Cell-penetrating Peptides with Intracellular Actin-remodeling Activity in Malignant Fibroblasts

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TitleCell-penetrating Peptides with Intracellular Actin-remodeling Activity in Malignant Fibroblasts
Publication TypeJournal Article
Year of Publication2010
AuthorsDelaroche D, Cantrelle FX, Subra F, Van Heijenoort C, Guittet E, Jiao CY, Blanchoin L, Chassaing G, Lavielle S, Auclair C, Sagan S
JournalJournal of Biological Chemistry
Volume285
Issue10
Pagination7712-7721
Date PublishedMar
ISBN Number0021-9258
Accession NumberISI:000275415600080
Abstract

Cell-penetrating peptides can cross cell membranes and are commonly seen as biologically inert molecules. However, we found that some cell-penetrating peptides could remodel actin cytoskeleton in oncogene-transformed NIH3T3/EWS-Fli cells. These cells have profound actin disorganization related to their tumoral transformation. These arginine- and/or tryptophan-rich peptides could cross cell membrane and induce stress fiber formation in these malignant cells, whereas they had no perceptible effect in non-tumoral fibroblasts. In addition, motility (migration speed, random motility coefficient, wound healing) of the tumor cells could be decreased by the cell-permeant peptides. Although the peptides differently influenced actin polymerization in vitro, they could directly bind monomeric actin as determined by NMR and calorimetry studies. Therefore, cell-penetrating peptides might interact with intracellular protein partners, such as actin. In addition, the fact that they could reverse the tumoral phenotype is of interest for therapeutic purposes.

URL<Go to ISI>://000275415600080
DOI10.1074/jbc.M109.045872
Short TitleCell-penetrating Peptides with Intracellular Actin-remodeling Activity in Malignant Fibroblasts
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