Thesis prize 2014 from the Division Chimie-Physique de la Société Chimique de France

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Sylvain Clède, thesis prize 2014 from the Division Chimie-Physique de la Société Chimique de France
"From metal carbonyls to single core multimodal probes for imaging"


Thesis supervized by Clotilde Policar and François Lambert


Sylvain Clède will be giving a conference on his work on October 17th 2014 for the "Journées Francophones des Jeunes Physico-Chimistes" (link : http://www.cpps.u-psud.fr/?page_id=340).

His work deals with the development of a Single Core Multimodal probe for Imaging (SCoMPI). It is based on a unique molecular core, with a tris-carbonyl Rhenium and a ligand L bearing low-pi orbitals and being IR-responsive through the CO moieties and luminescent through the Re-L interaction. This probe enabled the tracking of an estrogen in correlative spectroscopy (IR and luminescent). The effect of substituants on its intracellular uptake and cytotoxicity was investigated. The originality of this work relies on the systematic application of IR-imaging, which is quite attractive: no photobleaching, organelle tracking without the need for label. The SCoMPI are probes of great potential enabling correlative imaging for a better understanding of biological phenonema.

Publications

Clède, S.; Policar, C. Metal Carbonyl Units for Vibrational and Luminescence Imaging: Towards Multimodality, Chem. - Eur. J. Review 2014, in revision.

Clède, S.; Lambert, F.; Saint-Fort, R.; Plamont, M.-A.; Bertrand, H.; Vessières, A.; Policar, C. Influence of the Side Chain Length on the Cellular Uptake and the Cytotoxicity of Rhenium tris-Carbonyl Derivatives: a Bimodal Infrared and Luminescence Quantitative Study, Chem. - Eur. J. 2014, DOI: 10.1002/chem.201402471R1.

Clède, S.; Sandt, C.; Policar, C. FTIR spectromicroscopy to identify cell organelles. Correlation with fluorescence staining in MCF-7 breast cancer cells, Appl. Spectrosc. 2014, 68, 113.

Clède, S.; Lambert, F.; Sandt, C.; Kascakova, S.; Unger, M.; Harté, E.; Plamont, M.-A.; Saint-Fort, R.; Deniset-Besseau, A.; Gueroui, Z.; Hirschmugl, C.; Lecomte, S.; Dazzi, A.; Vessières, A.; Policar, C. Detection of an Estrogen Derivative in Two Breast Cancer Cell Lines using a Single Core Multimodal Probe for Imaging (SCoMPI) Imaged by a Panel of Luminescent and Vibrational Techniques, Analyst 2013, 138, 5627.

Clède, S.; Lambert, F.; Sandt, C.; Gueroui, Z.; Delsuc, N.; Dumas, P.; Vessières A.; Policar, C. Synchrotron radiation FTIR detection of a metal-carbonyl tamoxifen analog. Correlation with luminescence microscopy to study its subcellular distribution, Biotechnol. Adv. 2013, 31, 393.

Clède, S.; Lambert, F.; Sandt, C.; Gueroui, Z.; Réfrégiers, M.; Plamont, M.-A.; Dumas, P.; Vessières, A.; Policar, C. A rhenium tris-carbonyl derivative as a single core multimodal probe for imaging (SCoMPI) combining infrared and luminescent properties, Chem. Commun. 2012, 48(62), 7729.

Bertrand, H.C.; Clède, S.; Guillot, R.; Lambert, F.; Policar, C., Luminescence Modulations of Rhenium Tricarbonyl Complexes Induced by Structural Variations, Inorg. Chem. 2014, 53, 6204.

Policar, C.; Waern, J. B.; Plamont, M.-A.; Clède, S.; Mayet, C.; Prazeres, R.; Ortega, J.-M.; Vessières, A.; Dazzi, A. Subcellular IR Imaging of a Metal–Carbonyl Moiety Using Photothermally Induced Resonance, Angew. Chem. Int. Ed. 2011, 860.

Mattson, E. C.; Unger, M.; Clède, S.; Lambert, F.; Policar, C.; Imtiaz, A.; D’Souza, R.; Hirschmugl, C. Toward Optimal Spatial and Spectral Quality in Widefield Infrared Spectromicroscopy of IR Labelled Single Cells, Analyst 2013, 138, 5610.

 



 

Last Name: 
M. CLEDE Sylvain
Monday 1 September 2014 10:00
Unit: 
Departement