Asymmetrical stress generated by the erythrocyte lipid flippase triggers multiple bud formation on the surface of spherical giant liposomes

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TitleAsymmetrical stress generated by the erythrocyte lipid flippase triggers multiple bud formation on the surface of spherical giant liposomes
Publication TypeJournal Article
Year of Publication2010
AuthorsEzanno P, Cribier S, Devaux PF
JournalEuropean Biophysics Journal with Biophysics Letters
Volume39
Issue8
Pagination1277-1280
Date PublishedJul
ISBN Number0175-7571
Accession NumberISI:000279194500015
Abstract

Proteo-giant liposomes were electroformed from a mixture of lecithin vesicles and inside-out vesicles from erythrocytes. After addition of Mg-ATP in the vicinity of the proteo-giant liposomes, small buds appeared on the liposome surfaces, which-via an increase in lipids in the outer monolayer-demonstrated the active transport of lipids from the inner to the outer monolayer, indicating flippase activity.

URL<Go to ISI>://000279194500015
DOI10.1007/s00249-009-0557-3
Short TitleAsymmetrical stress generated by the erythrocyte lipid flippase triggers multiple bud formation on the surface of spherical giant liposomes
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