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Dr. AMATORE Christian
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RAZAKA
mada
BONELLA Sara
Physics department, University Rome 1 "La Sapienza"
MIRKIN Michael
Queens College - Department of chemistry and Biochemistry - New York - USA
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Bousquet, David, Coudert François-Xavier, Fossati Alexandre G. J., Neimark Alexander V., Fuchs Alain H., and Boutin Anne
The Journal of Chemical Physics,
Volume 138,
p. 174706,
2013
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Mme GUILLE COLLIGNON Manon
Thursday 6 June 2013 10:00
E012 (ENS, département de Chimie)
Home Publications Self-loading and cell culture in one layer microfluidic devices
Self-loading and cell culture in one layer microfluidic devices
| Title | Self-loading and cell culture in one layer microfluidic devices |
| Publication Type | Journal Article |
| Year of Publication | 2009 |
| Authors | Wang, L, Ni XF, Luo CX, Zhang ZL, Pang DW, Chen Y |
| Journal | Biomedical Microdevices |
| Volume | 11 |
| Issue | 3 |
| Pagination | 679-684 |
| Date Published | Jun |
| ISBN Number | 1387-2176 |
| Accession Number | ISI:000265440300018 |
| Abstract | We report on a simple method for self loading and culture of mammalian cells in microfluidic multi-chambers for high throughput screening. The device was obtained by using one layer soft lithography with polydimethylsiloxane (PDMS) and thermal bonding on a glass slide. Self loading of cell suspension could be possible after degassing of the PDMS device for 30 min. Both cell loading efficiency and cell proliferation behaviors have been analyzed with triangle chambers of different sizes, all connected to the main flow channels with small entrances. We found that the number of cells loaded into the micro-chamber increased with the side length of the triangle, showing well size dependence and that self loading at a single cell level was possible for small chambers. For large chambers, the cell area density after loading and proliferation is however quite heterogeneous. For demonstration, HeLa cell growth behavior has been followed for 11 days until the total area of the largest chambers was fully filled. |
| URL | <Go to ISI>://000265440300018 |
| DOI | 10.1007/s10544-008-9278-0 |
| Short Title | Self-loading and cell culture in one layer microfluidic devices |