Spatially-Controlled Protein Crystallization in Microfluidic Chambers

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TitreSpatially-Controlled Protein Crystallization in Microfluidic Chambers
Type de publicationJournal Article
Nouvelles publications2014
AuteursLonguet C, Yamada A, Chen Y, Baigl D, Fattaccioli J
JournalJournal of Crystal Growth
Volume386
Pagination179-182
Année de publication2014
ISBN00220248
Résumé

We present a simple microfluidic device able to trigger the nucleation of the crystals at specific locations on the microchip for the statistical study of protein crystallization. The microsystem is an array of independent PDMS microchambers connected to a fluid-dispensing channel. The chambers are filled with a crystallizing aqueous protein solution and then sealed with a fluorinated oil phase. Each chamber presents a small oil/water interface at the connection with the main channel. The crystals most likely grow near the interface, allowing a microscopic observation of the nucleation events at specific positions on the chip. For the sake of demonstration, the method is applied the crystallization of HEW lysozyme.

DOI10.1016/j.jcrysgro.2013.10.011
Importer un fichierJournal of Crystal Growth
Unité de rattachement: 
UMR 8640