Interactions between proteins play an essential role in metabolic and signaling pathways, cellular processes and organismal systems. We report the development of splitFAST, a fluorescence complementation system for the visualization of transient protein-protein interactions in living cells. Engineered from the fluorogenic reporter FAST (Fluorescence-Activating and absorption-Shifting Tag), which specifically and reversibly binds fluorogenic hydroxybenzylidene rhodanine (HBR) analogs, splitFAST displays rapid and reversible complementation, allowing the real-time visualization of both the formation and the dissociation of a protein assembly.
Press release available :
A split fluorescent reporter with rapid and reversible complementation
Alison G. Tebo and Arnaud Gautier*
PASTEUR, Département de chimie, École normale supérieure, PSL University, Sorbonne Université́, CNRS, 24 Rue Lhomond, 75005 Paris, France
Nature Communications 10, 2822 (2019)
DOI : 10.1038/s41467-019-10855-0