Nanoelectrodes for Determination of Reactive Oxygen and Nitrogen Species inside Murine Macrophages

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Micrometer-sized electrodes coated with Pt black have previously been used for selective and sensitive detection of ROS/RNS in biological systems.  However, determining ROS/RNS inside macrophages, requires smaller, i.e., nanometer-sized sensors.  In this article, the methodologies have been extended to the fabrication and characterization of Pt/Pt black nanoelectrodes.  Electrodes with the metal surface flush with glass insulator, most suitable for quantitative


Reactive oxygen and nitrogen species (ROS and RNS) produced by macrophages are essential for protecting a human body against bacteria and viruses through digestion of ingested bodies in specific vacuoles. However, the issue concerning the potential leakage of ROS/RNS from vacuoles has been raised as a tentative explanation to some illness (e.g., gout). The purpose of this work was to investigate quantitatively and kinetically this issue.  

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 Y. Wang, J-M Noël, J. Velmurugan, W. Nogalaa, M. Mirkin, C. Lu,, M. Guille Collignon, F. Lemaître and C. Amatore. PNAS, 2012, 109, 11534-11539. (Abstract + PDF)