Resonant response to temperature modulation for enzymatic dynamics characterization

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TitreResonant response to temperature modulation for enzymatic dynamics characterization
Type de publicationJournal Article
Nouvelles publications2009
AuteursBerthoumieux, H, Antoine C, Jullien L, Lemarchand A
JournalPhysical Review E
Volume79
Fascicule2
Année de publicationFeb
Numéro1539-3755
Accession NumberISI:000263807300085
Résumé

We consider enzymes involved in a three-state Michaelis-Menten kinetics and submitted to well-chosen temperature modulations of small amplitude. From the first-order amplitudes of concentration oscillations, we design a response function that is maximum for targeted values of the chemical relaxation times. This resonant function can be used to screen a large set of enzymes and identify the one governed by the desired kinetics. The method gives access to all the dynamical parameters of the targeted enzyme without resorting to a fit. We show how to estimate the precision of this parameter determination and give some hints for experimental validation.

URL<Go to ISI>://000263807300085
DOI10.1103/PhysRevE.79.021906
Importer un fichierResonant response to temperature modulation for enzymatic dynamics characterization
Unité de rattachement: 
UMR 8640