Synthetic biotinylated tetra beta(1 -> 5) galactofuranoside for in vitro aspergillosis diagnosis

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TitreSynthetic biotinylated tetra beta(1 -> 5) galactofuranoside for in vitro aspergillosis diagnosis
Type de publicationJournal Article
Nouvelles publications2011
AuteursCattiaux, L, Sendid B, Collot M, Machez E, Poulain D, Mallet J-M
JournalBioorganic & Medicinal Chemistry
Volume19
Fascicule1
Pagination547-555
Année de publicationJan
Numéro0968-0896
Accession NumberISI:000285724800056
Résumé

The synthesis of a tetra beta(1 -> 5) galactofuranoside was achieved using a thioglycoside donor with a methyl tert-butyl phenyl thio leaving group. This tetrasaccharide was conjugated to biotin and validated as antigen with the monoclonal antibody used for clinical detection of Aspergillus fumigatus galactomannan on streptavidin-coated microplates. Then we have shown its ability to detect antibodies associated with A. fumigatus induced disease by using sera from patients with Allergic broncho-pulmonary aspergillosis (ABPA) and correlated the results of antibody detection with those gained with a commercially available diagnostic test. (C) 2010 Elsevier Ltd. All rights reserved.

URL<Go to ISI>://000285724800056
DOI10.1016/j.bmc.2010.10.062
Importer un fichierSynthetic biotinylated tetra beta(1 -> 5) galactofuranoside for in vitro aspergillosis diagnosis
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