Graftable SCoMPIs enable the labeling and X-ray fluorescence imaging of proteins

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Graftable SCoMPIs enable the labeling and X-ray fluorescence imaging of proteins, Chem. Sci.2018, 9, 4483-4487

 

Bio-imaging enables the direct visualization of labeled biomolecules of interest, offering valuable insights into biological processes. Fluorescence microscopy is widely used and offers numerous advantages, including good spatial resolution and availability of numerous probes and tags with high quantum yield and diverse spectroscopic properties. However, fluorescence techniques suffer from some drawbacks such as photobleaching. Quantum yields are dependent on the environment, with large variations upon polarity or possible quenching at high concentrations. For that reason, absolute quantification using classical fluorescence can be tricky.

 

 

In conclusion, the results presented herein showed that it was possible to label and map by X-ray fluorescence an exogenous protein in CHO cells and membrane bound proteins in A549 cells, which establishes the high sensitivity of Re-based probes for X-ray imaging. This proof of concept offers new perspectives in XRF imaging, for which so far no specific graftable tags were available. This third modality of SCoMPI opens new opportunities for correlative imaging.

 

Pour plus d'informations, consultez le communiqué de presse associé à cet article : Des complexes de rhénium pour cartographier le vivant !

 

Résumé: 

Chem. Sci.2018, 9, 4483-4487

 

Bio-imaging techniques alternative to fluorescence microscopy are gaining increasing interest as complementary tools to visualize and analyze biological systems. Among them, X-ray fluorescence microspectroscopy provides information on the local content and distribution of heavy elements (Z > 14) in cells or biological samples. In this context, similar tools to those developed for fluorescence microscopy are desired, including chemical probes or tags. In this work, we study rhenium complexes as a convenient and sensitive probe for X-ray fluorescence microspectroscopy. We demonstrate their ability to label and sense exogenously incubated or endogenous proteins inside cells.

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Graftable SCoMPIs enable the labeling and X-ray fluorescence imaging of proteins

 

Sarah Hostachy, Marie Masuda, Takayuki Miki, Itaru Hamachi, Sandrine Sagan, Olivier Lequin, Kadda Medjoubi, Andrea Somogyi, Nicolas Delsuc and Clotilde Policar

 

Chem. Sci., 2018, 9, 4483-4487

 

DOI : 10.1039/c8sc00886h