Photoactivation of the CreER(T2) Recombinase for Conditional Site-Specific Recombination with High Spatiotemporal Resolution

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TitrePhotoactivation of the CreER(T2) Recombinase for Conditional Site-Specific Recombination with High Spatiotemporal Resolution
Type de publicationJournal Article
Nouvelles publications2010
AuteursSinha, DK, Neveu P, Gagey N, Aujard I, Le Saux T, Rampon C, Gauron C, Kawakami K, Leucht C, Bally-Cuif L, Volovitch M, Bensimon D, Jullien L, Vriz S
JournalZebrafish
Volume7
Fascicule2
Pagination199-204
Année de publicationJun
Numéro1545-8547
Accession NumberISI:000278544100009
Résumé

We implemented a noninvasive optical method for the fast control of Cre recombinase in single cells of a live zebrafish embryo. Optical uncaging of the caged precursor of a nonendogeneous steroid by one-or two-photon illumination was used to restore Cre activity of the CreER(T2) fusion protein in specific target cells. This method labels single cells irreversibly by inducing recombination in an appropriate reporter transgenic animal and thereby can achieve high spatiotemporal resolution in the control of gene expression. This technique could be used more generally to investigate important physiological processes (e.g., in embryogenesis, organ regeneration, or carcinogenesis) with high spatiotemporal resolution (single cell and 10-min scales).

URL<Go to ISI>://000278544100009
DOI10.1089/zeb.2009.0632
Importer un fichierPhotoactivation of the CreER(T2) Recombinase for Conditional Site-Specific Recombination with High Spatiotemporal Resolution
Unité de rattachement: 
UMR 8640