D-Amino Acid Detection in Peptides by MALDI-TOF-TOF

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TitleD-Amino Acid Detection in Peptides by MALDI-TOF-TOF
Publication TypeJournal Article
Year of Publication2009
AuthorsSachon, E, Clodic G, Galanth C, Amiche M, Ollivaux C, Soyez D, Bolbach G
JournalAnalytical Chemistry
Volume81
Issue11
Pagination4389-4396
Date PublishedJun
ISBN Number0003-2700
Accession NumberISI:000266601800032
Abstract

Detection of a D-amino acid residue in natural peptides by mass spectrometry remains a challenging task, as this post-translational modification does not induce any change in molecular mass. To our knowledge, the present article is the first report using matrix-assisted laser desorption/ionization (MALDI) for the discrimination and the quantification of peptide isomers. In this work, we used synthetic hepta- and decapeptides of biological relevance and their isomers. All-L sequences and some isomers containing a D-residue in various positions were analyzed.

URL<Go to ISI>://000266601800032
DOI10.1021/ac9002886
Short TitleD-Amino Acid Detection in Peptides by MALDI-TOF-TOF
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