Permeabilization of Lipid Membranes and Cells by a Light-Responsive Copolymer

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TitrePermeabilization of Lipid Membranes and Cells by a Light-Responsive Copolymer
Type de publicationJournal Article
Nouvelles publications2010
AuteursSebai, SC, Cribier S, Karimi A, Massotte D, Tribet C
Année de publicationSep
Accession NumberISI:000281354000058

Membrane permeabilization is achieved via numerous techniques involving the use of molecular agents such as peptides used in antimicrobial therapy. Although high efficiency is reached, the permeabilization mechanism remains global with a noticeable lack of control. To achieve localized control and more gradual increase in membrane perturbation, we have developed hydrophobically modified poly(acrylic acid) amphiphilic copolymers with lightresponsive azobenzene hydrophobic moieties. We present evidence for light triggered membrane permeabilization in the presence azobenzene-modified polymers (A M Ps). Exposure to UV or blue light reversibly switches the polarity of the azobenzene (cis trans isomerization) in AMPs, hence controlling AMP-loaded lipid vesicles permeabilization via in situ activation. Release of encapsulated probes was studied by microscopy on isolated AMP-loaded giant unilamellar vesicles (pol-GU Vs). We show that in pH and ionic strength conditions that are biologically relevant pol-GUVs are kept impermeable when they contain predominantly cis-AMPs but become leaky with no membrane breakage upon exposure to blue light due to AMPs switch to a trans-apolar state. In addition, we show that AM Ps induce destabilization of plasma membranes when added to mammal cells in their trans-apolar state, with no loss of cell viability. These features make AM Ps promising tools for remote control of cell membrane permeabilization in mild conditions.

URL<Go to ISI>://000281354000058
Importer un fichierPermeabilization of Lipid Membranes and Cells by a Light-Responsive Copolymer
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