The role of intrinsically disordered proteins in transcriptional regulation

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The regulation of the cellular machinery relies on a plethora of DNA transcription factors (TFs)[1-4]. Their understanding is therefore of widespread medicinal, pharmacological, and biological interest, but despite long-standing research projects essential properties remain ununderstood. A peculiar example is the breast cancer susceptibility protein 1 (BRCA1), which upon mutation perturbs the transcriptional regulation and can cause breast cancer. Interestingly, BRCA1 is an IDP, an intrinsically disordered protein. To understand the implications of IDPs in transcriptional regulation and I propose to employ dissolution DNP. In this talk, I will give an overview over the efforts that have so far been undertaken to combine NMR of TFs and IDPs with D-DNP and give an outlook over future endeavors.

1. Wechsler, D. S.; Papoulas, O.; Dang, C. V.; Kingston, R. E., Mol Cell Biol 1994,14 (6), 4097-4107.

2. Brownlie, P.; Ceska, T. A.; Lamers, M.; Romier, C.; Stier, G.; Teo, H.; Suck, D., Structure 1997, 5 (4), 509-520.

3. Paull, T. T.; Cortez, D.; Bowers, B.; Elledge, S. J.; Gellert, M., Proc Natl Acad Sci U S A 2001, 98 (11), 6086-91.

4. Hilser, V. J.; Thompson, E. B., J. Biol. Chem. 2012, 286, 39675-39682.

 

 

Sorbonne Université, room 23-13-109

Mardi 23 Octobre 2018 12:30
Dr. KURZBACH Dennis
Séminaire Thématique
Unité de rattachement: 
UMR 7203
Equipe de rattachement 7203: 
Equipe 3