells surrounding vessels, and, hence, controls the flow of blood. This mechanism is essential for brain function, and its fine control, termed functional hyperemia, is supposed to be realized by certain neurons that may release bursts of NO•. The aim of the present study is to examine the advantages of platinized carbon-fiber microelectrodes (5-7 μm tip diameter) for the direct and in situ electrochemical detection of NO• released by neurons into ex vivo cerebellum slices. After establishing the different analytical properties of the platinized carbon-fiber microelectrodes in vitro on NO• solutions at 50 nM to 1 mM concentration, they were characterized using DEA-NONOate solutions that chemically decompose into NO•, and therefore mimic the measurement of transient variations of NO• concentration in biological samples. This validated the present approach, so that direct, in situ ex vivo measurements of nitric oxide released by neurons in a rat cerebellar slice are presented and discussed. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Nitric oxide is an important biological messenger that particularly induces the relaxation of smooth muscle cells surrounding vessels, and, hence, controls the flow of blood. This mechanism is essential for brain function, and its fine control, termed functional hyperemia, is supposed to be realized by certain neurons that may release bursts of NO•. The aim of the present study is to examine the advantages of platinized carbon-fiber microelectrodes (5-7 μm tip diameter) for the direct and in situ electrochemical detection of NO• released by neurons into ex vivo cerebellum slices. After establishing the different analytical properties of the platinized carbon-fiber microelectrodes in vitro on NO• solutions at 50 nM to 1 mM concentration, they were characterized using DEA-NONOate solutions that chemically decompose into NO•, and therefore mimic the measurement of transient variations of NO• concentration in biological samples. This validated the present approach, so that direct, in situ ex vivo measurements of nitric oxide released by neurons in a rat cerebellar slice are presented and discussed. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA. |
Regulation of exocytosis in chromaffin cells by Trans-insertion of lysophosphatidylcholine and arachidonic acid into the outer leaflet of the cell membrane Article de journal C Amatore; S Arbault; Y Bouret; M Guille; F Lemaître; Y Verchier ChemBioChem, 7 (12), p. 1998–2003, 2006. @article{Amatore:2006i,
title = {Regulation of exocytosis in chromaffin cells by Trans-insertion of lysophosphatidylcholine and arachidonic acid into the outer leaflet of the cell membrane},
author = {C Amatore and S Arbault and Y Bouret and M Guille and F Lema\^{i}tre and Y Verchier},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-33845431788&doi=10.1002%2fcbic.200600194&partnerID=40&md5=ee09aa44f07bb791da49cab4b5ce936a},
doi = {10.1002/cbic.200600194},
year = {2006},
date = {2006-01-01},
journal = {ChemBioChem},
volume = {7},
number = {12},
pages = {1998--2003},
abstract = {Vesicular exocytosis is an important complex process in the communication between cells in organisms. It controls the release of chemical and biochemical messengers stored in an emitting cell. In this report, exocytosis is studied amperometrically (at carbon fiber ultramicroelectrodes) at adrenal chromaffin cells, which release catecholamines after appropriate stimulation, while testing the effects due to trans-insertion of two exogenous compounds (lysophosphatidylcholine (LPC) and arachidonic acid (AA)) on the kinetics of exocytotic events. Amperometric analyses showed that, under the present conditions (short incubation times and micromolar LPC or AA solutions), LPC favors catecholamine release (rate, event frequency, charge released) while AA disfavors the exocytotic processes. The observed kinetic features are rationalized quantitatively by considering a stalk model, for the fusion pore formation, and the physical constraints applied to the cell membrane by the presence of small fractions of LPC and AA diluted in its external leaflet (trans-insertion). We also observed that the detected amount of neurotransmitters in the presence of LPC was larger than under control conditions, while the opposite trend is observed with AA. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Vesicular exocytosis is an important complex process in the communication between cells in organisms. It controls the release of chemical and biochemical messengers stored in an emitting cell. In this report, exocytosis is studied amperometrically (at carbon fiber ultramicroelectrodes) at adrenal chromaffin cells, which release catecholamines after appropriate stimulation, while testing the effects due to trans-insertion of two exogenous compounds (lysophosphatidylcholine (LPC) and arachidonic acid (AA)) on the kinetics of exocytotic events. Amperometric analyses showed that, under the present conditions (short incubation times and micromolar LPC or AA solutions), LPC favors catecholamine release (rate, event frequency, charge released) while AA disfavors the exocytotic processes. The observed kinetic features are rationalized quantitatively by considering a stalk model, for the fusion pore formation, and the physical constraints applied to the cell membrane by the presence of small fractions of LPC and AA diluted in its external leaflet (trans-insertion). We also observed that the detected amount of neurotransmitters in the presence of LPC was larger than under control conditions, while the opposite trend is observed with AA. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. |
2003
|
Dynamics of full fusion during vesicular exocytotic events: Release of adrenaline by chromaffin cells Article de journal C Amatore; S Arbault; I Bonifas; Y Bouret; M Erard; M Guille ChemPhysChem, 4 (2), p. 147–154, 2003. @article{Amatore:2003d,
title = {Dynamics of full fusion during vesicular exocytotic events: Release of adrenaline by chromaffin cells},
author = {C Amatore and S Arbault and I Bonifas and Y Bouret and M Erard and M Guille},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037450055&doi=10.1002%2fcphc.200390024&partnerID=40&md5=fba834474dafda276b2a40772d6c8796},
doi = {10.1002/cphc.200390024},
year = {2003},
date = {2003-01-01},
journal = {ChemPhysChem},
volume = {4},
number = {2},
pages = {147--154},
abstract = {Vesicular exocytosis is important in the communication between cells in complex organisms. It controls the release of specific chemical or biochemical messengers stored in the emitting cell, which elicit a response upon detection by the target cells. Secretion of a messenger molecule (a neurotransmitter) was measured electrochemically, which allowed the quantification of cellular events and the validation of current physicochemical models. This model led us to formulate predictions about the occurrence and kinetics of vesicular exocytotic events based on the physicochemical meaning of its key parameters. These predictions were tested successfully through a series of experiments on chromaffin cells, involving changes of osmotic conditions, presence of trivalent ions and cholesterol-induced structuring of the cell plasmic membrane.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Vesicular exocytosis is important in the communication between cells in complex organisms. It controls the release of specific chemical or biochemical messengers stored in the emitting cell, which elicit a response upon detection by the target cells. Secretion of a messenger molecule (a neurotransmitter) was measured electrochemically, which allowed the quantification of cellular events and the validation of current physicochemical models. This model led us to formulate predictions about the occurrence and kinetics of vesicular exocytotic events based on the physicochemical meaning of its key parameters. These predictions were tested successfully through a series of experiments on chromaffin cells, involving changes of osmotic conditions, presence of trivalent ions and cholesterol-induced structuring of the cell plasmic membrane. |
65 Entrées « ‹ 5 de 5
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