UMR 8640 : Electrochimie

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Interview Christian Amatore : The Electrochemical Society

Christian Amatore has given an interview at The Electrochemical Society in the ECS Podcast context.

 

Astrocyte-derived adenosine is central to the hypnogenic effect of glucose

Scientific Reports 6, Article number: 19107 (2016)

 

Sleep has been hypothesised to maintain a close relationship with metabolism. Here we focus on the brain structure that triggers slow-wave sleep, the ventrolateral preoptic nucleus (VLPO), to explore the cellular and molecular signalling pathways recruited by an increase in glucose concentration. We used infrared videomicroscopy on ex vivo brain slices to establish that glucose induces vasodilations specifically in the VLPO via the astrocytic release of adenosine. Real-time detection by in situ purine biosensors further revealed that the adenosine level doubles in response to glucose, and triples during the wakefulness period. Finally, patch-clamp recordings uncovered the depolarizing effect of adenosine and its A2A receptor agonist, CGS-21680, on sleep-promoting VLPO neurons. Altogether, our results provide new insights into the metabolically driven release of adenosine. We hypothesise that adenosine adjusts the local energy supply to local neuronal activity in response to glucose. This pathway could contribute to sleep-wake transition and sleep intensity.

 

Three-electrode analytical and preparative electrochemistry in micro-volume hanging droplets

Electrochem. Commun. 54 (2015) 41

 

Three-electrode micro-cells equipped with a conventional reference electrode (SCE) were easily constructed based on micro-volume droplets suspended by capillary forces to the fritted glass of the SCE bridge. Working and counter electrodes were simply inserted through the droplet surface, allowing classical electrochemistry to be readily performed in minute samples.

 

 

Interactions between Human Antibodies and Synthetic Conformational Peptide Epitopes: Innovative Approach for Electrochemical Detection of Biomarkers of Multiple Sclerosis at Platinum Electrodes

The detection of human antibodies of Multiple Sclerosis patients was investigated based on the electrochemical oxidation of a synthetic antigenic probe, a glycopeptide Fc-CSF114(Glc) bearing a ferrocenyl moiety. Preliminary tests using human sera from patients and healthy donors validated this new approach aimed at developing innovative and fast diagnostic tools, based on electrochemical synthetic antigenic probes.

Monitoring and Quantifying the Passive Transport of Molecules Through Patch–Clamp Suspended Real and Model Cell Membranes

Transport of active molecules across biological membranes is a central issue for the success of many pharmaceutical strategies. Herein, we combine the patch-clamp principle with amperometric detection for monitoring fluxes of redox-tagged molecular species across a suspended membrane patched from a macrophage.