Towards high throughput production of artificial egg oocytes using microfluidics

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TitreTowards high throughput production of artificial egg oocytes using microfluidics
Type de publicationJournal Article
Nouvelles publications2011
AuteursJimenez, AM, Roche M, Pinot M, Panizza P, Courbin L, Gueroui Z
JournalLab on a Chip
Volume11
Fascicule3
Pagination429-434
Numéro1473-0197
Accession NumberISI:000286326700011
Résumé

The production of micron-size droplets using microfluidic tools offers new opportunities to carry out biological assays in a controlled environment. We apply these strategies by using a flow-focusing microfluidic device to encapsulate Xenopus egg extracts, a biological system recapitulating key events of eukaryotic cell functions in vitro. We present a method to generate monodisperse egg extract-in-oil droplets and use high-speed imaging to characterize the droplet pinch-off dynamics leading to the production of trains of droplets. We use fluorescence microscopy to show that our method does not affect the biological activity of the encapsulated egg extract by observing the self-organization of microtubules and actin filaments, two main biopolymers of the cell cytoskeleton, encapsulated in the produced droplets. We anticipate that this assay might be useful for quantitative studies of biological systems in a confined environment as well as high throughput screenings for drug discovery.

URL<Go to ISI>://000286326700011
DOI10.1039/c0lc00046a
Importer un fichierTowards high throughput production of artificial egg oocytes using microfluidics
Unité de rattachement: 
UMR 8640